EA200400833A1 - Способ выделения и очистки поксвирусов из инфицированных клеток - Google Patents

Способ выделения и очистки поксвирусов из инфицированных клеток

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Publication number
EA200400833A1
EA200400833A1 EA200400833A EA200400833A EA200400833A1 EA 200400833 A1 EA200400833 A1 EA 200400833A1 EA 200400833 A EA200400833 A EA 200400833A EA 200400833 A EA200400833 A EA 200400833A EA 200400833 A1 EA200400833 A1 EA 200400833A1
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EA
Eurasian Patent Office
Prior art keywords
virus
infected cells
present
poxviruses
isolation
Prior art date
Application number
EA200400833A
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English (en)
Other versions
EA006485B1 (ru
Inventor
Карл Хеллер
Ютта Крамер
Original Assignee
Бавариан Нордик А/С
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Бавариан Нордик А/С filed Critical Бавариан Нордик А/С
Publication of EA200400833A1 publication Critical patent/EA200400833A1/ru
Publication of EA006485B1 publication Critical patent/EA006485B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Настоящее изобретение относится к способу выделения поксвирусов, а в частности, модифицированного вируса коровьей оспы Анкара (MVA) из инфицированных клеток. В соответствии с настоящим изобретением инфицированные вирусом клетки подвергают гомогенизации при высоком давлении с получением вируссодержащего гомогената. Вируссодержащий гомогенат может быть подвергнут по крайней мере одной стадии очистки с получением обогащенной поксвирусом фракции. Настоящее изобретение также относится к вируссодержащей фракции и поксвируссодержащему гомогенату, полученным способом настоящего изобретения.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200400833A 2001-12-20 2002-12-13 Способ выделения и очистки поксвирусов из инфицированных клеток EA006485B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101928 2001-12-20
PCT/EP2002/014179 WO2003054175A1 (en) 2001-12-20 2002-12-13 Method for the recovery and purification of poxviruses from infected cells

Publications (2)

Publication Number Publication Date
EA200400833A1 true EA200400833A1 (ru) 2004-12-30
EA006485B1 EA006485B1 (ru) 2005-12-29

Family

ID=8160920

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200400833A EA006485B1 (ru) 2001-12-20 2002-12-13 Способ выделения и очистки поксвирусов из инфицированных клеток

Country Status (23)

Country Link
US (1) US7056723B2 (ru)
EP (1) EP1407006B9 (ru)
JP (2) JP5322252B2 (ru)
KR (1) KR100947976B1 (ru)
CN (1) CN1289663C (ru)
AT (1) ATE302268T1 (ru)
AU (1) AU2002352247B2 (ru)
BR (1) BRPI0215003B1 (ru)
CA (1) CA2467099C (ru)
DE (1) DE60205631T2 (ru)
DK (1) DK1407006T3 (ru)
EA (1) EA006485B1 (ru)
ES (1) ES2247404T3 (ru)
HK (1) HK1071765A1 (ru)
HU (1) HU227507B1 (ru)
IL (2) IL161526A0 (ru)
MX (1) MXPA04006001A (ru)
NO (1) NO335975B1 (ru)
NZ (1) NZ533586A (ru)
PL (1) PL205929B1 (ru)
SI (1) SI1407006T1 (ru)
UA (1) UA77735C2 (ru)
WO (1) WO2003054175A1 (ru)

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EE05680B1 (et) 2000-11-23 2013-10-15 Bavarian Nordic A/S Muundatud vaktsiiniaviirus, selle valmistamine ja kasutamine, seda sisaldav farmatseutiline kompositsioon ning vaktsiin
US7445924B2 (en) * 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
WO2004022729A1 (en) 2002-09-05 2004-03-18 Bavarian Nordic A/S Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
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US7625570B1 (en) * 2005-03-10 2009-12-01 The Regents Of The University Of California Methods for purifying adeno-associated virus
WO2008138533A1 (en) * 2007-05-14 2008-11-20 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
US8003363B2 (en) 2007-05-14 2011-08-23 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
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BRPI1007744A2 (pt) * 2009-05-12 2017-06-27 Transgene Sa método para a produção e purificação de um ortopoxvírus do tipo selvagem, ortopoxvírus do tipo selvagem, composição farmacêutica, uso de um ortopoxvírus do tipo selvagem e uso de uma linhagem de células aviárias imortalizadas
FR2953686B1 (fr) 2009-12-14 2012-11-02 Agronomique Inst Nat Rech Procede pour reduire la teneur bacterienne d'un milieu alimentaire et/ou biologique d'interet, contenant des gouttelettes lipidiques
CA2802430C (en) 2010-07-20 2021-07-20 Bavarian Nordic A/S Method for harvesting poxvirus
CN102353792A (zh) * 2011-07-07 2012-02-15 贵州大学 一种基于gpv p32蛋白的间接elisa试剂盒及制备方法
FR3042121A1 (fr) 2015-10-08 2017-04-14 Jean-Marc Limacher Composition anti-tumorale
EP3624851A1 (en) 2017-05-15 2020-03-25 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
WO2018211419A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
US20210187100A1 (en) 2018-09-06 2021-06-24 Bavarian Nordic A/S Storage Improved Poxvirus Compositions
KR20220153587A (ko) 2020-03-12 2022-11-18 버베리안 노딕 에이/에스 폭스바이러스 안정성을 개선하는 조성물
WO2024003238A1 (en) 2022-06-29 2024-01-04 Bavarian Nordic A/S Epstein-barr-virus vaccine
WO2024003239A1 (en) 2022-06-29 2024-01-04 Bavarian Nordic A/S RECOMBINANT MODIFIED saRNA (VRP) AND VACCINIA VIRUS ANKARA (MVA) PRIME-BOOST REGIMEN
CN115197966A (zh) * 2022-08-15 2022-10-18 深圳源兴基因技术有限公司 一种利用生物反应器大规模生产重组痘苗病毒载体的方法

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Also Published As

Publication number Publication date
EA006485B1 (ru) 2005-12-29
UA77735C2 (en) 2007-01-15
WO2003054175A1 (en) 2003-07-03
HU227507B1 (hu) 2011-07-28
EP1407006B1 (en) 2005-08-17
KR20040070263A (ko) 2004-08-06
CA2467099A1 (en) 2003-07-03
BR0215003A (pt) 2004-11-09
EP1407006A1 (en) 2004-04-14
DE60205631T2 (de) 2006-06-08
MXPA04006001A (es) 2005-08-19
SI1407006T1 (sl) 2006-04-30
PL369498A1 (en) 2005-04-18
US20040265986A1 (en) 2004-12-30
AU2002352247A1 (en) 2003-07-09
NO20043181L (no) 2004-07-19
DK1407006T3 (da) 2005-12-12
CA2467099C (en) 2013-01-29
JP2005512565A (ja) 2005-05-12
IL161526A (en) 2009-11-18
JP2010046093A (ja) 2010-03-04
HUP0402337A3 (en) 2010-01-28
BRPI0215003B1 (pt) 2017-03-14
US7056723B2 (en) 2006-06-06
NZ533586A (en) 2005-02-25
NO335975B1 (no) 2015-04-07
JP5322252B2 (ja) 2013-10-23
HK1071765A1 (en) 2005-07-29
ATE302268T1 (de) 2005-09-15
IL161526A0 (en) 2004-09-27
KR100947976B1 (ko) 2010-03-15
DE60205631D1 (de) 2005-09-22
AU2002352247B2 (en) 2008-08-07
ES2247404T3 (es) 2006-03-01
EP1407006B9 (en) 2006-10-18
PL205929B1 (pl) 2010-06-30
CN1606616A (zh) 2005-04-13
CN1289663C (zh) 2006-12-13
HUP0402337A2 (hu) 2005-01-28

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