ATE302268T1 - Methode zur gewinnung und reinigung von poxviren aus infizierten zellen - Google Patents

Methode zur gewinnung und reinigung von poxviren aus infizierten zellen

Info

Publication number
ATE302268T1
ATE302268T1 AT02787945T AT02787945T ATE302268T1 AT E302268 T1 ATE302268 T1 AT E302268T1 AT 02787945 T AT02787945 T AT 02787945T AT 02787945 T AT02787945 T AT 02787945T AT E302268 T1 ATE302268 T1 AT E302268T1
Authority
AT
Austria
Prior art keywords
infected cells
obtaining
virus
present
virus containing
Prior art date
Application number
AT02787945T
Other languages
English (en)
Inventor
Karl Heller
Jutta Kramer
Original Assignee
Bavarian Nordic As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bavarian Nordic As filed Critical Bavarian Nordic As
Application granted granted Critical
Publication of ATE302268T1 publication Critical patent/ATE302268T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Compounds Of Unknown Constitution (AREA)
AT02787945T 2001-12-20 2002-12-13 Methode zur gewinnung und reinigung von poxviren aus infizierten zellen ATE302268T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101928 2001-12-20
PCT/EP2002/014179 WO2003054175A1 (en) 2001-12-20 2002-12-13 Method for the recovery and purification of poxviruses from infected cells

Publications (1)

Publication Number Publication Date
ATE302268T1 true ATE302268T1 (de) 2005-09-15

Family

ID=8160920

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02787945T ATE302268T1 (de) 2001-12-20 2002-12-13 Methode zur gewinnung und reinigung von poxviren aus infizierten zellen

Country Status (22)

Country Link
US (1) US7056723B2 (de)
EP (1) EP1407006B9 (de)
JP (2) JP5322252B2 (de)
KR (1) KR100947976B1 (de)
CN (1) CN1289663C (de)
AT (1) ATE302268T1 (de)
AU (1) AU2002352247B2 (de)
BR (1) BRPI0215003B1 (de)
CA (1) CA2467099C (de)
DE (1) DE60205631T2 (de)
DK (1) DK1407006T3 (de)
EA (1) EA006485B1 (de)
ES (1) ES2247404T3 (de)
HU (1) HU227507B1 (de)
IL (2) IL161526A0 (de)
MX (1) MXPA04006001A (de)
NO (1) NO335975B1 (de)
NZ (1) NZ533586A (de)
PL (1) PL205929B1 (de)
SI (1) SI1407006T1 (de)
UA (1) UA77735C2 (de)
WO (1) WO2003054175A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7445924B2 (en) 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
HU230198B1 (hu) * 2000-11-23 2015-10-28 Bavarian Nordic A/S Módosított Vacciniavírus Ankara-variáns
WO2004022729A1 (en) 2002-09-05 2004-03-18 Bavarian Nordic A/S Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
WO2006052826A2 (en) * 2004-11-05 2006-05-18 The Government Of The United States Of America As Represented By The Secretary Methods for preparing cells and viruses
US7625570B1 (en) * 2005-03-10 2009-12-01 The Regents Of The University Of California Methods for purifying adeno-associated virus
JP2010526546A (ja) * 2007-05-14 2010-08-05 バヴァリアン・ノルディック・アクティーゼルスカブ ワクシニアウイルス系及び組換えワクシニアウイルス系ワクチンの精製
US8003363B2 (en) 2007-05-14 2011-08-23 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
CA2713891A1 (en) 2008-02-12 2009-08-20 Sanofi Pasteur Limited Methods using ion exchange and gel filtration chromatography for poxvirus purification
RU2011148791A (ru) * 2009-05-12 2013-06-20 Трансген Са Иммортализованные линии клеток птиц и их применение
FR2953686B1 (fr) 2009-12-14 2012-11-02 Agronomique Inst Nat Rech Procede pour reduire la teneur bacterienne d'un milieu alimentaire et/ou biologique d'interet, contenant des gouttelettes lipidiques
CA2802430C (en) 2010-07-20 2021-07-20 Bavarian Nordic A/S Method for harvesting poxvirus
CN102353792A (zh) * 2011-07-07 2012-02-15 贵州大学 一种基于gpv p32蛋白的间接elisa试剂盒及制备方法
FR3042121A1 (fr) 2015-10-08 2017-04-14 Jean-Marc Limacher Composition anti-tumorale
WO2018211419A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
US11306292B2 (en) 2017-05-15 2022-04-19 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
AU2019336940B2 (en) 2018-09-06 2025-09-11 Bavarian Nordic A/S Storage improved poxvirus compositions
JP7712284B2 (ja) 2020-03-12 2025-07-23 バヴァリアン・ノルディック・アクティーゼルスカブ ポックスウイルスの安定性を改善する組成物
AU2023298083A1 (en) 2022-06-29 2025-01-09 Bavarian Nordic A/S RECOMBINANT MODIFIED saRNA (VRP) AND VACCINIA VIRUS ANKARA (MVA) PRIME-BOOST REGIMEN
JP2025524459A (ja) 2022-06-29 2025-07-30 バヴァリアン・ノルディック・アクティーゼルスカブ エプスタイン・バー・ウイルスワクチン
CN115197966A (zh) * 2022-08-15 2022-10-18 深圳源兴基因技术有限公司 一种利用生物反应器大规模生产重组痘苗病毒载体的方法
FR3160186A1 (fr) 2024-03-13 2025-09-19 Odimma Therapeutics Vecteur non-viral pour transcription augmentee

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB877898A (en) 1956-12-06 1961-09-20 Apv Co Ltd A new or improved method of liberating enzymes
JPS5328989B2 (de) * 1974-05-27 1978-08-17
CH621573A5 (en) 1976-08-05 1981-02-13 Vnii Biosinteza Belkovykh Vesc Process for the continuous disintegration of microorganism cells and system for carrying out this process
JPS54154594A (en) * 1978-05-25 1979-12-05 Sumitomo Chem Co Ltd Extraction of glucose isomerase
WO1991001367A1 (en) * 1989-07-20 1991-02-07 Bioeng, Inc. Supercritical fluid disruption of and extraction from microbial cells
UA68327C2 (en) * 1995-07-04 2004-08-16 Gsf Forschungszentrum Fur Unwe A recombinant mva virus, an isolated eukaryotic cell, infected with recombinant mva virus, a method for production in vitro of polypeptides with use of said cell, a method for production in vitro of virus parts (variants), vaccine containing the recombinant mva virus, a method for immunization of animals
TW426733B (en) 1995-10-06 2001-03-21 Merck & Co Inc Method of disrupting cultured cells using an impinging jet device
MY150893A (en) * 1996-09-24 2014-03-14 Bavarian Nordic As Recombinant mva virus expressing dengue virus antigens, and the use thereof in vaccines
US5721120A (en) * 1996-10-02 1998-02-24 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
NZ299720A (en) * 1996-11-08 1999-07-29 Howick Engineering Ltd Epicyclic input/output annuli of same pcd, with planetary input/output gear sets, and with only one set having aligned teeth
JP4492826B2 (ja) * 1996-11-20 2010-06-30 イントロジェン セラピューティクス，インコーポレイテッド アデノウイルスベクターの産生および精製のための改良された方法
US6000551A (en) * 1996-12-20 1999-12-14 Eastman Chemical Company Method for rupturing microalgae cells
FR2787465A1 (fr) * 1998-12-21 2000-06-23 Transgene Sa Procede d'inactivation des virus enveloppes dans une preparation virale de virus non enveloppes
DE60029195T2 (de) * 1999-02-22 2007-06-28 Transgene S.A. Verfahren zur Gewinnung von purifizierter Virenzusammensetzung
DE19918619A1 (de) 1999-04-23 2000-10-26 Rhein Biotech Proz & Prod Gmbh Verfahren zum Gewinnen von rekombinantem HBsAg
US6951752B2 (en) * 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen

Also Published As

Publication number Publication date
EP1407006B9 (de) 2006-10-18
HUP0402337A3 (en) 2010-01-28
AU2002352247B2 (en) 2008-08-07
DE60205631T2 (de) 2006-06-08
EA200400833A1 (ru) 2004-12-30
HUP0402337A2 (hu) 2005-01-28
KR100947976B1 (ko) 2010-03-15
SI1407006T1 (sl) 2006-04-30
EP1407006A1 (de) 2004-04-14
UA77735C2 (en) 2007-01-15
CN1606616A (zh) 2005-04-13
AU2002352247A1 (en) 2003-07-09
PL205929B1 (pl) 2010-06-30
KR20040070263A (ko) 2004-08-06
DK1407006T3 (da) 2005-12-12
HU227507B1 (hu) 2011-07-28
HK1071765A1 (en) 2005-07-29
CN1289663C (zh) 2006-12-13
NO335975B1 (no) 2015-04-07
BR0215003A (pt) 2004-11-09
ES2247404T3 (es) 2006-03-01
JP2010046093A (ja) 2010-03-04
CA2467099A1 (en) 2003-07-03
EA006485B1 (ru) 2005-12-29
IL161526A0 (en) 2004-09-27
EP1407006B1 (de) 2005-08-17
NO20043181L (no) 2004-07-19
US20040265986A1 (en) 2004-12-30
US7056723B2 (en) 2006-06-06
DE60205631D1 (de) 2005-09-22
BRPI0215003B1 (pt) 2017-03-14
WO2003054175A1 (en) 2003-07-03
NZ533586A (en) 2005-02-25
PL369498A1 (en) 2005-04-18
JP2005512565A (ja) 2005-05-12
IL161526A (en) 2009-11-18
CA2467099C (en) 2013-01-29
JP5322252B2 (ja) 2013-10-23
MXPA04006001A (es) 2005-08-19

Similar Documents

Publication Publication Date Title
ATE302268T1 (de) Methode zur gewinnung und reinigung von poxviren aus infizierten zellen
DK1780269T3 (da) Virusrensningsmetoder
DE602004021192D1 (de) Verbesserte bisulfitkonversion von dna
SG156673A1 (en) Improved viral purification methods
ATE460473T1 (de) Immortalisierte entezelllinien zur viruserzeugung
DK1332209T3 (da) Samlinger af repeatproteiner indeholdende repeatmoduler
WO2001064835A8 (en) Novel nucleic acids and polypeptides
NO20075491L (no) Fremgangsmate for a behandle infeksjonssykdom-forverret astma
ATE411974T1 (de) Verfahren zur gewinnung von gereinigtem hydroxytyrosol aus von dem olivenbaum erhaltenen produkten und nebenprodukten
DK2261375T3 (da) Fremgangsmåde til hindring af viruscellefusion ved hæmning af funktionen af fusionsinitieringsområdet i RNA-vira med klasse I membranfusogene kappeproteiner
ATE430195T1 (de) Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle
WO2006091610A3 (en) Inhibitors of enveloped virus infectivity
ATE494271T1 (de) Verfahren zur reinigung und aufkonzentrierung von distickstoffmonoxid
DK1536015T3 (da) Promotorer til ekspression i modificeret vacciniavirus Ankara
ATE313641T1 (de) Expression von genen im modifizierten vaccinia virus ankara durch verwendung eines cowpox ati promoter
DK1534404T3 (da) Anvendelse af en klatratmodifikator til at fremme passagen af proteiner under nanofiltrering
DE602004015418D1 (de) Rekombinantes mva und verfahren zur erzeugung davon
ATE491952T1 (de) Screening auf inhibitoren des eindringens von hepatitis-c-virus
DE60231970D1 (de) Neurovirulenter stamm des west-nile-virus und anwendungen davon
DE50312368D1 (de) Rekombinante mva-stamme als potentielle impfstoffe gegen p. falciparum -malaria
ATE510933T1 (de) Zellen zum nachweis von enteroviren
ATE455863T1 (de) Methoden und zusammensetzungen zur identifikation von gen-funktion
WO2012082723A3 (en) Purification of immunogens using a non-polysaccharide matrix
EP0979874A3 (de) Protein und DNA-Sequenz der Pinosylvin-3-0-Methyltransferase (PMT)
Benkeg et al. Application of cyclic voltammetry in the study of platinum electrodigestion

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1407006

Country of ref document: EP