HUE033334T2 - Glikozid-hidroláz-61 családba tartozó fehérjék alkalmazása cellulóz feldolgozásában - Google Patents

Glikozid-hidroláz-61 családba tartozó fehérjék alkalmazása cellulóz feldolgozásában Download PDF

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Publication number
HUE033334T2
HUE033334T2 HUE11818904A HUE11818904A HUE033334T2 HU E033334 T2 HUE033334 T2 HU E033334T2 HU E11818904 A HUE11818904 A HU E11818904A HU E11818904 A HUE11818904 A HU E11818904A HU E033334 T2 HUE033334 T2 HU E033334T2
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ala
ser
thr
pro
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HUE11818904A
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Onorato Campopiano
Janos Torok
Dipnath Baidyaroy
Louis Clark
Kripa Rao
Lorand Szabo
Jie Yang
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Codexis Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (5)

  1. S^badslník IglKygóíB&k aksgkosablafe módMéidálHsob í.dié! MkrQk gmely a.ÜQ I!>:NC:2 aimml -$e$kY$&$f£v3$ W0-M SEQ ΙΟ; NCMi asedad szekvaíada GH6 ! akbykáaá IhiggMaaaaiiim! fegalább -mOMS a zekvanc ao lana!ma%:es :|) cebisíáa eozaosfees, kOxtük íegalább egy etulo^ítenM:- j(pGJ, 'Isgslább egy fi-glukoztáaai Qgalább bgy i. tspusá eeibidobidrolází (CBH i) és fcgaiábbegyQ. ίψΐίαύ eeifeblohidralázt (OBH2). %: Áz l, Í0uypv8t$wrhni kn^gníém·, ahol a GB6! íiíkéQg:: &} a gbjkóa ho¿&i»&! egy olyaa aeakekíbsa, msiybeK a: seddlBaiBG, :B^M^:OBIÍ:i :Ss:;CBH2:gfiaímek:í3r~ istmazó enzíttüco;nbmáci& cukfysitja eS; tegft&bb 20%-kaí «8?«$' ügpndyetg GlíSid iehérje jaQaíéQnsQkdli vé|l>esKsn6 re&kcióboz képesk b) a SEQ ID NOS szennn {.sekvenciávat. vggy: GikbSí akbvkásíé ddfQbab, yagy §w»~bm mmm 3¡t»t»iB!8a.v^«k.«ffiRe:M tabsins&syagy c) radsimazza a SEQ li> NO:2 szerinii sxekyenbitó vagy.amsHMb? stóe&áiis¡'i
  2. 3. Az í!s..#á¡|$2, tg&amp;yp&amp;ti szsdíki készsbaéay, aboba eelMfe eazimsk:: a í ívk un;birtíbisak, b) gomfeaeozsroek é:kva.gy &amp;I¡M íhmmphiíából mkrmmsik. 4, A» i~X igéaypaaíbk bánselvíke szeriRi! késxkméísy, ahoí a ksszlbíiddy a kbvetkes&amp;k hámsebdke izenm van eQálítys;: &amp;} a QEbSI iféhérjét. kAdbid gé» eKpresszálása egy gazdasejkgsQpaiMbbaa éa bgaiábS^ agy, «ellaiáz en-zbdbtkbdeib géa expmsszáMsa egy mtókpzdaaQt-pi^aiaesébaa, vagy b| a Qllbl fehárQt kódolb gé« bs legalsbb egy, celíidáa eazíiKsiikíkkdé gáa expi-esszá!ása agpttábbañ a gaadas<|«!p«sban. Si. á 4. ígényganí: aaariasí MsKítaiiIsyy áhol a géa bsismlóg sziiaaígagtidat tai-ialmazA !®b<S¡pí kódol. b. i|Ms ItosntÉhaié oifeík aíAállitáaÉra ceíkaóabQamsszSbÁi;, smeiy eiiáfás ianataa» .CSMiSai íbMdét ¿s celhiM eazlmaket ianateazA Ms*telnny«li»."k»y^^· aeggeí vagy pjsbzáUsamtak ahoi: a) a GHili fbygQ aMQdD b?Ü:2 szsghti azakvsacíáyal vagy bl0l&amp;ftóÉ®;:#te ailb 80%-ban a/ooog%mkom^mkvm&amp;ki datafmaziáx by a caikd&amp;K e¡mmek tarlabnazaak kgatóbb ®gy ejiiogtókanázt igpíáfeb egy f-gíük«)z®áat |BiGLg kgaláb!> agy I. ueÜobiohídroBzi (OBI U ! és fegaíább agy 2, iipusú cdbbiohkboiási (OBI 12). 7. A 6. igéaygoat .:na-¡iati aljiirás, abo!; a|&amp;í á:'iígk<-.;.-:U-:Ka; etóu a a;«alss>dríd: Qdkaassivo vafg b) t; késáSÍíodojy teíiyészíbkdzeg vagy so|ÍÍizab¡aí bakal^ag; k>ykbfed ggy :V8gy:-: íAbb, m. a¡oiilfe<;ík; gbskoaíydlgzok: í) iCU <;} a ceHüíóxssiíbsKSTát bú&amp;áhók bd;?as«bmábék cirokbá!, kak«r!c»ból, rissbbl árpábók cukomádbók Mpatók eutoñádbb!, «íakbbk vesszbs kblesbbk kukoncaszasbbk kakodeacimtkábói, kukoñcaroahó* vagy es;k luímbbukibibóí s?.ántt;»;iL &amp;M a sziú>syUñ.l adotí «seibeft hókasxatadbbi sfekmaask, és/vsgy d) &amp; fennesísahaté ('itk&amp;r íamlmas gjakózk vagy tv 2gdpyp(»b variad: «bébla, ahal &amp; késátaány, íeaydsx^kdzgg; tarblmak iasaÉIk <QB6i. fehdgiéí da Isgslílbb egyeí vagy tdbbek a kbvsikeadk kbaiMk: isRdák eadpgtSksafe CHS}., ísofáU |b gi0kí>pídáai (BGL), smBb í, bpasü csHabfe&amp;íámMxt C£2W!J és/vagy ksdab 3. , dpasb celtobsofbdrsM»: CCBB2).
  3. 9, BÍj&amp;ás vdgíerméfc elbáilkassm cekaifezabsiBrádsdí, amely djáirás tiartalmawía a kbvsíkezákdí:: ;a). a ceitóíéSsssmbzfték M, jgénjpmísk:': $$rmeiy&amp;@ saatínti kdszkméRaysk eaálísl a szuhszifásbbl ifermemálfeaíá eukrok elMkb&amp;síg ds b) a fenemálbatb eakmfe ddoíkaxkíidss fermestádiás :s;pj'ásbas mikswpaiímo®s&amp;l5 vaáteb: vbgípr·-mák clbsillftása. 19, ,ñ 9. i^hypmi-ssmánii apráSj.-am^: stómstfei. dfedtosMS #: feaiesdálife aljásás,. II. &amp;Sk isdayipaiszariabdljMs,abolaideliulbKSKubssi^í'eilcgiífoaítása és afemeaiá® egymásí kd-vende» íbrtórdk.
  4. 12,, Eli;kás::Vdg?8rmdk;®:IMllkásárs eaifalézszabszpkdbák amdy «prás tartaísnazza a Mveikezbkei; a) b"E, Igpypaatek bármdyike síljai^üísaí: «Idákkftttfkna^taíbaté ewkrokeíMlSMss, es b) a femeaíáihaíb eukmk étísíkex^üém ^rmeatádíbs eyirásbaa mikmorganimuss&amp;k «záikd: vegle*-mek «ibáilltása. O, Λ 9~12, igédypeítfok háímdyske sxerínti epeSa, ahal a x?%lerméfe aíkobol.
  5. 14, Á Π, igénypomszemií ellarás, ahoi sx askeW slaaol, U, A: 9*15. igényponísdv bármdyske szengíi epeás* ahaí a míkmmmtésmm ék¡m&amp;.
HUE11818904A 2010-08-20 2011-08-22 Glikozid-hidroláz-61 családba tartozó fehérjék alkalmazása cellulóz feldolgozásában HUE033334T2 (hu)

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US (2) US9493802B2 (hu)
EP (1) EP2606131B1 (hu)
CN (1) CN103080306B (hu)
BR (1) BR112013003906A2 (hu)
CA (1) CA2807702C (hu)
DK (1) DK2606131T3 (hu)
ES (1) ES2623288T3 (hu)
HU (1) HUE033334T2 (hu)
WO (1) WO2012024698A1 (hu)

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* Cited by examiner, † Cited by third party
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US8993286B2 (en) * 2011-05-19 2015-03-31 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
BR112013033614A2 (pt) 2011-06-30 2018-05-29 Codexis Inc fermentação de pentose por microorganismo recombinante
DK2748317T3 (en) 2011-08-22 2017-07-17 Codexis Inc GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
WO2013039776A1 (en) 2011-09-13 2013-03-21 Novozymes North America, Inc. Methods of hydrolyzing and fermenting cellulosic material
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BR112015005884A2 (pt) * 2012-09-19 2017-08-08 Novozymes Inc E Novozymes As processos para degradação de um material celulósico, para produção de um produto da fermentação, e de fermentação de um material celulósico, composição, e, formulação de caldo inteiro ou composição de cultura de células
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CN103275956B (zh) * 2013-05-26 2014-12-10 山东隆科特酶制剂有限公司 一种具有木质素降解增效作用的纤维素酶CelH61及其基因和应用
CN103320415B (zh) * 2013-06-02 2014-11-26 武汉新华扬生物股份有限公司 一种耐热中性纤维素酶Cel61及其基因和应用
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WO2015042543A2 (en) * 2013-09-20 2015-03-26 The Trustees Of Columbia University In The City Of New York Biofuel production enzymes and uses thereof
DK3063285T3 (da) * 2013-11-01 2019-05-13 Novozymes As Fremgangsmåder til forsukring og fermentering af et celluloseholdigt materiale
ES2542621B1 (es) * 2014-02-07 2016-08-16 Abengoa Bioenergía Nuevas Tecnologías, S.A. Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
JP6364662B2 (ja) * 2014-09-17 2018-08-01 本田技研工業株式会社 ＧＨファミリー３に属する耐熱性β―キシロシダーゼ
CN104498456B (zh) * 2014-12-01 2017-03-29 中国农业科学院饲料研究所 一种酸性β‑葡萄糖苷酶Bgl3B及其基因和应用
CN106148208B (zh) * 2015-03-23 2020-12-18 中国科学院天津工业生物技术研究所 新的二元有机酸生产菌株及其制备和应用
CN106148209B (zh) * 2015-03-23 2020-11-20 中国科学院天津工业生物技术研究所 新的二元有机酸生产菌株及其制备和应用
CN105039287B (zh) * 2015-05-21 2018-06-19 中国科学院天津工业生物技术研究所 GH61家族糖苷水解酶基因PpGH61及其用途
US10626381B2 (en) * 2015-06-23 2020-04-21 Abengoa Bioenergía Nuevas Tecnologías, S. A. Cellulolytic compositions comprising monooxygenase polysaccharide enzymes with improved activity
CN105949735B (zh) * 2016-06-14 2018-05-25 江门市扬帆实业有限公司 一种性能优异的改性木质素聚乳酸塑料
CN109957571A (zh) * 2017-12-14 2019-07-02 中国科学院大连化学物理研究所 一种多糖裂解单加氧酶编码基因及酶和制备与应用
CN109929855B (zh) * 2017-12-15 2022-12-13 中国科学院大连化学物理研究所 多糖裂解单加氧酶lpmo 9d编码基因及酶与制备和应用
CN108796026A (zh) * 2018-06-29 2018-11-13 齐齐哈尔龙江阜丰生物科技有限公司 赖氨酸的生产、提取以及纯化工艺
CN111850004B (zh) * 2020-07-27 2022-04-22 齐鲁工业大学 一种活性提高的纤维小体对接蛋白突变体36740及应用
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CN114107257B (zh) * 2021-11-24 2023-08-08 广东省科学院微生物研究所(广东省微生物分析检测中心) 一种糖苷水解酶及其应用
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