HUE026473T2 - Oldható, csak nehézláncot tartalmazó ellenanyag - Google Patents
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- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
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- C—CHEMISTRY; METALLURGY
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Claims (7)
- Szabadabtil Igénypontok L lejárás cs^ antigéidiez speőii|pap köbfeő ellenanyag előállítására, amely hatalmazza a köveik öKÖkseí: (a) embertől külőnböaö, transigatifeas emlők immutúzáiása .az antigénnek afeöl az emlős ess& nehézláoeöt tartalmazó, a posztranszkripeios és feldolgozott aefe^í^^E^^iÉia-.CHl-áöajé!^ nem tartalmazó ellenanyagot expresszál, és ahol a csak nebéziáncöt tartalma# eliosanysgm emlősben;: jelesiává irasKzpmkfs lók úszni; expresszálódik; (b) hosszú élettl plazmasejt vagy memória P-ssjl izolálása az intmtmizá|i: emlősből; fel mRNS-popnlácIó izolálása a (ti) lépésben Izolált sejtből; (d) a (c) lépésben izolált mRIN'S-ből sÄoaztateti eDNS-popnlaeíő klónozása expressziős vektorba és az expressziós vektor ékpesszátása sejfeooaibím: és (<;) legalább egy sejívonal kíválh$zt|sm amely az· asiísgéöhez specifikusan köiodo, csalt nehézláncot ía«akíazéi«lÍeR:aayagot::íbntíe3, ahol az eoöös egérvagy patkány.
- 2: Rprás oldható, astígénhex specifikusan kőtődő f^smlfis vágj? % Ifelss fehérje előállítására, nmei y tattalmszza h kővetkezőket: |á$ jémSertőf MSRmbőző, írímszgeniktis: emlős :1ΜΛΛ» az miipbnei «tel az eoslös csak nelfezliaeot tartalmaző, aposzirarsszkripeiös és feldolgozóit ssbéziásemsRfeS-ben CMI-doofepï: geo? mrtahnasó ellenanyagot expresszál és ahol a ssak nébézááseot tartalmazó ellenanyag az emlősben jelenlévő banszgeolkos Mkoszrel: expmsszálMfc vagy memória 8-sejt (e) raRbíS-populádo íznláfesa a fb> lépésben kapott sejtből; (d) a (e) lépésben Izolált mRNS-hől szarfeazó VVőmnént kódoló cDMS-i tartalmazó eDNS-popeláclö klónozása expressziós vektorba oly módon, hogy a Vn-domén vagy VK lúzíos fehérje, amely tartalipazbat dehézllne effektor téglát,yp^resszálódik a sejívxonrifean; és (e) legalább egy, az amigéphsz speeiikpsaB kötődő bkj-dorném vagy VM fúziós fehérjéi termelő seRvenal kiválasztása, ahol az emlős egér vagy patkány.
- 3. Az 1. vagy 2. igénypont szerinti eljárás, alsói az antigénnel provokált, embertől kökmböző, p^szpsaktísmhlös egér, és a hosszú életű plazmaseji vagy memória B-sejí egér másodlagos nyirokmirígyéböí, csontvelőjéből vagy lépőből van tisztítva.
- 4. Az előző igénypontok bátotelyike szedői «|áA, aboi o hossza életű pSaztoasejt memória S^· sè^:«,s^ÂaR:iSvô:a^Â^&ï ioarkeíokfeihssztíálásávaí van tisztítva.
- 5. A 4. igénypont szerinti eljárás, ahol az aatigésmel provokált, embertől különböző, transzgenikus emlős egér és a sejtfelszíni marker a CI>138 market.
- 6. Az 1-5. igénypontok bármelyike szerinti eigápás, ahol a sejtvona! nükrobsálls sejtvonal vagy endösseitvooal
- 7. Az 1-6. igénypontok bármelyike szerinti eljárás, ahol a (d) lépes tartalmazza a kővetkezőt: az expressziós vektor tnniszfektálása baktérinmba, nagy átmenő t&ljesítméeyflö, automatizálható formátumban, a hskiéttma .ámplífikáíása, as iekp&SSÂÎÔs vektor izolálása, és az exfaessziós vektor trasszfefctálása: ezpéssziás sejtbe. S. Az l~7. igesypoatök bármelyike szedői: e|árásS: ahol az í®M% Nesszé «tel |lráitae$gl& i«!8gj$t zpspfeftes tmamrmlisälvs vaanak. 9; A §. igónypőat szerinti eljárás, shd a sejtek reielőmasejttel történő fúzióval vagy virassal, példa«! EBV-ve! történő traaszformác sóval vannak: Immortal ízáiva. iö. Az Î -9. igénypontok bármelyike szerinti eljárás, ahol bármely, csak aebezíátteei tartalmazó ellenanysg-lókasz magába« foglal legalább egy dommátts szek-kt.lv markergént. t|. AzÄ ipttptmttit bármélgike szerinti e|ii^pndy taïîâpaam továbbá a kővetkező fépéstí m okiható Wdomén vagy az oldható Védőméit!: kódoló nukieinsav izolálása a kívánt amjpp-spedllcltásá. csak nótóglitteoí táta# eiláóáayagoí vagy % íiziós Mtétjéí termelő B~se$tből vagy imtöortaltzáit sejtvooalbök 52. Az Hí . igénypontok bármelyike szerinti eljárás, amely tartalmazza továbbá a kővetkező lépést: az oldható VH-domén vagy az oldható Virdoment kódoló mikiemsav Izolálása az emlős által termeit, csak nehéziárteot tartalmazó ellenanyagot tömeló: sejtből vagy az emíésiiltát ^Â;lt,'ti8tRô®ÉllâS8lt,..^ltiÂé^^eot vagy Vj) tozíós feherjat ienóelő sejtbok óiaplay-eljárással.
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| HUE10711069A HUE026473T2 (hu) | 2009-03-24 | 2010-03-19 | Oldható, csak nehézláncot tartalmazó ellenanyag |
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| JP (1) | JP5836927B2 (hu) |
| KR (1) | KR20120068757A (hu) |
| CN (1) | CN102482342B (hu) |
| BR (1) | BRPI1012694A2 (hu) |
| CA (1) | CA2756171C (hu) |
| CY (1) | CY1117203T1 (hu) |
| DK (1) | DK2411408T4 (hu) |
| ES (1) | ES2563321T5 (hu) |
| FI (1) | FI2411408T4 (hu) |
| GB (1) | GB0905023D0 (hu) |
| HR (1) | HRP20160158T4 (hu) |
| HU (1) | HUE026473T2 (hu) |
| MX (1) | MX2011010027A (hu) |
| PL (1) | PL2411408T5 (hu) |
| PT (1) | PT2411408E (hu) |
| RU (1) | RU2528737C2 (hu) |
| SG (1) | SG174510A1 (hu) |
| SI (1) | SI2411408T2 (hu) |
| SM (1) | SMT201600044B (hu) |
| TW (1) | TW201040264A (hu) |
| WO (1) | WO2010109165A2 (hu) |
| ZA (1) | ZA201106983B (hu) |
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| MX2007000921A (es) | 2004-07-22 | 2007-11-09 | Univ Erasmus Medical Ct | Moleculas de union. |
| CA2638117A1 (en) * | 2006-01-25 | 2007-08-30 | Erasmus University Medical Center Rotterdam | Generation of heavy-chain only antibodies in transgenic animals |
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