HRP20100640T1 - Postupak proizvodnje rekombinantnog poliklonskog proteina - Google Patents
Postupak proizvodnje rekombinantnog poliklonskog proteina Download PDFInfo
- Publication number
- HRP20100640T1 HRP20100640T1 HR20100640T HRP20100640T HRP20100640T1 HR P20100640 T1 HRP20100640 T1 HR P20100640T1 HR 20100640 T HR20100640 T HR 20100640T HR P20100640 T HRP20100640 T HR P20100640T HR P20100640 T1 HRP20100640 T1 HR P20100640T1
- Authority
- HR
- Croatia
- Prior art keywords
- cells
- polyclonal
- protein
- cell line
- expression
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract 30
- 102000004169 proteins and genes Human genes 0.000 title claims abstract 29
- 238000000034 method Methods 0.000 title claims abstract 20
- 238000004519 manufacturing process Methods 0.000 title claims abstract 6
- 150000007523 nucleic acids Chemical group 0.000 claims abstract 5
- 238000001890 transfection Methods 0.000 claims abstract 3
- 239000013598 vector Substances 0.000 claims abstract 2
- 210000004027 cell Anatomy 0.000 claims 43
- 239000013604 expression vector Substances 0.000 claims 11
- 239000003550 marker Substances 0.000 claims 7
- 229920001184 polypeptide Polymers 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 210000004962 mammalian cell Anatomy 0.000 claims 3
- 108091023040 Transcription factor Proteins 0.000 claims 2
- 102000040945 Transcription factor Human genes 0.000 claims 2
- 210000000349 chromosome Anatomy 0.000 claims 2
- 210000003527 eukaryotic cell Anatomy 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 230000010354 integration Effects 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108091026890 Coding region Proteins 0.000 claims 1
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 206010035226 Plasma cell myeloma Diseases 0.000 claims 1
- 230000010261 cell growth Effects 0.000 claims 1
- 238000010367 cloning Methods 0.000 claims 1
- 210000002950 fibroblast Anatomy 0.000 claims 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims 1
- 230000012010 growth Effects 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 201000000050 myeloid neoplasm Diseases 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 230000035899 viability Effects 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 239000012228 culture supernatant Substances 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Non-Volatile Memory (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200700764 | 2007-05-25 | ||
| US92470807P | 2007-05-29 | 2007-05-29 | |
| DKPA200701292 | 2007-09-07 | ||
| US96000207P | 2007-09-11 | 2007-09-11 | |
| PCT/DK2008/050116 WO2008145133A2 (en) | 2007-05-25 | 2008-05-21 | Method for manufacturing a recombinant polyclonal protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20100640T1 true HRP20100640T1 (hr) | 2010-12-31 |
Family
ID=39739965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HR20100640T HRP20100640T1 (hr) | 2007-05-25 | 2010-11-24 | Postupak proizvodnje rekombinantnog poliklonskog proteina |
Country Status (23)
| Country | Link |
|---|---|
| US (1) | US7910332B2 (ja) |
| EP (1) | EP2152872B1 (ja) |
| JP (2) | JP5941616B2 (ja) |
| KR (1) | KR101555658B1 (ja) |
| CN (1) | CN101688204B (ja) |
| AT (1) | ATE481483T1 (ja) |
| AU (1) | AU2008255383B2 (ja) |
| BR (1) | BRPI0812091A2 (ja) |
| CA (1) | CA2683800C (ja) |
| CY (1) | CY1110939T1 (ja) |
| DE (1) | DE602008002593D1 (ja) |
| DK (1) | DK2152872T3 (ja) |
| HK (1) | HK1134519A1 (ja) |
| HR (1) | HRP20100640T1 (ja) |
| IL (1) | IL201395A (ja) |
| MX (1) | MX2009012526A (ja) |
| NZ (1) | NZ581316A (ja) |
| PL (1) | PL2152872T3 (ja) |
| PT (1) | PT2152872E (ja) |
| SI (1) | SI2152872T1 (ja) |
| TW (1) | TW200902548A (ja) |
| WO (1) | WO2008145133A2 (ja) |
| ZA (1) | ZA200908021B (ja) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EA013225B1 (ru) | 2003-01-07 | 2010-04-30 | Симфоген А/С | Способ получения линии клеток, продуцирующей рекомбинантный поликлональный белок, способ получения поликлонального белка, линия клеток, продуцирующая рекомбинантный поликлональный белок, библиотека векторов, популяция клеток |
| EP2121920B1 (en) * | 2007-03-01 | 2011-08-24 | Symphogen A/S | Method for cloning cognate antibodies |
| DE602008002593D1 (de) * | 2007-05-25 | 2010-10-28 | Symphogen As | Verfahren zur herstellung eines rekombinanten polyklonalen proteins |
| WO2009030237A2 (en) * | 2007-09-07 | 2009-03-12 | Symphogen A/S | Methods for recombinant manufacturing of anti-rsv antibodies |
| CN103525751B (zh) * | 2008-02-01 | 2017-04-12 | 克罗莫塞尔公司 | 细胞系以及制备和使用其的方法 |
| EP2098536A1 (en) | 2008-03-05 | 2009-09-09 | 4-Antibody AG | Isolation and identification of antigen- or ligand-specific binding proteins |
| RU2010147652A (ru) * | 2008-04-23 | 2012-05-27 | Симфоген А/С (Dk) | Способы производства поликлонального белка |
| WO2009155008A1 (en) * | 2008-05-28 | 2009-12-23 | Bayer Healthcare Llc | Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells |
| AU2009287165A1 (en) * | 2008-08-29 | 2010-03-04 | Symphogen A/S | Method for cloning avian-derived antibodies |
| ES2533952T3 (es) | 2009-02-09 | 2015-04-16 | Morphosys Ag | Producción de mezclas oligoclonales de inmunoglobulinas en células individuales |
| JP6033783B2 (ja) | 2010-11-01 | 2016-11-30 | シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ | Ｐａｎ−ｈｅｒ抗体組成物 |
| DK2640743T3 (en) * | 2010-11-16 | 2017-01-23 | Excelimmune Inc | PROCEDURES FOR THE PREPARATION OF RECOMBINANT PROTEINS |
| CN102558353A (zh) * | 2011-12-09 | 2012-07-11 | 上海应用技术学院 | 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法 |
| GB2502127A (en) | 2012-05-17 | 2013-11-20 | Kymab Ltd | Multivalent antibodies and in vivo methods for their production |
| AU2013255537B2 (en) | 2012-05-02 | 2018-02-15 | Symphogen A/S | Humanized pan-her antibody compositions |
| US9790269B2 (en) | 2013-02-08 | 2017-10-17 | Misfolding Diagnostics, Inc. | Transthyretin antibodies and uses thereof |
| ES2748295T3 (es) | 2014-09-16 | 2020-03-16 | Symphogen As | Anticuerpos anti-MET y composiciones |
| US20170044608A1 (en) * | 2015-07-17 | 2017-02-16 | Allele Biotechnology & Pharmaceuticals, Inc. | Methods of selecting antibodies and antibody fragments |
| EP3402886A1 (en) * | 2016-01-14 | 2018-11-21 | Anton Bauer | Method of producing a production cell line |
| WO2018073393A2 (en) | 2016-10-19 | 2018-04-26 | Cellectis | Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy |
| CN110621698B (zh) | 2017-04-05 | 2024-04-12 | 法国施维雅药厂 | 靶向pd-1、tim-3及lag-3的组合疗法 |
| CA3104390A1 (en) | 2018-06-22 | 2019-12-26 | Genmab A/S | Method for producing a controlled mixture of two or more different antibodies |
| CN109988751A (zh) * | 2019-01-15 | 2019-07-09 | 济宁学院 | 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法 |
| US20220243197A1 (en) * | 2019-04-30 | 2022-08-04 | Gigagen, Inc. | Recombinant Polyclonal Proteins and Methods of Use Thereof |
| CN114729309A (zh) * | 2019-09-27 | 2022-07-08 | 葛兰素史密斯克莱知识产权发展有限公司 | 预测克隆细胞系生产稳定性的方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0530216A4 (en) | 1990-04-19 | 1993-06-30 | Medimmune, Inc. | Administration of monoclonal antibodies against respiratory viruses |
| GB9022543D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Antibody production |
| US6111166A (en) | 1994-09-19 | 2000-08-29 | Medarex, Incorporated | Transgenic mice expressing human Fcα and β receptors |
| GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
| EP1293564A4 (en) * | 2000-06-08 | 2005-08-17 | Mitsubishi Pharma Corp | EXPRESSION VECTOR FOR SCREENING CELLS HIGH EXPRESSION OF RECOMBINANT PROTEINS, TRANSFORMING HIGH EXPRESSION OF A RECOMBINANT PROTEIN AND USE THEREOF |
| US6849259B2 (en) * | 2000-06-16 | 2005-02-01 | Symphogen A/S | Polyclonal antibody composition for treating allergy |
| CA2965865C (en) * | 2002-07-18 | 2021-10-19 | Merus N.V. | Recombinant production of mixtures of antibodies |
| WO2004029284A2 (en) | 2002-09-30 | 2004-04-08 | Protein Design Labs, Inc. | Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes |
| EA013225B1 (ru) * | 2003-01-07 | 2010-04-30 | Симфоген А/С | Способ получения линии клеток, продуцирующей рекомбинантный поликлональный белок, способ получения поликлонального белка, линия клеток, продуцирующая рекомбинантный поликлональный белок, библиотека векторов, популяция клеток |
| CN100383244C (zh) * | 2003-01-07 | 2008-04-23 | 西福根有限公司 | 用于生产重组多克隆蛋白质的方法 |
| US20060275766A1 (en) * | 2003-01-07 | 2006-12-07 | Haurum John S | Method for manufacturing recombinant polyclonal proteins |
| TWI333977B (en) * | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
| MX2007000644A (es) * | 2004-07-20 | 2007-03-28 | Symphogen As | Anticuerpo policlonal recombinante anti-rhesus d y metodos de fabricacion. |
| BRPI0513714A (pt) * | 2004-07-20 | 2008-05-13 | Symphogen As | procedimento para caracterização estrutural de uma proteìna policlonal recombinante ou uma linhagem de célula policlonal |
| JP2009518320A (ja) | 2005-12-05 | 2009-05-07 | シュムフォウエン アクティーゼルスカブ | 抗オルトポックスウイルス組換えポリクローナル抗体 |
| KR20080113223A (ko) | 2006-03-06 | 2008-12-29 | 심포젠 에이/에스 | 호흡기세포 융합 바이러스 감염 치료용 재조합 폴리클로날 항체 |
| DE602008002593D1 (de) * | 2007-05-25 | 2010-10-28 | Symphogen As | Verfahren zur herstellung eines rekombinanten polyklonalen proteins |
-
2008
- 2008-05-21 DE DE602008002593T patent/DE602008002593D1/de active Active
- 2008-05-21 PT PT08748815T patent/PT2152872E/pt unknown
- 2008-05-21 WO PCT/DK2008/050116 patent/WO2008145133A2/en active Application Filing
- 2008-05-21 EP EP08748815A patent/EP2152872B1/en not_active Not-in-force
- 2008-05-21 PL PL08748815T patent/PL2152872T3/pl unknown
- 2008-05-21 BR BRPI0812091-9A2A patent/BRPI0812091A2/pt not_active Application Discontinuation
- 2008-05-21 SI SI200830107T patent/SI2152872T1/sl unknown
- 2008-05-21 AU AU2008255383A patent/AU2008255383B2/en not_active Ceased
- 2008-05-21 KR KR1020097027038A patent/KR101555658B1/ko active IP Right Grant
- 2008-05-21 AT AT08748815T patent/ATE481483T1/de active
- 2008-05-21 DK DK08748815.1T patent/DK2152872T3/da active
- 2008-05-21 JP JP2010509679A patent/JP5941616B2/ja not_active Expired - Fee Related
- 2008-05-21 CA CA2683800A patent/CA2683800C/en not_active Expired - Fee Related
- 2008-05-21 CN CN2008800174383A patent/CN101688204B/zh not_active Expired - Fee Related
- 2008-05-21 MX MX2009012526A patent/MX2009012526A/es active IP Right Grant
- 2008-05-21 NZ NZ581316A patent/NZ581316A/en not_active IP Right Cessation
- 2008-05-23 TW TW097119118A patent/TW200902548A/zh unknown
- 2008-05-23 US US12/153,809 patent/US7910332B2/en not_active Expired - Fee Related
-
2009
- 2009-10-11 IL IL201395A patent/IL201395A/en active IP Right Grant
- 2009-11-13 ZA ZA2009/08021A patent/ZA200908021B/en unknown
-
2010
- 2010-03-09 HK HK10102454.3A patent/HK1134519A1/xx not_active IP Right Cessation
- 2010-11-24 CY CY20101101058T patent/CY1110939T1/el unknown
- 2010-11-24 HR HR20100640T patent/HRP20100640T1/hr unknown
-
2014
- 2014-09-19 JP JP2014191912A patent/JP2015037411A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| MX2009012526A (es) | 2009-12-09 |
| AU2008255383A1 (en) | 2008-12-04 |
| WO2008145133A3 (en) | 2009-02-05 |
| KR101555658B1 (ko) | 2015-09-24 |
| PL2152872T3 (pl) | 2011-03-31 |
| NZ581316A (en) | 2012-02-24 |
| CY1110939T1 (el) | 2015-06-10 |
| ZA200908021B (en) | 2010-11-24 |
| JP5941616B2 (ja) | 2016-06-29 |
| IL201395A (en) | 2015-09-24 |
| SI2152872T1 (sl) | 2011-01-31 |
| PT2152872E (pt) | 2010-11-17 |
| CA2683800C (en) | 2015-03-17 |
| ATE481483T1 (de) | 2010-10-15 |
| HK1134519A1 (en) | 2010-04-30 |
| EP2152872A2 (en) | 2010-02-17 |
| AU2008255383B2 (en) | 2012-11-01 |
| CA2683800A1 (en) | 2008-12-04 |
| CN101688204A (zh) | 2010-03-31 |
| DK2152872T3 (da) | 2010-12-06 |
| TW200902548A (en) | 2009-01-16 |
| BRPI0812091A2 (pt) | 2014-10-14 |
| JP2011504721A (ja) | 2011-02-17 |
| WO2008145133A2 (en) | 2008-12-04 |
| IL201395A0 (en) | 2011-08-01 |
| KR20100017923A (ko) | 2010-02-16 |
| JP2015037411A (ja) | 2015-02-26 |
| CN101688204B (zh) | 2013-07-03 |
| EP2152872B1 (en) | 2010-09-15 |
| US7910332B2 (en) | 2011-03-22 |
| US20090111142A1 (en) | 2009-04-30 |
| DE602008002593D1 (de) | 2010-10-28 |
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