GB2415432B - Islet cells from human embryonic stem cells - Google Patents

Islet cells from human embryonic stem cells

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Publication number
GB2415432B
GB2415432B GB0517624A GB0517624A GB2415432B GB 2415432 B GB2415432 B GB 2415432B GB 0517624 A GB0517624 A GB 0517624A GB 0517624 A GB0517624 A GB 0517624A GB 2415432 B GB2415432 B GB 2415432B
Authority
GB
United Kingdom
Prior art keywords
cells
embryonic stem
human embryonic
islet
stem cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
GB0517624A
Other languages
English (en)
Other versions
GB0517624D0 (en
GB2415432A (en
Inventor
Gregory J Fisk
Margaret S Inokuma
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Geron Corp
Original Assignee
Geron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23326555&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=GB2415432(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Geron Corp filed Critical Geron Corp
Publication of GB0517624D0 publication Critical patent/GB0517624D0/en
Publication of GB2415432A publication Critical patent/GB2415432A/en
Application granted granted Critical
Publication of GB2415432B publication Critical patent/GB2415432B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • A61K35/37Digestive system
    • A61K35/39Pancreas; Islets of Langerhans
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Families Citing this family (224)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6558952B1 (en) * 1992-12-14 2003-05-06 Waratah Pharmaceuticals, Inc. Treatment for diabetes
US20040037818A1 (en) * 1998-07-30 2004-02-26 Brand Stephen J. Treatment for diabetes
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US7514260B2 (en) * 2004-05-21 2009-04-07 Wicell Research Institute, Inc. Feeder independent extended culture of embryonic stem cells
US7473555B2 (en) * 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
CA2469209C (en) * 2001-12-04 2012-04-24 Jianjian Jin Cultured cells from pancreatic islets
KR101008868B1 (ko) * 2001-12-07 2011-01-17 제론 코포레이션 인간 배아 줄기세포 유래의 섬세포
JP4374469B2 (ja) * 2002-01-09 2009-12-02 独立行政法人科学技術振興機構 記憶障害治療剤
US20050090004A1 (en) * 2003-01-16 2005-04-28 Sayre Chauncey B. Stem cell maturation for all tissue lines
US20050170506A1 (en) * 2002-01-16 2005-08-04 Primegen Biotech Llc Therapeutic reprogramming, hybrid stem cells and maturation
US20030134422A1 (en) * 2002-01-16 2003-07-17 Sayre Chauncey Bigelow Stem cell maturation for all tissue lines
WO2003068357A2 (en) * 2002-02-12 2003-08-21 Cornell Research Foundation, Inc. Identification and high-yield isolation of human pancreatic islet progenitor and stem cells
EP2361966A1 (en) 2002-05-17 2011-08-31 Mount Sinai School of Medicine of New York University Mesoderm and definitive endoderm cell populations
US20060003446A1 (en) * 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
IL165242A0 (en) * 2002-05-24 2005-12-18 Waratah Pharmaceuticals Inc Treatment for diabetes
US20040002447A1 (en) * 2002-06-04 2004-01-01 Regents Of The University Of California Induction of insulin expression
PL376473A1 (en) * 2002-10-22 2005-12-27 Waratah Pharmaceuticals, Inc. Treatment of diabetes
SI1572984T1 (sl) 2002-12-16 2016-07-29 Technion Research & Development Foundation Ltd. Kulturni sistem brez hranilnih celic, brez kseno-komponent, za humane embrionalne matične celice
US7807458B2 (en) * 2003-01-30 2010-10-05 The United States Of America As Represented By The Secretary Of The Department Of Veterans Affairs Multilineage-inducible cells and uses thereof
US7638328B2 (en) 2003-05-08 2009-12-29 Cellartis Ab Method for efficient transfer of human blastocyst-derived stem cells (hBS cells) from a feeder-supported to a feeder-free culture system, long-term propagation of hBS cells under feeder-free conditions and use of cultured hBS cells for applications in myocardial regeneration
EP1627912A4 (en) * 2003-05-20 2007-12-12 Riken PREPARATION OF ENDODERMAL STEM CELLS
ES2584428T3 (es) * 2003-06-20 2016-09-27 Axiogenesis Ag Modelado de tejidos en un sistema de células madre embrionarias (ES)
US7875272B2 (en) * 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
AU2004252568B2 (en) 2003-06-27 2011-06-30 Ethicon, Incorporated Regeneration and repair of neural tissue using postpartum-derived cells
US8518390B2 (en) 2003-06-27 2013-08-27 Advanced Technologies And Regenerative Medicine, Llc Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells
US8790637B2 (en) 2003-06-27 2014-07-29 DePuy Synthes Products, LLC Repair and regeneration of ocular tissue using postpartum-derived cells
US9592258B2 (en) 2003-06-27 2017-03-14 DePuy Synthes Products, Inc. Treatment of neurological injury by administration of human umbilical cord tissue-derived cells
US8491883B2 (en) * 2003-06-27 2013-07-23 Advanced Technologies And Regenerative Medicine, Llc Treatment of amyotrophic lateral sclerosis using umbilical derived cells
US9572840B2 (en) 2003-06-27 2017-02-21 DePuy Synthes Products, Inc. Regeneration and repair of neural tissue using postpartum-derived cells
WO2005026335A2 (en) * 2003-09-15 2005-03-24 Ramot At Tel Aviv University Ltd. Insulin-producing bone marrow derived cells and methods of generating and using same
JP2007522796A (ja) * 2003-11-07 2007-08-16 ユニヴァーシティ オブ フロリダ リサーチ ファウンデーション,インク. 神経前駆細胞の培養と分化
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
WO2007059007A2 (en) 2005-11-14 2007-05-24 Cythera, Inc. Markers of definitive endoderm
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
AU2013200203B2 (en) * 2003-12-23 2014-11-20 Viacyte, Inc. Definitive endoderm
US7541185B2 (en) * 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
CN103898045B (zh) * 2003-12-23 2019-02-01 维亚希特公司 定形内胚层
WO2005072764A2 (en) * 2004-01-16 2005-08-11 Novocell, Inc. Fibrin-bound angiogenic factors to stimulate vascularization of transplant site of encapsulated cells
WO2005071066A1 (en) * 2004-01-23 2005-08-04 Board Of Regents, The University Of Texas System Methods and compositions for preparing pancreatic insulin secreting cells
CN1950498A (zh) * 2004-03-10 2007-04-18 加利福尼亚大学董事会 培养胚胎干细胞的组合物和方法
KR20070029681A (ko) * 2004-04-01 2007-03-14 위스콘신 얼럼나이 리서어치 화운데이션 줄기 세포의 내배엽 및 이자 혈통으로의 분화
KR101395516B1 (ko) * 2004-04-27 2014-05-14 비아싸이트, 인크. Pdx1 발현 내배엽
EP2377922B1 (en) * 2004-04-27 2020-04-08 Viacyte, Inc. PDX1 expressing endoderm
WO2006001471A1 (ja) * 2004-06-29 2006-01-05 The University Of Tokyo グルコース応答性インスリン分泌を促進するための医薬組成物
AU2011203583B2 (en) * 2004-07-09 2013-03-07 Viacyte, Inc. Methods for identifying factors for differentiating definitive endoderm
CA2573354C (en) * 2004-07-09 2015-02-24 Cythera, Inc. Preprimitive streak and mesendoderm cells
EP1809739B1 (en) 2004-07-13 2014-10-15 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
US8187878B2 (en) 2004-08-13 2012-05-29 University Of Georgia Research Foundation, Inc. Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors
JP2008514214A (ja) * 2004-09-29 2008-05-08 セルアーティス アーベー ヒト胚盤胞由来幹細胞(hBS)から肝細胞様細胞を発生させる方法
WO2006042132A2 (en) * 2004-10-08 2006-04-20 Georgia Tech Research Corporation Microencapsulation of cells in hydrogels using electrostatic potentials
WO2006040763A2 (en) * 2004-10-12 2006-04-20 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
US20060171930A1 (en) * 2004-12-21 2006-08-03 Agnieszka Seyda Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same
US20060153815A1 (en) * 2004-12-21 2006-07-13 Agnieszka Seyda Tissue engineering devices for the repair and regeneration of tissue
JP5425400B2 (ja) 2004-12-23 2014-02-26 エシコン・インコーポレイテッド 産褥由来細胞を用いた脳卒中および他の急性神経変性障害の治療
WO2006071911A2 (en) * 2004-12-23 2006-07-06 Cythera, Inc. Expansion of definitive endoderm cells
PL1831356T3 (pl) 2004-12-23 2017-07-31 DePuy Synthes Products, Inc. Komórki poporodowe pochodzące z tkanki pępowinowej i sposoby ich wytwarzania i stosowania
CA2593549C (en) * 2005-01-07 2016-04-26 Wake Forest University Health Sciences Regeneration of pancreatic islets by amniotic fluid stem cell therapy
JP2008528038A (ja) * 2005-01-31 2008-07-31 エス セル インターナショナル ピーティーイー リミテッド 胚性幹細胞の指示された分化及びその利用
CN100425694C (zh) * 2005-04-15 2008-10-15 北京大学 诱导胚胎干细胞向胰腺细胞分化的方法
EP1728873A1 (en) 2005-05-30 2006-12-06 DeveloGen Aktiengesellschaft Use of an insulinoma cell for identifying pancreatic beta-cell mitogens
AU2006202209B2 (en) * 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
AU2006255183B2 (en) * 2005-06-08 2012-02-02 Centocor, Inc. A cellular therapy for ocular degeneration
CN101233226B (zh) 2005-06-22 2017-08-11 阿斯特利亚斯生物治疗股份公司 人胚胎干细胞的悬浮培养物
US8476070B2 (en) 2005-08-29 2013-07-02 Technion Research & Development Foundation Limited Media for culturing stem cells
US20100322906A1 (en) * 2005-10-05 2010-12-23 Osaka University Method for Obtaining Pancreatic Endocrine Cells From Adipose Tissue-Origin Cells
NZ567082A (en) * 2005-10-14 2012-08-31 Univ Minnesota Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype
US8865763B2 (en) 2005-10-14 2014-10-21 Alltech, Inc. Methods and compositions for altering cell function
US8871715B2 (en) 2005-10-14 2014-10-28 Alltech, Inc. Use of selenium compounds, especially selenium yeasts for altering cognitive function
TWI319708B (en) * 2005-10-14 2010-01-21 Methods and compositions for altering cell fuction
AU2012202481B2 (en) * 2005-10-27 2015-07-02 Viacyte, Inc. PDX1-expressing dorsal and ventral foregut endoderm
EP3584311A1 (en) * 2005-10-27 2019-12-25 Viacyte, Inc. Pdx-1 expressing dorsal and ventral foregut endoderm
US9175261B2 (en) * 2005-12-16 2015-11-03 DePuy Synthes Products, Inc. Human umbilical cord tissue cells for inhibiting adverse immune response in histocompatibility-mismatched transplantation
ES2391034T3 (es) * 2005-12-19 2012-11-20 Ethicon, Inc. Expansión in vitro de células derivadas postparto en frascos rotatorios
SG170789A1 (en) * 2005-12-28 2011-05-30 Ethicon Inc Treatment of peripheral vascular disease using postpartum-derived cells
US9125906B2 (en) 2005-12-28 2015-09-08 DePuy Synthes Products, Inc. Treatment of peripheral vascular disease using umbilical cord tissue-derived cells
US11254916B2 (en) 2006-03-02 2022-02-22 Viacyte, Inc. Methods of making and using PDX1-positive pancreatic endoderm cells
AU2016213746A1 (en) * 2006-03-02 2016-08-25 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
AU2024201016B2 (en) * 2006-03-02 2024-10-24 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
EP2650359B1 (en) * 2006-03-02 2022-05-04 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
EP4438720A3 (en) * 2006-04-28 2025-02-26 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US7989204B2 (en) * 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
US20070259423A1 (en) * 2006-05-02 2007-11-08 Jon Odorico Method of differentiating stem cells into cells of the endoderm and pancreatic lineage
US8685730B2 (en) 2006-05-02 2014-04-01 Wisconsin Alumni Research Foundation Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
US20090298169A1 (en) * 2006-06-02 2009-12-03 The University Of Georgia Research Foundation Pancreatic and Liver Endoderm Cells and Tissue by Differentiation of Definitive Endoderm Cells Obtained from Human Embryonic Stems
GB2453074B (en) * 2006-06-02 2011-06-22 Geron Corp Differentiation of primate pluripotent cells to hepatocyte-lineage cells
WO2007149182A2 (en) * 2006-06-19 2007-12-27 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
US8874380B2 (en) 2010-12-09 2014-10-28 Rutgers, The State University Of New Jersey Method of overcoming therapeutic limitations of nonuniform distribution of radiopharmaceuticals and chemotherapy drugs
WO2008013664A2 (en) * 2006-07-26 2008-01-31 Cythera, Inc. Methods of producing pancreatic hormones
ES2874223T3 (es) 2006-08-02 2021-11-04 Technion Res & Dev Foundation Métodos de expansión de células madre embrionarias en un cultivo en suspensión
KR100885854B1 (ko) 2006-09-08 2009-02-26 고려대학교 산학협력단 인간배아 줄기세포로부터 췌장전구세포로의 분화유도 방법
WO2008048647A1 (en) * 2006-10-17 2008-04-24 Cythera, Inc. Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells
US9005964B2 (en) 2006-11-24 2015-04-14 Regents Of The University Of Minnesota Endodermal progenitor cells
EP2128261B1 (en) * 2006-12-20 2012-06-06 Shenzhen Chingen Genetic Engineering Co., Ltd. A recombinant adenovirus comprising recombinant khp53 gene and preparation method and uses thereof
WO2008156708A2 (en) * 2007-06-15 2008-12-24 Massachusetts Institute Of Technology Methods and compositions for enhanced differentiation from embryonic stem cells
US9080145B2 (en) * 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
ES2537578T3 (es) 2007-07-18 2015-06-09 Lifescan, Inc. Diferenciación de las células madre de embriones humanos
CN101848993A (zh) * 2007-07-19 2010-09-29 新加坡科技研究局 使胚胎干细胞分化成表达aqp-1的细胞的方法
BRPI0815052A2 (pt) 2007-07-31 2014-10-21 Lifescan Inc Diferenciação de células-tronco pluripotentes por meio de uso de células nutrizes humanas
CA2695225C (en) 2007-07-31 2021-06-01 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
BRPI0818183A2 (pt) * 2007-10-05 2019-07-16 Ethicon Inc reparo e regeneração do tecido renal usando células derivadas do tecido do cordão umbilical humano
DK2209888T3 (da) * 2007-10-12 2020-01-20 Astellas Inst For Regenerative Medicine Forbedrede fremgangsmåder til fremstilling af rpe-celler og sammensætninger af rpe-celler
US8198083B1 (en) 2007-10-31 2012-06-12 William Gunter Loudon Organotypic slices of the central nervous system
US9062290B2 (en) * 2007-11-27 2015-06-23 Lifescan, Inc. Differentiation of human embryonic stem cells
US8236538B2 (en) 2007-12-20 2012-08-07 Advanced Technologies And Regenerative Medicine, Llc Methods for sterilizing materials containing biologically active agents
BRPI0908033A2 (pt) 2008-02-21 2015-08-04 Centocor Ortho Biotech Inc Método placas de superfície modificada e composições para adesão, cultura e desprendimento de célula
WO2009124213A2 (en) * 2008-04-02 2009-10-08 The Trustees Of Columbia University In The City Of New York Dental stem cell differentiation
US8623648B2 (en) * 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
US20090280096A1 (en) * 2008-05-09 2009-11-12 Atsushi Kubo Pancreatic endocrine progenitor cells derived from pluripotent stem cells
AU2009248959A1 (en) * 2008-05-22 2009-11-26 Vesta Therapeutics, Inc. Method of differentiating mammalian progenitor cells into insulin producing pancreatic islet cells
GB2460552B (en) * 2008-06-05 2011-09-07 Iti Scotland Ltd Stem cell culture media and methods
BRPI0914116A2 (pt) 2008-06-30 2019-09-17 Centocor Ortho Biotech Inc diferenciação de células-tronco pluripotentes
MX2011000123A (es) * 2008-06-30 2011-02-25 Centocor Ortho Biotech Inc Diferenciacion de las celulas madre pluripotentes.
US20100028307A1 (en) * 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
KR101025880B1 (ko) * 2008-10-23 2011-03-30 공희숙 성체 줄기세포를 이용한 인슐린 분비세포로의 분화 유도방법
BRPI0919883A2 (pt) * 2008-10-31 2015-08-11 Centocor Ortho Biotech Inc Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática
EP2346988B1 (en) * 2008-10-31 2017-05-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
IL289685B2 (en) * 2008-11-04 2025-09-01 Viacyte Inc Stem cell pool suspension preparations and methods for their differentiation
CN105349517B (zh) 2008-11-14 2021-05-04 维赛特公司 源于人多能干细胞的胰腺细胞的包封
ES2642070T3 (es) 2008-11-20 2017-11-15 Janssen Biotech, Inc. Cultivo de células madre pluripotentes en microportadores
CN102257132B (zh) 2008-11-20 2014-09-03 森托科尔奥索生物科技公司 用于在平面基底上进行细胞附着和培养的方法和组合物
WO2010071864A1 (en) 2008-12-19 2010-06-24 Ethicon, Incorporated Treatment of lung and pulmonary diseases and disorders
US10179900B2 (en) * 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
EP3061808B1 (en) * 2009-02-03 2020-08-12 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells
WO2010091241A2 (en) 2009-02-06 2010-08-12 President And Fellows Of Harvard College Compositions and methods for promoting the generation of definitive endoderm
WO2010111663A1 (en) 2009-03-26 2010-09-30 Ethicon, Inc. Human umbilical cord tissue cells as therapy for alzheimer' s disease
EP2378272B1 (en) * 2009-06-12 2013-05-15 Olympus Corporation Subject information analysis device and subject information analysis method
WO2010151733A1 (en) * 2009-06-25 2010-12-29 The Trustees Of Columbia University In The City Of New York Dental stem cell reprogramming
EP2446021A4 (en) * 2009-06-25 2013-11-13 Geron Corp DIFFERENTIATED PLURIPOTENTIAL STEM CELL PROMOTION DERIVED WITH IRRELEVANT PHENOTYPES
WO2011005326A1 (en) 2009-07-09 2011-01-13 Massachusetts Institute Of Technology Methods and compositions for increased safety of stem cell-derived populations
BR112012001480A2 (pt) 2009-07-20 2015-09-01 Janssen Biotech Inc Diferenciação de células-tronco embriônicas humanas
AU2010276402B2 (en) * 2009-07-20 2014-07-03 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
AU2010276440B2 (en) * 2009-07-20 2014-07-03 Janssen Biotech Inc. Differentiation of human embryonic stem cells
JP5957382B2 (ja) * 2009-10-29 2016-07-27 ヤンセン バイオテツク,インコーポレーテツド 多能性幹細胞
WO2011058558A2 (en) 2009-11-12 2011-05-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2011079017A2 (en) 2009-12-23 2011-06-30 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
EP2516626B1 (en) * 2009-12-23 2017-05-10 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CA2785966C (en) 2009-12-29 2020-10-27 Takeda Pharmaceutical Company Limited Method for manufacturing pancreatic-hormone-producing cells
WO2011109279A2 (en) 2010-03-01 2011-09-09 Centocor Ortho Biotech Inc. Methods for purifying cells derived from pluripotent stem cells
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
EP3498825A1 (en) * 2010-05-12 2019-06-19 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US20110312001A1 (en) 2010-06-15 2011-12-22 Emile Nuwaysir Compendium of ready-built stem cell models for interrogation of biological response
EP2601289B1 (en) 2010-08-04 2017-07-12 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
CA2807935C (en) * 2010-08-09 2018-09-18 Takeda Pharmaceutical Company Limited Method of producing pancreatic hormone-producing cells
DK2603226T3 (da) 2010-08-12 2021-06-07 Janssen Biotech Inc Behandling af diabetes med endokrine pankreas-prækursorceller
ES2659393T3 (es) 2010-08-31 2018-03-15 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas
RU2673946C1 (ru) 2010-08-31 2018-12-03 Янссен Байотек, Инк. Дифференцирование плюрипотентных стволовых клеток
US9506036B2 (en) 2010-08-31 2016-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP2630232A4 (en) 2010-10-22 2014-04-02 Biotime Inc METHOD FOR MODIFYING TRANSCRIPTIONAL REGULATORY NETWORKS IN STEM CELLS
US10000739B2 (en) * 2010-10-28 2018-06-19 National University Corporation Kumamoto University Method and culture medium for improving pluripotent stem cell differentiation inducing efficiency
GB2502704B (en) 2010-11-15 2019-12-04 Accelerated Biosciences Corp Generation of neural stem cells from human trophoblast stem cells
EP2652125B1 (en) * 2010-12-15 2017-04-26 Kadimastem Ltd. Insulin producing cells derived from pluripotent stem cells
KR20140004707A (ko) 2011-01-31 2014-01-13 독립행정법인 국립국제의료연구 센터 다능성 줄기 세포 유래 고기능 간세포와 그의 제조 방법 및 약제 대사 독성 시험 방법
EP2691512B1 (en) 2011-03-29 2019-05-01 Asterias Biotherapeutics, Inc. Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells
JP6312591B2 (ja) 2011-06-21 2018-04-18 ノヴォ ノルディスク アー/エス 多能性幹細胞からの胚体内胚葉の効率的な誘導
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
US20150174202A1 (en) * 2011-09-28 2015-06-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and Compositions for Modulating Beta Cell Proliferation
US9670463B2 (en) 2011-10-14 2017-06-06 Children's Medical Center Corporation Inhibition and enhancement of reprogramming by chromatin modifying enzymes
EP2794857A4 (en) 2011-12-22 2015-07-08 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS IN SINGLE INSULIN POSITIVE CELLS
US9611513B2 (en) 2011-12-23 2017-04-04 DePuy Synthes Products, Inc. Detection of human umbilical cord tissue derived cells
HK1206058A1 (en) 2012-03-07 2015-12-31 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
US9447378B2 (en) 2012-04-27 2016-09-20 Massachusetts Institute Of Technology Method for differentiating human embryonic stem cells into β-cells for the treatment of type I diabetes
US9416705B2 (en) 2012-04-27 2016-08-16 Hino Motors, Ltd. Burner and filter renewal device
DK2844739T3 (da) * 2012-04-30 2019-09-30 Univ Health Network FREMGANGSMÅDER OG SAMMENSÆTNINGER TIL GENERERING AF PANCREATISKE PROGENITORER OG FUNKTIONELLE BETA-CELLER UD FRA hPSC'ER
CN104284977A (zh) 2012-05-07 2015-01-14 詹森生物科技公司 人胚胎干细胞向胰腺内胚层的分化
BR112014028881A2 (pt) 2012-05-23 2017-06-27 Hoffmann La Roche populações de células, banco de células, métodos de obtenção de uma população de células, métodos de identificação de um fator, métodos de seleção, métodos de fornecimento de terapia, populações de hepatócitos e método de obtenção de células
ES2897649T3 (es) * 2012-06-08 2022-03-02 Janssen Biotech Inc Diferenciación de células madre embrionarias humanas en células endocrinas pancreáticas
JP5979452B2 (ja) * 2012-06-11 2016-08-24 国立大学法人北海道大学 多能性幹細胞の選別方法
GB201216796D0 (en) * 2012-09-20 2012-11-07 Cambridge Entpr Ltd In vitro pancreatic differentiation
CA2886027C (en) 2012-09-25 2023-03-07 Yale University Differentiation of human ips cells to human alveolar type ii via definitive endoderm
CA3137404A1 (en) 2012-11-30 2014-06-05 Accelerated Biosciences Corp. Pancreatic progenitor cells expressing betatrophin and insulin
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
EP4219683A1 (en) * 2012-12-31 2023-08-02 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
AU2013368221B2 (en) 2012-12-31 2018-11-01 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
EP2961829A4 (en) 2013-02-27 2016-08-17 Univ California GENERATION OF THYME PEPPER THERAPEUTIC CELLS IN VITRO
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
KR102341674B1 (ko) 2013-06-11 2021-12-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
SG11201510209SA (en) 2013-06-13 2016-01-28 Orgenesis Ltd Cell populations, methods of transdifferention and methods of use thereof
WO2014201431A1 (en) 2013-06-14 2014-12-18 Massachusetts Institute Of Technology Articles and methods for stem cell differentiation
GB201317869D0 (en) 2013-10-09 2013-11-20 Cambridge Entpr Ltd In vitro production of foregut stem cells
US9988605B2 (en) 2013-12-11 2018-06-05 Korea Advanced Institute Of Science And Technology Method for preparing endocrine aggregate of insulin-producing beta cells from human pluripotent stem cells
BR112016013623B1 (pt) * 2013-12-16 2023-03-21 Fresenius Medical Care Deutschland Gmbh Método de preparação de uma estrutura de célula esferoide semelhante à ilhota pancreática cultivada artificialmente, e uso de uma estrutura de célula esferoide
US9804692B2 (en) * 2014-03-28 2017-10-31 Samsung Display Co., Ltd. System and method for generating haptic feedback in stylus
CN106459918A (zh) 2014-04-24 2017-02-22 得克萨斯州大学系统董事会 施加诱导多能干细胞产生过继细胞疗法产品
US10597633B2 (en) 2014-05-16 2020-03-24 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for organoids
CA2949056C (en) 2014-05-16 2025-10-07 Janssen Biotech, Inc. USE OF SMALL MOLECULES TO IMPROVE MAFA GENE EXPRESSION IN PANCREATIC ENDOCRINE CELLS
EP3149156B1 (en) 2014-05-28 2021-02-17 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
CN107075467B (zh) * 2014-09-19 2020-08-21 新加坡科技研究局 由干细胞分化肝细胞样细胞
US11584916B2 (en) 2014-10-17 2023-02-21 Children's Hospital Medical Center Method of making in vivo human small intestine organoids from pluripotent stem cells
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
CN113234661A (zh) 2014-12-18 2021-08-10 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
US10190096B2 (en) 2014-12-18 2019-01-29 President And Fellows Of Harvard College Methods for generating stem cell-derived β cells and uses thereof
KR102281752B1 (ko) 2014-12-19 2021-07-23 얀센 바이오테크 인코포레이티드 만능성 줄기 세포의 현탁 배양
MA41296A (fr) 2014-12-30 2017-11-07 Orgenesis Ltd Procédés de transdifférenciation et procédés d'utilisation de ceux-ci
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
CN116790476A (zh) 2016-05-05 2023-09-22 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
KR102546194B1 (ko) 2016-11-04 2023-06-21 칠드런즈 호스피탈 메디칼 센터 간 유사 장기 조성물 및 이를 제조 및 사용하는 방법
KR102558606B1 (ko) 2016-12-05 2023-07-26 칠드런즈 호스피탈 메디칼 센터 결장 유사장기 및 이를 제조 및 사용하는 방법
US20190390169A1 (en) 2017-03-03 2019-12-26 Kyoto University Pancreatic progenitor cell production method
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
WO2018191673A1 (en) 2017-04-14 2018-10-18 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
SG11201911256UA (en) 2017-05-08 2020-01-30 Orgenesis Ltd Transdifferentiated cell populations and methods of use thereof
EP3638205A4 (en) 2017-06-14 2021-03-17 Semma Therapeutics, Inc. DEVICES AND METHODS FOR ADMINISTERING THERAPEUTICS
JP2020535834A (ja) 2017-10-03 2020-12-10 エスディーエフ バイオファーマ インコーポレイテッド 遺伝子修飾されたベータ細胞による糖尿病の治療
EP3694603A4 (en) 2017-10-10 2021-07-14 Children's Hospital Medical Center ESOPHAGUS TISSUE AND / OR ORGANOID COMPOSITIONS AND METHOD FOR MANUFACTURING THEREOF
KR102789149B1 (ko) 2017-11-15 2025-03-31 셈마 테라퓨틱스, 인크. 섬세포 제조 조성물 및 사용 방법
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
JP7506657B2 (ja) 2018-07-26 2024-06-26 チルドレンズ ホスピタル メディカル センター 肝胆膵組織およびその作製方法
AU2019320072A1 (en) 2018-08-10 2021-02-25 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
US12428622B2 (en) 2018-09-12 2025-09-30 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
GB201819224D0 (en) 2018-11-26 2019-01-09 Koninklijke Nederlandse Akademie Van Wetenschappen Hepatocyte expansion methods
JP7610533B2 (ja) 2019-05-31 2025-01-08 チルドレンズ ホスピタル メディカル センター 造血幹細胞の生成および拡大方法
CN112852627A (zh) * 2019-11-28 2021-05-28 中国科学院大连化学物理研究所 一种基于多器官芯片的多能干细胞来源人肝和胰岛共培养的方法
CN113106054B (zh) * 2020-01-13 2022-10-14 青岛瑞思德生物科技有限公司 一种将间充质干细胞诱导分化为胰岛细胞的诱导剂
US12188051B2 (en) 2020-05-27 2025-01-07 The Regents Of The University Of Michigan Compositions and methods for obtaining vascularized human intestinal organoid tissue, and related uses thereof
US12076437B2 (en) 2021-07-12 2024-09-03 Brown University Proangiogenic protein cocktails delivered in custom biomaterials to revascularize ischemic tissue
WO2025117331A1 (en) 2023-12-01 2025-06-05 Eli Lilly And Company Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4391909A (en) * 1979-03-28 1983-07-05 Damon Corporation Microcapsules containing viable tissue cells
WO1998014592A2 (en) * 1996-10-01 1998-04-09 Geron Corporation Telomerase reverse transcriptase
US5902577A (en) * 1993-09-30 1999-05-11 Merck Patent Gesellsschaft Mit Beschrankter Haftung Insulin-secreting cell lines, methods of production and use
WO2000047720A2 (en) * 1999-02-10 2000-08-17 Curis, Inc. Pancreatic progenitor cells, methods and uses related thereto
WO2002079457A1 (en) * 2001-03-29 2002-10-10 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic differentiation pathway

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4378016A (en) 1981-07-15 1983-03-29 Biotek, Inc. Artificial endocrine gland containing hormone-producing cells
US4439521A (en) 1981-10-21 1984-03-27 Ontario Cancer Institute Method for producing self-reproducing mammalian pancreatic islet-like structures
GB8604497D0 (en) 1986-02-24 1986-04-03 Connaught Lab Separation of islets of langerhans
GB2197915B (en) 1986-11-19 1990-11-14 Rolls Royce Plc Improvements in or relating to fluid bearings
ES2179821T3 (es) * 1991-06-24 2003-02-01 Hcell Technology Inc Celulas pancreaticas secretoras de hormonas y mantenimiento en cultivo a largo plazo.
US5425764A (en) 1992-07-30 1995-06-20 The University Of Toledo Bioartificial pancreas
AU687386B2 (en) 1993-04-08 1998-02-26 Human Cell Cultures, Inc. Cell culturing method and medium
US5834308A (en) * 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US5587309A (en) * 1994-04-29 1996-12-24 The United States Of America As Represented By The Department Of Health And Human Services Method of stimulating proliferation and differentiation of human fetal pancreatic cells ex vivo
US5849989A (en) * 1994-10-07 1998-12-15 Ontogeny, Inc. Insulin promoter factor, and uses related thereto
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5679565A (en) * 1995-04-10 1997-10-21 The Regents Of The University Of California Method of preserving pancreatic islets
AU6496996A (en) 1996-02-23 1997-09-10 Circle Biomedical, Inc. Novel artificial pancreas
US5869243A (en) 1996-03-05 1999-02-09 Rhode Island Hospital Immortalized hepatocytes
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ,インコーポレイテッド 胚性幹細胞血清置換
US6090622A (en) * 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US5935852A (en) * 1997-07-03 1999-08-10 Genetics Institute, Inc. DNA molecules encoding mammalian cerberus-like proteins
JP3880795B2 (ja) 1997-10-23 2007-02-14 ジェロン・コーポレーション フィーダー細胞を含まない培養物中で、霊長類由来始原幹細胞を増殖させるための方法
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6946293B1 (en) * 1999-02-10 2005-09-20 Es Cell International Pte Ltd. Progenitor cells, methods and uses related thereto
WO2000047721A2 (en) 1999-02-10 2000-08-17 Ontogeny, Inc. Methods of inducing insulin positive progenitor cells
US6774120B1 (en) 1999-06-01 2004-08-10 Sarah Ferber Methods of inducing regulated pancreatic hormone production in non-pancreatic islet tissues
AU5632600A (en) 1999-06-23 2001-01-09 Joslin Diabetes Center Inc. Methods of making pancreatic islet cells
JP5398941B2 (ja) 1999-08-05 2014-01-29 エイビーティー ホールディング カンパニー 多能性成体幹細胞およびそれを単離する方法
AU7445800A (en) 1999-09-27 2001-04-30 Chugai Seiyaku Kabushiki Kaisha Method of prolonging normal cell life span
CA2392615A1 (en) 1999-12-06 2001-06-07 The General Hospital Corporation Pancreatic stem cells and their use in transplantation
WO2001051610A1 (en) 2000-01-14 2001-07-19 Bresagen Limited Ectoderm cell production
US6610535B1 (en) * 2000-02-10 2003-08-26 Es Cell International Pte Ltd. Progenitor cells and methods and uses related thereto
US6436704B1 (en) * 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
CA2435826A1 (en) 2001-01-24 2002-08-01 The Government Of The United States Of America Differentiation of stem cells to pancreatic endocrine cells
AU2002235728A1 (en) 2001-02-26 2002-10-03 Novo Nordisk A/S Method for generating insulin-secreting cells suitable for transplantation
WO2002074948A2 (en) 2001-03-21 2002-09-26 Geron Corporation Animal tissue with carbohydrate antigens compatible for human transplantation
US20050054102A1 (en) * 2001-04-19 2005-03-10 Anna Wobus Method for differentiating stem cells into insulin-producing cells
EP1393066A4 (en) * 2001-05-15 2006-01-25 Rappaport Family Inst For Res INSULIN-PRODUCING CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS
CA2451838A1 (en) 2001-05-25 2002-12-05 Cythera, Inc. Stem cell differentiation
EP1298201A1 (en) * 2001-09-27 2003-04-02 Cardion AG Process for the production of cells exhibiting an islet-beta-cell-like state
EP1448763A4 (en) 2001-11-09 2006-11-29 Es Cell Int Pte Ltd CHARACTERIZATION AND ISOLATION OF SUBSETS OF HUMAN EMBRYONIC STEM CELLS (HES) AND CELLS ASSOCIATED THERETO OR DERIVED THEREFROM
KR101008868B1 (ko) * 2001-12-07 2011-01-17 제론 코포레이션 인간 배아 줄기세포 유래의 섬세포
EP2361966A1 (en) 2002-05-17 2011-08-31 Mount Sinai School of Medicine of New York University Mesoderm and definitive endoderm cell populations
US20060003446A1 (en) * 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
AU2003273573A1 (en) 2002-05-28 2003-12-19 Becton, Dickinson And Company Expansion and transdifferentiation of human acinar cells
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
CN103898045B (zh) 2003-12-23 2019-02-01 维亚希特公司 定形内胚层
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
JP2008528038A (ja) * 2005-01-31 2008-07-31 エス セル インターナショナル ピーティーイー リミテッド 胚性幹細胞の指示された分化及びその利用
WO2007075807A2 (en) 2005-12-20 2007-07-05 Es Cell International Pte Ltd. Methods for the directed differentiation of embryonic stem cell
WO2007149182A2 (en) 2006-06-19 2007-12-27 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4391909A (en) * 1979-03-28 1983-07-05 Damon Corporation Microcapsules containing viable tissue cells
US5902577A (en) * 1993-09-30 1999-05-11 Merck Patent Gesellsschaft Mit Beschrankter Haftung Insulin-secreting cell lines, methods of production and use
WO1998014592A2 (en) * 1996-10-01 1998-04-09 Geron Corporation Telomerase reverse transcriptase
WO2000047720A2 (en) * 1999-02-10 2000-08-17 Curis, Inc. Pancreatic progenitor cells, methods and uses related thereto
WO2002079457A1 (en) * 2001-03-29 2002-10-10 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic differentiation pathway

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Diabetes, Vol 48, 1999, BO Roep et al, "Auto- and alloimmune reactivity to human islet allografts...", 484-490 *
Diabetes, Vol 49, 2000, B Soria et al, "Insulin-secreting cells derived from embryonic stem cells normalize glycemia in Streptozotocin-induced diabetic mice", 1-6 *
Diabetes, Vol 50, 2000, S Assady et al, "Insulin production by human embryonic stem cells", 1691-1697 *
Diabetologia, Vol 41, 1998, B Keymeulen et al, "Implantation of standardized beta-cell grafts in a liver segment of IDDM patients...", 452-459 *

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