BRPI0815052A2 - Diferenciação de células-tronco pluripotentes por meio de uso de células nutrizes humanas - Google Patents

Diferenciação de células-tronco pluripotentes por meio de uso de células nutrizes humanas

Info

Publication number
BRPI0815052A2
BRPI0815052A2 BRPI0815052-4A2A BRPI0815052A BRPI0815052A2 BR PI0815052 A2 BRPI0815052 A2 BR PI0815052A2 BR PI0815052 A BRPI0815052 A BR PI0815052A BR PI0815052 A2 BRPI0815052 A2 BR PI0815052A2
Authority
BR
Brazil
Prior art keywords
nutrie
differentiation
human
stem cells
pluripotent stem
Prior art date
Application number
BRPI0815052-4A2A
Other languages
English (en)
Inventor
John J O'neil
Original Assignee
Lifescan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lifescan Inc filed Critical Lifescan Inc
Publication of BRPI0815052A2 publication Critical patent/BRPI0815052A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/22—Coculture with; Conditioned medium produced by pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
BRPI0815052-4A2A 2007-07-31 2008-07-31 Diferenciação de células-tronco pluripotentes por meio de uso de células nutrizes humanas BRPI0815052A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95293707P 2007-07-31 2007-07-31
PCT/US2008/071775 WO2009048675A1 (en) 2007-07-31 2008-07-31 Pluripotent stem cell differentiation by using human feeder cells

Publications (1)

Publication Number Publication Date
BRPI0815052A2 true BRPI0815052A2 (pt) 2014-10-21

Family

ID=40193704

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0815052-4A2A BRPI0815052A2 (pt) 2007-07-31 2008-07-31 Diferenciação de células-tronco pluripotentes por meio de uso de células nutrizes humanas

Country Status (11)

Country Link
EP (2) EP2185691B1 (pt)
JP (2) JP6087043B2 (pt)
KR (1) KR101592180B1 (pt)
CN (1) CN102317443B (pt)
BR (1) BRPI0815052A2 (pt)
CA (1) CA2694956C (pt)
ES (2) ES2648128T3 (pt)
PL (2) PL2185691T3 (pt)
RU (1) RU2010107181A (pt)
WO (1) WO2009048675A1 (pt)
ZA (1) ZA201001436B (pt)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5269612B2 (ja) 2006-02-07 2013-08-21 スパイナルサイト， エルエルシー インビボバイオリアクターを使用する軟骨の修復のための方法および組成物
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CN101952415B (zh) 2007-07-31 2017-06-27 生命扫描有限公司 人胚胎干细胞的分化
CN107574142B (zh) 2007-11-27 2021-07-06 生命扫描有限公司 人胚胎干细胞的分化
WO2009105570A2 (en) 2008-02-21 2009-08-27 Centocor Ortho Biotech Inc. Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
JP5734183B2 (ja) 2008-06-30 2015-06-17 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞の分化
BRPI0919885A2 (pt) 2008-10-31 2015-08-11 Centocor Ortho Biotech Inc Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática
CA2742267C (en) 2008-10-31 2019-06-04 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
AU2009316583B2 (en) 2008-11-20 2016-04-21 Janssen Biotech, Inc. Methods and compositions for cell attachment and cultivation on planar substrates
RU2555538C2 (ru) 2008-11-20 2015-07-10 Сентокор Орто Байотек Инк. Культура плюрипотентных стволовых клеток на микроносителях
EP2456862A4 (en) 2009-07-20 2013-02-27 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
CN102741395B (zh) 2009-12-23 2016-03-16 詹森生物科技公司 人胚胎干细胞的分化
AU2011223900A1 (en) 2010-03-01 2012-09-13 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
MX351515B (es) 2010-05-12 2017-10-17 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas.
US9528090B2 (en) 2010-08-31 2016-12-27 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
AU2011296381B2 (en) 2010-08-31 2016-03-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CA2809305C (en) 2010-08-31 2019-06-11 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
CN102732479B (zh) * 2011-03-31 2014-08-27 北京大学 胸腺上皮前体细胞的制备方法及其专用培养基
ES2902650T3 (es) 2011-06-21 2022-03-29 Novo Nordisk As Inducción eficiente de endodermo definitivo a partir de células madre pluripotentes
EP2776556B1 (en) * 2011-11-09 2017-11-08 Spinalcyte, LLC Dermal fibroblasts for treatment of degenerative disc disease
AU2012355698B2 (en) 2011-12-22 2018-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
WO2013122864A1 (en) * 2012-02-14 2013-08-22 Washington State University Research Foundation Feeder-free method for culture of bovine and porcine spermatogonial stem cells
KR20140131999A (ko) 2012-03-07 2014-11-14 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 증폭 및 유지를 위한 한정 배지
CN104428410B (zh) * 2012-05-25 2016-08-31 学校法人埼玉医科大学 胰激素产生细胞的制造方法及胰激素产生细胞、以及分化诱导促进剂
CN108103006A (zh) 2012-06-08 2018-06-01 詹森生物科技公司 人胚胎干细胞向胰腺内分泌细胞的分化
US20150247123A1 (en) 2012-09-03 2015-09-03 Novo Nordisk A/S Generation of pancreatic endoderm from Pluripotent Stem cells using small molecules
EP2913396B1 (en) * 2012-10-29 2018-08-29 Saitama Medical University Method of manufacturing differentiated pluripotent stem cell
KR102036780B1 (ko) 2012-12-31 2019-10-25 얀센 바이오테크 인코포레이티드 Hb9 조절제를 사용하는 인간 배아 줄기세포의 췌장 내분비 세포로의 분화
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
KR102084561B1 (ko) 2012-12-31 2020-03-04 얀센 바이오테크 인코포레이티드 췌장 내분비 세포로의 분화를 위한 공기-액체 계면에서의 인간 배아 줄기세포의 배양
JP6529440B2 (ja) 2012-12-31 2019-06-12 ヤンセン バイオテツク，インコーポレーテツド 膵内分泌細胞への分化のためのヒト多能性細胞の懸濁及びクラスタリング
JP2014161257A (ja) * 2013-02-22 2014-09-08 Univ Of Tokyo 多能性幹細胞から膵ランゲルハンス島を製造する方法
JP6352908B2 (ja) 2013-05-24 2018-07-04 学校法人 埼玉医科大学 新規ペプチド及びその用途
JP2016523083A (ja) 2013-06-19 2016-08-08 スパイナルサイト， エルエルシー 軟骨細胞適用のための脂肪細胞
WO2015004762A1 (ja) * 2013-07-10 2015-01-15 株式会社日立製作所 細胞培養方法、装置、及び細胞シート
EP3031905A4 (en) 2013-08-07 2017-04-26 Kyoto University Method for producing pancreatic hormone-producing cell
US10114008B2 (en) 2014-01-21 2018-10-30 Empire Technology Development Llc Methods and devices for high throughput screening of conditions affecting stem cell differentiation
CA2949056A1 (en) 2014-05-16 2015-11-19 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
WO2016183141A1 (en) * 2015-05-11 2016-11-17 Alfred E. Mann Institute For Biomedical Engineering At The University Of Southern California Pancreatic stromal progenitor cells
CN107779433A (zh) * 2016-08-30 2018-03-09 天津市康婷生物工程有限公司 便捷的刺激nk细胞增殖和分化的饲养层制备方法
KR102095346B1 (ko) * 2018-11-16 2020-03-31 고려대학교 산학협력단 삼차원 공배양을 통한 신생혈관능이 증가된 이식용 지방줄기세포 시트 및 이의 제조방법
EP3975926A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates, Inc. A biocompatible membrane composite
CN114401752B (zh) 2019-05-31 2023-04-04 W.L.戈尔及同仁股份有限公司 具有受控氧扩散距离的细胞封装装置
CN114173837A (zh) 2019-05-31 2022-03-11 W.L.戈尔及同仁股份有限公司 生物相容性膜复合材料
CA3139591C (en) 2019-05-31 2024-01-16 Timothy M. BRUHN A biocompatible membrane composite
US20230072771A1 (en) 2019-10-30 2023-03-09 Astellas Institute For Regenerative Medicine Methods for producing retinal pigment epithelium cells
JP7284516B2 (ja) 2020-12-14 2023-05-31 合同会社オフィス・ゼロ プログラム作成支援システム及びその方法並びにそのプログラム
CN114891734A (zh) * 2022-06-02 2022-08-12 四川农业大学 一种永生化的牦牛瘤胃成纤维细胞系及其构建和应用

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834308A (en) 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6458593B1 (en) 1999-01-21 2002-10-01 Vitro Diagnostics, Inc. Immortalized cell lines and methods of making the same
US20030082155A1 (en) 1999-12-06 2003-05-01 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
EP1240518A4 (en) 1999-12-13 2006-05-17 Scripps Research Inst MARKERS FOR THE IDENTIFICATION AND INSULATION OF PRE-GENERIC CELLS OF A AND B PANCREAS ISOLATED CELLS
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US7439064B2 (en) 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
CA2456981C (en) 2001-08-06 2012-02-28 Bresagen, Inc. Alternative compositions and methods for the culture of stem cells
KR101089591B1 (ko) 2001-12-07 2011-12-05 제론 코포레이션 인간 배아 줄기세포 유래의 섬세포
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
JP2005527241A (ja) 2002-05-28 2005-09-15 ベクトン・ディキンソン・アンド・カンパニー ｉｎｖｉｔｒｏにおけるヒト膵臓腺房細胞の増殖およびインスリン産生細胞への分化転換のための方法
US6877147B2 (en) 2002-07-22 2005-04-05 Broadcom Corporation Technique to assess timing delay by use of layout quality analyzer comparison
US20040110287A1 (en) 2002-07-29 2004-06-10 Es Cell International Pte Ltd. Multi-step method for the differentiation of insulin positive, glucose responsive cells
WO2004090110A2 (en) 2003-03-31 2004-10-21 Bresagen Inc. Compositions and methods for the control, differentiation and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway
ITRM20030395A1 (it) 2003-08-12 2005-02-13 Istituto Naz Per Le Malattie Infettive Lazz Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero.
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US20050233446A1 (en) 2003-12-31 2005-10-20 Parsons Xuejun H Defined media for stem cell culture
WO2005086845A2 (en) 2004-03-10 2005-09-22 Regents Of The University Of California Compositions and methods for growth of embryonic stem cells
CA2966883A1 (en) * 2004-07-09 2006-02-16 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
CN101188942B (zh) 2005-03-04 2011-11-30 生命扫描有限公司 成年胰衍生的基质细胞
JP5092124B2 (ja) * 2005-05-24 2012-12-05 国立大学法人 熊本大学 Ｅｓ細胞の分化誘導方法
WO2007030870A1 (en) * 2005-09-12 2007-03-22 Es Cell International Pte Ltd Cardiomyocyte production
WO2007082963A1 (es) * 2006-01-18 2007-07-26 Fundación Instituto Valenciano De Infertilidad Líneas de células madre embrionarias humanas y métodos para usar las mismas
AU2007224116B2 (en) * 2006-03-02 2013-11-07 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production

Also Published As

Publication number Publication date
CA2694956A1 (en) 2009-04-16
CN102317443A (zh) 2012-01-11
ES2665434T3 (es) 2018-04-25
RU2010107181A (ru) 2011-09-20
PL2185691T3 (pl) 2018-08-31
EP2584034B1 (en) 2017-10-25
JP2010535035A (ja) 2010-11-18
EP2584034A1 (en) 2013-04-24
CN102317443B (zh) 2018-09-14
EP2185691B1 (en) 2018-03-14
ES2648128T3 (es) 2017-12-28
KR20100061455A (ko) 2010-06-07
ZA201001436B (en) 2011-06-29
PL2584034T3 (pl) 2018-03-30
JP6087043B2 (ja) 2017-03-01
KR101592180B1 (ko) 2016-02-05
EP2185691A1 (en) 2010-05-19
EP2584034B8 (en) 2017-12-06
WO2009048675A1 (en) 2009-04-16
JP2016208984A (ja) 2016-12-15
JP6453277B2 (ja) 2019-01-16
CA2694956C (en) 2017-12-19

Similar Documents

Publication Publication Date Title
BRPI0815052A2 (pt) Diferenciação de células-tronco pluripotentes por meio de uso de células nutrizes humanas
BRPI0814425A2 (pt) Diferenciação de células-tronco embrionárias humanas
BRPI0813787A2 (pt) Cultura de célula-tronco pluripotente isolada
BRPI0814894A2 (pt) Diferenciação de células-tronco embrionárias de ser humano.
GB2458863B (en) Improved culture of stem cells
ZA201606403B (en) Pluripotent stem cell culture on micro-carriers
BRPI0913925A2 (pt) Diferenciação de células-tronco pluripotentes
BR112012001564A2 (pt) Diferenciação de células tronco embrionárias humanas.
GB2441718B (en) Differentiation of human embryonic stem cells to cardiomyocyte-lineage cells
BR112012001480A2 (pt) Diferenciação de células-tronco embriônicas humanas
HK1156665A1 (en) Differentiation of pluripotent stem cells
BR112013017632A2 (pt) meio de cultura celular
BR112012014158A2 (pt) "célula eletroquímica"
FI20096288A0 (fi) Formulations and methods for culturing stem cells
DK2574677T3 (da) Fremgangsmåder til celledyrkning
EP2487236A4 (en) CULTURE SUPPORT FOR HUMAN PLURIPOTENTE STEM CELLS AND USE THEREOF
IL214529A0 (en) Bioreactor for the cultivation of mammalian cells and methods of use thereof
PL2046946T3 (pl) Hodowla pluripotencjalnych komórek macierzystych
IL187352A0 (en) Establishment of a human embryonic stem cell line using mammalian cells
GB0622394D0 (en) Differentiation of pluripotent cells
IT1395109B1 (it) Bioreattore per la stimolazione di cellule staminali
IL205610A0 (en) Methods of measuring cell viability without using control cells
BRPI1010684A2 (pt) método para determinar o potencial cardiorregenerativo de células mamíferas
EP2294182A4 (en) CULTS OF MULTIPOTENT STEM CELLS
DK2061877T3 (da) Differentiering af stamceller

Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 12A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2594 DE 24-09-2020 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.

B350 Update of information on the portal [chapter 15.35 patent gazette]