DK2844739T3 - FREMGANGSMÅDER OG SAMMENSÆTNINGER TIL GENERERING AF PANCREATISKE PROGENITORER OG FUNKTIONELLE BETA-CELLER UD FRA hPSC'ER - Google Patents

FREMGANGSMÅDER OG SAMMENSÆTNINGER TIL GENERERING AF PANCREATISKE PROGENITORER OG FUNKTIONELLE BETA-CELLER UD FRA hPSC'ER Download PDF

Info

Publication number
DK2844739T3
DK2844739T3 DK13784648.1T DK13784648T DK2844739T3 DK 2844739 T3 DK2844739 T3 DK 2844739T3 DK 13784648 T DK13784648 T DK 13784648T DK 2844739 T3 DK2844739 T3 DK 2844739T3
Authority
DK
Denmark
Prior art keywords
hpscs
compositions
methods
beta cells
pancreatic progenitors
Prior art date
Application number
DK13784648.1T
Other languages
English (en)
Inventor
Gordon Keller
Maria Cristina Nostro
Audrey Holtzinger
Farida Sarangi
Original Assignee
Univ Health Network
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Health Network filed Critical Univ Health Network
Application granted granted Critical
Publication of DK2844739T3 publication Critical patent/DK2844739T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Endocrinology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK13784648.1T 2012-04-30 2013-04-30 FREMGANGSMÅDER OG SAMMENSÆTNINGER TIL GENERERING AF PANCREATISKE PROGENITORER OG FUNKTIONELLE BETA-CELLER UD FRA hPSC'ER DK2844739T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261640366P 2012-04-30 2012-04-30
PCT/CA2013/000432 WO2013163739A1 (en) 2012-04-30 2013-04-30 METHODS AND COMPOSITIONS FOR GENERATING PANCREATIC PROGENITORS AND FUNCTIONAL BETA CELLS FROM hPSCs

Publications (1)

Publication Number Publication Date
DK2844739T3 true DK2844739T3 (da) 2019-09-30

Family

ID=49514141

Family Applications (1)

Application Number Title Priority Date Filing Date
DK13784648.1T DK2844739T3 (da) 2012-04-30 2013-04-30 FREMGANGSMÅDER OG SAMMENSÆTNINGER TIL GENERERING AF PANCREATISKE PROGENITORER OG FUNKTIONELLE BETA-CELLER UD FRA hPSC'ER

Country Status (18)

Country Link
US (1) US20150104430A1 (da)
EP (2) EP2844739B1 (da)
JP (2) JP6397397B2 (da)
CN (2) CN108004199B (da)
AU (1) AU2013255031C1 (da)
CA (1) CA2871795C (da)
CY (1) CY1122058T1 (da)
DK (1) DK2844739T3 (da)
ES (1) ES2747967T3 (da)
HK (2) HK1208047A1 (da)
HR (1) HRP20191677T1 (da)
HU (1) HUE046271T2 (da)
IN (1) IN2014DN10053A (da)
LT (1) LT2844739T (da)
PL (1) PL2844739T3 (da)
PT (1) PT2844739T (da)
SI (1) SI2844739T1 (da)
WO (1) WO2013163739A1 (da)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105358680B (zh) * 2013-04-03 2019-06-25 富士费勒姆细胞动力学有限公司 用于悬浮培养内胚层祖细胞的方法和组合物
CN106536718B (zh) * 2014-05-21 2021-04-27 国立大学法人京都大学 胰芽细胞的制造方法及含有胰芽细胞的胰疾病治疗剂
PT3286300T (pt) 2015-04-24 2020-12-28 Univ Copenhagen Isolamento de células progenitoras pancreáticas genuínas
ES2856872T3 (es) * 2015-04-24 2021-09-28 Univ Copenhagen Procedimiento para la producción de células productoras de insulina
EP3328404A4 (en) * 2015-07-27 2018-12-26 The Regents of The University of California Methods and compositions for producing pancreatic beta cells
CN106554936B (zh) * 2015-09-30 2020-05-05 海门雨霖细胞科技有限责任公司 诱导人干细胞向肝细胞定向分化的新方法
WO2017144695A1 (en) * 2016-02-24 2017-08-31 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
MA45329A (fr) 2016-04-08 2019-02-13 Univ California Production de cellules bêta matures pleinement fonctionnelles à partir de progénitrices pancréatiques humaines
WO2018021293A1 (ja) * 2016-07-26 2018-02-01 国立大学法人京都大学 Ｐｔｆ１ａ陽性細胞の製造方法
US20190390169A1 (en) * 2017-03-03 2019-12-26 Kyoto University Pancreatic progenitor cell production method
CN111246864A (zh) * 2017-07-21 2020-06-05 森玛治疗公司 干细胞衍生的胰腺β细胞的重聚
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
CA3081762A1 (en) 2017-11-15 2019-05-23 Semma Therapeutics, Inc. Islet cell manufacturing compositions and methods of use
CA3088968C (en) 2018-01-18 2023-10-17 Daiichi Sankyo Company, Limited Dihydroindolizinone derivative
EP3801577A4 (en) 2018-05-31 2021-07-14 University Health Network (UHN) METHOD AND COMPOSITIONS USING TANKYRASE INHIBITOR FOR GENERATING INSULIN-PRODUCING CELLS
AU2019320072A1 (en) 2018-08-10 2021-02-25 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
CA3110932A1 (en) 2018-08-30 2020-03-05 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
CN111875675B (zh) * 2018-09-03 2021-10-01 广东新征程生命科学有限公司 表皮干细胞向胰腺细胞分化的改进方法
CN112888778A (zh) * 2018-10-15 2021-06-01 Cynity株式会社 通过低分子化合物由源自内胚层组织或器官的细胞制备干细胞/祖细胞的方法
JP7385244B2 (ja) * 2019-06-27 2023-11-22 国立大学法人 東京大学 膵前駆細胞の分離方法
WO2021051256A1 (en) * 2019-09-17 2021-03-25 Center For Excellence In Molecular Cell Science, Chinese Academy Of Sciences Pancreatic pro-endocrine progenitor cells and use thereof
CN113234664B (zh) * 2021-05-11 2024-05-10 澳门大学 一种胰腺祖细胞的制备方法及其应用
CN114634904B (zh) * 2022-05-17 2022-09-13 天津外泌体科技有限公司 高纯度胰腺祖细胞的产生方法
CN118345030B (zh) * 2024-06-20 2024-08-16 羽铂精制生物技术(成都)有限公司 iPSC诱导分化成为胰腺祖细胞修复胰腺的方法及应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
GB2415432B (en) * 2001-12-07 2006-09-06 Geron Corp Islet cells from human embryonic stem cells
JP5102030B2 (ja) 2004-08-13 2012-12-19 ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド ヒト胚性幹細胞における自己再生および分化のための組成物および方法
EP1999253B1 (en) 2006-03-02 2019-05-22 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US7989204B2 (en) 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
US20080063628A1 (en) * 2006-09-11 2008-03-13 Davis Janet E Methods to promote cell differentiation
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US8338170B2 (en) * 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
GB201111244D0 (en) * 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
US9150833B2 (en) * 2009-12-23 2015-10-06 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2012070014A2 (en) * 2010-11-26 2012-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Identification of novel cell surface markers for pancreatic progenitor cells and definite endodermal cells

Also Published As

Publication number Publication date
HK1254968A1 (zh) 2019-08-02
US20150104430A1 (en) 2015-04-16
JP6397397B2 (ja) 2018-09-26
CN104411815B (zh) 2017-11-28
JP2018198620A (ja) 2018-12-20
JP6759293B2 (ja) 2020-09-23
EP2844739A4 (en) 2015-12-02
CN108004199A (zh) 2018-05-08
LT2844739T (lt) 2019-10-10
CY1122058T1 (el) 2020-11-25
AU2013255031C1 (en) 2018-12-06
PL2844739T3 (pl) 2019-12-31
HUE046271T2 (hu) 2020-02-28
EP2844739B1 (en) 2019-06-19
EP3594323A1 (en) 2020-01-15
CN104411815A (zh) 2015-03-11
AU2013255031A1 (en) 2014-12-04
HRP20191677T1 (hr) 2019-12-13
HK1208047A1 (en) 2016-02-19
CA2871795C (en) 2023-03-14
ES2747967T3 (es) 2020-03-12
WO2013163739A1 (en) 2013-11-07
AU2013255031B2 (en) 2018-09-06
IN2014DN10053A (da) 2015-08-14
CA2871795A1 (en) 2013-11-07
PT2844739T (pt) 2019-10-01
JP2015519048A (ja) 2015-07-09
EP2844739A1 (en) 2015-03-11
CN108004199B (zh) 2022-03-18
SI2844739T1 (sl) 2019-11-29

Similar Documents

Publication Publication Date Title
DK2844739T3 (da) FREMGANGSMÅDER OG SAMMENSÆTNINGER TIL GENERERING AF PANCREATISKE PROGENITORER OG FUNKTIONELLE BETA-CELLER UD FRA hPSC'ER
DK3008172T3 (da) Dyrkningsmedium-sammensætninger til modning af cardiomyocytter afledt fra pluripotente mammale stamceller
DK3693384T3 (da) Fremgangsmåde til at generere T-celler med kompatibilitet for allogen transplantation
DK2971167T3 (da) Sammensætninger og fremgangsmåder til targeting af nucleinsyre med nucleinsyrer
DK3926051T3 (da) Fremgangsmåder til høst af pattedyrecellekulturer
DK2855667T3 (da) Fremgangsmåder til manipulation af allogeniske og immunosuppressiv-resistante T-celler til immunterapi
DK3597749T5 (da) Fremgangsmåder og sammensætninger til rna-dirigeret mål-dna-modificering og til rna-dirigeret modulering af transkription
DK3238709T3 (da) Forbedrede parenterale formuleringer af lipofile farmaceutiske midler og fremgangsmåder til fremstilling og anvendelse af samme
DK3109326T3 (da) Sammensætning og fremgangsmåde til nukleinsyresyntese og amplifikation under anvendelse af rt
DK2928921T3 (da) Sammensætninger og fremgangsmåder til antistoffer målrettet epo
DK2554160T3 (da) Fremgangsmåde til fremstilling af en beriget IgG-sammensætning fra plasma
DK3428290T3 (da) Sammensætninger og fremgangsmåder til amplifikation af nukleinsyrer
DK2718270T3 (da) Sammensætninger og fremgangsmåder til fremstillingen af pyrimidin- og pyridinforbindelser med btk-hæmmende aktivitet
DK2793600T3 (da) Smeltelig røgfri tobakssammensætning
DK2800565T3 (da) Carbamatforbindelser og fremgangsmåder til fremstilling og anvendelse af samme
DK3421051T3 (da) Fremgangsmåder til glycokonjugering og sammensætninger
DK3444342T3 (da) Fremgangsmåder og sammensætninger til behandlingen af lysosomale aflejringssygdomme
DK3027186T3 (da) Fremgangsmåde til fremstilling af sammensætninger, der omfatter hyaluronsyre og mepivacainhydrochlorid
DK4082530T3 (da) Proteasehæmmerholdige sammensætninger, sammensætninger omfattende samme og fremgangsmåder til fremstilling og anvendelse af samme
DK2893001T3 (da) Fremgangsmåde til dyrkning af mesenkymale stamceller
DK3165087T3 (da) Midesammensætning, bærer, fremgangsmåde til opdræt af mider og anvendelser relateret dertil
DK2968673T3 (da) Fremgangsmåde til decellularisering af vævstransplantater
DK2934187T3 (da) Økonomisk fremgangsmåde til isolering af funktionelt protein fra planter
DK2922955T3 (da) Sammensætninger og fremgangsmåder til modulering af FGFR3-ekspression
DK2917358T3 (da) Sammensætninger og fremgangsmåder til biologisk fremstilling af fedtsyrederivater