FI94911B - Förfarande för bestämning av ett immunologiskt aktivt ämne och vid förfarandet användbar reagens - Google Patents

Förfarande för bestämning av ett immunologiskt aktivt ämne och vid förfarandet användbar reagens Download PDF

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Publication number
FI94911B
FI94911B FI911750A FI911750A FI94911B FI 94911 B FI94911 B FI 94911B FI 911750 A FI911750 A FI 911750A FI 911750 A FI911750 A FI 911750A FI 94911 B FI94911 B FI 94911B
Authority
FI
Finland
Prior art keywords
antigen
antibody
reagent
reaction
immunologically active
Prior art date
Application number
FI911750A
Other languages
English (en)
Finnish (fi)
Other versions
FI911750A0 (sv
FI94911C (sv
Inventor
Shoichi Shutoh
Akinori Suginaka
Mikio Akita
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of FI911750A0 publication Critical patent/FI911750A0/sv
Publication of FI94911B publication Critical patent/FI94911B/sv
Application granted granted Critical
Publication of FI94911C publication Critical patent/FI94911C/sv

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/826—Additives, e.g. buffers, diluents, preservatives

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Claims (3)

1. Förfarande för bestämmande av en immunologiskt aktiv substans, väri en antigen-antikroppreaktion i en 5 vätska används, kännetecknat därav, att man i vätskan tillsätter en förening med den allmänna formeln: ^0-( (CH2CH20 )„( AO )n}-R2 10 väri R1 och R2 envar är en väteatom eller en kolvätegrupp med 1-5 kolatomer, AO är en oxialkylengrupp med 3-4 kolatomer, m och n betecknar respektive antal oxietylen-grupper och oxialkylengrupper, varvid oxietylen- och oxi-alkylengrupperna sampolykondenserats slumpartat för erhäl-15 lande av en molekylvikt frän 1 000 - 20 000 och att för-hällandet m/n ligger inom omrädet 60/40 - 90/10.
2. Förfarande enligt patentkravet 1, kännetecknat därav, att bestämningen utförs optiskt.
3. Reagens för immunologisk bestämning av en immu-20 nologiskt aktiv substans, kännetecknad därav, att den innehäller en förening med en allmänna formeln: R10-{ (CH2CH20)o( A0)n}-R2 (I) .. 25 väri R1 och R2 är envar en väteatom eller en kolvätegrupp med 1-5 kolatomer, AO är en oxialkylengrupp med 3-4 kolatomer, m och n betecknar respektive antal oxietylen-grupper och oxialkylengrupper, varvid oxietylen- och oxialkylengrupperna sampolykondenserats slumpartat för 30 erhällande av en molekylvikt frän 1 000 - 20 000 och att ·. förhällandet m/n ligger i omrädet 60/40 - 90/10.
FI911750A 1988-10-13 1991-04-11 Förfarande för bestämning av ett immunologiskt aktivt ämne och vid förfarandet användbar reagens FI94911C (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP63257846A JP2684069B2 (ja) 1988-10-13 1988-10-13 免疫学的活性物質の測定方法及びそのための試薬
JP25784688 1988-10-13
JP8901051 1989-10-12
PCT/JP1989/001051 WO1990004179A1 (en) 1988-10-13 1989-10-12 Method for assaying immunologically active substance and reagent therefor

Publications (3)

Publication Number Publication Date
FI911750A0 FI911750A0 (sv) 1991-04-11
FI94911B true FI94911B (sv) 1995-07-31
FI94911C FI94911C (sv) 1995-11-10

Family

ID=17311964

Family Applications (1)

Application Number Title Priority Date Filing Date
FI911750A FI94911C (sv) 1988-10-13 1991-04-11 Förfarande för bestämning av ett immunologiskt aktivt ämne och vid förfarandet användbar reagens

Country Status (11)

Country Link
US (1) US5296355A (sv)
EP (1) EP0439611B1 (sv)
JP (1) JP2684069B2 (sv)
KR (1) KR0160103B1 (sv)
AT (1) ATE111603T1 (sv)
AU (1) AU630376B2 (sv)
DE (1) DE68918286T2 (sv)
DK (1) DK66491D0 (sv)
FI (1) FI94911C (sv)
NO (1) NO301615B1 (sv)
WO (1) WO1990004179A1 (sv)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4023671A1 (de) * 1990-07-25 1992-01-30 Boehringer Mannheim Gmbh Nichtionische blockcopolymere aus propylenoxid und ethylenoxid
US5627080A (en) * 1994-07-29 1997-05-06 Beckman Instruments, Inc. Detergent-facilitated immunoassay for the rapid and quantitative assay of pharmacological agents
DE4439348A1 (de) * 1994-11-04 1996-05-09 Boehringer Mannheim Gmbh Weiße Trigger-Präparationen zur Verbesserung der Signaldetektion bei bio- und chemilumineszenten Reaktionen
EP0850402A4 (en) * 1996-07-03 1998-12-09 Anna P Jaklitsch PRETREATMENT REAGENTS AND RELATED METHODS
JP4219491B2 (ja) * 1999-06-18 2009-02-04 富士フイルム株式会社 乾式分析方法及び乾式分析要素
JP6242068B2 (ja) * 2013-04-10 2017-12-06 積水メディカル株式会社 ラテックス免疫凝集測定試薬

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS357594B1 (sv) * 1958-05-20 1960-06-21
BE787398A (fr) * 1971-08-10 1973-02-12 Basf Ag Alcools oxalcoyles et preparations detergentes les contenant comme anti-mousses
US3862243A (en) * 1972-02-17 1975-01-21 Int Flavors & Fragrances Inc Mixed oxyalkylates employed as antifoamers
US3853465A (en) * 1972-06-09 1974-12-10 Technicon Instr Turbidity reduction in serum and plasma samples using polyoxyethylated lauric acid compounds
US3880989A (en) * 1973-01-30 1975-04-29 Baxter Laboratories Inc Production of antisera comprising fractionating plasma or serum with an ethylene oxide-polyoxypropylene block copolymer
US4148869A (en) * 1975-03-06 1979-04-10 Baxter Travenol Laboratories, Inc. Immunological reagent and method of using same
IL48804A (en) * 1975-01-29 1979-05-31 Baxter Travenol Lab Imminological reagent comprising a mixture of polyethyleneglycol and a nonionic surfactant
JPS5311997A (en) * 1976-07-20 1978-02-02 Kuraray Co Ltd Preparation of etherified polyoxyalkylene
DE2927170C2 (de) * 1979-07-05 1984-01-19 Schill & Seilacher GmbH & Co, 7030 Böblingen Präparationsmittel zur Herstellung von synthetischen Filamenten
DE2918826A1 (de) * 1979-05-10 1980-11-27 Basf Ag Verwendung von alkoxylierten alkoholen als biologisch abbaubare, schaumarme tenside in wasch- und reinigungsmitteln
JPS6331448Y2 (sv) * 1980-07-01 1988-08-23
US4452903A (en) * 1981-02-17 1984-06-05 Lee Jin P Assay method and reagent kit means for lipid-containing body fluid
US4454232A (en) * 1982-06-07 1984-06-12 Bio-Rad Laboratories, Inc. Estriol assay
JPS5943362A (ja) * 1982-09-06 1984-03-10 Kainosu:Kk 免疫学的測定試薬
JPS604938U (ja) * 1983-06-23 1985-01-14 三井造船株式会社 自動採水装置
DE3327642A1 (de) * 1983-07-30 1985-02-14 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur bestimmung eines partners einer immunreaktion sowie reagens zur durchfuehrung dieses verfahrens
GB2145726A (en) * 1983-08-26 1985-04-03 Diversey Corp Surface active agents
DE3532626A1 (de) * 1985-09-12 1987-03-19 Boehringer Mannheim Gmbh Verfahren zur bestimmung eines partners einer immunreaktion
US4810630A (en) * 1987-03-30 1989-03-07 E. I. Du Pont De Nemours And Company Use of polyoxyethylene ethers to improve the performance of immunoassays employing peroxidase conjugates

Also Published As

Publication number Publication date
EP0439611B1 (en) 1994-09-14
WO1990004179A1 (en) 1990-04-19
EP0439611A1 (en) 1991-08-07
KR0160103B1 (ko) 1999-03-30
JPH02103466A (ja) 1990-04-16
DE68918286D1 (de) 1994-10-20
DK66491A (da) 1991-04-12
US5296355A (en) 1994-03-22
DK66491D0 (da) 1991-04-12
DE68918286T2 (de) 1995-02-23
FI911750A0 (sv) 1991-04-11
FI94911C (sv) 1995-11-10
NO911444D0 (no) 1991-04-12
NO911444L (no) 1991-05-16
EP0439611A4 (en) 1992-03-11
AU4348989A (en) 1990-05-01
JP2684069B2 (ja) 1997-12-03
KR900702369A (ko) 1990-12-06
ATE111603T1 (de) 1994-09-15
AU630376B2 (en) 1992-10-29
NO301615B1 (no) 1997-11-17

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