FI88515B - Enzymelektrod - Google Patents

Enzymelektrod Download PDF

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Publication number
FI88515B
FI88515B FI880300A FI880300A FI88515B FI 88515 B FI88515 B FI 88515B FI 880300 A FI880300 A FI 880300A FI 880300 A FI880300 A FI 880300A FI 88515 B FI88515 B FI 88515B
Authority
FI
Finland
Prior art keywords
electrode
enzyme
platinum
electrodes
resin
Prior art date
Application number
FI880300A
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English (en)
Finnish (fi)
Other versions
FI880300A0 (fi
FI88515C (sv
FI880300A (fi
Inventor
Hugh Peter Bennetto
Gerard Michael Delaney
Jeremy Richard Mason
Christopher Frank Thurston
John Laing Stirling
David Robert Dekeyzer
Original Assignee
Cambridge Life Sciences
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Filing date
Publication date
Application filed by Cambridge Life Sciences filed Critical Cambridge Life Sciences
Publication of FI880300A0 publication Critical patent/FI880300A0/fi
Publication of FI880300A publication Critical patent/FI880300A/fi
Publication of FI88515B publication Critical patent/FI88515B/fi
Application granted granted Critical
Publication of FI88515C publication Critical patent/FI88515C/sv

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • C12Q1/002Electrode membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • C12Q1/005Enzyme electrodes involving specific analytes or enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • C12Q1/005Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006Enzyme electrodes involving specific analytes or enzymes for glucose
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/817Enzyme or microbe electrode

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Inert Electrodes (AREA)
  • Catalysts (AREA)

Claims (9)

1. Enzymelektrod, vilken förmär svara amperometriskt mot den katalytiskä aktiviteten av enzymet i närvaro av sitt respektive substrat och vilken omfattar ett oxidoreduk-tasenzym, vilket är immobiliserat eller adsorberat pk ett elektriskt ledande stödelement, vilket bestir av eller omfattar ett poröst skikt av hartsbundna koi- eller grafitpartiklar och vilka partiklar är i beröring med en platinagruppmetall i grundämnesform, känneteck-n a d därav att platinagruppmetalIen i grundämnesform 38 8 8 51 S utgörs av finfördelade partiklar jämnt dispergerade över tjockleken av koi- eller grafitskiktet och att nämnda partiklar förefinns i nämnda skikt antingen som en vä-sentligen jämn blandning av nämnda koi- eller grafitpar-tiklar och nämnda finfördelade platinagruppmetall, eller som finfördelade partiklar av platinagruppmetallen i grundämnesform, vilka är föradsorberade pä ytan av kol-eller grafitpartiklarna före bindningen vid nämnda harts.
2. Enzymelektrod enligt patentkravet 1, känne- t e c k n a d därav att platinagruppmetallen är platina eller palladium.
3. Enzymelektrod enligt patentkravet l eller 2, k ä n -netecknad därav att det syntetiska hartsbinde-medlet är fluorkolharts eller polyvinylacetat.
4. Enzymelektrod enligt patentkravet 3, k ä n netecknad därav att den syntetiska hartsen är poly-tetrafluoretylen.
5. Enzymelektrod enligt patentkravet l, k ä n netecknad därav att oxidoreduktaset är glukosoxidas.
6. Enzymelektrod enligt n&got av patentkraven 1-5, kännetecknad därav att det elektriskt ledande stödelementet omfattar en elektriskt ledande bas, vid vilken de nämnda hartsbundna koi- eller grafitpartiklarna är bundna som ett ytskikt som en blandning med nämnda finmalade platinagruppmetall, eller pä vars yta nämnda metall är adsorberad eller utfälld.
7. Enzymelektrod enligt patentkravet 6, kännetecknad därav att det elektriskt ledande stödelementet är ett elektrikst ledande kolpapper.
8. Enzymelektrod enligt nägot av patentkraven 1-7, 39 8851 5 kännetecknad därav att det porösa skiktet omfattar hartsbundna koi- eller grafitpartiklar, varvid en platinagruppmetall är utfälld eller adsorberad pä ytan av de enskilda partiklarna Innan blndning med hartsen.
9. Enzymelektrod enligt patentkravet 8, kännetecknad därav att det porösa skiktet omfattar hartsbundet platinabelagt kolpulver, vars partikelstorlek är inom omrädet 5 - 30 nm och pä vars yta kolloidal platina är adsoberad, vars partikelstorlek är 1,5 - 2,5 nm.
FI880300A 1986-05-27 1988-01-22 Enzymelektrod FI88515C (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB8612861 1986-05-27
GB868612861A GB8612861D0 (en) 1986-05-27 1986-05-27 Immobilised enzyme biosensors
PCT/GB1987/000365 WO1987007295A1 (en) 1986-05-27 1987-05-27 Immobilised enzyme electrodes
GB8700365 1987-05-27

Publications (4)

Publication Number Publication Date
FI880300A0 FI880300A0 (fi) 1988-01-22
FI880300A FI880300A (fi) 1988-01-22
FI88515B true FI88515B (fi) 1993-02-15
FI88515C FI88515C (sv) 1993-05-25

Family

ID=10598501

Family Applications (1)

Application Number Title Priority Date Filing Date
FI880300A FI88515C (sv) 1986-05-27 1988-01-22 Enzymelektrod

Country Status (17)

Country Link
US (1) US4970145A (sv)
EP (1) EP0247850B1 (sv)
KR (1) KR950004906B1 (sv)
AU (1) AU591565B2 (sv)
CA (1) CA1303132C (sv)
DE (1) DE3785485T2 (sv)
DK (1) DK173722B1 (sv)
ES (1) ES2041264T3 (sv)
FI (1) FI88515C (sv)
GB (2) GB8612861D0 (sv)
HU (1) HU202577B (sv)
IE (1) IE60371B1 (sv)
IL (1) IL82601A (sv)
MX (1) MX171340B (sv)
NO (1) NO176920C (sv)
RU (1) RU1801119C (sv)
WO (1) WO1987007295A1 (sv)

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IE871360L (en) 1987-11-27
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GB8712445D0 (en) 1987-07-01
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RU1801119C (ru) 1993-03-07
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