FI110006B - Hiivamutantit, joilla on vajavaisuus N-glykosylaatiossa - Google Patents

Hiivamutantit, joilla on vajavaisuus N-glykosylaatiossa Download PDF

Info

Publication number
FI110006B
FI110006B FI933487A FI933487A FI110006B FI 110006 B FI110006 B FI 110006B FI 933487 A FI933487 A FI 933487A FI 933487 A FI933487 A FI 933487A FI 110006 B FI110006 B FI 110006B
Authority
FI
Finland
Prior art keywords
dsm
yeast
god
mutants
glycosylation
Prior art date
Application number
FI933487A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI933487A0 (fi
FI933487A (fi
Inventor
Erhard Kopetzki
Ludwig Lehle
Klaus Lehnert
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of FI933487A0 publication Critical patent/FI933487A0/fi
Publication of FI933487A publication Critical patent/FI933487A/fi
Application granted granted Critical
Publication of FI110006B publication Critical patent/FI110006B/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
FI933487A 1992-08-07 1993-08-06 Hiivamutantit, joilla on vajavaisuus N-glykosylaatiossa FI110006B (fi)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE4226094 1992-08-07
DE4226094 1992-08-07
DE4301932 1993-01-25
DE4301932A DE4301932A1 (de) 1992-08-07 1993-01-25 Hefewirtsstämme mit Defekten in der N-Glycosylierung

Publications (3)

Publication Number Publication Date
FI933487A0 FI933487A0 (fi) 1993-08-06
FI933487A FI933487A (fi) 1994-02-08
FI110006B true FI110006B (fi) 2002-11-15

Family

ID=25917284

Family Applications (1)

Application Number Title Priority Date Filing Date
FI933487A FI110006B (fi) 1992-08-07 1993-08-06 Hiivamutantit, joilla on vajavaisuus N-glykosylaatiossa

Country Status (12)

Country Link
US (2) US5705616A (ja)
EP (1) EP0582244B1 (ja)
JP (2) JP2675511B2 (ja)
KR (1) KR0140973B1 (ja)
AT (1) ATE207955T1 (ja)
AU (1) AU657230B2 (ja)
CA (1) CA2103522C (ja)
DE (2) DE4301932A1 (ja)
FI (1) FI110006B (ja)
IL (1) IL106589A (ja)
NO (1) NO312246B1 (ja)
NZ (1) NZ248323A (ja)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2750434B1 (fr) * 1996-06-26 1998-08-21 Applic De Rech Et De Conseils Produit de stabilisation proteique des vins
US6566122B1 (en) 2000-06-16 2003-05-20 Academia Sinica Super-secreting saccharomyces cerevisiae strains
CA2411968C (en) * 2000-06-30 2009-12-15 Flanders Interuniversity Institute For Biotechnology (Vib) Protein glycosylation modification in pichia pastoris
KR100473658B1 (ko) * 2002-10-25 2005-03-10 드림바이오젠 주식회사 위치 선택적 수식법에 의한 혼성형 단백질 치료제 개발을 위한 형질전환 효모 시스템
US7507573B2 (en) 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
US8202523B2 (en) * 2005-09-22 2012-06-19 ProSci, Inc. Glycosylated polypeptides produced in yeast mutants and methods of use thereof
KR101460561B1 (ko) * 2012-11-20 2014-11-13 한국식품연구원 사카로마이세스 세레비지애 113-8의 대량 생산방법
WO2017170418A1 (ja) * 2016-03-30 2017-10-05 株式会社カネカ エンドヌクレアーゼ活性を有するポリペプチド及びその製造方法
CN107988177B (zh) * 2016-10-26 2021-08-20 潍坊康地恩生物科技有限公司 葡萄糖氧化酶突变体
CN108118037B (zh) * 2016-11-28 2021-08-31 青岛蔚蓝生物集团有限公司 一种耐热性提高的葡萄糖氧化酶突变体

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU625392B2 (en) * 1987-10-29 1992-07-09 Zymogenetics Inc. Methods of regulating protein glycosylation
US5135854A (en) * 1987-10-29 1992-08-04 Zymogenetics, Inc. Methods of regulating protein glycosylation
NZ229260A (en) * 1988-06-03 1994-02-25 Merck & Co Inc Hepatitis b virus, expression cassette for pre-s domain, host cells and
US5094951A (en) * 1988-06-21 1992-03-10 Chiron Corporation Production of glucose oxidase in recombinant systems
EP0382332A3 (en) * 1989-01-06 1990-11-14 Collaborative Research Inc. Improved supersecreting mutants of saccharomyces cerevisiae
CA2022109A1 (en) * 1989-07-31 1991-02-01 Cheryl A. Schulman Enhanced expression of heterologous proteins in recombinant hosts in a non-growth media
DE4301904A1 (de) * 1992-08-07 1994-02-10 Boehringer Mannheim Gmbh Hypoglycosylierte recombinante Glucoseoxidase

Also Published As

Publication number Publication date
KR940004051A (ko) 1994-03-14
DE59310233D1 (de) 2001-12-06
US5705616A (en) 1998-01-06
CA2103522A1 (en) 1994-02-08
EP0582244B1 (de) 2001-10-31
JPH1052277A (ja) 1998-02-24
JP2675511B2 (ja) 1997-11-12
NZ248323A (en) 1994-12-22
EP0582244A3 (de) 1995-03-15
JP2984229B2 (ja) 1999-11-29
AU4435493A (en) 1994-02-24
AU657230B2 (en) 1995-03-02
FI933487A0 (fi) 1993-08-06
DE4301932A1 (de) 1994-02-10
ATE207955T1 (de) 2001-11-15
IL106589A (en) 2005-07-25
NO932811D0 (no) 1993-08-06
CA2103522C (en) 1998-07-07
JPH06296482A (ja) 1994-10-25
KR0140973B1 (ko) 1998-06-15
FI933487A (fi) 1994-02-08
NO932811L (no) 1994-02-08
US5798226A (en) 1998-08-25
NO312246B1 (no) 2002-04-15
IL106589A0 (en) 1993-12-08
EP0582244A2 (de) 1994-02-09

Similar Documents

Publication Publication Date Title
Tachibana et al. The yeast EUG1 gene encodes an endoplasmic reticulum protein that is functionally related to protein disulfide isomerase
JP5052899B2 (ja) 遺伝子発現法
KR101298157B1 (ko) 2-미크론 패밀리 플라스미드 및 이의 용도
US7488591B2 (en) Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
Nagasu et al. Isolation of new temperature‐sensitive mutants of Saccharomyces cerevisiae deficient in mannose outer chain elongation
MXPA04012983A (es) Modificacion mediante glucosilacion de proteina en pastoris pichia.
FI110006B (fi) Hiivamutantit, joilla on vajavaisuus N-glykosylaatiossa
US5602018A (en) Hypoglycosylated recombinant glucose oxidases
FI100408B (fi) Proteiinien glykosylaation säätelymenetelmiä
KR100915670B1 (ko) 야로위아 리폴리티카 유래의 신규 ＹｌＭＰＯ１ 유전자 및이의 파쇄 균주를 이용한 만노스 인산이 제거된 당단백질생산 방법
AU683061B2 (en) Yeast strain and methods for expressing heterologous proteins in yeast
JPH09266792A (ja) 酵母のマンノース−１−リン酸転移を正に制御する遺伝子ならびにこの遺伝子の欠損変異株を利用する高マンノース型中性糖鎖の製造方法
KR100672745B1 (ko) 효모 발현시스템에서의 재조합 외래단백질의 분비효율을향상시키는 방법
JPH06277086A (ja) 哺乳類の高マンノース型糖蛋白質糖鎖の酵母による製造法
KR20040004089A (ko) 당외쇄 합성 결손 한세눌라 폴리모르파 변이주 및 그를이용한 재조합 당단백질의 제조 방법
Gorgens Quantitative yeast physiology and nitrogen metabolism during heterologous protein production
JPH0279982A (ja) 真核細胞系表現ベクター、及び真核宿主細胞中での相同及び非相同蛋白質又は蛋白質含有遺伝子生産物の調節可能な製法
JPH06253858A (ja) げっ歯類アポリポプロテインｅを有する融合遺伝子、それを挿入したプラスミドおよびその用途

Legal Events

Date Code Title Description
HC Name/ company changed in application

Owner name: ROCHE DIAGNOSTICS GMBH

MA Patent expired