KR940004051A - N-글리코실화에 결함이 있는 효모 숙주 균주 - Google Patents

N-글리코실화에 결함이 있는 효모 숙주 균주 Download PDF

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KR940004051A
KR940004051A KR1019930015252A KR930015252A KR940004051A KR 940004051 A KR940004051 A KR 940004051A KR 1019930015252 A KR1019930015252 A KR 1019930015252A KR 930015252 A KR930015252 A KR 930015252A KR 940004051 A KR940004051 A KR 940004051A
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yeast
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레흐레 루드빅
레너르트 클라우스
코페츠키 에르하르트
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콜프, 융
뵈링거 만하임 게엠베하
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Abstract

본 발명은 〔3H〕-만노오스 자멸선택, 하나 또는 여러개의 선택적 마아커의 도입, 플라스미드 YEpL/GOD를 이용한 형질전환 및 표준조건하에 배양된 후 배양배자로 10㎖/ℓ 또는 그 이상의 GOD를 분리하고, 맥주효모 DSM7042, DSM 7338, DSM 7160 및/또는 DSM 7340의 ngd 돌연변이에 대립성이고 균일한 탄수화물 구조를 갖는 단백질을 분비하는 균주들의 선택에 의해 얻을 수 있는 N-글리코실화에 결함이 있는 효모 균주에 관한 것이다.

Description

Ｎ-글리코실화에 결함이 있는 효모 숙주 균주 [도면의 간단한 설명]
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 α-글루코시다아제 벡터인 XEp/5c6b3(12466 염시쌍)의 제한지도이고,
제2도는 맥주효모군에서 아스페르킬루스 니거 GOD의 분비를 위한 플라스미드를제조하기 위한 출발벡터인 XEpL(10717염기쌍;효모 발현벡터)의 제한지도이며,
제3도는 본 발명의 플라스미드 YEpL/GOD(12528 염기쌍)의 제한지도이다.

Claims (7)

  1. 〔3H〕-만노오스 자멸선택, 하나 또는 여러개의 선택적 마아커(영양요구성 및/또는 내성)의 도입, 플라스미드 YEpL/GOD를 이용한 형질전환 및 2% 효모추출물, 4% 박트펩톤(Difco), 0.1mod/ℓ 인산염완충액, pH7.0, 1% 프룩토오스 및 6%말토오스를 포함하는 완전배지에서 발효된 후 진동되면서 3일 내지 4일동안 인큐베이션된 후 배지로 10㎖/ℓ 또는 그 이상의 GOD를 분리하고, 맥주효모균 DSM7042, DSM 7338, DSM 7160 및/또는 DSM 7340에 대립성인 균주들의 선택에 의해 얻을 수 있는 N-글리코실화에 결함이 있는 효모 균주.
  2. 효모돌이연변이체, DSM7042, DSM 7338, DSM 7160 및 DSM 7340.
  3. 〔3H〕-만노오스 자멸선택, 하나 또는 여러개의 선택적 마아커(영양요구성 및/또는 내성)의 도입, 플라스미드 YEpL/GOD를 이용한 형질전환 및 2% 효모추출물, 4% 박트펩톤(Difco), 0.1mod/ℓ 인산염완충액, pH7.0, 1% 프룩토오스 및 6%말토오스를 포함하는 완전배지에서 발효된 후 진동되면서 3일 내지 4일동안 인큐베이션된 후 배지로 10㎖/ℓ 또는 그 이상의 GOD를 분리하고, 맥주효모균 DSM7042, DSM 7338, DSM 7160 및/또는 DSM 7340에 대립성인 균주들의 선택단계로 이루어지는 제1항 및 2항 기재의 효모 돌이변이체들의 제조방법.
  4. 제3항에 있어서, 맥주효모균 DSM7042, DSM 7338, DSM 7160 및/또는 DSM 7340의 제조를 위한 방법.
  5. 단백질의 동종 및 이종발현을 위한 숙주 유기체로서의 제1항 또는 2항의 표는 돌연변이체들의 용도.
  6. 원하는 단백질을 코드하는 재조합 DNA를 이용한 제1항 또는 제2항의 효모 돌연변이체의 형질전환, 세포의 발효 및 세로로부터 또는 배양상측액으로부터 원하는 단백질의 분리에 의해 얻을 수 있는 저글리코실화 재조합 단백질.
  7. 제1항 또는 2항의 효모 돌연변이체로부터의 단백질을 코드하는 DNA의 형질변환, 발효 및 세포 또는 배양 상층액으로부터 단백질의 분리에 의해 균일하게 글리코실화된 단백질의 발현 및 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임
KR1019930015252A 1992-08-07 1993-08-06 N-글리코실화에 결함이 있는 효모 숙주 균주 KR0140973B1 (ko)

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DE4226094 1992-08-07
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DEP4301932.3 1993-01-25
DE4301932A DE4301932A1 (de) 1992-08-07 1993-01-25 Hefewirtsstämme mit Defekten in der N-Glycosylierung

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CA (1) CA2103522C (ko)
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Cited By (1)

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KR101460561B1 (ko) * 2012-11-20 2014-11-13 한국식품연구원 사카로마이세스 세레비지애 113-8의 대량 생산방법

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FR2750434B1 (fr) * 1996-06-26 1998-08-21 Applic De Rech Et De Conseils Produit de stabilisation proteique des vins
US6566122B1 (en) 2000-06-16 2003-05-20 Academia Sinica Super-secreting saccharomyces cerevisiae strains
US6803225B2 (en) * 2000-06-30 2004-10-12 Flanders Interuniversity Institute For Biotechnology Protein glycosylation modification in Pichia pastoris
KR100473658B1 (ko) * 2002-10-25 2005-03-10 드림바이오젠 주식회사 위치 선택적 수식법에 의한 혼성형 단백질 치료제 개발을 위한 형질전환 효모 시스템
US7507573B2 (en) 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
JP2009509970A (ja) * 2005-09-22 2009-03-12 プロサイ インコーポレイテッド 酵母突然変異体において産生されるグリコシル化ポリペプチドおよびその使用方法
JP6991126B2 (ja) * 2016-03-30 2022-01-12 株式会社カネカ エンドヌクレアーゼ活性を有するポリペプチド及びその製造方法
CN107988177B (zh) * 2016-10-26 2021-08-20 潍坊康地恩生物科技有限公司 葡萄糖氧化酶突变体
CN108118037B (zh) * 2016-11-28 2021-08-31 青岛蔚蓝生物集团有限公司 一种耐热性提高的葡萄糖氧化酶突变体

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US5135854A (en) * 1987-10-29 1992-08-04 Zymogenetics, Inc. Methods of regulating protein glycosylation
DE3887082T2 (de) * 1987-10-29 1994-04-28 Zymogenetics Inc Verfahren zur Regulierung der Proteinglycosylierung.
NZ229260A (en) * 1988-06-03 1994-02-25 Merck & Co Inc Hepatitis b virus, expression cassette for pre-s domain, host cells and
US5094951A (en) * 1988-06-21 1992-03-10 Chiron Corporation Production of glucose oxidase in recombinant systems
EP0382332A3 (en) * 1989-01-06 1990-11-14 Collaborative Research Inc. Improved supersecreting mutants of saccharomyces cerevisiae
CA2022109A1 (en) * 1989-07-31 1991-02-01 Cheryl A. Schulman Enhanced expression of heterologous proteins in recombinant hosts in a non-growth media
DE4301904A1 (de) * 1992-08-07 1994-02-10 Boehringer Mannheim Gmbh Hypoglycosylierte recombinante Glucoseoxidase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101460561B1 (ko) * 2012-11-20 2014-11-13 한국식품연구원 사카로마이세스 세레비지애 113-8의 대량 생산방법

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FI933487A0 (fi) 1993-08-06
DE4301932A1 (de) 1994-02-10
JP2984229B2 (ja) 1999-11-29
JP2675511B2 (ja) 1997-11-12
AU657230B2 (en) 1995-03-02
IL106589A0 (en) 1993-12-08
FI933487A (fi) 1994-02-08
IL106589A (en) 2005-07-25
US5705616A (en) 1998-01-06
US5798226A (en) 1998-08-25
EP0582244A2 (de) 1994-02-09
NO932811L (no) 1994-02-08
AU4435493A (en) 1994-02-24
ATE207955T1 (de) 2001-11-15
NZ248323A (en) 1994-12-22
CA2103522C (en) 1998-07-07
JPH1052277A (ja) 1998-02-24
DE59310233D1 (de) 2001-12-06
CA2103522A1 (en) 1994-02-08
JPH06296482A (ja) 1994-10-25
EP0582244B1 (de) 2001-10-31
FI110006B (fi) 2002-11-15
EP0582244A3 (de) 1995-03-15
KR0140973B1 (ko) 1998-06-15
NO932811D0 (no) 1993-08-06
NO312246B1 (no) 2002-04-15

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