ES8607391A1 - Procedimiento para producir un fragmento de dna que compren-de un promotor de fosfatasa acida de levadura - Google Patents

Procedimiento para producir un fragmento de dna que compren-de un promotor de fosfatasa acida de levadura

Info

Publication number
ES8607391A1
ES8607391A1 ES540880A ES540880A ES8607391A1 ES 8607391 A1 ES8607391 A1 ES 8607391A1 ES 540880 A ES540880 A ES 540880A ES 540880 A ES540880 A ES 540880A ES 8607391 A1 ES8607391 A1 ES 8607391A1
Authority
ES
Spain
Prior art keywords
yeast
hybrid vectors
production
promoter
polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES540880A
Other languages
English (en)
Other versions
ES540880A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB838315145A external-priority patent/GB8315145D0/en
Application filed by Ciba Geigy AG filed Critical Ciba Geigy AG
Publication of ES8607391A1 publication Critical patent/ES8607391A1/es
Publication of ES540880A0 publication Critical patent/ES540880A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Public Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)

Abstract

PROCEDIMIENTO PARA PRODUCIR UN FRAGMENTO DE DNA QUE COMPRENDE UN PROMOTOR DE FOSFATASA ACIDA DE LEVADURA. SE PROPONE UN GEN DE FOSFATASA ACIDA COMPLEMENTANDO CEPAS DE LEVADURA DEFICIENTES EN FOSFATASA ACIDA POR TRANSFORMACION CON DNA PLASMIDO DE LEVADURA QUECONTIENE ESTE GEN, SE PREPARARAN SUBCLONES DEL GEN OBTENIDO Y SE IDENTIFICA LA UBICACION DE LA REGION PROMOTORA DE LOS SUBCLONES Y SE AISLAN FRAGMENTOSDE DNA QUE CONTENGAN EL PROMOTOR DE FOSFATASA ACIDA, SIENDO ESTE PROMOTOR EL PHO3 O PHO5 O MUTANTES DE LOS MISMOS QUE CONSERVAN LA FUNCION DEL PROMOTOR. ESTOS FRAGMENTOS SE DIGIEREN CON ENDONUCLEASA DE RESTRICCION O EXONUCLEASA PARA ACORTAR LA SECUENCIA LATERAL SIN AFECTAR A LA FUNCION DEL MOTOR. ESTOS FRAGMENTOS SE UNEN A LIGADORES SINTETIZADOS QUIMICAMENTE QUE INCLUYEN LA SECUENCIA DE RECONOCIMIENTO DE LA ENDONUCLEASA DE RESTRICCION Y COMPRENDE UN PUNTO DE RESTRICCION ECORI.
ES540880A 1982-08-09 1985-03-01 Procedimiento para producir un fragmento de dna que compren-de un promotor de fosfatasa acida de levadura Expired ES8607391A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB8222883 1982-08-09
GB8237026 1982-12-31
GB838315145A GB8315145D0 (en) 1982-08-09 1983-06-02 Yeast hybrid vectors

Publications (2)

Publication Number Publication Date
ES8607391A1 true ES8607391A1 (es) 1986-05-16
ES540880A0 ES540880A0 (es) 1986-05-16

Family

ID=27261693

Family Applications (3)

Application Number Title Priority Date Filing Date
ES524816A Granted ES524816A0 (es) 1982-08-09 1983-08-08 Procedimiento para la produccion de polipetidos mediante levadura.
ES540880A Expired ES8607391A1 (es) 1982-08-09 1985-03-01 Procedimiento para producir un fragmento de dna que compren-de un promotor de fosfatasa acida de levadura
ES540881A Expired ES8607026A1 (es) 1982-08-09 1985-03-01 Procedimiento para producir vectores hibridos de levadura

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES524816A Granted ES524816A0 (es) 1982-08-09 1983-08-08 Procedimiento para la produccion de polipetidos mediante levadura.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES540881A Expired ES8607026A1 (es) 1982-08-09 1985-03-01 Procedimiento para producir vectores hibridos de levadura

Country Status (20)

Country Link
EP (1) EP0100561B1 (es)
JP (2) JPH0653073B2 (es)
AT (1) ATE43636T1 (es)
AU (1) AU566348B2 (es)
CA (1) CA1341381C (es)
CY (1) CY1451A (es)
DD (1) DD218118A5 (es)
DE (1) DE3379960D1 (es)
DK (1) DK174905B1 (es)
ES (3) ES524816A0 (es)
FI (1) FI80720C (es)
GB (1) GB2125047B (es)
GR (1) GR78924B (es)
HK (1) HK90688A (es)
HU (1) HU204095B (es)
IE (1) IE55598B1 (es)
NO (1) NO172548C (es)
NZ (1) NZ205178A (es)
PH (1) PH25617A (es)
PT (1) PT77167B (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
EP0103201B1 (en) * 1982-08-16 1990-05-30 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Shuttle vector
JPS5936699A (ja) * 1982-08-20 1984-02-28 Chemo Sero Therapeut Res Inst シヤトルベクタ−
JPS5931799A (ja) * 1982-08-16 1984-02-20 Science & Tech Agency Ｂ型肝炎ウイルス遺伝子を組込んだ組換えプラスミド
AU584580B2 (en) * 1982-09-08 1989-06-01 Smith Kline - Rit Hepatitis B virus vaccine
JPS5974988A (ja) * 1982-10-20 1984-04-27 Suntory Ltd 酵母の誘導発現型プラスミドベクタ−およびその利用方法
ZA843275B (en) * 1983-05-05 1984-12-24 Hoffmann La Roche Novel vectors for interferon expression
DE3479520D1 (en) * 1983-11-21 1989-09-28 Ciba Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast
JPS60248181A (ja) * 1984-05-23 1985-12-07 Shiseido Co Ltd 酵母発現ベクタ−
US5723292A (en) * 1984-05-25 1998-03-03 Zymogenetics, Inc. Stable DNA constructs
AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs
GB8415186D0 (en) * 1984-06-14 1984-07-18 Ciba Geigy Ag Polypeptides
JPS61502655A (ja) * 1984-07-09 1986-11-20 インテグレ−テッド・ジェネティックス・インコ−ポレ−テッド 酵母クロ−ニングビヒクル
JPS61502795A (ja) * 1984-07-09 1986-12-04 レモント，ジェフリ−・エフ 酵母クロ−ニング　ビ−クル
EP0192679A4 (en) * 1984-07-31 1988-04-26 Integrated Genetics Inc YEAST CLONING VECTORS.
JPS61103895A (ja) * 1984-10-26 1986-05-22 Green Cross Corp:The ＨＢｓＡｇの精製方法
US5336609A (en) * 1984-12-06 1994-08-09 Labofina, S.A. Transformed yeasts for producing lysozyme, plasmids used for this transformation, method of producing lysozyme
EP0194006B1 (en) * 1985-02-01 1992-04-29 Imperial Chemical Industries Plc Analogous interferon polypeptides, process for their preparation and pharmaceutical compositions containing them
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
JPS62151183A (ja) * 1985-09-18 1987-07-06 Green Cross Corp:The 酵母プロモ−タ−及び異種蛋白質の製造方法
EP0216573B1 (en) * 1985-09-18 1990-11-22 Green Cross Corporation Yeast promoter and method of producing heterologous proteins
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
GB2188049B (en) * 1986-03-15 1990-09-05 Ciba Geigy Ag Insecticidal proteinaceous substance
EP0240224A3 (en) * 1986-03-31 1989-02-01 Interferon Sciences, Inc. An alpha interferon analogue
US4857467A (en) * 1986-07-23 1989-08-15 Phillips Petroleum Company Carbon and energy source markers for transformation of strains of the genes Pichia
DE3789413T2 (de) 1986-10-03 1994-07-28 Ciba Geigy Ag Lymphokin-ähnliche Peptide.
US5013652A (en) * 1986-10-14 1991-05-07 Genex Corporation Composite yeast vectors
ATE103930T1 (de) * 1988-03-07 1994-04-15 Ciba Geigy Ag Modifizierte proteine.
US5180667A (en) * 1988-03-07 1993-01-19 Ciba-Geigy Corporation Genes encoding eglin C mutants
EP0394409B1 (en) 1988-09-02 1997-06-11 The Rockefeller University Macrophage-derived inflammatory mediator (mip-2)
JPH02145193A (ja) * 1988-11-19 1990-06-04 Lotte Confectionery Co Ltd 遺伝子の組換えによる酵母でのヒト・アルファ・インターフェロンの製造方法
AU648214B2 (en) * 1991-12-31 1994-04-14 Lucky Limited Recombinant gene coding for human alpha interferon and expression vector thereof, etc.
JPH08508640A (ja) 1993-03-10 1996-09-17 スミスクライン・ビーチャム・コーポレイション ヒト脳ホスホジエステラーゼ
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ATE492631T1 (de) 2002-09-13 2011-01-15 Cornell Res Foundation Inc Verwendung von mutationen zur verbesserung von aspergillus-phytasen
DE102005030552B4 (de) * 2005-06-22 2009-05-28 Eucodis Bioscience Selektion von Phosphatase-Aktivität
CN102059495A (zh) * 2010-11-06 2011-05-18 中国长江航运集团宜昌船厂 一种船体框架胎模及加工方法
CN107090441A (zh) 2010-12-09 2017-08-25 巴斯德研究所 用于获得高产量重组蛋白表达的基于mgmt的方法
WO2013059507A1 (en) 2011-10-20 2013-04-25 University Of Iowa Research Foundation N-demethyase genes and uses thereof
JP6215223B2 (ja) 2011-12-09 2017-10-18 アンスティテュ・パストゥール マルチプレックス免疫スクリーニングアッセイ
WO2013187957A1 (en) 2012-06-15 2013-12-19 University Of Iowa Research Foundation Caffeine responsive promoters and regulatory genes
WO2022049409A1 (en) 2020-09-02 2022-03-10 Sia Terragen Express diagnosticum for sars-cov-2

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
FR2458585A1 (fr) * 1979-06-07 1981-01-02 Pasteur Institut Procede de production de proteines par expression des genes correspondants dans des micro-organismes et vecteurs susceptibles d'etre mis en oeuvre dans de tels procedes
AU546577B2 (en) * 1980-02-25 1985-09-05 Trustees Of Columbia University, The Use of eucaryotic promoter sequences in the production of proteinaceous materials
IL63270A0 (en) * 1980-08-05 1981-10-30 Univ Leland Stanford Junior Eukaryotic autonomously replicating segment
IL63035A (en) * 1980-09-09 1985-05-31 Univ California Dnas capable of replication and stable mitotic maintenance in a host eukaryote,their preparation and eukaryotic cells comprising them
NZ199722A (en) * 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
DK368882A (da) * 1981-08-25 1983-02-26 Alan John Kingsman Expessions vektorer
NZ201705A (en) * 1981-08-31 1986-03-14 Genentech Inc Recombinant dna method for production of hepatitis b surface antigen in yeast
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
JPS5931799A (ja) * 1982-08-16 1984-02-20 Science & Tech Agency Ｂ型肝炎ウイルス遺伝子を組込んだ組換えプラスミド

Also Published As

Publication number Publication date
JPH0515379A (ja) 1993-01-26
IE831860L (en) 1984-02-09
DD218118A5 (de) 1985-01-30
FI80720B (fi) 1990-03-30
ES8505724A1 (es) 1985-06-01
GB2125047A (en) 1984-02-29
PH25617A (en) 1991-08-08
CA1341381C (en) 2002-08-06
EP0100561B1 (en) 1989-05-31
EP0100561A1 (en) 1984-02-15
NO832848L (no) 1984-02-10
GR78924B (es) 1984-10-02
ES540881A0 (es) 1986-05-16
ATE43636T1 (de) 1989-06-15
NZ205178A (en) 1986-09-10
FI832825A0 (fi) 1983-08-05
PT77167A (en) 1983-09-01
JPH0653073B2 (ja) 1994-07-20
DE3379960D1 (en) 1989-07-06
FI832825A (fi) 1984-02-10
AU566348B2 (en) 1987-10-15
CY1451A (en) 1989-03-10
HK90688A (en) 1988-11-18
DK361483D0 (da) 1983-08-08
ES540880A0 (es) 1986-05-16
GB8319099D0 (en) 1983-08-17
JPS5948082A (ja) 1984-03-19
DK174905B1 (da) 2004-02-16
GB2125047B (en) 1986-02-19
DK361483A (da) 1984-02-10
ES8607026A1 (es) 1986-05-16
NO172548B (no) 1993-04-26
HU204095B (en) 1991-11-28
IE55598B1 (en) 1990-11-21
NO172548C (no) 1993-08-04
ES524816A0 (es) 1985-06-01
FI80720C (fi) 1990-07-10
PT77167B (en) 1986-03-20
AU1768283A (en) 1984-02-16
JPH0813277B2 (ja) 1996-02-14

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Legal Events

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PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20050713