GB2188049B - Insecticidal proteinaceous substance - Google Patents

Insecticidal proteinaceous substance

Info

Publication number
GB2188049B
GB2188049B GB8606441A GB8606441A GB2188049B GB 2188049 B GB2188049 B GB 2188049B GB 8606441 A GB8606441 A GB 8606441A GB 8606441 A GB8606441 A GB 8606441A GB 2188049 B GB2188049 B GB 2188049B
Authority
GB
United Kingdom
Prior art keywords
proteinaceous substance
insecticidal
coding
dna
dna fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
GB8606441A
Other versions
GB2188049A (en
Inventor
Martin Geiser
Albert Hinnen
Jakob Brassel
Silvia Schweitzer-Gruetzmacher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy AG filed Critical Ciba Geigy AG
Priority to GB8606441A priority Critical patent/GB2188049B/en
Priority to BG080405A priority patent/BG46752A3/en
Priority to AT87810135T priority patent/ATE97163T1/en
Priority to EP87810135A priority patent/EP0238441B1/en
Priority to ES87810135T priority patent/ES2059404T3/en
Priority to DE87810135T priority patent/DE3788077D1/en
Priority to FI871070A priority patent/FI871070A/en
Priority to EG14887A priority patent/EG18869A/en
Priority to NO871062A priority patent/NO871062L/en
Priority to PT84474A priority patent/PT84474B/en
Priority to AU69992/87A priority patent/AU608508B2/en
Priority to PL1987264628A priority patent/PL154252B1/en
Priority to DK128687A priority patent/DK128687A/en
Priority to DD87316204A priority patent/DD297763A5/en
Priority to MA21140A priority patent/MA20900A1/en
Priority to BR8701162A priority patent/BR8701162A/en
Priority to DD30077687A priority patent/DD259420A5/en
Priority to HU871122A priority patent/HU204092B/en
Priority to NZ219611A priority patent/NZ219611A/en
Priority to ZA871830A priority patent/ZA871830B/en
Priority to IL81886A priority patent/IL81886A/en
Priority to IE66587A priority patent/IE59456B1/en
Priority to BG078876A priority patent/BG46006A3/en
Priority to KR1019870002292A priority patent/KR900007640B1/en
Priority to TR163/87A priority patent/TR23521A/en
Priority to JP62060879A priority patent/JPS62224295A/en
Priority to TNTNSN87038A priority patent/TNSN87038A1/en
Publication of GB2188049A publication Critical patent/GB2188049A/en
Application granted granted Critical
Publication of GB2188049B publication Critical patent/GB2188049B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

The present invention is directed to a method for producing an insecticidal proteinaceous substance including the discovery and identification of the entire DNA sequence coding for the insecticidal protein MGE 1 of said proteinaceous substance, to said proteinaceous substance, to a DNA fragment characterized by the nucleotide sequence given in table 2, said fragment coding for the protein MGE 1, to the protein MGE 1 itself, to a DNA fragment originating from Bacillus thuringiensis var.kurstaki from Hpal (O) to Pstl (4355) coding for an insecticidal proteinaceous substance including truncated portions thereof, said truncated DNA portions being subject to the proviso that insecticidal activity has not been lost, to the method of constructing cloning vehicles and expression vehicles comprising the DNA fragment given in table 2 and also to said vehicles themselves.
GB8606441A 1986-03-15 1986-03-15 Insecticidal proteinaceous substance Expired - Lifetime GB2188049B (en)

Priority Applications (27)

Application Number Priority Date Filing Date Title
GB8606441A GB2188049B (en) 1986-03-15 1986-03-15 Insecticidal proteinaceous substance
BG080405A BG46752A3 (en) 1986-03-15 1987-03-03 Insecticide means and method for protection from insecticides
AT87810135T ATE97163T1 (en) 1986-03-15 1987-03-09 INSECTICIDICALLY ACTIVE PROTEIN SUBSTANCE.
EP87810135A EP0238441B1 (en) 1986-03-15 1987-03-09 Insecticidal proteinaceous substance
ES87810135T ES2059404T3 (en) 1986-03-15 1987-03-09 INSECTICIDE PROTEIN SUBSTANCE.
DE87810135T DE3788077D1 (en) 1986-03-15 1987-03-09 Insecticidal proteinaceous substance.
FI871070A FI871070A (en) 1986-03-15 1987-03-11 INSEKTICID PROTEINHALTIG SUBSTANS.
EG14887A EG18869A (en) 1986-03-15 1987-03-12 Insecticidal proteinaceous substance
ZA871830A ZA871830B (en) 1986-03-15 1987-03-13 Insecticidal proteinaceous substance
AU69992/87A AU608508B2 (en) 1986-03-15 1987-03-13 Insecticidal proteinaceous substance
PL1987264628A PL154252B1 (en) 1986-03-15 1987-03-13 Insecticide
DK128687A DK128687A (en) 1986-03-15 1987-03-13 PROTEINS AND THEIR PREPARATION AND USE
DD87316204A DD297763A5 (en) 1986-03-15 1987-03-13 INSECTICIDES AND METHODS OF CONTROLLING INSECTS
MA21140A MA20900A1 (en) 1986-03-15 1987-03-13 Insecticidal proteinaceous substance and process for its preparation
NO871062A NO871062L (en) 1986-03-15 1987-03-13 Insecticides.
DD30077687A DD259420A5 (en) 1986-03-15 1987-03-13 Process for the preparation of a DNA fragment from Bacillus thuringiensis var.kurstaki
HU871122A HU204092B (en) 1986-03-15 1987-03-13 Process for producing dna fragmentim encoding protein-like insecticidal compound and compositions comprising the insecticidal compounds
NZ219611A NZ219611A (en) 1986-03-15 1987-03-13 Insecticidal peptide produced by recombinant dna techniques
PT84474A PT84474B (en) 1986-03-15 1987-03-13 PROCESS FOR THE PREPARATION OF AN INSECTICIDE PROTEIN SUBSTANCE
IL81886A IL81886A (en) 1986-03-15 1987-03-13 Bacillus thuringiensis insecticidal proteinaceous substance,dna encoding it,its preparation and its use
IE66587A IE59456B1 (en) 1986-03-15 1987-03-13 Insecticidal proteinaceous substance
BG078876A BG46006A3 (en) 1986-03-15 1987-03-13 Method for preparing insecticide proteic substance
BR8701162A BR8701162A (en) 1986-03-15 1987-03-13 DNA FRAGMENT, PROTEINACEA SUBSTANCE, PROTEIN, VECTOR, MICROORGANISM, BIOENCAPSULATION SYSTEM, METHOD TO PRODUCE A DNA FRAGMENT, METHOD TO COMBAT INSECTS, INSECTICID COMPOSITION, HYBRID HYBRID, COMPOUND HYBRID
KR1019870002292A KR900007640B1 (en) 1986-03-15 1987-03-14 Process form producing of insecticidal proteinaceous substance
TR163/87A TR23521A (en) 1986-03-15 1987-03-16 PROTEINED SUBSTANCE
JP62060879A JPS62224295A (en) 1986-03-15 1987-03-16 Insecticidal protein substance
TNTNSN87038A TNSN87038A1 (en) 1986-03-15 1987-03-16 INSECTICIDE PROTEIN SUBSTANCE AND PROCESS FOR THE PREPARATION THEREOF

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8606441A GB2188049B (en) 1986-03-15 1986-03-15 Insecticidal proteinaceous substance

Publications (2)

Publication Number Publication Date
GB2188049A GB2188049A (en) 1987-09-23
GB2188049B true GB2188049B (en) 1990-09-05

Family

ID=10594681

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8606441A Expired - Lifetime GB2188049B (en) 1986-03-15 1986-03-15 Insecticidal proteinaceous substance

Country Status (25)

Country Link
EP (1) EP0238441B1 (en)
JP (1) JPS62224295A (en)
KR (1) KR900007640B1 (en)
AT (1) ATE97163T1 (en)
AU (1) AU608508B2 (en)
BG (2) BG46752A3 (en)
BR (1) BR8701162A (en)
DD (2) DD259420A5 (en)
DE (1) DE3788077D1 (en)
DK (1) DK128687A (en)
EG (1) EG18869A (en)
ES (1) ES2059404T3 (en)
FI (1) FI871070A (en)
GB (1) GB2188049B (en)
HU (1) HU204092B (en)
IE (1) IE59456B1 (en)
IL (1) IL81886A (en)
MA (1) MA20900A1 (en)
NO (1) NO871062L (en)
NZ (1) NZ219611A (en)
PL (1) PL154252B1 (en)
PT (1) PT84474B (en)
TN (1) TNSN87038A1 (en)
TR (1) TR23521A (en)
ZA (1) ZA871830B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317511A3 (en) * 1987-11-18 1991-10-16 Ciba-Geigy Ag Insecticidal cotton plant cells
CA2101610A1 (en) * 1992-08-07 1994-02-08 William D. Prevatt Production of bacillus entomotoxins in methylotrophic yeast
GB9618083D0 (en) * 1996-08-29 1996-10-09 Mini Agriculture & Fisheries Pesticidal agents

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2093860A (en) * 1981-02-27 1982-09-08 Shionogi Seiyaku Kk Insecticidal substances and their production from b thuringiensis mutants
EP0063949A2 (en) * 1981-04-27 1982-11-03 Washington Research Foundation Plasmids coding for B. Thuringiensis crystal protein, their production and their use in creating genetically engineered bacterial strains, the aforesaid strains per se and their use in producing said protein, said protein so-produced, insecticidal formulations comprising the same and a method of producing an insecticidal effect
EP0093062A1 (en) * 1982-04-26 1983-11-02 Institut Pasteur DNA containing a sequence encoding for a crystal protein or a polypeptide having insecticidal properties, microorganisms transformed with such DNA, compositions containing said crystal proteins, polypeptide or microorganisms
WO1986001536A1 (en) * 1984-08-31 1986-03-13 Washington Research Foundation Bacillus thuringiensis crystal protein gene toxin segment
GB2165261A (en) * 1984-10-09 1986-04-09 Agricultural Genetics Co Bacillus thuringiensis
EP0185005A2 (en) * 1984-12-10 1986-06-18 Monsanto Company Insertion of the bacillus thuringiensis crystal protein gene into plant-colonizing microorganisms and their use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
CA1341283C (en) * 1985-03-28 2001-08-14 Frank H. Gaertner Biological pesticides and methods for their delivery and use
JPS62100292A (en) * 1985-10-28 1987-05-09 Sumitomo Chem Co Ltd Insecticidal protein gene of bacillus thuringensis aizawai ipl strain
CA1341092C (en) * 1985-12-12 2000-09-05 David L. Edwards Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2093860A (en) * 1981-02-27 1982-09-08 Shionogi Seiyaku Kk Insecticidal substances and their production from b thuringiensis mutants
EP0059460A2 (en) * 1981-02-27 1982-09-08 Shionogi & Co., Ltd. An insecticide and a process for its preparation
EP0063949A2 (en) * 1981-04-27 1982-11-03 Washington Research Foundation Plasmids coding for B. Thuringiensis crystal protein, their production and their use in creating genetically engineered bacterial strains, the aforesaid strains per se and their use in producing said protein, said protein so-produced, insecticidal formulations comprising the same and a method of producing an insecticidal effect
EP0093062A1 (en) * 1982-04-26 1983-11-02 Institut Pasteur DNA containing a sequence encoding for a crystal protein or a polypeptide having insecticidal properties, microorganisms transformed with such DNA, compositions containing said crystal proteins, polypeptide or microorganisms
WO1986001536A1 (en) * 1984-08-31 1986-03-13 Washington Research Foundation Bacillus thuringiensis crystal protein gene toxin segment
GB2165261A (en) * 1984-10-09 1986-04-09 Agricultural Genetics Co Bacillus thuringiensis
EP0185005A2 (en) * 1984-12-10 1986-06-18 Monsanto Company Insertion of the bacillus thuringiensis crystal protein gene into plant-colonizing microorganisms and their use

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Appl. Environ. Microbiol. (1985) Vol.50 No.3 pp623-628 *
Han Guk J. Genet. Eng. (1984) Vol.1 No.1 pp29-35 *
J. Bacteriol. (1986) Vol.166 No.3 pp801-811 *
J. Invert. Path. (1979) Vol.33 No.2 pp233-238 *
Proc. Natl. Acad. Sci. (1982) Vol.79 No.19 pp6065-6069 *
Proc. Natl. Acad. Sci. (1982) Vol.79 No.22 pp6951-6955 *

Also Published As

Publication number Publication date
IE59456B1 (en) 1994-02-23
AU608508B2 (en) 1991-04-11
DE3788077D1 (en) 1993-12-16
DD297763A5 (en) 1992-01-23
PL154252B1 (en) 1991-07-31
HU204092B (en) 1991-11-28
KR900007640B1 (en) 1990-10-17
FI871070A0 (en) 1987-03-11
DK128687A (en) 1987-09-16
FI871070A (en) 1987-09-16
TNSN87038A1 (en) 1990-01-01
AU6999287A (en) 1987-09-17
ES2059404T3 (en) 1994-11-16
BR8701162A (en) 1988-01-12
ZA871830B (en) 1987-11-25
EP0238441A3 (en) 1988-08-10
PT84474A (en) 1987-04-01
IE870665L (en) 1987-09-15
DK128687D0 (en) 1987-03-13
NZ219611A (en) 1991-01-29
EP0238441B1 (en) 1993-11-10
PT84474B (en) 1989-11-10
TR23521A (en) 1990-03-01
JPS62224295A (en) 1987-10-02
DD259420A5 (en) 1988-08-24
EG18869A (en) 1994-02-28
IL81886A0 (en) 1987-10-20
KR870009016A (en) 1987-10-22
IL81886A (en) 1992-02-16
PL264628A1 (en) 1988-04-28
ATE97163T1 (en) 1993-11-15
HUT43628A (en) 1987-11-30
MA20900A1 (en) 1987-10-01
NO871062L (en) 1987-09-16
EP0238441A2 (en) 1987-09-23
BG46006A3 (en) 1989-09-15
BG46752A3 (en) 1990-02-15
NO871062D0 (en) 1987-03-13
GB2188049A (en) 1987-09-23

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PE20 Patent expired after termination of 20 years

Effective date: 20060314