IL81886A - Bacillus thuringiensis insecticidal proteinaceous substance,dna encoding it,its preparation and its use - Google Patents

Bacillus thuringiensis insecticidal proteinaceous substance,dna encoding it,its preparation and its use

Info

Publication number
IL81886A
IL81886A IL81886A IL8188687A IL81886A IL 81886 A IL81886 A IL 81886A IL 81886 A IL81886 A IL 81886A IL 8188687 A IL8188687 A IL 8188687A IL 81886 A IL81886 A IL 81886A
Authority
IL
Israel
Prior art keywords
proteinaceous substance
insecticidal
bacillus thuringiensis
coding
dna
Prior art date
Application number
IL81886A
Other versions
IL81886A0 (en
Original Assignee
Ciba Geigy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy filed Critical Ciba Geigy
Publication of IL81886A0 publication Critical patent/IL81886A0/en
Publication of IL81886A publication Critical patent/IL81886A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

The present invention is directed to a method for producing an insecticidal proteinaceous substance including the discovery and identification of the entire DNA sequence coding for the insecticidal protein MGE 1 of said proteinaceous substance, to said proteinaceous substance, to a DNA fragment characterized by the nucleotide sequence given in table 2, said fragment coding for the protein MGE 1, to the protein MGE 1 itself, to a DNA fragment originating from Bacillus thuringiensis var.kurstaki from Hpal (O) to Pstl (4355) coding for an insecticidal proteinaceous substance including truncated portions thereof, said truncated DNA portions being subject to the proviso that insecticidal activity has not been lost, to the method of constructing cloning vehicles and expression vehicles comprising the DNA fragment given in table 2 and also to said vehicles themselves.
IL81886A 1986-03-15 1987-03-13 Bacillus thuringiensis insecticidal proteinaceous substance,dna encoding it,its preparation and its use IL81886A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8606441A GB2188049B (en) 1986-03-15 1986-03-15 Insecticidal proteinaceous substance

Publications (2)

Publication Number Publication Date
IL81886A0 IL81886A0 (en) 1987-10-20
IL81886A true IL81886A (en) 1992-02-16

Family

ID=10594681

Family Applications (1)

Application Number Title Priority Date Filing Date
IL81886A IL81886A (en) 1986-03-15 1987-03-13 Bacillus thuringiensis insecticidal proteinaceous substance,dna encoding it,its preparation and its use

Country Status (25)

Country Link
EP (1) EP0238441B1 (en)
JP (1) JPS62224295A (en)
KR (1) KR900007640B1 (en)
AT (1) ATE97163T1 (en)
AU (1) AU608508B2 (en)
BG (2) BG46752A3 (en)
BR (1) BR8701162A (en)
DD (2) DD259420A5 (en)
DE (1) DE3788077D1 (en)
DK (1) DK128687A (en)
EG (1) EG18869A (en)
ES (1) ES2059404T3 (en)
FI (1) FI871070A (en)
GB (1) GB2188049B (en)
HU (1) HU204092B (en)
IE (1) IE59456B1 (en)
IL (1) IL81886A (en)
MA (1) MA20900A1 (en)
NO (1) NO871062L (en)
NZ (1) NZ219611A (en)
PL (1) PL154252B1 (en)
PT (1) PT84474B (en)
TN (1) TNSN87038A1 (en)
TR (1) TR23521A (en)
ZA (1) ZA871830B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317511A3 (en) * 1987-11-18 1991-10-16 Ciba-Geigy Ag Insecticidal cotton plant cells
CA2101610A1 (en) * 1992-08-07 1994-02-08 William D. Prevatt Production of bacillus entomotoxins in methylotrophic yeast
GB9618083D0 (en) * 1996-08-29 1996-10-09 Mini Agriculture & Fisheries Pesticidal agents

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57142907A (en) * 1981-02-27 1982-09-03 Shionogi & Co Ltd Bacterium-originated insecticide and its preparation
JPH0795953B2 (en) * 1981-04-27 1995-10-18 ワシントン・リサ−チ・ファンデ−ション Bacillus thuringiensis microcrystalline protein in Escherichia coli
FR2525630B1 (en) * 1982-04-26 1985-07-05 Pasteur Institut DNA CONTAINING A SEQUENCE ENCODING A CRYSTAL PROTEIN OR A POLYPEPTIDE HAVING INSECTICIDE PROPERTIES, MICROORGANISMS TRANSFORMED BY SUCH DNA, COMPOSITIONS CONTAINING SAID CRYSTAL PROTEINS, POLYPEPTIDE OR MICROORGANISMS
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
CA1301094C (en) * 1984-08-31 1992-05-19 Helen Riaboff Whiteley Bacillus thuringiensis crystal protein gene toxin segment
GB8425487D0 (en) * 1984-10-09 1984-11-14 Agricultural Genetics Co Strain of bacillus thuringiensis
ZA859400B (en) * 1984-12-10 1986-10-29 Monsanto Co Insertion of the bacillus thuringiensis crystal protein gene into plant-colonizing microorganisms and their use
CA1341283C (en) * 1985-03-28 2001-08-14 Frank H. Gaertner Biological pesticides and methods for their delivery and use
JPS62100292A (en) * 1985-10-28 1987-05-09 Sumitomo Chem Co Ltd Insecticidal protein gene of bacillus thuringensis aizawai ipl strain
CA1341092C (en) * 1985-12-12 2000-09-05 David L. Edwards Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby

Also Published As

Publication number Publication date
BR8701162A (en) 1988-01-12
GB2188049B (en) 1990-09-05
FI871070A (en) 1987-09-16
HUT43628A (en) 1987-11-30
AU6999287A (en) 1987-09-17
IE870665L (en) 1987-09-15
FI871070A0 (en) 1987-03-11
DK128687D0 (en) 1987-03-13
KR900007640B1 (en) 1990-10-17
PL264628A1 (en) 1988-04-28
TNSN87038A1 (en) 1990-01-01
DD297763A5 (en) 1992-01-23
IE59456B1 (en) 1994-02-23
KR870009016A (en) 1987-10-22
EP0238441A3 (en) 1988-08-10
MA20900A1 (en) 1987-10-01
ZA871830B (en) 1987-11-25
DD259420A5 (en) 1988-08-24
NO871062D0 (en) 1987-03-13
TR23521A (en) 1990-03-01
AU608508B2 (en) 1991-04-11
EG18869A (en) 1994-02-28
DK128687A (en) 1987-09-16
HU204092B (en) 1991-11-28
GB2188049A (en) 1987-09-23
EP0238441A2 (en) 1987-09-23
ES2059404T3 (en) 1994-11-16
NZ219611A (en) 1991-01-29
NO871062L (en) 1987-09-16
ATE97163T1 (en) 1993-11-15
BG46006A3 (en) 1989-09-15
EP0238441B1 (en) 1993-11-10
DE3788077D1 (en) 1993-12-16
PT84474B (en) 1989-11-10
IL81886A0 (en) 1987-10-20
PT84474A (en) 1987-04-01
JPS62224295A (en) 1987-10-02
BG46752A3 (en) 1990-02-15
PL154252B1 (en) 1991-07-31

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Legal Events

Date Code Title Description
KB Patent renewed
HC Change of name of proprietor(s)
KB Patent renewed
KB Patent renewed
EXP Patent expired