ES2831030T3 - Célula madre pluripotente que puede aislarse de tejido corporal - Google Patents
Célula madre pluripotente que puede aislarse de tejido corporal Download PDFInfo
- Publication number
- ES2831030T3 ES2831030T3 ES17163323T ES17163323T ES2831030T3 ES 2831030 T3 ES2831030 T3 ES 2831030T3 ES 17163323 T ES17163323 T ES 17163323T ES 17163323 T ES17163323 T ES 17163323T ES 2831030 T3 ES2831030 T3 ES 2831030T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- cell
- muse
- pluripotent stem
- fractions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000001778 pluripotent stem cell Anatomy 0.000 title claims abstract description 168
- 210000004027 cell Anatomy 0.000 claims abstract description 875
- 238000000034 method Methods 0.000 claims abstract description 37
- 210000002966 serum Anatomy 0.000 claims abstract description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000001301 oxygen Substances 0.000 claims abstract description 8
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 8
- 230000035939 shock Effects 0.000 claims abstract description 6
- 230000000638 stimulation Effects 0.000 claims abstract description 6
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 3
- 235000003642 hunger Nutrition 0.000 claims abstract description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 3
- 239000010452 phosphate Substances 0.000 claims abstract description 3
- 230000037351 starvation Effects 0.000 claims abstract description 3
- 231100000167 toxic agent Toxicity 0.000 claims abstract description 3
- 239000003440 toxic substance Substances 0.000 claims abstract description 3
- 230000005855 radiation Effects 0.000 claims abstract 2
- 210000001519 tissue Anatomy 0.000 claims description 129
- 210000001185 bone marrow Anatomy 0.000 claims description 39
- 210000003491 skin Anatomy 0.000 claims description 17
- 210000004369 blood Anatomy 0.000 claims description 11
- 239000008280 blood Substances 0.000 claims description 11
- 210000000577 adipose tissue Anatomy 0.000 claims description 4
- 210000003954 umbilical cord Anatomy 0.000 claims description 3
- 229940028444 muse Drugs 0.000 description 244
- 210000002950 fibroblast Anatomy 0.000 description 93
- 230000014509 gene expression Effects 0.000 description 78
- GMVPRGQOIOIIMI-DWKJAMRDSA-N prostaglandin E1 Chemical compound CCCCC[C@H](O)\C=C\[C@H]1[C@H](O)CC(=O)[C@@H]1CCCCCCC(O)=O GMVPRGQOIOIIMI-DWKJAMRDSA-N 0.000 description 77
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 72
- 108090000623 proteins and genes Proteins 0.000 description 48
- 108090000631 Trypsin Proteins 0.000 description 41
- 102000004142 Trypsin Human genes 0.000 description 41
- 230000004069 differentiation Effects 0.000 description 41
- 239000012588 trypsin Substances 0.000 description 41
- 229920000609 methyl cellulose Polymers 0.000 description 38
- 239000001923 methylcellulose Substances 0.000 description 38
- 235000010981 methylcellulose Nutrition 0.000 description 38
- 210000002242 embryoid body Anatomy 0.000 description 36
- 238000011534 incubation Methods 0.000 description 32
- 230000015572 biosynthetic process Effects 0.000 description 31
- 230000006698 induction Effects 0.000 description 29
- 241000699670 Mus sp. Species 0.000 description 27
- 239000002609 medium Substances 0.000 description 27
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 description 26
- 101150086694 SLC22A3 gene Proteins 0.000 description 26
- 210000001550 testis Anatomy 0.000 description 26
- 238000004114 suspension culture Methods 0.000 description 24
- 239000003550 marker Substances 0.000 description 22
- 210000005087 mononuclear cell Anatomy 0.000 description 22
- 210000000130 stem cell Anatomy 0.000 description 21
- 238000012258 culturing Methods 0.000 description 20
- 230000012010 growth Effects 0.000 description 20
- 210000000056 organ Anatomy 0.000 description 20
- 241000699666 Mus <mouse, genus> Species 0.000 description 19
- 230000000694 effects Effects 0.000 description 19
- 206010043276 Teratoma Diseases 0.000 description 18
- 230000002500 effect on skin Effects 0.000 description 18
- 238000003757 reverse transcription PCR Methods 0.000 description 18
- 102100037241 Endoglin Human genes 0.000 description 17
- 101000881679 Homo sapiens Endoglin Proteins 0.000 description 17
- 108010017842 Telomerase Proteins 0.000 description 17
- 238000010186 staining Methods 0.000 description 16
- 238000012360 testing method Methods 0.000 description 16
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 15
- 239000003814 drug Substances 0.000 description 15
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 14
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 14
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 14
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 14
- 239000005090 green fluorescent protein Substances 0.000 description 14
- 210000004185 liver Anatomy 0.000 description 14
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 13
- 238000000338 in vitro Methods 0.000 description 13
- 238000003753 real-time PCR Methods 0.000 description 13
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 12
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 12
- -1 Snail1 Proteins 0.000 description 12
- 102000013529 alpha-Fetoproteins Human genes 0.000 description 12
- 108010047303 von Willebrand Factor Proteins 0.000 description 12
- 102100036537 von Willebrand factor Human genes 0.000 description 12
- 229960001134 von willebrand factor Drugs 0.000 description 12
- 101000958041 Homo sapiens Musculin Proteins 0.000 description 11
- 108010020004 Microtubule-Associated Proteins Proteins 0.000 description 11
- 102000009664 Microtubule-Associated Proteins Human genes 0.000 description 11
- 102100033725 Tumor necrosis factor receptor superfamily member 16 Human genes 0.000 description 11
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 11
- 102000046949 human MSC Human genes 0.000 description 11
- 238000001727 in vivo Methods 0.000 description 11
- 210000003205 muscle Anatomy 0.000 description 11
- 230000008929 regeneration Effects 0.000 description 11
- 238000011069 regeneration method Methods 0.000 description 11
- 102000009410 Chemokine receptor Human genes 0.000 description 10
- 108050000299 Chemokine receptor Proteins 0.000 description 10
- 108010010803 Gelatin Proteins 0.000 description 10
- 239000004365 Protease Substances 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
- 239000008273 gelatin Substances 0.000 description 10
- 229920000159 gelatin Polymers 0.000 description 10
- 235000019322 gelatine Nutrition 0.000 description 10
- 235000011852 gelatine desserts Nutrition 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 210000005044 neurofilament Anatomy 0.000 description 10
- 230000002035 prolonged effect Effects 0.000 description 10
- 238000002054 transplantation Methods 0.000 description 10
- 101000801254 Homo sapiens Tumor necrosis factor receptor superfamily member 16 Proteins 0.000 description 9
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 9
- 102000035195 Peptidases Human genes 0.000 description 9
- 108091005804 Peptidases Proteins 0.000 description 9
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 9
- 238000004115 adherent culture Methods 0.000 description 9
- 235000019419 proteases Nutrition 0.000 description 9
- 230000035882 stress Effects 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- 102100023126 Cell surface glycoprotein MUC18 Human genes 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 101000623903 Homo sapiens Cell surface glycoprotein MUC18 Proteins 0.000 description 8
- 102000012547 Olfactory receptors Human genes 0.000 description 8
- 108050002069 Olfactory receptors Proteins 0.000 description 8
- 239000000427 antigen Substances 0.000 description 8
- 102000036639 antigens Human genes 0.000 description 8
- 108091007433 antigens Proteins 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 8
- 210000004207 dermis Anatomy 0.000 description 8
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 239000011521 glass Substances 0.000 description 8
- 238000002991 immunohistochemical analysis Methods 0.000 description 8
- 238000002955 isolation Methods 0.000 description 8
- 230000035755 proliferation Effects 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 210000002460 smooth muscle Anatomy 0.000 description 8
- 210000001082 somatic cell Anatomy 0.000 description 8
- 210000000278 spinal cord Anatomy 0.000 description 8
- 238000003260 vortexing Methods 0.000 description 8
- 102000007469 Actins Human genes 0.000 description 7
- 108010085238 Actins Proteins 0.000 description 7
- 102100028757 Chondroitin sulfate proteoglycan 4 Human genes 0.000 description 7
- 239000012981 Hank's balanced salt solution Substances 0.000 description 7
- 101000916489 Homo sapiens Chondroitin sulfate proteoglycan 4 Proteins 0.000 description 7
- 101001107084 Homo sapiens E3 ubiquitin-protein ligase RNF5 Proteins 0.000 description 7
- 101000819088 Homo sapiens Transcription factor GATA-6 Proteins 0.000 description 7
- 102000008763 Neurofilament Proteins Human genes 0.000 description 7
- 108010088373 Neurofilament Proteins Proteins 0.000 description 7
- 101150114527 Nkx2-5 gene Proteins 0.000 description 7
- 102100021382 Transcription factor GATA-6 Human genes 0.000 description 7
- 101100460507 Xenopus laevis nkx-2.5 gene Proteins 0.000 description 7
- 210000001789 adipocyte Anatomy 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 210000001654 germ layer Anatomy 0.000 description 7
- 238000003306 harvesting Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 210000004940 nucleus Anatomy 0.000 description 7
- 229920002338 polyhydroxyethylmethacrylate Polymers 0.000 description 7
- 230000001105 regulatory effect Effects 0.000 description 7
- 230000008672 reprogramming Effects 0.000 description 7
- 102000008100 Human Serum Albumin Human genes 0.000 description 6
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 6
- 238000010240 RT-PCR analysis Methods 0.000 description 6
- 230000032823 cell division Effects 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 210000002257 embryonic structure Anatomy 0.000 description 6
- 230000002068 genetic effect Effects 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 210000003866 melanoblast Anatomy 0.000 description 6
- 239000013642 negative control Substances 0.000 description 6
- 238000002560 therapeutic procedure Methods 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 5
- 238000000018 DNA microarray Methods 0.000 description 5
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 5
- 108700021430 Kruppel-Like Factor 4 Proteins 0.000 description 5
- 101100154912 Mus musculus Tyrp1 gene Proteins 0.000 description 5
- 101710150336 Protein Rex Proteins 0.000 description 5
- 102100023710 Proteinase-activated receptor 4 Human genes 0.000 description 5
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 5
- 230000024245 cell differentiation Effects 0.000 description 5
- 210000003494 hepatocyte Anatomy 0.000 description 5
- 101150077014 sox10 gene Proteins 0.000 description 5
- 101150033839 4 gene Proteins 0.000 description 4
- 102000018704 Chitinase-3-Like Protein 1 Human genes 0.000 description 4
- 108010066813 Chitinase-3-Like Protein 1 Proteins 0.000 description 4
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 101000613565 Homo sapiens PRKC apoptosis WT1 regulator protein Proteins 0.000 description 4
- 101001113471 Homo sapiens Proteinase-activated receptor 4 Proteins 0.000 description 4
- 108091006905 Human Serum Albumin Proteins 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 229930040373 Paraformaldehyde Natural products 0.000 description 4
- 239000012503 blood component Substances 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 210000000805 cytoplasm Anatomy 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 238000001493 electron microscopy Methods 0.000 description 4
- 210000000981 epithelium Anatomy 0.000 description 4
- 210000002288 golgi apparatus Anatomy 0.000 description 4
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 4
- 238000010253 intravenous injection Methods 0.000 description 4
- 238000002826 magnetic-activated cell sorting Methods 0.000 description 4
- 230000011987 methylation Effects 0.000 description 4
- 238000007069 methylation reaction Methods 0.000 description 4
- 230000001537 neural effect Effects 0.000 description 4
- 210000002569 neuron Anatomy 0.000 description 4
- 210000004409 osteocyte Anatomy 0.000 description 4
- 229920002866 paraformaldehyde Polymers 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 210000004786 perivascular cell Anatomy 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 210000004927 skin cell Anatomy 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 4
- 230000017423 tissue regeneration Effects 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 102100025905 C-Jun-amino-terminal kinase-interacting protein 4 Human genes 0.000 description 3
- 101710105201 C-Jun-amino-terminal kinase-interacting protein 4 Proteins 0.000 description 3
- 102100032912 CD44 antigen Human genes 0.000 description 3
- 108010078791 Carrier Proteins Proteins 0.000 description 3
- 101710151400 Chitinase 3 Proteins 0.000 description 3
- 101710107426 Endochitinase 3 Proteins 0.000 description 3
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 3
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 3
- 102000003969 Fibroblast growth factor 4 Human genes 0.000 description 3
- 108090000381 Fibroblast growth factor 4 Proteins 0.000 description 3
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 3
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 3
- 101000827785 Homo sapiens Alpha-fetoprotein Proteins 0.000 description 3
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 3
- 101000599852 Homo sapiens Intercellular adhesion molecule 1 Proteins 0.000 description 3
- 101001139134 Homo sapiens Krueppel-like factor 4 Proteins 0.000 description 3
- 101000830411 Homo sapiens Probable ATP-dependent RNA helicase DDX4 Proteins 0.000 description 3
- 101000848653 Homo sapiens Tripartite motif-containing protein 26 Proteins 0.000 description 3
- 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 description 3
- 102100020677 Krueppel-like factor 4 Human genes 0.000 description 3
- 102000000562 Monocarboxylic Acid Transporters Human genes 0.000 description 3
- 101710204259 Monocarboxylic acid transporter Proteins 0.000 description 3
- 102100024770 Probable ATP-dependent RNA helicase DDX4 Human genes 0.000 description 3
- 238000011530 RNeasy Mini Kit Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 101150037203 Sox2 gene Proteins 0.000 description 3
- 108010006785 Taq Polymerase Proteins 0.000 description 3
- 102100026404 Teratocarcinoma-derived growth factor 1 Human genes 0.000 description 3
- 101710185710 Teratocarcinoma-derived growth factor 1 Proteins 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 3
- 230000017047 asymmetric cell division Effects 0.000 description 3
- 210000000625 blastula Anatomy 0.000 description 3
- 210000000988 bone and bone Anatomy 0.000 description 3
- 210000000845 cartilage Anatomy 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 210000004748 cultured cell Anatomy 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 238000000635 electron micrograph Methods 0.000 description 3
- 238000003365 immunocytochemistry Methods 0.000 description 3
- 238000012744 immunostaining Methods 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 230000005012 migration Effects 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 210000003470 mitochondria Anatomy 0.000 description 3
- 230000000242 pagocytic effect Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000002062 proliferating effect Effects 0.000 description 3
- 102000005962 receptors Human genes 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 230000001172 regenerating effect Effects 0.000 description 3
- 230000001177 retroviral effect Effects 0.000 description 3
- 210000002027 skeletal muscle Anatomy 0.000 description 3
- 208000020431 spinal cord injury Diseases 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 230000000381 tumorigenic effect Effects 0.000 description 3
- 230000003827 upregulation Effects 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- 229910000859 α-Fe Inorganic materials 0.000 description 3
- 102100022717 Atypical chemokine receptor 1 Human genes 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102100024210 CD166 antigen Human genes 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 108060005980 Collagenase Proteins 0.000 description 2
- 102000029816 Collagenase Human genes 0.000 description 2
- 208000025962 Crush injury Diseases 0.000 description 2
- 102100033672 Deleted in azoospermia-like Human genes 0.000 description 2
- 102100037126 Developmental pluripotency-associated protein 4 Human genes 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 206010073306 Exposure to radiation Diseases 0.000 description 2
- 108091006027 G proteins Proteins 0.000 description 2
- 241000237858 Gastropoda Species 0.000 description 2
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 description 2
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 101000693913 Homo sapiens Albumin Proteins 0.000 description 2
- 101000678879 Homo sapiens Atypical chemokine receptor 1 Proteins 0.000 description 2
- 101000980840 Homo sapiens CD166 antigen Proteins 0.000 description 2
- 101000871280 Homo sapiens Deleted in azoospermia-like Proteins 0.000 description 2
- 101000881868 Homo sapiens Developmental pluripotency-associated protein 4 Proteins 0.000 description 2
- 101001053946 Homo sapiens Dystrophin Proteins 0.000 description 2
- 101000994365 Homo sapiens Integrin alpha-6 Proteins 0.000 description 2
- 101000594424 Homo sapiens Olfactory receptor 10H2 Proteins 0.000 description 2
- 101000594427 Homo sapiens Olfactory receptor 10H3 Proteins 0.000 description 2
- 101000594426 Homo sapiens Olfactory receptor 10H4 Proteins 0.000 description 2
- 101000594445 Homo sapiens Olfactory receptor 10P1 Proteins 0.000 description 2
- 101001122100 Homo sapiens Olfactory receptor 10T2 Proteins 0.000 description 2
- 101001086429 Homo sapiens Olfactory receptor 1L3 Proteins 0.000 description 2
- 101000982241 Homo sapiens Olfactory receptor 2A7 Proteins 0.000 description 2
- 101000982237 Homo sapiens Olfactory receptor 2B6 Proteins 0.000 description 2
- 101000982235 Homo sapiens Olfactory receptor 2C1 Proteins 0.000 description 2
- 101001121141 Homo sapiens Olfactory receptor 2M2 Proteins 0.000 description 2
- 101001137092 Homo sapiens Olfactory receptor 2T5 Proteins 0.000 description 2
- 101000721068 Homo sapiens Olfactory receptor 4D5 Proteins 0.000 description 2
- 101000614002 Homo sapiens Olfactory receptor 4N4 Proteins 0.000 description 2
- 101000721757 Homo sapiens Olfactory receptor 51E2 Proteins 0.000 description 2
- 101000982753 Homo sapiens Olfactory receptor 52A1 Proteins 0.000 description 2
- 101001138475 Homo sapiens Olfactory receptor 5AP2 Proteins 0.000 description 2
- 101000992258 Homo sapiens Olfactory receptor 5P2 Proteins 0.000 description 2
- 101000598887 Homo sapiens Olfactory receptor 6A2 Proteins 0.000 description 2
- 101001121135 Homo sapiens Olfactory receptor 7D4 Proteins 0.000 description 2
- 101001121132 Homo sapiens Olfactory receptor 7G3 Proteins 0.000 description 2
- 101001094700 Homo sapiens POU domain, class 5, transcription factor 1 Proteins 0.000 description 2
- 101000702384 Homo sapiens Protein sprouty homolog 2 Proteins 0.000 description 2
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 2
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 description 2
- 101000835093 Homo sapiens Transferrin receptor protein 1 Proteins 0.000 description 2
- 102100032816 Integrin alpha-6 Human genes 0.000 description 2
- 102100022297 Integrin alpha-X Human genes 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 102000000380 Matrix Metalloproteinase 1 Human genes 0.000 description 2
- 101100510267 Mus musculus Klf4 gene Proteins 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 101150012532 NANOG gene Proteins 0.000 description 2
- 101000783356 Naja sputatrix Cytotoxin Proteins 0.000 description 2
- 208000005289 Neoplastic Cell Transformation Diseases 0.000 description 2
- 108010032605 Nerve Growth Factor Receptors Proteins 0.000 description 2
- 102000008730 Nestin Human genes 0.000 description 2
- 108010088225 Nestin Proteins 0.000 description 2
- 102100035608 Olfactory receptor 10H2 Human genes 0.000 description 2
- 102100035611 Olfactory receptor 10H3 Human genes 0.000 description 2
- 102100035610 Olfactory receptor 10H4 Human genes 0.000 description 2
- 102100035504 Olfactory receptor 10P1 Human genes 0.000 description 2
- 102100027273 Olfactory receptor 10T2 Human genes 0.000 description 2
- 102100032673 Olfactory receptor 1L3 Human genes 0.000 description 2
- 102100026695 Olfactory receptor 2A7 Human genes 0.000 description 2
- 102100026698 Olfactory receptor 2B6 Human genes 0.000 description 2
- 102100026700 Olfactory receptor 2C1 Human genes 0.000 description 2
- 102100026574 Olfactory receptor 2M2 Human genes 0.000 description 2
- 102100035531 Olfactory receptor 2T5 Human genes 0.000 description 2
- 102100025910 Olfactory receptor 4D5 Human genes 0.000 description 2
- 102100040575 Olfactory receptor 4N4 Human genes 0.000 description 2
- 102100025128 Olfactory receptor 51E2 Human genes 0.000 description 2
- 102100026995 Olfactory receptor 52A1 Human genes 0.000 description 2
- 102100020809 Olfactory receptor 5AP2 Human genes 0.000 description 2
- 102100031861 Olfactory receptor 5P2 Human genes 0.000 description 2
- 102100037776 Olfactory receptor 6A2 Human genes 0.000 description 2
- 102100026600 Olfactory receptor 7D4 Human genes 0.000 description 2
- 102100026603 Olfactory receptor 7G3 Human genes 0.000 description 2
- 102000004067 Osteocalcin Human genes 0.000 description 2
- 108090000573 Osteocalcin Proteins 0.000 description 2
- 102100024894 PR domain zinc finger protein 1 Human genes 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 108010009975 Positive Regulatory Domain I-Binding Factor 1 Proteins 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 2
- 102100030400 Protein sprouty homolog 2 Human genes 0.000 description 2
- 108010014608 Proto-Oncogene Proteins c-kit Proteins 0.000 description 2
- 102000016971 Proto-Oncogene Proteins c-kit Human genes 0.000 description 2
- 102100025540 Putative olfactory receptor 8G2 Human genes 0.000 description 2
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 2
- 102000012479 Serine Proteases Human genes 0.000 description 2
- 108010022999 Serine Proteases Proteins 0.000 description 2
- 241000862969 Stella Species 0.000 description 2
- 102100032938 Telomerase reverse transcriptase Human genes 0.000 description 2
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- 102100026144 Transferrin receptor protein 1 Human genes 0.000 description 2
- 241000397921 Turbellaria Species 0.000 description 2
- COQLPRJCUIATTQ-UHFFFAOYSA-N Uranyl acetate Chemical compound O.O.O=[U]=O.CC(O)=O.CC(O)=O COQLPRJCUIATTQ-UHFFFAOYSA-N 0.000 description 2
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 2
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 2
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 210000004504 adult stem cell Anatomy 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 210000002798 bone marrow cell Anatomy 0.000 description 2
- 238000010805 cDNA synthesis kit Methods 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000036952 cancer formation Effects 0.000 description 2
- 239000002340 cardiotoxin Substances 0.000 description 2
- 231100000677 cardiotoxin Toxicity 0.000 description 2
- 239000002771 cell marker Substances 0.000 description 2
- 239000002458 cell surface marker Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 229960002424 collagenase Drugs 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 230000032459 dedifferentiation Effects 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 210000003981 ectoderm Anatomy 0.000 description 2
- 230000003511 endothelial effect Effects 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 239000003822 epoxy resin Substances 0.000 description 2
- 230000006355 external stress Effects 0.000 description 2
- 210000001508 eye Anatomy 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 102000046101 human AFP Human genes 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 238000003364 immunohistochemistry Methods 0.000 description 2
- 239000007928 intraperitoneal injection Substances 0.000 description 2
- 208000028867 ischemia Diseases 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 2
- 239000011325 microbead Substances 0.000 description 2
- 210000002894 multi-fate stem cell Anatomy 0.000 description 2
- 210000005055 nestin Anatomy 0.000 description 2
- 210000003061 neural cell Anatomy 0.000 description 2
- 210000004498 neuroglial cell Anatomy 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 210000003668 pericyte Anatomy 0.000 description 2
- 238000002135 phase contrast microscopy Methods 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 229920000647 polyepoxide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000007910 systemic administration Methods 0.000 description 2
- 230000002381 testicular Effects 0.000 description 2
- 229940124597 therapeutic agent Drugs 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 231100000588 tumorigenic Toxicity 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- JWZZKOKVBUJMES-UHFFFAOYSA-N (+-)-Isoprenaline Chemical compound CC(C)NCC(O)C1=CC=C(O)C(O)=C1 JWZZKOKVBUJMES-UHFFFAOYSA-N 0.000 description 1
- MLYCFWZIAJAIGW-UHFFFAOYSA-N 1-(2,5-dimethoxy-4-methylphenyl)butan-2-amine Chemical compound CCC(N)CC1=CC(OC)=C(C)C=C1OC MLYCFWZIAJAIGW-UHFFFAOYSA-N 0.000 description 1
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 102100030310 5,6-dihydroxyindole-2-carboxylic acid oxidase Human genes 0.000 description 1
- 102100036618 ATP-binding cassette sub-family A member 13 Human genes 0.000 description 1
- 108091005508 Acid proteases Proteins 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000009840 Angiopoietins Human genes 0.000 description 1
- 108010009906 Angiopoietins Proteins 0.000 description 1
- 102100029470 Apolipoprotein E Human genes 0.000 description 1
- 101710095339 Apolipoprotein E Proteins 0.000 description 1
- 241001064577 Ariadne <plant> Species 0.000 description 1
- 102000004580 Aspartic Acid Proteases Human genes 0.000 description 1
- 108010017640 Aspartic Acid Proteases Proteins 0.000 description 1
- 102100022716 Atypical chemokine receptor 3 Human genes 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 239000012583 B-27 Supplement Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102000004219 Brain-derived neurotrophic factor Human genes 0.000 description 1
- 108090000715 Brain-derived neurotrophic factor Proteins 0.000 description 1
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 description 1
- 238000011740 C57BL/6 mouse Methods 0.000 description 1
- 101100400452 Caenorhabditis elegans map-2 gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108090000312 Calcium Channels Proteins 0.000 description 1
- 102000003922 Calcium Channels Human genes 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 108010062745 Chloride Channels Proteins 0.000 description 1
- 102000011045 Chloride Channels Human genes 0.000 description 1
- 206010008805 Chromosomal abnormalities Diseases 0.000 description 1
- 208000031404 Chromosome Aberrations Diseases 0.000 description 1
- 108090001069 Chymopapain Proteins 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108010005843 Cysteine Proteases Proteins 0.000 description 1
- 102000005927 Cysteine Proteases Human genes 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 230000008836 DNA modification Effects 0.000 description 1
- 101100239628 Danio rerio myca gene Proteins 0.000 description 1
- 102100036411 Dermatopontin Human genes 0.000 description 1
- 101710088341 Dermatopontin Proteins 0.000 description 1
- 102100036912 Desmin Human genes 0.000 description 1
- 108010044052 Desmin Proteins 0.000 description 1
- 102100037127 Developmental pluripotency-associated protein 3 Human genes 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102400001329 Epiregulin Human genes 0.000 description 1
- 101800000155 Epiregulin Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000283074 Equus asinus Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 102000034286 G proteins Human genes 0.000 description 1
- 102100022086 GRB2-related adapter protein 2 Human genes 0.000 description 1
- 108700031843 GRB7 Adaptor Proteins 0.000 description 1
- 101150052409 GRB7 gene Proteins 0.000 description 1
- 102000030782 GTP binding Human genes 0.000 description 1
- 108091000058 GTP-Binding Proteins 0.000 description 1
- 102100022871 GTPase ERas Human genes 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 102100033107 Growth factor receptor-bound protein 7 Human genes 0.000 description 1
- 102100034339 Guanine nucleotide-binding protein G(olf) subunit alpha Human genes 0.000 description 1
- 208000031220 Hemophilia Diseases 0.000 description 1
- 208000009292 Hemophilia A Diseases 0.000 description 1
- 229920000209 Hexadimethrine bromide Polymers 0.000 description 1
- 238000010867 Hoechst staining Methods 0.000 description 1
- 101000929660 Homo sapiens ATP-binding cassette sub-family A member 13 Proteins 0.000 description 1
- 101000678890 Homo sapiens Atypical chemokine receptor 3 Proteins 0.000 description 1
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 description 1
- 101000881866 Homo sapiens Developmental pluripotency-associated protein 3 Proteins 0.000 description 1
- 101000900690 Homo sapiens GRB2-related adapter protein 2 Proteins 0.000 description 1
- 101000620820 Homo sapiens GTPase ERas Proteins 0.000 description 1
- 101000997083 Homo sapiens Guanine nucleotide-binding protein G(olf) subunit alpha Proteins 0.000 description 1
- 101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 description 1
- 101001013150 Homo sapiens Interstitial collagenase Proteins 0.000 description 1
- 101000605127 Homo sapiens Prostaglandin G/H synthase 2 Proteins 0.000 description 1
- 101001121746 Homo sapiens Putative olfactory receptor 8G2 Proteins 0.000 description 1
- 101000777245 Homo sapiens Undifferentiated embryonic cell transcription factor 1 Proteins 0.000 description 1
- 101000976622 Homo sapiens Zinc finger protein 42 homolog Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 206010021703 Indifference Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000004371 Insulin-like growth factor binding protein 5 Human genes 0.000 description 1
- 108090000961 Insulin-like growth factor binding protein 5 Proteins 0.000 description 1
- 102100027636 Insulin-like growth factor-binding protein 1 Human genes 0.000 description 1
- 108090000957 Insulin-like growth factor-binding protein 1 Proteins 0.000 description 1
- 102100025304 Integrin beta-1 Human genes 0.000 description 1
- 102000004890 Interleukin-8 Human genes 0.000 description 1
- 108090001007 Interleukin-8 Proteins 0.000 description 1
- 102100023974 Keratin, type II cytoskeletal 7 Human genes 0.000 description 1
- 108010070507 Keratin-7 Proteins 0.000 description 1
- 102100032114 Lumican Human genes 0.000 description 1
- 108010076371 Lumican Proteins 0.000 description 1
- 101150039798 MYC gene Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 208000001940 Massive Hepatic Necrosis Diseases 0.000 description 1
- 108010016113 Matrix Metalloproteinase 1 Proteins 0.000 description 1
- 102000005741 Metalloproteases Human genes 0.000 description 1
- 108010006035 Metalloproteases Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 101000900711 Mus musculus GRB2-related adaptor protein 2 Proteins 0.000 description 1
- 101100517653 Mus musculus Numbl gene Proteins 0.000 description 1
- 206010028289 Muscle atrophy Diseases 0.000 description 1
- 208000029549 Muscle injury Diseases 0.000 description 1
- 241000282339 Mustela Species 0.000 description 1
- 102000004960 NAD(P)H dehydrogenase (quinone) Human genes 0.000 description 1
- 108020000284 NAD(P)H dehydrogenase (quinone) Proteins 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 102100032063 Neurogenic differentiation factor 1 Human genes 0.000 description 1
- 108050000588 Neurogenic differentiation factor 1 Proteins 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 description 1
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 238000010220 Pearson correlation analysis Methods 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 102100038280 Prostaglandin G/H synthase 2 Human genes 0.000 description 1
- 101710121439 Proteinase-activated receptor 4 Proteins 0.000 description 1
- 101710114771 Putative olfactory receptor 8G2 Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 208000005074 Retroviridae Infections Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 238000011579 SCID mouse model Methods 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 108090001109 Thermolysin Proteins 0.000 description 1
- 102000035100 Threonine proteases Human genes 0.000 description 1
- 108091005501 Threonine proteases Proteins 0.000 description 1
- ISWQCIVKKSOKNN-UHFFFAOYSA-L Tiron Chemical compound [Na+].[Na+].OC1=CC(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C1O ISWQCIVKKSOKNN-UHFFFAOYSA-L 0.000 description 1
- 206010052779 Transplant rejections Diseases 0.000 description 1
- 102100031278 Undifferentiated embryonic cell transcription factor 1 Human genes 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000269368 Xenopus laevis Species 0.000 description 1
- 101100459258 Xenopus laevis myc-a gene Proteins 0.000 description 1
- 102100023550 Zinc finger protein 42 homolog Human genes 0.000 description 1
- 108010076089 accutase Proteins 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 239000004037 angiogenesis inhibitor Substances 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000002529 anti-mitochondrial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 206010003883 azoospermia Diseases 0.000 description 1
- 230000008436 biogenesis Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000001369 bisulfite sequencing Methods 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000002449 bone cell Anatomy 0.000 description 1
- 230000005983 bone marrow dysfunction Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000006369 cell cycle progression Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 210000001612 chondrocyte Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 229960002976 chymopapain Drugs 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 210000000795 conjunctiva Anatomy 0.000 description 1
- 208000018631 connective tissue disease Diseases 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 210000005045 desmin Anatomy 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 208000016097 disease of metabolism Diseases 0.000 description 1
- 108010007093 dispase Proteins 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000001900 endoderm Anatomy 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 210000003038 endothelium Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 210000003238 esophagus Anatomy 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 210000002768 hair cell Anatomy 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 210000005003 heart tissue Anatomy 0.000 description 1
- 210000000777 hematopoietic system Anatomy 0.000 description 1
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 description 1
- 229940096397 interleukin-8 Drugs 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 101150111214 lin-28 gene Proteins 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000002590 marrow fibroblast Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 210000003716 mesoderm Anatomy 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 239000012022 methylating agents Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 201000006938 muscular dystrophy Diseases 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 108700024542 myc Genes Proteins 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 210000000933 neural crest Anatomy 0.000 description 1
- 210000001178 neural stem cell Anatomy 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 231100001221 nontumorigenic Toxicity 0.000 description 1
- 210000004248 oligodendroglia Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229910000489 osmium tetroxide Inorganic materials 0.000 description 1
- 210000000963 osteoblast Anatomy 0.000 description 1
- 201000008968 osteosarcoma Diseases 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
- 210000001428 peripheral nervous system Anatomy 0.000 description 1
- 210000004694 pigment cell Anatomy 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 235000003784 poor nutrition Nutrition 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000022983 regulation of cell cycle Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 208000037974 severe injury Diseases 0.000 description 1
- 230000009528 severe injury Effects 0.000 description 1
- 210000002363 skeletal muscle cell Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- YEENEYXBHNNNGV-XEHWZWQGSA-M sodium;3-acetamido-5-[acetyl(methyl)amino]-2,4,6-triiodobenzoate;(2r,3r,4s,5s,6r)-2-[(2r,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound [Na+].CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I.O[C@H]1[C@H](O)[C@@H](CO)O[C@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 YEENEYXBHNNNGV-XEHWZWQGSA-M 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- WQDSRJBTLILEEK-UHFFFAOYSA-N sulfurous acid Chemical compound OS(O)=O.OS(O)=O WQDSRJBTLILEEK-UHFFFAOYSA-N 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- 108010014402 tyrosinase-related protein-1 Proteins 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 210000003932 urinary bladder Anatomy 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/10—Drugs for disorders of the urinary system of the bladder
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
- C12N2506/1353—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Transplantation (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Physical Education & Sports Medicine (AREA)
- Gynecology & Obstetrics (AREA)
- Diabetes (AREA)
- Neurology (AREA)
- Dermatology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Gastroenterology & Hepatology (AREA)
- Pregnancy & Childbirth (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21378809P | 2009-07-15 | 2009-07-15 | |
| US29015909P | 2009-12-24 | 2009-12-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2831030T3 true ES2831030T3 (es) | 2021-06-07 |
Family
ID=43449509
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES17163323T Active ES2831030T3 (es) | 2009-07-15 | 2010-07-15 | Célula madre pluripotente que puede aislarse de tejido corporal |
| ES20160074T Active ES3015175T3 (en) | 2009-07-15 | 2010-07-15 | Pluripotent stem cell that can be isolated from body tissue |
| ES10799956T Active ES2647360T5 (es) | 2009-07-15 | 2010-07-15 | Célula madre pluripotente que puede aislarse de tejido corporal |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES20160074T Active ES3015175T3 (en) | 2009-07-15 | 2010-07-15 | Pluripotent stem cell that can be isolated from body tissue |
| ES10799956T Active ES2647360T5 (es) | 2009-07-15 | 2010-07-15 | Célula madre pluripotente que puede aislarse de tejido corporal |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US9550975B2 (enExample) |
| EP (3) | EP2455452B2 (enExample) |
| JP (7) | JP5185443B2 (enExample) |
| KR (4) | KR101651795B1 (enExample) |
| CN (2) | CN107012117B (enExample) |
| AU (1) | AU2010271722B8 (enExample) |
| CA (1) | CA2768238C (enExample) |
| DK (2) | DK3214170T3 (enExample) |
| ES (3) | ES2831030T3 (enExample) |
| HR (1) | HRP20250245T1 (enExample) |
| HU (3) | HUE071170T2 (enExample) |
| NO (1) | NO2455452T3 (enExample) |
| PL (3) | PL3214170T3 (enExample) |
| PT (2) | PT2455452T (enExample) |
| SG (1) | SG177705A1 (enExample) |
| WO (1) | WO2011007900A1 (enExample) |
Families Citing this family (91)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014133703A (ja) * | 2011-03-30 | 2014-07-24 | Clio Inc | 生体組織から単離できるｓｓｅａ−３陽性の多能性幹細胞を含む他家移植用細胞治療用組成物 |
| US9399758B2 (en) | 2009-07-15 | 2016-07-26 | Mari Dezawa | SSEA3(+) pluripotent stem cell that can be isolated from body tissue |
| US9550975B2 (en) | 2009-07-15 | 2017-01-24 | Mari Dezawa | SSEA-3 pluripotent stem cell isolated from body tissue |
| JP2014132830A (ja) * | 2011-03-30 | 2014-07-24 | Clio Inc | 生体の臍帯又は脂肪組織から単離できる多能性幹細胞 |
| WO2011022507A1 (en) | 2009-08-21 | 2011-02-24 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced efficiency of induced pluripotent stem cell generation from human somatic cells |
| US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| WO2012168295A1 (en) * | 2011-06-06 | 2012-12-13 | ReGenesys BVBA | Expansion of stem cells in hollow fiber bioreactors |
| WO2013015457A1 (en) | 2011-07-27 | 2013-01-31 | Kyoto University | Novel markers for dopaminergic neuron progenitor cells |
| KR101751355B1 (ko) * | 2011-08-24 | 2017-07-04 | (주)아모레퍼시픽 | 인간 피부 진피 유래 성체 줄기세포 |
| US20140248698A1 (en) | 2011-10-21 | 2014-09-04 | Arkray, Inc. | Method for culturing pluripotency-maintained singly dispersed cells by means of laminar flow |
| WO2013131192A1 (en) * | 2012-03-06 | 2013-09-12 | Aris Aziz | Placental stem cells, methods for isolating same and use thereof |
| AU2013251649B2 (en) * | 2012-04-24 | 2019-04-04 | Vcell Therapeutics, Inc. | Generating pluripotent cells de novo |
| CA2880200A1 (en) * | 2012-07-27 | 2014-01-30 | Bioquark, Inc. | Extracts isolated from electroporated ambhibian oocytes and use thereof in treating diseases and disorders |
| WO2014027474A1 (ja) | 2012-08-17 | 2014-02-20 | 株式会社Ｃｌｉｏ | 心筋梗塞の修復再生を誘導する多能性幹細胞 |
| US20150329827A1 (en) * | 2012-12-21 | 2015-11-19 | Rutgers , The State University Of New Jersey | Muse cells isolation and expansion |
| US9709554B2 (en) | 2013-01-31 | 2017-07-18 | Rutgers, The State University Of New Jersey | In vitro model of macrosteatotic (fatty) liver |
| EP2955223B1 (en) | 2013-02-08 | 2019-12-18 | Kyoto University | Production methods for megakaryocytes and platelets |
| JPWO2014133090A1 (ja) * | 2013-02-28 | 2017-02-02 | 国立大学法人浜松医科大学 | 脳腫瘍治療のための多能性幹細胞 |
| SG10201710901RA (en) * | 2013-03-01 | 2018-02-27 | Clio Inc | Pharmaceutical composition including migratory factor for guiding pluripotent stem cells to damage |
| TW201522637A (zh) * | 2013-03-15 | 2015-06-16 | Jackson Lab | 非胚胎幹細胞之單離及其用途 |
| SG11201507829SA (en) | 2013-03-21 | 2015-12-30 | Univ Kyoto | Pluripotent stem cell for neuronal differentiation induction |
| JP6473686B2 (ja) | 2013-03-25 | 2019-02-20 | 公益財団法人神戸医療産業都市推進機構 | 細胞の選別方法 |
| JP6461787B2 (ja) | 2013-04-12 | 2019-01-30 | 国立大学法人京都大学 | 肺胞上皮前駆細胞の誘導方法 |
| WO2014185358A1 (ja) | 2013-05-14 | 2014-11-20 | 国立大学法人京都大学 | 効率的な心筋細胞の誘導方法 |
| JP2016519939A (ja) | 2013-05-22 | 2016-07-11 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ | 多能性ヒト脂肪成体幹細胞：単離、キャラクタリゼーションおよび臨床的意味 |
| WO2015017500A1 (en) | 2013-07-30 | 2015-02-05 | Musculoskeletal Transplant Foundation | Acellular soft tissue-derived matrices and methods for preparing same |
| JP6378183B2 (ja) | 2013-08-07 | 2018-08-22 | 国立大学法人京都大学 | 膵ホルモン産生細胞の製造法 |
| US10265347B2 (en) | 2013-08-29 | 2019-04-23 | Norimasa Miura | Biomolecular group related to cell anti-aging |
| EP3048169B1 (en) | 2013-09-04 | 2019-07-10 | Otsuka Pharmaceutical Factory, Inc. | Method for preparing pluripotent stem cells |
| CN121136933A (zh) | 2013-09-05 | 2025-12-16 | 国立大学法人京都大学 | 由多能干细胞制造产多巴胺神经前体细胞的方法、产多巴胺神经前体细胞、帕金森病治疗剂和试剂盒 |
| EP3064577B1 (en) | 2013-11-01 | 2020-09-09 | Kyoto University | Novel chondrocyte induction method |
| WO2015081094A1 (en) * | 2013-11-27 | 2015-06-04 | University Of Louisville Research Foundation, Inc. | Cardiac progenitor cells and methods of use therefor |
| JP6519038B2 (ja) * | 2014-02-26 | 2019-05-29 | 株式会社生命科学インスティテュート | 脳梗塞治療のための多能性幹細胞 |
| JP2015160820A (ja) * | 2014-02-26 | 2015-09-07 | 株式会社Ｃｌｉｏ | 慢性腎障害治療のための多能性幹細胞 |
| JP2017513517A (ja) | 2014-03-19 | 2017-06-01 | ブイセル セラピューティックス，インコーポレイテッド | 多能性細胞に関連する方法 |
| CN106536718B (zh) | 2014-05-21 | 2021-04-27 | 国立大学法人京都大学 | 胰芽细胞的制造方法及含有胰芽细胞的胰疾病治疗剂 |
| KR101592401B1 (ko) * | 2014-06-13 | 2016-02-05 | 주식회사 비비에이치씨 | 지방-유래 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 및 그 방법에 의해 제조된 유도만능 줄기세포 |
| JP6391364B2 (ja) * | 2014-08-25 | 2018-09-19 | 国立大学法人 鹿児島大学 | 認知機能障害改善用細胞製剤 |
| JP6452107B2 (ja) | 2014-09-05 | 2019-01-16 | 国立大学法人 東京大学 | 糖尿病性皮膚潰瘍治療のための多能性幹細胞 |
| KR101700598B1 (ko) * | 2014-11-11 | 2017-01-31 | 한국원자력의학원 | Cd105를 포함하는 줄기세포능을 가진 섬유아세포 분리용 바이오마커 조성물 및 이를 이용한 줄기세포능을 가진 섬유아세포 분리 방법 |
| CA2986702C (en) | 2015-05-21 | 2023-04-04 | David Wang | Modified demineralized cortical bone fibers |
| WO2016194816A1 (ja) * | 2015-05-29 | 2016-12-08 | 日本臓器製薬株式会社 | 多能性幹細胞遊走促進剤 |
| US10912864B2 (en) | 2015-07-24 | 2021-02-09 | Musculoskeletal Transplant Foundation | Acellular soft tissue-derived matrices and methods for preparing same |
| US11052175B2 (en) | 2015-08-19 | 2021-07-06 | Musculoskeletal Transplant Foundation | Cartilage-derived implants and methods of making and using same |
| JP6627027B2 (ja) * | 2015-09-11 | 2020-01-08 | 福美人株式会社 | 皮膚細胞の活性化作用を呈するフラボノイド誘導体 |
| JP6940101B2 (ja) * | 2016-01-15 | 2021-09-22 | 国立大学法人富山大学 | 虚血性脳梗塞への多能性幹細胞の動員 |
| JP6770673B2 (ja) * | 2016-02-26 | 2020-10-21 | 国立大学法人東海国立大学機構 | 急性心筋梗塞の予後マーカー及びその利用 |
| AU2017235653B2 (en) | 2016-03-17 | 2021-12-16 | Synova Life Sciences, Inc. | Separation, dissociation and/or disaggregation of cells using shockwaves or mechanical impacts |
| EP3447130A4 (en) | 2016-04-22 | 2019-11-13 | Kyoto University | METHOD FOR PRODUCING DOPAMINE-PROPERING NEURAL PRESERVATOR CELLS |
| SG11201810188VA (en) * | 2016-05-16 | 2019-02-27 | Univ Nagoya Nat Univ Corp | Amelioration and treatment of perinatal brain damage with pluripotent stem cells |
| US20190201455A1 (en) | 2016-07-01 | 2019-07-04 | Tohoku University | Prophylactic or therapeutic agent for organ fibrosis |
| WO2018021515A1 (ja) | 2016-07-29 | 2018-02-01 | 国立大学法人東北大学 | 血管障害の予防又は治療剤 |
| SG11201900983TA (en) | 2016-08-03 | 2019-03-28 | Univ Nagoya Nat Univ Corp | Amelioration and treatment of chronic lung disease using pluripotent stem cells |
| US11920180B2 (en) | 2016-08-03 | 2024-03-05 | Tohoku University | Method for inducing differentiation of pluripotent stem cells in vitro |
| CA3032925A1 (en) | 2016-08-03 | 2018-02-08 | Life Science Institute, Inc. | Alleviation and treatment of ischemia reperfusion-induced lung injury using pluripotent stem cells |
| EP3578641A4 (en) | 2017-02-03 | 2020-11-11 | Kyushu University National University Corporation | PROCESS FOR PREPARING DENTAL PULP STEM CELLS FROM CELLS DERIVED FROM DENTAL PULP TISSUE |
| EP3604503B1 (en) * | 2017-03-28 | 2026-03-18 | Ajinomoto Co., Inc. | Additive for undifferentiation maintaining medium |
| JP7712643B2 (ja) * | 2017-06-19 | 2025-07-24 | 国立大学法人東北大学 | 表皮水疱症の治療剤 |
| CN110869034A (zh) | 2017-06-20 | 2020-03-06 | 国立大学法人名古屋大学 | 利用多能干细胞进行的伴随胎儿生长迟缓的脑损伤的改善和治疗 |
| WO2019078262A1 (ja) | 2017-10-17 | 2019-04-25 | 国立大学法人広島大学 | 骨軟骨修復を誘導する多能性幹細胞 |
| AU2018364852B2 (en) * | 2017-11-10 | 2022-06-30 | Regenesis Science Co., Ltd. | Method for producing cultured cell, and method for producing therapeutic agent for spinal cord injury disease |
| WO2019107913A1 (ko) | 2017-11-28 | 2019-06-06 | 주식회사 셀투인 | 실시간 글루타치온 측정을 통한 치료용 세포의 품질 향상 방법 |
| JP6604492B2 (ja) * | 2018-04-09 | 2019-11-13 | 株式会社生命科学インスティテュート | 脳梗塞治療のための多能性幹細胞 |
| SG11202011127WA (en) * | 2018-05-09 | 2020-12-30 | Life Science Institute Inc | Therapeutic agent for spinal cord injury |
| JP7072777B2 (ja) * | 2018-07-03 | 2022-05-23 | 株式会社生命科学インスティテュート | 慢性腎障害治療のための多能性幹細胞 |
| US20210299331A1 (en) | 2018-07-19 | 2021-09-30 | Kyoto University | Pluripotent stem cell-derived plate-shaped cartilage and method for producing the same |
| EP3882340B1 (en) * | 2018-11-14 | 2025-01-15 | Kataoka Corporation | Method for producing insulin-producing cells, and composition |
| WO2020111249A1 (ja) | 2018-11-30 | 2020-06-04 | 京都府公立大学法人 | 末梢血流障害の治療剤 |
| WO2020130147A1 (ja) | 2018-12-21 | 2020-06-25 | 国立大学法人京都大学 | ルブリシン局在軟骨様組織、その製造方法及びそれを含む関節軟骨損傷治療用組成物 |
| JP2022528965A (ja) * | 2019-04-09 | 2022-06-16 | ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー | 細胞集団を濃縮する方法 |
| CA3150769A1 (en) | 2019-08-09 | 2021-02-18 | Tohoku University | Agent for treating or preventing vascular dementia |
| CA3153237A1 (en) | 2019-09-05 | 2021-03-11 | Tohoku University | Therapeutic agent for myocarditis |
| US12577536B2 (en) | 2019-10-01 | 2026-03-17 | Kyoto University | Method for isolating ureteric bud tip cells |
| CA3156700A1 (en) * | 2019-10-31 | 2021-05-06 | Life Science Institute, Inc. | Therapy for interstitial cystitis by pluripotent stem cells |
| JP7058431B2 (ja) | 2019-12-12 | 2022-04-22 | 国立大学法人千葉大学 | 巨核球および血小板を含む凍結乾燥製剤 |
| EP4123016A4 (en) | 2020-03-19 | 2024-06-19 | Orizuru Therapeutics, Inc. | Method for purifying cardiomyocytes |
| WO2021187601A1 (ja) | 2020-03-19 | 2021-09-23 | 国立大学法人京都大学 | 心筋細胞の精製方法 |
| US20230203436A1 (en) | 2020-04-02 | 2023-06-29 | Tohoku University | High-potential pluripotent stem cells |
| US20230256024A1 (en) | 2020-07-13 | 2023-08-17 | Kyoto University | Skeletal muscle precursor cells and method for purifying same, composition for treating myogenic diseases, and method for producing cell group containing skeletal muscle precursor cells |
| CA3186061A1 (en) * | 2020-07-15 | 2022-01-20 | Koji Abe | Pluripotent stem cells effective for treatment of motor neuron disease (mnd) |
| JP2021073305A (ja) * | 2021-02-09 | 2021-05-13 | 株式会社生命科学インスティテュート | 慢性腎障害治療のための多能性幹細胞 |
| TWI832070B (zh) * | 2021-07-15 | 2024-02-11 | 國璽幹細胞應用技術股份有限公司 | 幹細胞製劑用於治療關節炎的用途及用於製備幹細胞製劑的方法 |
| JP2023041486A (ja) * | 2021-09-13 | 2023-03-24 | 国立大学法人東北大学 | 毛髪損傷の治療剤 |
| JPWO2023153464A1 (enExample) | 2022-02-09 | 2023-08-17 | ||
| EP4530344A1 (en) | 2022-05-23 | 2025-04-02 | Kyoto University | Production method for renal collecting duct cells and pelvic epithelial cells |
| CN121219404A (zh) | 2023-04-03 | 2025-12-26 | 大塚制药株式会社 | 巨核细胞的制造方法 |
| WO2024248017A1 (ja) | 2023-05-29 | 2024-12-05 | 国立研究開発法人理化学研究所 | 心筋細胞を成熟させる方法及び成熟した心筋細胞の製造方法 |
| CN117187174B (zh) * | 2023-11-08 | 2024-02-06 | 广州正源生物技术有限公司 | 一种Muse细胞培养基以及一种脂肪Muse细胞的提取方法 |
| JP2025123077A (ja) * | 2024-02-09 | 2025-08-22 | 国立大学法人東北大学 | 脊髄梗塞治療のための多能性幹細胞 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1226233B1 (en) | 1999-08-05 | 2011-06-29 | ABT Holding Company | Multipotent adult stem cells and methods for isolation |
| US7575921B2 (en) * | 1999-12-30 | 2009-08-18 | Vbi Technologies, L.L.C. | Spore-like cells and uses thereof |
| US20020151050A1 (en) * | 2000-10-30 | 2002-10-17 | Vacanti Charles A. | Isolation of spore-like cells from tissues exposed to extreme conditions |
| JP4182742B2 (ja) | 2002-12-12 | 2008-11-19 | 日産自動車株式会社 | 自動車の前部車体構造 |
| US7569385B2 (en) | 2003-08-14 | 2009-08-04 | The Regents Of The University Of California | Multipotent amniotic fetal stem cells |
| WO2005021738A1 (en) * | 2003-08-29 | 2005-03-10 | Regents Of The University Of Minnesota | Kidney derived stem cells and methods for their isolation, differentiation and use |
| US20060216821A1 (en) * | 2004-02-26 | 2006-09-28 | Reliance Life Sciences Pvt. Ltd. | Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof |
| CN101180389A (zh) * | 2005-05-17 | 2008-05-14 | 利莱恩斯生命科学有限公司 | 采用哺乳动物细胞确立人胚胎干细胞系 |
| US9388382B2 (en) * | 2005-10-05 | 2016-07-12 | The Board Of Trustees Of The University Of Illinois | Isolation of CD14 negative, CD45 positive and CD117 positive embryonic-like stem cells free of monocytes from human umbilical cord blood mononuclear cells |
| AU2006304390A1 (en) | 2005-10-17 | 2007-04-26 | Academia Sinica | Pulmonary stem cells, related methods and kits |
| EP2206724A1 (en) | 2005-12-13 | 2010-07-14 | Kyoto University | Nuclear reprogramming factor |
| WO2008080200A1 (en) | 2006-12-29 | 2008-07-10 | Irina Kerkis | Process for obtaining stem cells |
| JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| CN101978046A (zh) * | 2007-10-30 | 2011-02-16 | 路易斯维尔大学研究基金会有限公司 | 非常小的胚胎样(vsel)干细胞的应用和分离 |
| US20090172832A1 (en) * | 2007-12-28 | 2009-07-02 | Timothy Caspar | Expression of rubisco enzyme from a non-rubisco locus |
| ITRM20080342A1 (it) * | 2008-06-26 | 2009-12-27 | Univ Degli Studi Udine | Cellule di polpa dentale midollo-simili, metodi per isolamento ed uso. |
| US9550975B2 (en) * | 2009-07-15 | 2017-01-24 | Mari Dezawa | SSEA-3 pluripotent stem cell isolated from body tissue |
-
2010
- 2010-07-14 US US12/836,264 patent/US9550975B2/en active Active
- 2010-07-15 PL PL17163323T patent/PL3214170T3/pl unknown
- 2010-07-15 ES ES17163323T patent/ES2831030T3/es active Active
- 2010-07-15 HU HUE20160074A patent/HUE071170T2/hu unknown
- 2010-07-15 KR KR1020157013171A patent/KR101651795B1/ko active Active
- 2010-07-15 DK DK17163323.3T patent/DK3214170T3/da active
- 2010-07-15 CN CN201611060144.1A patent/CN107012117B/zh active Active
- 2010-07-15 KR KR1020127003879A patent/KR20120069663A/ko not_active Ceased
- 2010-07-15 WO PCT/JP2010/062480 patent/WO2011007900A1/ja not_active Ceased
- 2010-07-15 NO NO10799956A patent/NO2455452T3/no unknown
- 2010-07-15 PL PL10799956T patent/PL2455452T5/pl unknown
- 2010-07-15 ES ES20160074T patent/ES3015175T3/es active Active
- 2010-07-15 EP EP10799956.7A patent/EP2455452B2/en active Active
- 2010-07-15 KR KR1020167008209A patent/KR101764080B1/ko active Active
- 2010-07-15 JP JP2011522886A patent/JP5185443B2/ja active Active
- 2010-07-15 PL PL20160074.9T patent/PL3680323T3/pl unknown
- 2010-07-15 CA CA2768238A patent/CA2768238C/en active Active
- 2010-07-15 EP EP20160074.9A patent/EP3680323B1/en active Active
- 2010-07-15 CN CN201080041908.7A patent/CN102858951B/zh active Active
- 2010-07-15 HU HUE10799956A patent/HUE037448T2/hu unknown
- 2010-07-15 SG SG2012003836A patent/SG177705A1/en unknown
- 2010-07-15 EP EP17163323.3A patent/EP3214170B1/en active Active
- 2010-07-15 HU HUE17163323A patent/HUE052559T2/hu unknown
- 2010-07-15 ES ES10799956T patent/ES2647360T5/es active Active
- 2010-07-15 PT PT107999567T patent/PT2455452T/pt unknown
- 2010-07-15 DK DK10799956.7T patent/DK2455452T4/da active
- 2010-07-15 AU AU2010271722A patent/AU2010271722B8/en active Active
- 2010-07-15 HR HRP20250245TT patent/HRP20250245T1/hr unknown
- 2010-07-15 KR KR1020177020765A patent/KR101945343B1/ko active Active
- 2010-07-15 PT PT171633233T patent/PT3214170T/pt unknown
-
2012
- 2012-05-01 JP JP2012104994A patent/JP5852503B2/ja active Active
-
2015
- 2015-12-04 JP JP2015237186A patent/JP6092357B2/ja active Active
-
2017
- 2017-02-08 JP JP2017021036A patent/JP6416298B2/ja active Active
-
2018
- 2018-10-03 JP JP2018188234A patent/JP6641441B2/ja active Active
-
2019
- 2019-12-27 JP JP2019239248A patent/JP6937821B2/ja active Active
-
2021
- 2021-08-31 JP JP2021140656A patent/JP7134317B2/ja active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2647360T5 (es) | Célula madre pluripotente que puede aislarse de tejido corporal | |
| US11261426B2 (en) | Pluripotent stem cell that can be isolated from body tissue | |
| WO2012133942A1 (ja) | 生体の臍帯又は脂肪組織から単離できる多能性幹細胞 |