ES2571446T3 - ADN polimerasas quiméricas - Google Patents

ADN polimerasas quiméricas

Info

Publication number
ES2571446T3
ES2571446T3 ES09829689T ES09829689T ES2571446T3 ES 2571446 T3 ES2571446 T3 ES 2571446T3 ES 09829689 T ES09829689 T ES 09829689T ES 09829689 T ES09829689 T ES 09829689T ES 2571446 T3 ES2571446 T3 ES 2571446T3
Authority
ES
Spain
Prior art keywords
chimeric dna
domain
dna polymerases
polymerase
palm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09829689T
Other languages
English (en)
Spanish (es)
Inventor
Bjarne Faurholm
Paul Mcewan
William Bourn
Gavin Rush
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kapa Biosystems Inc
Original Assignee
Kapa Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kapa Biosystems Inc filed Critical Kapa Biosystems Inc
Application granted granted Critical
Publication of ES2571446T3 publication Critical patent/ES2571446T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES09829689T 2008-11-03 2009-11-03 ADN polimerasas quiméricas Active ES2571446T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11086208P 2008-11-03 2008-11-03
PCT/US2009/063166 WO2010062776A2 (en) 2008-11-03 2009-11-03 Chimeric dna polymerases

Publications (1)

Publication Number Publication Date
ES2571446T3 true ES2571446T3 (es) 2016-05-25

Family

ID=42226349

Family Applications (2)

Application Number Title Priority Date Filing Date
ES09829689T Active ES2571446T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas
ES15160891T Active ES2716402T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES15160891T Active ES2716402T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas

Country Status (7)

Country Link
US (2) US9023633B2 (cg-RX-API-DMAC7.html)
EP (2) EP2927317B1 (cg-RX-API-DMAC7.html)
JP (3) JP2012507986A (cg-RX-API-DMAC7.html)
CN (1) CN102257136A (cg-RX-API-DMAC7.html)
CA (1) CA2742593C (cg-RX-API-DMAC7.html)
ES (2) ES2571446T3 (cg-RX-API-DMAC7.html)
WO (1) WO2010062776A2 (cg-RX-API-DMAC7.html)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102245761A (zh) 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
CN102392000A (zh) * 2011-11-07 2012-03-28 李志茹 一种耐高温Pyrolobus聚合酶及其高效表达质粒和应用
CN103898077B (zh) 2012-12-24 2017-01-11 财团法人工业技术研究院 单离的脱氧核糖核酸聚合酶、套组及其应用
CN104250641B (zh) * 2013-06-28 2017-09-01 北京福安华生物科技有限公司 一种高保真dna聚合酶及其制备和应用
WO2015019658A1 (ja) * 2013-08-06 2015-02-12 東洋紡株式会社 核酸増幅法
AU2015279794B2 (en) * 2014-06-27 2021-09-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
WO2016033315A2 (en) * 2014-08-27 2016-03-03 New England Biolabs, Inc. Synthon formation
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
CN114517189A (zh) * 2014-11-14 2022-05-20 亿明达股份有限公司 聚合酶
JP6619152B2 (ja) * 2015-05-08 2019-12-11 国立研究開発法人産業技術総合研究所 フラビンアデニンジヌクレオチド依存型グルコース脱水素酵素活性を有するキメラタンパク質
CN106867978A (zh) * 2015-12-10 2017-06-20 江苏众红生物工程创药研究院有限公司 一种重组高保真dna聚合酶及其编码基因和表达方法
JP6969089B2 (ja) * 2015-12-11 2021-11-24 東洋紡株式会社 核酸増幅用組成物及び核酸増幅法
JP6980375B2 (ja) * 2015-12-11 2021-12-15 東洋紡株式会社 核酸増幅用試薬及び核酸増幅法
US10626383B2 (en) 2016-01-15 2020-04-21 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
EP3405578B1 (en) * 2016-01-19 2022-03-16 Board of Regents, The University of Texas System Thermostable reverse transcriptase
EP3458597B1 (en) 2016-05-18 2022-09-07 Roche Diagnostics GmbH Quantitative real time pcr amplification using an electrowetting-based device
FR3052462A1 (fr) * 2016-06-14 2017-12-15 Dna Script Variants d'une adn polymerase de la famille polx
CN106011100A (zh) * 2016-08-08 2016-10-12 吴江近岸蛋白质科技有限公司 一种Pfu DNA聚合酶及其制备方法
KR20230052995A (ko) * 2016-12-19 2023-04-20 퀀텀-에스아이 인코포레이티드 시퀀싱 반응을 위한 중합 효소
CN106754816B (zh) * 2017-02-24 2020-10-27 依科赛生物科技（太仓）有限公司 一种高保真快速扩增融合酶及其制备方法
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
EP3645711A4 (en) 2017-06-30 2021-04-21 Codexis, Inc. T7 RNA POLYMERASE VARIANTS
JP2020530267A (ja) 2017-06-30 2020-10-22 コデクシス， インコーポレイテッド Ｔ７ ｒｎａポリメラーゼバリアント
EP4530346A3 (en) * 2017-07-12 2025-06-11 Genecast Co., Ltd. Dna polymerase with increased gene mutation specificity and pcr buffer composition for increasing activity thereof
WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
EP3740591A4 (en) 2018-01-19 2022-02-23 Bio-Rad Laboratories, Inc. Mutant dna polymerases
CN112639089B (zh) * 2018-09-03 2024-02-09 深圳华大生命科学研究院 重组型kod聚合酶
CN112673098B (zh) * 2018-10-31 2023-01-06 亿明达股份有限公司 聚合酶、组合物及使用方法
IL297994A (en) 2018-12-05 2025-01-01 Illumina Inc Polymerases, compounds, and methods of use
CN109628424B (zh) * 2018-12-31 2022-04-26 苏州近岸蛋白质科技股份有限公司 一种新型嵌合dna聚合酶及其制备方法
GB2587177A (en) * 2019-01-09 2021-03-24 3Cr Bioscience Ltd Polymerase
WO2020144480A1 (en) * 2019-01-09 2020-07-16 3Cr Bioscience Ltd. Method for detecting a nucleic acid sequence
US11959105B2 (en) 2019-06-28 2024-04-16 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
US20240200040A1 (en) * 2019-12-23 2024-06-20 Bgi Shenzhen Chimeric dna polymerase and application thereof
EP4103747A4 (en) * 2020-02-15 2024-03-13 Agency for Science, Technology and Research A method to amplify a nucleic acid
CN113604450B (zh) * 2021-08-18 2023-08-18 华南理工大学 一种kod dna聚合酶突变体及其制备方法与应用
CN113755465A (zh) * 2021-09-23 2021-12-07 武汉爱博泰克生物科技有限公司 嵌合体dna聚合酶及其制备方法
EP4435098A4 (en) * 2021-11-15 2025-10-01 Bgi Shenzhen CHIMERIC DNA POLYMERASE AND ITS USE
CN119736271A (zh) * 2024-12-27 2025-04-01 安序源生物科技(深圳)有限公司 一种phi29 DNA聚合酶突变体及其用途

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5948663A (en) 1990-12-03 1999-09-07 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5866395A (en) 1990-12-03 1999-02-02 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
DE547359T1 (de) * 1991-12-18 1993-11-25 New England Biolabs Inc Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten.
US5556772A (en) 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
JP3132624B2 (ja) * 1994-05-09 2001-02-05 東洋紡績株式会社 超好熱始原菌由来のｄｎａポリメラーゼ遺伝子およびその用途
US6033859A (en) * 1996-05-24 2000-03-07 Toyo Boseki Kabushiki Kaisha Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1
WO1998033900A1 (en) * 1997-01-31 1998-08-06 Japan Science And Technology Corporation Method and apparatus for predicting protein function site, method for improving protein function, and function-improved protein
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
IL141481A0 (en) 1998-08-18 2002-03-10 Dermtech Int Method for detection of biological factors in epidermis
GB9921318D0 (en) 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
AU1239901A (en) 1999-10-29 2001-05-14 Stratagene Compositions and methods utilizing dna polymerases
ATE395420T1 (de) 2000-02-17 2008-05-15 Qiagen Gmbh Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
DE60141097D1 (de) 2000-05-11 2010-03-11 Toyo Boseki Modifizierte thermostabile dna polymerase von pyrococcus kodakaraensis
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
WO2002004022A1 (en) 2000-07-12 2002-01-17 Invitrogen Corp High fidelity polymerases and uses thereof
GB2382321A (en) 2001-11-21 2003-05-28 Doyen Medipharm Ltd Process and apparatus for cutting sheets
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
US7704712B2 (en) 2003-03-25 2010-04-27 Stratagene California DNA polymerase fusions and uses thereof
US20040244310A1 (en) 2003-03-28 2004-12-09 Blumberg Marvin R. Data center
AU2005245966B2 (en) 2004-05-20 2010-04-01 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Thermostable Y-family polymerases and chimeras
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
US20070190538A1 (en) 2005-07-29 2007-08-16 Applera Corporation Chimeric polymerases
JP2009521227A (ja) 2005-12-22 2009-06-04 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド ヌクレオチドアナログ組込み用ポリメラーゼ
JP2007298879A (ja) 2006-05-02 2007-11-15 C & C:Kk ストロボ装置
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
EP2089517A4 (en) 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
WO2010062779A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Modified dna polymerases
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
JP5328696B2 (ja) 2010-03-01 2013-10-30 淀川ヒューテック株式会社 複層ベルト

Also Published As

Publication number Publication date
US9023633B2 (en) 2015-05-05
EP2352818A4 (en) 2011-10-12
ES2716402T3 (es) 2019-06-12
CN102257136A (zh) 2011-11-23
EP2352818A2 (en) 2011-08-10
WO2010062776A3 (en) 2010-08-19
JP6150847B2 (ja) 2017-06-21
CA2742593A1 (en) 2010-06-03
WO2010062776A2 (en) 2010-06-03
CA2742593C (en) 2020-05-05
EP2927317B1 (en) 2018-12-19
EP2927317A1 (en) 2015-10-07
EP2352818B1 (en) 2016-02-10
US20120115188A1 (en) 2012-05-10
JP2015154787A (ja) 2015-08-27
JP2017029172A (ja) 2017-02-09
JP2012507986A (ja) 2012-04-05
US20140363848A1 (en) 2014-12-11
US9388396B2 (en) 2016-07-12
HK1216032A1 (en) 2016-10-07

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