ES2568803T3 - Reducción del contenido de ácidos grasos saturados en semillas de plantas - Google Patents

Reducción del contenido de ácidos grasos saturados en semillas de plantas Download PDF

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Publication number
ES2568803T3
ES2568803T3 ES11799019.2T ES11799019T ES2568803T3 ES 2568803 T3 ES2568803 T3 ES 2568803T3 ES 11799019 T ES11799019 T ES 11799019T ES 2568803 T3 ES2568803 T3 ES 2568803T3
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Prior art keywords
reduction
content
fatty acids
seq
saturated fatty
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ES11799019.2T
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Inventor
Ann Owens Merlo
Daniel J GACHOTTE
Mark A. Thompson
Terence A. Walsh
Scott Bevan
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Corteva Agriscience LLC
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Dow AgroSciences LLC
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083Miscellaneous (1.14.99)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/34Vector systems having a special element relevant for transcription being a transcription initiation element
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/36Vector systems having a special element relevant for transcription being a transcription termination element

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Una molécula de ácido nucleico aislada que codifica una enzima de delta-9 desaturasa que comprende una secuencia de aminoácidos que es al menos 80% idéntica a SEQ ID NO:12, en donde la molécula de ácido nucleico comprende una secuencia de nucleótidos al menos 60% idéntica a una secuencia seleccionada del grupo que consiste en SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:9, and SEQ ID NO:15.

Description

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ES11799019.2T 2010-06-24 2011-06-24 Reducción del contenido de ácidos grasos saturados en semillas de plantas Active ES2568803T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35831410P 2010-06-24 2010-06-24
US358314P 2010-06-24
PCT/US2011/041897 WO2011163632A2 (en) 2010-06-24 2011-06-24 Lowering saturated fatty acid content of plant seeds

Publications (1)

Publication Number Publication Date
ES2568803T3 true ES2568803T3 (es) 2016-05-04

Family

ID=45372139

Family Applications (3)

Application Number Title Priority Date Filing Date
ES14191879.7T Active ES2620492T3 (es) 2010-06-24 2011-06-24 Reducción del contenido de ácidos grasos saturados en semillas de plantas
ES11799019.2T Active ES2568803T3 (es) 2010-06-24 2011-06-24 Reducción del contenido de ácidos grasos saturados en semillas de plantas
ES14191880T Active ES2623454T3 (es) 2010-06-24 2011-06-24 Reducción del contenido de ácidos grasos saturados en semillas de plantas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES14191879.7T Active ES2620492T3 (es) 2010-06-24 2011-06-24 Reducción del contenido de ácidos grasos saturados en semillas de plantas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES14191880T Active ES2623454T3 (es) 2010-06-24 2011-06-24 Reducción del contenido de ácidos grasos saturados en semillas de plantas

Country Status (20)

Country Link
US (1) US20110302672A1 (es)
EP (3) EP2862930B1 (es)
JP (5) JP6096114B2 (es)
KR (3) KR20180008885A (es)
CN (3) CN103068986B (es)
AR (1) AR081303A1 (es)
AU (1) AU2011270652B2 (es)
BR (1) BR112012033112A2 (es)
CA (3) CA2999347C (es)
CL (5) CL2012003665A1 (es)
DK (3) DK2585600T3 (es)
EA (1) EA029904B1 (es)
ES (3) ES2620492T3 (es)
IL (3) IL223438A (es)
MX (3) MX350491B (es)
NZ (3) NZ603971A (es)
TW (4) TW201634693A (es)
UA (1) UA115302C2 (es)
WO (1) WO2011163632A2 (es)
ZA (1) ZA201209083B (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9617555B2 (en) 2004-10-08 2017-04-11 Dow Agrosciences Llc Generation of transgenic canola with low or no saturated fatty acids
BR102012019436B8 (pt) * 2011-07-26 2022-10-11 Dow Agrosciences Llc Método de detecção do evento de soja pdab9582.814.19.1
BR102012019434B1 (pt) 2011-07-26 2021-11-09 Dow Agrosciences Llc Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1
JP2017537633A (ja) * 2014-12-19 2017-12-21 ダウ アグロサイエンシィズ エルエルシー 低飽和脂肪酸または無飽和脂肪酸トランスジェニックキャノーラの生成
CN106755017A (zh) * 2017-01-09 2017-05-31 程佳祎 一种纳豆激酶的转基因方法
CN109880849B (zh) * 2019-01-03 2023-04-11 四川大学 靶向肿瘤相关巨噬细胞的GHOST-shRNA表达载体复合物及其应用
CN111560394A (zh) * 2019-12-30 2020-08-21 海南大学 一种利用种子荧光快速鉴定转基因拟南芥的方法
KR102566555B1 (ko) * 2020-05-20 2023-08-14 한국화학연구원 델타-9-지방산불포화 효소를 암호화 하는 유전자 및 이의 용도
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
CA3191387A1 (en) 2020-09-30 2022-04-07 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
KR20230046412A (ko) 2021-09-30 2023-04-06 세종대학교산학협력단 종자 내 포화지방산 함량 증가용 조성물 및 방법
CN115807014A (zh) * 2022-07-01 2023-03-17 西南大学 桑蚕脂肪酸脱氢酶Bmdesat5的应用

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5504200A (en) 1983-04-15 1996-04-02 Mycogen Plant Science, Inc. Plant gene expression
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
NZ221259A (en) 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
ES2039474T3 (es) 1986-12-05 1993-10-01 Ciba-Geigy Ag Procedimineto mejorado para la transformacion de protoplastos vegetales.
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
JP3209744B2 (ja) 1990-01-22 2001-09-17 デカルブ・ジェネティクス・コーポレーション 結実能力のある遺伝子変換コーン
US7037692B1 (en) 1990-03-16 2006-05-02 Calgene, Inc. Plant desaturases compositions and uses
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US5518908A (en) 1991-09-23 1996-05-21 Monsanto Company Method of controlling insects
JPH0614667A (ja) * 1992-03-13 1994-01-25 Lubrizol Corp:The デサチュラーゼを使用しての植物油の改変
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
ES2330168T3 (es) 1995-10-13 2009-12-04 Dow Agrosciences Llc Gen mopdificado de bacillus thuringiensis para combatir los lepidopteros en plantas.
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
DK1068342T3 (da) * 1998-03-30 2009-11-02 Dow Agrosciences Llc Modifikation af fedtsyresammensætning i planter ved ekspression af en Aspergillus nidulans delta-9 CoA desaturase
AU5785199A (en) 1998-08-24 2000-03-14 Rutgers, The State University Of New Jersey Synthetic fatty acid desaturase gene for expression in plants
JP2002534129A (ja) 1999-01-14 2002-10-15 モンサント テクノロジー エルエルシー ダイズ形質転換方法
US6784342B1 (en) 1999-08-04 2004-08-31 The University Of British Columbia Regulation of embryonic transcription in plants
US7087812B1 (en) 1999-09-30 2006-08-08 Japan Tobacco Inc. Vectors for transforming plants
US7253337B2 (en) 2000-05-24 2007-08-07 The University Of British Columbia Gene regulatory region that promotes early seed-specific transcription
US6706950B2 (en) 2000-07-25 2004-03-16 Calgene Llc Nucleic acid sequences encoding β-ketoacyl-ACP synthase and uses thereof
US7045683B2 (en) * 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
JP2005185101A (ja) * 2002-05-30 2005-07-14 National Institute Of Agrobiological Sciences 植物の全長cDNAおよびその利用
CA2450000A1 (en) * 2003-12-18 2005-06-18 Alberta Research Council Inc. Method of creating plants with reduced level of saturated fatty acid in seed oil
PT1766023E (pt) * 2004-06-04 2010-12-09 Fluxome Sciences As Células metabolicamente manipuladas para a produção de ácidos gordos poliinsaturados
US20080260933A1 (en) * 2004-10-08 2008-10-23 Dow Agroscience Llc Certain Plants with "No Saturate" or Reduced Saturate Levels of Fatty Acids in Seeds, and Oil Derived from the Seeds
MX2007004231A (es) * 2004-10-08 2007-06-14 Dow Agrosciences Llc Ciertas plantas con concentraciones de acidos grasos "no saturados" o saturados reducidos en semillas y aceite derivado de las semillas._.
EP1827079A4 (en) * 2004-12-21 2012-04-11 Monsanto Technology Llc TRANSGENIC PLANTS HAVING SUPERIOR AGRONOMIC CHARACTERS
US20090070897A1 (en) * 2006-01-12 2009-03-12 Goldman Barry S Genes and uses for plant improvement
EP2489726A3 (en) * 2005-01-12 2012-11-28 Monsanto Technology LLC Genes and uses for plant improvement
WO2007131720A1 (en) * 2006-05-11 2007-11-22 Cosmoferm B.V. Improved production of sphingoid bases using genetically engineered microbial strains
US8329994B2 (en) * 2008-10-14 2012-12-11 Monsanto Technology Llc Utilization of fatty acid desaturases from Hemiselmis spp

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Publication number Publication date
TW201634696A (zh) 2016-10-01
CN103068986B (zh) 2017-03-29
JP6186463B2 (ja) 2017-08-23
JP6186462B2 (ja) 2017-08-23
EP2585600B1 (en) 2016-02-03
JP2016144456A (ja) 2016-08-12
JP2016136958A (ja) 2016-08-04
CL2016000903A1 (es) 2016-10-14
EP2585600A2 (en) 2013-05-01
BR112012033112A2 (pt) 2015-09-08
MX338726B (es) 2016-04-28
CA2803098C (en) 2019-05-21
EP2862931B1 (en) 2017-01-25
IL244003A0 (en) 2016-04-21
JP2016136957A (ja) 2016-08-04
DK2862930T3 (en) 2017-05-22
UA115302C2 (uk) 2017-10-25
DK2862931T3 (en) 2017-05-01
EP2862930A1 (en) 2015-04-22
DK2585600T3 (da) 2016-05-09
AU2011270652B2 (en) 2015-10-01
ES2623454T3 (es) 2017-07-11
US20110302672A1 (en) 2011-12-08
CA2999347A1 (en) 2011-12-29
CA2999347C (en) 2020-04-14
EA201291332A1 (ru) 2013-05-30
WO2011163632A3 (en) 2012-05-18
AR081303A1 (es) 2012-08-01
CL2016000905A1 (es) 2016-12-09
CL2012003665A1 (es) 2013-08-09
MX347113B (es) 2017-04-12
CN105755017A (zh) 2016-07-13
JP6301381B2 (ja) 2018-03-28
CL2016000904A1 (es) 2016-12-09
CA2999335C (en) 2021-10-19
MX350491B (es) 2017-09-07
KR20180008885A (ko) 2018-01-24
NZ700897A (en) 2015-10-30
MX2012015301A (es) 2014-04-25
ES2620492T3 (es) 2017-06-28
CA2803098A1 (en) 2011-12-29
WO2011163632A2 (en) 2011-12-29
KR20130098282A (ko) 2013-09-04
EP2862930B1 (en) 2017-02-08
ZA201209083B (en) 2014-02-26
IL223438A (en) 2016-10-31
AU2011270652A1 (en) 2013-01-10
JP2013534826A (ja) 2013-09-09
TW201634693A (zh) 2016-10-01
NZ700902A (en) 2016-04-29
EA029904B1 (ru) 2018-05-31
TW201634694A (zh) 2016-10-01
CN105734067A (zh) 2016-07-06
KR20180008884A (ko) 2018-01-24
NZ603971A (en) 2015-06-26
JP6096114B2 (ja) 2017-03-15
EP2862931A1 (en) 2015-04-22
CL2016000902A1 (es) 2016-10-14
IL244002A0 (en) 2016-04-21
CN103068986A (zh) 2013-04-24
CA2999335A1 (en) 2011-12-29
TW201634695A (zh) 2016-10-01
JP2016171789A (ja) 2016-09-29
EP2585600A4 (en) 2013-12-25

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