EP1568364B1 - Osteogenesis promoter containing beta-cryptoxanthin as the active ingredient - Google Patents

Osteogenesis promoter containing beta-cryptoxanthin as the active ingredient Download PDF

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EP1568364B1
EP1568364B1 EP03758826A EP03758826A EP1568364B1 EP 1568364 B1 EP1568364 B1 EP 1568364B1 EP 03758826 A EP03758826 A EP 03758826A EP 03758826 A EP03758826 A EP 03758826A EP 1568364 B1 EP1568364 B1 EP 1568364B1
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cryptoxanthin
bone
tissue
osteoporosis
results
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French (fr)
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EP1568364A1 (en
EP1568364A4 (en
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Masayoshi Yamaguchi
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Kemin Foods LC
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Kemin Foods LC
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/07—Retinol compounds, e.g. vitamin A
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis

Definitions

  • the present invention relates to ⁇ -Cryptoxanthin for use in the treatment of bone diseases selected from bone fracture, osteomalacia, osteopenia, osteoporosis, back pain or low back pain and to functional foods or food materials containing ⁇ -cryptoxanthin for use in preventing or treating such bone diseases, back pain or low back pain.
  • osteoporosis has a pathology caused by the following reasons: The bone mass is decreased as the balance of the bone resorption and the bone formation is lost by aging and, accordingly, the bone resorption is relatively increased to reduce the bone mass. As a result, the bone strength is decreased by the change in the fine structure of the bones to easily cause the fracture. Particularly in females, the bone mass is rapidly decreased after menopause, oophorectomy, etc.
  • Osteoporosis not only causes the fractures or sharp pain but it also makes the patients bedridden, particularly in cases of elderly people Under these circumstances, an effective cure is demanded for improving the quality of life in an aging society. Because it is difficult to cure patients with osteoporosis after the onset, the following points are now fully recognized: It is important to prevent this disease and it is also indispensable to start increasing of the amount of the bones in juvenile period. In addition, it is essential that nutrients required for the formation of bones and foods accelerating the formation of them must be taken everyday. As foods for strengthening the bones, calcium, magnesium and vitamin D are mainly taken nowadays. Casein phosphopeptide or the like for accelerating the absorption of calcium through the intestinal tracts is also used.
  • osteoporosis active vitamin D 3 , a female sex hormone (estrogen), calcitonin and ipriflavones are clinically used.
  • estrogen a female sex hormone
  • calcitonin calcitonin
  • ipriflavones a medicine for osteoporosis having an effect of polyisoprenoid derivatives typified by vitamin K 2 for inhibiting the formation of osteoclasts was developed (Japanese Patent Kokai No. Hei 7-215849 ).
  • Hei 10-279492 a remedy for bone diseases containing zinc acexamate as the active ingredient (Japanese Patent Kokai No. 10-218767 ); a composition for accelerating the bone formation and preventing the reduction in bone mineral density, which contains isoflavon as the main active ingredient (Japanese Patent Kokai No. Hei 10-114653 ); and an anti-osteoporotic composition containing reinforced vitamin K 2 and zinc (Japanese Patent Kokai No. Hei 10-36256 ).
  • WO00/06141 discloses a method of accentuating the effects of estrogen in a mammal comprising the step of administering an effective amount of xanthophyll, in order to delay bone demineralization.
  • ⁇ -cryptoxanthin (molecular weight: 552) is known as a carotenoid soluble in ethanol.
  • ⁇ -Cryptoxanthin is contained in citrus fruits, particularly in Satsuma oranges in an amount of 1 to 2 mg in each orange.
  • ⁇ -cryptoxanthin has characteristic properties of provitamin A.
  • ⁇ -cryptoxanthin has an anticancer effect stronger than that of ⁇ -carotene which is a carotenoid contained in green and yellow vegetables such as carrots and, therefore, ⁇ -cryptoxanthin is becoming the center of attention ( Biol. Pharm. Bull. 18, 2, 227, 1995 ).
  • ⁇ -cryptoxanthin is thus an important anti-carcinogenic component
  • techniques are now being developed for producing citrus fruits of a high quality having a ⁇ -cryptoxanthin content equal to that of the Satsuma oranges, for isolating genes for synthesizing ⁇ -cryptoxanthin (Japanese Patent Kokai Nos. Hei 11-155577 and Hei 11-46770 ), and for isolating a large amount of ⁇ -cryptoxanthin from the citrus fruits for the purpose of developing citrus fruits having an increased ⁇ -cryptoxanthin content and processed citrus fruit foods.
  • a method for producing ⁇ -cryptoxanthin of a high purity which comprises the steps of pressing orange juice, obtaining an extract containing ⁇ -cryptoxanthin from the resultant precipitate with a solvent, hydrolyzing the extract, introducing the hydralyzate into the first column filled with silica powder having an average particle diameter of 10 to 80 ⁇ m together with a primary development solvent at a linear velocity of at least 2 cm/min to separate a fraction containing ⁇ -cryptoxanthin, removing the solvent, and introducing the isolated product into the second column filled with octadecylsilane silica having an average particle diameter of 10 to 80 ⁇ m together with a secondary development solvent at a linear velocity of at least 2 cm/min to separate the fraction containing at least 95 % by weight of ⁇ -cryptoxanthin (Japanese Patent Kokai No.
  • a process for producing pulps having a high carotenoid content which comprises the steps of squeezing citrus fruits, filtering or sieving the obtained juice, centrifuging the obtained the juice, adding an enzyme to the obtained precipitate, freezing the resultant mixture, thawing the mixture and dehydrating the obtained product; and a method for producing pulp containing an increased amount of carotenoid, ⁇ -cryptoxanthin, etc. and powder thereof which comprises the steps of repeating a process of adding water to a pulp having a high carotenoid content and dehydrating the pulp, and then drying and pulverizing the pulp (Japanese Patent Kokai No. 2000-23637 ).
  • a method for producing chemically possible combinatorial products from a large data base of finger prints having 3D multiple stereostructure and screening the products which comprises the steps of temporarily binding the radical with a bulky space keeping group, registering the 3D model of the radical in a combinatorial ghost data base, detecting an optional atom having characteristic physical properties of pharmacophore type for an accessible optional molecular structure in the ghost data base; calculating all the distances between atoms relating to the whole stereostructure of the molecule, for the pair of the pharmacophare detected in each molecular structure to prepare the distance distribution density; preparing a stereostructure finger print vector including all the distance distribution density of the pair of the pharmacophore; defining the gauge function for each finger print for explaining the relative importance of the characteristics of the pharmacophore; preparing the finger print of the lead compound, comparing the finger print with each finger print of a passible library in the gauge function for making the lead compound maximum, and retrieving
  • the object of the present invention is to provide an osteogenesis promoter having a remarkable effect of positively promoting osteogenesis and thus preventing / treating bone diseases, a preventive / a remedy for bone diseases such as osteoporosis having both of an osteogenesis-promoting effect and a bone resorption-inhibiting effect, and a method of screening an active ingredient for preventing / treating bone diseases by using a compound having both of an osteogenesis-promoting effect and a bone resorption-inhibiting effect as a lead compound.
  • ⁇ -cryptoxanthin which is contained in a large amount in peel and sarcocarp of Satsuma orange, has an osteogenesis-promoting effect and effect of preventing / treating bone diseases.
  • the inventors made experiments wherein diaphysis and metaphysis tissue of a femur were cultured in a culture medium containing ⁇ -cryptoxanthin, then calcium level in the bone tissue, the amount of the expressed bone calcification accelerating enzyme and bone DNA level which is the index of the number of the cells in the bone tissue were determined to confirm a significant increase in all the cases.
  • ⁇ -cryptoxanthin accelerates the synthesis of protein in the cancellous bone in the femur tissue (tissue of the metaphysis) and cortical bone (tissue of the diaphysis) to promote the osteogenesis.
  • the inventors cultured a bone tissue in the presence of both parathyroid hormone (PTH), having an effect of dissolving the bone mineral (bone resorption) and a physiological role of causing pathology of osteoporosis due to aging, and ⁇ -cryptoxanthin to confirm that the reduction in amount of calcium in the tissue of the diaphysis and tissue of the metaphysic can be significantly controlled.
  • PTH parathyroid hormone
  • the present invention relates to ⁇ -cryptoxanthin for use in the treatment of bone diseases selected from bone fracture, osteomalacia, osteopenia, osteoporosis, back pain or lower back pain (claim 1), ⁇ -cryptoxanthin for use according to claim 1 in the form of a ⁇ -cryptoxanthin-containing composition (claim 2) and which is obtainable by treating Satsuma orange (claim 3), functional foods or food materials for use in preventing or treating bone diseases, selected from bone fracture, osteomalacia, osteopenia, osteoporosis, back pain or low back pain which contain ⁇ -cryptoxanthin (claim 8), functional foods or food materials for such uses which contain a ⁇ -cryptoxanthin-containing composition (claim 9), and which are obtainable by treating Satsuma orange (claim 10) and such functional foods or food materials for use in preventing or treating osteoporosis (claim 11).
  • the osteogenesis promoter of the present invention is not particularly limited so far as it contains ⁇ -cryptoxanthin or a ⁇ - cryptoxanthin-containing composition as the active ingredient.
  • the Preventive/ remedy for bone diseases of the present invention is also not particularly limited so far as it contains ⁇ -cryptoxanthin or a ⁇ -cryptoxanthin-containing composition as the active ingredient.
  • the functional foods or food materials for preventing or treating bone diseases of the present invention are not particularly limited so far as they are foods or food materials containing ⁇ -cryptoxanthin or a ⁇ -cryptoxanthin-containing composition and having the function of preventing or treating bone diseases and usable for preventing or treating the bone diseases.
  • the feed composition of the present invention is also not particularly limited so far as it contains ⁇ -cryptoxanthin or a ⁇ -cryptoxanthin-containing composition.
  • the bone diseases are bone fractures, osteomalacia, osteopenia, osteoporosis and back pain and low back pain.
  • examples of the bone diseases for which the osteogenesis promoter is particularly recommended are osteoporosis such as postmenopausal osteoporosis, estrogen-deficiency osteoporosis, senile osteoporosis and steroid-induced osteoporosis, as well as metabolic bone diseases such as osteomalacia.
  • a method of obtaining ⁇ -cryptoxanthin or a ⁇ -cryptoxanthin containing composition is not particularly limited and a known method such as a method wherein it is extracted / produced from citrus fruits or a method wherein a gene encoding a ⁇ -cryptoxanthin-producing enzyme is utilized is employed.
  • Preferred starting materials for ⁇ -cryptoxanthin and the ⁇ -cryptoxanthin-containing composition are Satsuma oranges containing ⁇ -cryptoxanthin in an amount of 1 to 2 mg/orange which is at least 60 times as much as ⁇ -cryptoxanthin content of other citrus oranges such as other oranges, grape fruits and lemons.
  • particularly preferred oranges are those of varieties having a high ⁇ -cryptoxanthin content such as Sugiyama oranges containing about 8 mg of ⁇ -cryptoxanthin per 100 g of the peel (flavedo) and about 1 mg of ⁇ -cryptoxanthin per 100 mg of the juice thereof and oranges obtained by the cross-fertilization with Satsuma oranges.
  • the term " ⁇ -cryptoxanthin-containing composition" herein indicates a composition having an artificially increased ⁇ -cryptoxanthin content.
  • a method of processing Satsuma oranges to obtain the ⁇ -cryptoxanthin-containing composition by processing Satsuma oranges is not limited. For example, methods described in the above-described patent specifications 10 and 11 and methods described in non-patent literatures 2 to 4 can be employed.
  • ⁇ -cryptoxanthin or the ⁇ -cryptoxanthin-containing composition is used as the medicine for preventing / treating the bone diseases
  • various components for the prescription can be used. They are pharmaceutically acceptable ordinary components such as a carrier, binder, stabilizer, excipient, diluent, pH buffer, disintegrator, solubilizer and isotonizing agent.
  • a carrier such as a carrier, binder, stabilizer, excipient, diluent, pH buffer, disintegrator, solubilizer and isotonizing agent.
  • the above-described, well-known substances having the effect of accelerating the osteogenesis and/or inhibiting the bone resorption, and minerals such as calcium, magnesium and phosphorus can also be used together with them.
  • These preventive agents or remedies can be orally or parenterally administered.
  • they can be orally administered in an ordinary administration form such as a powder, granules, capsules, a syrup or a suspension and also they can be parenterally administered in the form of, for example, a solution, an emulsion or a suspension by the injection, or they can be administered in the form of a spray to the nostrils.
  • the oral administration is preferred.
  • the dose can be suitably determined depending on the purpose of the administration (prevention or treatment), kind and seriousness of the bone disease and age of the patient.
  • the kinds of foods and food materials containing ⁇ -cryptoxanthin or a ⁇ -cryptoxanthin-containing composition and having the function of preventing or treating bone diseases, which are to be used for the prevention or treatment of bone diseases are not particularly limited.
  • the foods and food materials include drinks such as yogurt, yogurt drink, juices, cow's milk, soybean milk, liquors, coffee, black tea, green tea, oolong tea and sport drinks; baked cakes such as puddings, cookies, breads, cakes, jellies and rice crackers; Japanese cakes such as sweetened and jellied bean pastes; breads and cakes such as frozen sweets and chewing gums; noodles such as thick white noodles and buckwheat noodles; fish paste products such as boiled fish pastes, fish hams and fish sausages; seasonings such as miso (fermented soybean paste), soy sauce, dressings, mayonnaise and sweetening agents; milk products such as cheeses and butters; and various side dishes such as bean curds, konnyaku (a gelatinous food made
  • the feed composition containing ⁇ -cryptoxanthin or that containing the ⁇ -cryptoxanthin-containing composition is advantageously usable for growing domestic animals and poultry such as pigs, cattle and chickens; pets such as dogs and cats; and farmed fish and shellfish.
  • a feed composition may also contain the above-described well-known substances having the osteogenesis-accelerating effect and/or bone resorption-inhibiting effect such as ipriflavones as well as minerals such as calcium, magnesium, phosphorous, iron, zinc, manganese and copper.
  • Active ingredients for the preventive/remedy for bone diseases such as osteoporosis may be screened by a screening method wherein ⁇ -cryptoxanthin is used as the lead compound.
  • the screening method wherein ⁇ -cryptoxanthin is used as the lead compound the development of a more effective osteogenesis promoter or a preventive /remedy for bone diseases is made possible.
  • a combinatorial chemistry technique such as a method stated in the above-described patent literature 12 can be used. Even when the combinatorial chemistry technique is not used, the screening of the osteogenesis promoter or the preventive / remedy for bone diseases with ⁇ -cryptoxanthin as the lead compound can be conducted by a classical technique structural activity correlation technique.
  • the femurs were extracted from rats (Wistar male rats; 4 to 5 weeks old) (purchased from Japan SLC Co., Ltd. and fed with solid Oriental yeast (MF)) under anesthesia with ether under a germfree condition.
  • the femurs were each washed with 0.25 M sucrose solution, and then divided into the diaphysis (cortical bone) and metaphysis (cancellous bone).
  • the bone tissue pieces were cultured in a culture medium (Dulbecco's modified culture medium containing 5 % of glucose; serum-free medium) containing ⁇ -cryptoxanthin, etc. at 37°C in an incubator filled with 5 % CO 2 - 95 % air for 48 hours.
  • ⁇ -cryptoxanthin used was " ⁇ -cryptoxanthin” of Extrasynthase Co.
  • cycloheximide used was “Cycloheximide” of Sigma Co. Ltd.
  • ⁇ -carotene was “ ⁇ -carotene” of Sigma Co. Ltd.
  • xanthine was “Xanthine” of Sigma Co. Ltd.
  • parathormone was “Parathormone” of Sigma Co. Ltd.
  • prostaglandin E 2 was "Prostaglandin E 2 " of Sigma Co. Ltd..
  • In the control only the culture medium was used without ⁇ -cryptoxanthin or the like.
  • Bone calcium level in the bone tissue was determined. After the culture in the incubator, the tissue pieces were washed with 0.25 M sucrose solution and dried and then the bone was weighed. Then concentrated nitric acid was added to the tissue pieces. After the incineration at 120°C for 12 hours, the bone calcium level was determined with an atomic absorption photometer ("Perkin-Elmer 303" of Perkin-Elmer Co.).
  • alkaline phosphatase which is the most important enzyme for the acceleration of the calcification of bones.
  • the tissue pieces cultured in the incubator' were washed in 0.25 M sucrose solution, then pulverized in 3 ml of 6 5 mM barbital buffer (pH 7.4) and treated with ultrasonic waves.
  • the resultant liquid was centrifuged.
  • the supernatant, as the enzyme solution was determined by a method of Walter ad Schutt in Method of Enzymatic Analysis, Vol. 1-2, p. 856, Academic Press, New York, 1965 ). Namely, this method was carried out as follows: p-nitrophenylphosphoric acid was used as the substrate.
  • DNA level was determined as an index of the number of cells in the bone tissue. After the culture in an incubator, the tissue pieces were washed with 0.25 M sucrose solution and then the wet weight of them was determined. They were pulverized in 4 ml of 0.1 N NaOH. After the osmosis at 4°C for 24 hours, the liquid mixture was centrifuged. The supernatant was taken as the sample and determined by a method of Ceriotti at al. (J. _Biol. Chem., 241; 34-77, 1951 ). Namely, 1 ml of concentrated hydrochloric acid and 1 ml of 0.04 % indole solution were added to 2 ml of the sample and then the resultant mixture was heated to 100°C in boiling water. After quenching followed by the extraction with 4 ml of chloroform, the chloroform layer was taken to determine bone DNA level with a spectrophotometer (490 nm).
  • a solution of ⁇ -cryptoxanthin in corn oil having one of three kinds of concentration (10, 25 and 50 ⁇ g/ml corn oil) was orally administered to rats (Wistar male rats; 4 to 5 weeks old) in an amount /100 g body weight with a probe once a day for 7 days.
  • the rats were sacrificed 24 hours after the final administration.
  • the femurs were excised.
  • the muscles, etc. were taken out and each femur was divided into diaphysis and metaphysis to determine the bone components.
  • the bone component-increasing effect of ⁇ -cryptoxanthin was examined.
  • the diaphysis and metaphysis tissue of the femur was cultured in the above-described culture medium containing ⁇ -cryptoxanthin (10 -8 to 10 -5 M) for 48 hours.
  • Calcium level in the bone tissue, alkaline phosphatase activity (enzyme for accelerating the bone calcification) and the amount of deoxyribonucleic acid (DNA; index of number of cells in the bone tissue) were determined by the same methods as in Example 1. The results are shown in Table 1 and Figs. 3 to 6 . In each test group, the determination was conducted 6 to 8 times and the results were represented by the average value and the standard error.
  • a protein synthesis inhibitor that reacts on 60S liposome of eukaryotic cells and inhibits the transition reaction in the peptide chain elongation was used.
  • Calcium level in the bone tissue, alkaline phosphatase activity and DNA level were determined in the presence of ⁇ -cryptoxanthin (10 -6 M) or cycloheximide (10 -6 M) or in the coexistence of ⁇ -cryptoxantlain (10 -6 M) and cycloheximide (10 -6 M). The results are shown in Table 2 and Figs. 7 to 12 .
  • Parathyroid hormone is a peptide hormone secreted from the accessory thyroid to exhibit the effect of the bone mineral dissolution (bone resorption). PTH also has a pathophysiologic role on the expression of osteoporosis caused by the aging. It is known that prostaglandin E 2 also causes physiologic bone mineral dissolution.
  • the diaphysis or metaphysic tissue of the femur was cultured in the presence of 10 -7 M PTH or 10 -6 M prostaglandin E 2 for 48 hours and then calcium level in the bone tissue was determined.
  • ⁇ -cryptoxanthin increases and accelerates the bone formation and also inhibits the bone resorption to exhibit the effect of retaining and increasing the amount of bone mineral and thus it functions as an anti-osteoporotic factor.
  • ⁇ -cryptoxanthin was administered for 7 days (10, 25 or 50 ⁇ g/100 g body weight/day) and then calcium level in the bone tissue, determination of alkaline phosphatase activity (enzyme for the acceleration of the calcification of bones) and the amount of deoxyribonucleic acid (DNA: index of the number of the cells in the bone tissue) were determined by the same methods as those in Example. The results are shown in Table 5 and Figs. 19 to 22 . In each test group, the determination was conducted 6 times and the results were represented by the average value and the standard error. The significant difference was determined by Student's t-test.
  • the alkali phosphatase activity (enzyme for the acceleration of the calcification of bones) in the diaphysis and metaphysis tissue was significantly increased by the administration of ⁇ -cryptoxanthin (10, 25 or 50 ⁇ g/100 g body weight/day).
  • DNA in the bone tissue was significantly increased by the administration of ⁇ -cryptoxanthin (25 or 50 ⁇ g/100 g body weight/day) in the diaphysis, and it also significantly increased by the administration of ⁇ -cryptoxanthin (10, 25 or 50 ⁇ g/100 g body weight/day) in the metaphysis
  • ⁇ -cryptoxanthin is considered to be useful as an osteogenesis promoter and effective in preventing and treating osteoporosis.
  • Diaphysis tissue Control ⁇ -cryptoxanthin ( ⁇ g/100g body weight/day 10 25 50 Bone calcium level (mg/g dry weight) 205.1 ⁇ 5.40 232.8 ⁇ 3.00* 248.3 ⁇ 5.10* 247.5 ⁇ 4.20* Bone alkaline phosphatase activity ⁇ mol/min/mg protein) 1.02 ⁇ 0.010 2.38 ⁇ 0.019* 2.35 ⁇ 0.021* 1.85 ⁇ 0.025* Bone DNA level - (mg/g bone wet weight) 1.75 ⁇ 0.11 1.95 ⁇ 0.18 3.01 ⁇ 0.23* 2.53 ⁇ 0.20* Metaphysis tissue Control ⁇ -cryptoxanthin ( ⁇ g/100 g body weight/day) 10 25 50 Bone calcium level (mg/g dry weight) 168 3 ⁇ 4.00 201.5 ⁇ 5.60* 200.3 ⁇ 3.90* 205.9 ⁇ 4.10* Bone alkaline phosphatase ( ⁇ mol/min/mg protein) 1 28 ⁇ 0.013 2.21 ⁇ 0.023* 2.545*
  • the present invention can provide an osteogenesis promoter containing ⁇ -cryptoxanthin as the active ingredient and having a remarkable effect of accelerating the osteogenesis to prevent and treat bone diseases and also agents having both osteogenesis promoting effect and bone resorption inhibiting effect and usable for preventing and treating bone diseases such as osteoporosis

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EP03758826A 2002-10-25 2003-10-23 Osteogenesis promoter containing beta-cryptoxanthin as the active ingredient Expired - Lifetime EP1568364B1 (en)

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JP2002311930 2002-10-25
PCT/JP2003/013561 WO2004037236A1 (ja) 2002-10-25 2003-10-23 β−クリプトキサンチンを有効成分とする骨形成促進剤

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EP1568364A4 EP1568364A4 (en) 2009-01-07
EP1568364B1 true EP1568364B1 (en) 2013-03-27

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US20080070980A1 (en) * 2004-05-18 2008-03-20 Anne Eichinger Use Of Beta-Cryptoxanthin
JP2006104090A (ja) * 2004-10-01 2006-04-20 Unitika Ltd 抗骨粗鬆症組成物
CA2596417A1 (en) * 2004-11-16 2006-05-26 Masayoshi Yamaguchi Composition for promoting osteogenesis and increasing bone mineral content
JP5099617B2 (ja) * 2006-03-16 2012-12-19 国立大学法人東京農工大学 歯周病予防・治療剤
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JPWO2004037236A1 (ja) 2006-02-23
US20060106115A1 (en) 2006-05-18
EP1568364A1 (en) 2005-08-31
JP3892014B2 (ja) 2007-03-14
CA2503557C (en) 2013-04-02
CA2503557A1 (en) 2004-05-06
EP1568364A4 (en) 2009-01-07
US8148431B2 (en) 2012-04-03

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