EP0707658A1 - In flüssiger form stabiler thiolaktivator - Google Patents

In flüssiger form stabiler thiolaktivator

Info

Publication number
EP0707658A1
EP0707658A1 EP95918415A EP95918415A EP0707658A1 EP 0707658 A1 EP0707658 A1 EP 0707658A1 EP 95918415 A EP95918415 A EP 95918415A EP 95918415 A EP95918415 A EP 95918415A EP 0707658 A1 EP0707658 A1 EP 0707658A1
Authority
EP
European Patent Office
Prior art keywords
thiol
solution
liquid
composition
activator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP95918415A
Other languages
English (en)
French (fr)
Other versions
EP0707658B1 (de
Inventor
Carrie J. Lu
Frederick S. Yein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Publication of EP0707658A1 publication Critical patent/EP0707658A1/de
Application granted granted Critical
Publication of EP0707658B1 publication Critical patent/EP0707658B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase

Definitions

  • a biological molecule in a sample of a physiological fluid can be detected by detecting the activity, influence or effect of the biological molecule upon a substance in its environment, such as a substrate in solution with the biological molecule.
  • a test sample of a physiological fluid is removed from an in vivo environment into a non-biophysiological, in vitro environment, such as by drawing a sample of a patient's physiological fluid into a test tube, the activity of many biological molecules in the test sample will rapidly decline.
  • an in vivo equivalent of the normal activity of a biological molecule it is necessary to reactivate the biological molecule prior to or at the time of the in vitro assay for biological molecule activity.
  • Many different thiol compounds have been used to reactivate diverse biological molecules of interest.
  • the creatine kinase assay is generally carried out using a spectrophoto eter to measure the change in light absorption at a wavelength of about 340 nm 4 due to formation of reduced form ⁇ - nicotinamideadenine dinucleotide (phosphate) during the following reactions:
  • the free radical scavenger used as a stabilizing agent can be used in' particular to stabilize the thiol present in a creatine kinase reagent.
  • the creatine kinase reagent is used to reactivate the creatine kinase present in a sample of a physiological fluid.
  • the creatine kinase assay detects reactivated creatine kinase catalytic activity through a spectrophotometric light absorption method.
  • a significant aspect of the present invention is the discovery that the thiol activator present in the liquid of the composition can retain its' activation ability substantially undiminished for at least about one year, when the composition is stored at about 4° C. in an air tight bottle or other container, protected from the light.
  • the thiol activator in the composition of our invention is liquid stable under such storage conditions for as long as about two or three years.
  • Table 2 shows the results of the 41° C. stress test.
  • EDTA was absent (i.e. creatine kinase buffer solutions C, D, G and H)
  • the NAC present in the creatine kinase buffer solution rapidly became unstable.
  • the presence of EDTA in the creatine kinase buffer solution as expected, delayed significant NAC deterioration until about five days after buffer preparation.
  • aqueous NAC stability improved significantly when 2.0 mmol/L of EDTA was present in the creatine kinase buffer solution.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Medical Preparation Storing Or Oral Administration Devices (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
EP95918415A 1994-05-06 1995-05-05 In flüssiger form stabiler thiolaktivator Expired - Lifetime EP0707658B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23926094A 1994-05-06 1994-05-06
US239260 1994-05-06
PCT/US1995/005702 WO1995030769A1 (en) 1994-05-06 1995-05-05 Liquid stable thiol activator

Publications (2)

Publication Number Publication Date
EP0707658A1 true EP0707658A1 (de) 1996-04-24
EP0707658B1 EP0707658B1 (de) 2002-08-07

Family

ID=22901361

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95918415A Expired - Lifetime EP0707658B1 (de) 1994-05-06 1995-05-05 In flüssiger form stabiler thiolaktivator

Country Status (9)

Country Link
US (1) US5700653A (de)
EP (1) EP0707658B1 (de)
JP (1) JP3619865B2 (de)
AT (1) ATE221919T1 (de)
AU (1) AU692547B2 (de)
CA (1) CA2165808A1 (de)
DE (1) DE69527686T2 (de)
ES (1) ES2180632T3 (de)
WO (1) WO1995030769A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19755079A1 (de) * 1997-12-11 1999-06-17 Roche Diagnostics Gmbh Stabilisiertes Reagenz und Verfahren zur Bestimmung von Creatin-Kinase
US5908864A (en) * 1998-05-28 1999-06-01 Dymatize Enterprises Creatine gel
ES2272037T3 (es) 1998-12-22 2007-04-16 Olympus Life And Material Science Europa Gmbh Reactivo liquido para la deteccion de creatina cinasa.
AU2404500A (en) * 1999-01-08 2000-07-24 Medical Analysis Systems, Inc. Compositions and methods for stabilizing thiol-containing biomolecules
US20060020035A1 (en) * 2004-03-11 2006-01-26 Oregon Health & Science University Bone marrow protection with N-acetyl-L-cysteine
AU2011202871B2 (en) * 2005-08-24 2014-06-12 Cumberland Pharmaceuticals Inc. Acetylcysteine composition and uses therefor
US8148356B2 (en) * 2005-08-24 2012-04-03 Cumberland Pharmaceuticals, Inc. Acetylcysteine composition and uses therefor
JP5468299B2 (ja) * 2009-05-15 2014-04-09 旭化成ファーマ株式会社 Ｉｍｐｄｈ含有組成物及びｉｍｐｄｈの安定化方法
WO2011112900A2 (en) * 2010-03-12 2011-09-15 Cytotech Labs, Llc Intravenous formulations of coenzyme q10 (coq10) and methods of use thereof
EA201490047A1 (ru) 2011-06-17 2014-08-29 Берг Ллк Ингаляционные фармацевтические композиции

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2648759C3 (de) * 1976-10-27 1979-05-31 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisierungsmittel für Enzyme
US4339533A (en) * 1980-07-30 1982-07-13 Technicon Instruments Corporation Stabilization of creatine kinase (CK) and its application as a reference standard
US4883762A (en) * 1983-06-06 1989-11-28 Ciba Corning Diagnostics Corp. Stabilized isoenzyme control products
JPS6188899A (ja) * 1984-10-05 1986-05-07 Unitika Ltd クレアチンキナ−ゼ定量用試薬
JPS62104598A (ja) * 1985-11-01 1987-05-15 Yatoron:Kk クレアチンキナ−ゼ測定用試薬
FR2610626B1 (fr) * 1987-02-09 1989-05-19 Oreal Nouveau systeme anti-oxydant a base d'un ester d'ascorbyle stabilise, contenant en association au moins un agent complexant et au moins un thiol, et compositions contenant un tel systeme anti-oxydant
JPS63283600A (ja) * 1987-05-18 1988-11-21 Fuji Photo Film Co Ltd クレアチンキナ−ゼ活性測定用分析要素
JPH0690202B2 (ja) * 1988-04-06 1994-11-14 ユニチカ株式会社 クレアチンキナーゼ活性測定用試薬およびそれを用いる測定方法
CA2028593C (en) * 1989-10-31 2007-08-21 Douglas R. Brandt Stabilized enzyme compositions
IT1242642B (it) * 1990-04-17 1994-05-16 Alfa Wassermann Spa Formulazioni iniettabili contenenti naproxen sale sodico.
US5192657A (en) * 1990-12-18 1993-03-09 Ortho Diagnostic Systems, Inc. Stabilized proteolytic solution and reagent kit
JPH05111396A (ja) * 1991-07-17 1993-05-07 Oriental Yeast Co Ltd クレアチンキナ−ゼの活性測定方法 およびそれに用いる試薬
US5298406A (en) * 1992-09-14 1994-03-29 E. I. Du Pont De Nemours And Company Formulation for stabilizing enzymatic activity and immunoreactivity of creatine kinase and creatine kinase isoenzymes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9530769A1 *

Also Published As

Publication number Publication date
JP3619865B2 (ja) 2005-02-16
WO1995030769A1 (en) 1995-11-16
EP0707658B1 (de) 2002-08-07
ES2180632T3 (es) 2003-02-16
ATE221919T1 (de) 2002-08-15
AU2436795A (en) 1995-11-29
DE69527686T2 (de) 2003-04-03
JPH09500027A (ja) 1997-01-07
CA2165808A1 (en) 1995-11-16
US5700653A (en) 1997-12-23
AU692547B2 (en) 1998-06-11
DE69527686D1 (de) 2002-09-12

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