EE04224B1 - Gaasi mikromullide dispersioon ja selle lüofiliseeritud jääk, ultraheli kontrastaine ja selle valmistamismeetod, mikromulle eraldav maatriks ning ultraheli kontrastaine kompositsioon - Google Patents

Gaasi mikromullide dispersioon ja selle lüofiliseeritud jääk, ultraheli kontrastaine ja selle valmistamismeetod, mikromulle eraldav maatriks ning ultraheli kontrastaine kompositsioon

Info

Publication number
EE04224B1
EE04224B1 EE9800249A EE9800249A EE04224B1 EE 04224 B1 EE04224 B1 EE 04224B1 EE 9800249 A EE9800249 A EE 9800249A EE 9800249 A EE9800249 A EE 9800249A EE 04224 B1 EE04224 B1 EE 04224B1
Authority
EE
Estonia
Prior art keywords
contrast medium
ultrasonic contrast
microbubbles
dispersion
matrix
Prior art date
Application number
EE9800249A
Other languages
English (en)
Estonian (et)
Other versions
EE9800249A (et
Inventor
Undheim Br�nden Jorunn
Dugstad Harald
Klaveness Jo
Rongved P�l
Skurtveit Roald
Original Assignee
Nycomed Imaging As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9603466.5A external-priority patent/GB9603466D0/en
Priority claimed from GBGB9611894.8A external-priority patent/GB9611894D0/en
Priority claimed from GBGB9625663.1A external-priority patent/GB9625663D0/en
Application filed by Nycomed Imaging As filed Critical Nycomed Imaging As
Publication of EE9800249A publication Critical patent/EE9800249A/xx
Publication of EE04224B1 publication Critical patent/EE04224B1/xx

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00Preparations for testing in vivo
    • A61K49/22Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00Preparations for testing in vivo
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00Preparations for testing in vivo
    • A61K49/22Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations
    • A61K49/222Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations characterised by a special physical form, e.g. emulsions, liposomes
    • A61K49/223Microbubbles, hollow microspheres, free gas bubbles, gas microspheres
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00Preparations for testing in vivo
    • A61K49/22Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations
    • A61K49/222Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations characterised by a special physical form, e.g. emulsions, liposomes
    • A61K49/227Liposomes, lipoprotein vesicles, e.g. LDL or HDL lipoproteins, micelles, e.g. phospholipidic or polymeric
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00Stock material or miscellaneous articles
    • Y10T428/29Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982Particulate matter [e.g., sphere, flake, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Acoustics & Sound (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicinal Preparation (AREA)
EE9800249A 1996-02-19 1997-02-19 Gaasi mikromullide dispersioon ja selle lüofiliseeritud jääk, ultraheli kontrastaine ja selle valmistamismeetod, mikromulle eraldav maatriks ning ultraheli kontrastaine kompositsioon EE04224B1 (et)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9603466.5A GB9603466D0 (en) 1996-02-19 1996-02-19 Improvements in or relating to contrast agents
GBGB9611894.8A GB9611894D0 (en) 1996-06-07 1996-06-07 Improvements in or relating to contrast agents
GBGB9625663.1A GB9625663D0 (en) 1996-12-11 1996-12-11 Improvements in or relating to contrast agents
PCT/GB1997/000459 WO1997029783A1 (en) 1996-02-19 1997-02-19 Improvements in or relating to contrast agents

Publications (2)

Publication Number Publication Date
EE9800249A EE9800249A (et) 1999-02-15
EE04224B1 true EE04224B1 (et) 2004-02-16

Family

ID=27268137

Family Applications (1)

Application Number Title Priority Date Filing Date
EE9800249A EE04224B1 (et) 1996-02-19 1997-02-19 Gaasi mikromullide dispersioon ja selle lüofiliseeritud jääk, ultraheli kontrastaine ja selle valmistamismeetod, mikromulle eraldav maatriks ning ultraheli kontrastaine kompositsioon

Country Status (24)

Country Link
US (4) US6221337B1 (Direct)
EP (2) EP1331013A3 (Direct)
JP (1) JP4418033B2 (Direct)
KR (1) KR100500334B1 (Direct)
CN (1) CN1278740C (Direct)
AP (1) AP890A (Direct)
AT (1) ATE238072T1 (Direct)
AU (1) AU726503B2 (Direct)
BG (1) BG102758A (Direct)
CA (1) CA2246779C (Direct)
CZ (1) CZ293986B6 (Direct)
DE (1) DE69721235T2 (Direct)
EA (1) EA001135B1 (Direct)
EE (1) EE04224B1 (Direct)
ES (1) ES2197986T3 (Direct)
HU (1) HU229090B1 (Direct)
IL (1) IL125748A (Direct)
NZ (1) NZ331509A (Direct)
OA (1) OA10839A (Direct)
PL (1) PL328406A1 (Direct)
PT (1) PT881915E (Direct)
SK (1) SK284200B6 (Direct)
TR (1) TR199801613T2 (Direct)
WO (1) WO1997029783A1 (Direct)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL128345A0 (en) 1996-08-02 2000-01-31 Nycomed Imaging As Improvements in or relating to contrast agents
AU784367B2 (en) * 1996-08-02 2006-03-16 Ge Healthcare As Improvements in or relating to contrast agents
JP4317270B2 (ja) * 1997-08-12 2009-08-19 ブラッコ・リサーチ・ソシエテ・アノニム 投与性組成物および磁気共鳴画像法
GB9717476D0 (en) * 1997-08-18 1997-10-22 Nycomed Imaging As Process
GB9717542D0 (en) * 1997-08-19 1997-10-22 Nycomed Imaging As Process
GB9717589D0 (en) * 1997-08-19 1997-10-22 Nycomed Imaging As Improvements in or relating to contrast agents
GB9717588D0 (en) * 1997-08-19 1997-10-22 Nycomed Imaging As Improvements in or relating to contrast agents
GB9726773D0 (en) 1997-12-18 1998-02-18 Nycomed Imaging As Improvements in or relating to ultrasonagraphy
ATE265525T1 (de) * 1998-04-28 2004-05-15 Amersham Health As Verbesserung von trennungsverfahren
GB9813568D0 (en) 1998-06-23 1998-08-19 Nycomed Imaging As Improvements in or relating to cardiac imaging
US6514209B1 (en) 1999-06-07 2003-02-04 Drexel University Method of enhancing ultrasonic techniques via measurement of ultraharmonic signals
US20030103960A1 (en) * 1999-12-22 2003-06-05 Pierre Philippart Sealant and bone generating product
CA2410887C (en) 2000-06-02 2012-07-24 Bracco Research Usa Compounds for targeting endothelial cells, compositions containing the same and methods for their use
NO20004795D0 (no) 2000-09-26 2000-09-26 Nycomed Imaging As Peptidbaserte forbindelser
US6984373B2 (en) 2000-12-23 2006-01-10 Dyax Corp. Fibrin binding moieties useful as imaging agents
KR100373712B1 (ko) * 2000-12-23 2003-02-25 사회복지법인삼성생명공익재단(삼성서울병원) 초음파 조영제 및 그의 제조방법
EP1387637B1 (en) 2001-04-06 2007-10-31 Bracco Research S.A. Apparatus for measuring local physical parameters in a fluid filled cavity
WO2003063824A2 (en) * 2002-02-01 2003-08-07 Shimoda Biotech (Pty) Ltd Pharmaceutical composition
WO2004065621A1 (en) 2002-03-01 2004-08-05 Dyax Corp. Kdr and vegf/kdr binding peptides and their use in diagnosis and therapy
US7261876B2 (en) 2002-03-01 2007-08-28 Bracco International Bv Multivalent constructs for therapeutic and diagnostic applications
US7794693B2 (en) 2002-03-01 2010-09-14 Bracco International B.V. Targeting vector-phospholipid conjugates
US7211240B2 (en) 2002-03-01 2007-05-01 Bracco International B.V. Multivalent constructs for therapeutic and diagnostic applications
EP2301587B1 (en) 2002-03-01 2014-06-25 Dyax Corp. KDR and VEGF/KDR binding peptides and their use in diagnosis
US8623822B2 (en) 2002-03-01 2014-01-07 Bracco Suisse Sa KDR and VEGF/KDR binding peptides and their use in diagnosis and therapy
NO20024755D0 (no) * 2002-10-03 2002-10-03 Amersham Health As Metode
KR20050072496A (ko) 2002-11-29 2005-07-11 아머샴 헬스 에이에스 초음파 촬영 방법
JP4670083B2 (ja) 2003-02-04 2011-04-13 ブラッコ・シュイス・ソシエテ・アノニム 超音波造影剤およびその製造方法
US20070128117A1 (en) * 2003-02-04 2007-06-07 Bracco International B.V. Ultrasound contrast agents and process for the preparation thereof
ES2557286T3 (es) 2003-03-03 2016-01-25 Dyax Corp. Usos de péptidos que se unen específicamente al receptor del HGF (cMet)
WO2005002546A1 (en) * 2003-06-27 2005-01-13 Smithkline Beecham Corporation Stabilized topotecan liposomal composition and methods
US20050019379A1 (en) 2003-07-22 2005-01-27 Kimberly-Clark Worldwide, Inc. Wipe and methods for improving skin health
NO20035401D0 (no) * 2003-12-04 2003-12-04 Amersham Health As Metode
US20070081946A1 (en) * 2003-12-22 2007-04-12 Bracco Research S.A. Assembly of gas-filled microvesicle with active component for contrast imaging
EP1701745B1 (en) 2003-12-22 2014-12-10 Bracco Suisse S.A. Gas-filled microvesicle assembly for contrast imaging
US7025726B2 (en) 2004-01-22 2006-04-11 The Regents Of The University Of Nebraska Detection of endothelial dysfunction by ultrasonic imaging
AU2005273865B2 (en) 2004-08-18 2011-02-24 Bracco Suisse S.A. Gas-filled microvesicles composition for contrast imaging
WO2006044996A2 (en) * 2004-10-15 2006-04-27 The Trustees Of Columbia University In The City Of New York System and method for automated boundary detection of body structures
WO2006044997A2 (en) * 2004-10-15 2006-04-27 The Trustees Of Columbia University In The City Of New York System and method for localized measurement and imaging of viscosity of tissues
CN100574811C (zh) * 2005-01-10 2009-12-30 重庆海扶(Hifu)技术有限公司 一种高强度聚焦超声治疗用微粒类助剂及其应用
CN100427142C (zh) * 2005-01-10 2008-10-22 重庆海扶(Hifu)技术有限公司 一种高强度聚焦超声治疗用助剂及其筛选方法
CN100574809C (zh) * 2005-01-10 2009-12-30 重庆海扶(Hifu)技术有限公司 一种高强度聚焦超声治疗用氟碳乳剂类助剂及其应用
US10687785B2 (en) 2005-05-12 2020-06-23 The Trustees Of Columbia Univeristy In The City Of New York System and method for electromechanical activation of arrhythmias
WO2007035721A2 (en) * 2005-09-19 2007-03-29 The Trustees Of Columbia University In The City Of New York Ultrasound method to open blood brain barrier
KR101658439B1 (ko) 2005-12-09 2016-09-21 브라코 스위스 에스.에이. 표적화 벡터-포스포리피드 컨쥬게이트
EP1797919A1 (en) 2005-12-16 2007-06-20 Bracco Research S.A. Liquid transfer device for medical dispensing containers
US20070179094A1 (en) 2006-01-31 2007-08-02 Bayer Schering Pharma Ag Modulation of MDL-1 activity for treatment of inflammatory disease
US7967753B2 (en) 2006-08-01 2011-06-28 Stichting Voor de Technische Wetenschappen of Van Vollenhovenlaan Pulse inversion sequences for nonlinear imaging
WO2008027520A2 (en) * 2006-08-30 2008-03-06 The Trustees Of Columbia University In The City Of New York Systems and methods for composite elastography and wave imaging
WO2008028917A1 (en) 2006-09-05 2008-03-13 Bracco Research Sa Gas-filled microvesicles with polymer-modified lipids
EP2005970A1 (de) 2007-06-22 2008-12-24 Berlin Science Partners GmbH i.V. Bildgebende Diagnostik durch Kombination von Kontrastmitteln
JP2010005512A (ja) * 2008-06-25 2010-01-14 Kao Corp 微細気泡前駆体組成物
GB0811856D0 (en) * 2008-06-27 2008-07-30 Ucl Business Plc Magnetic microbubbles, methods of preparing them and their uses
WO2010014977A1 (en) 2008-08-01 2010-02-04 The Trustees Of Columbia University In The City Of New York Systems and methods for matching and imaging tissue characteristics
WO2010030779A1 (en) * 2008-09-10 2010-03-18 The Trustees Of Columbia University In The City Of New York Isolation of microbubbles of selected size range from polydisperse microbubbles
WO2010030819A1 (en) 2008-09-10 2010-03-18 The Trustees Of Columbia University In The City Of New York Systems and methods for opening a tissue
WO2011025893A1 (en) 2009-08-28 2011-03-03 The Trustees Of Columbia University In The City Of New York Systems, methods, and devices for production of gas-filled microbubbles
WO2011034892A2 (en) * 2009-09-15 2011-03-24 The Trustees Of Columbia University In The City Of New York Systems, methods, and devices for microbubbles
EP2480144B1 (en) 2009-09-21 2024-03-06 The Trustees of Columbia University in the City of New York Systems for opening of a tissue barrier
US9700640B2 (en) * 2009-09-21 2017-07-11 Drexel University Stabilized ultrasound contrast agent
US8834846B2 (en) 2010-05-06 2014-09-16 Bruker Biospin Corporation Fluorescent NIRF activatable probes for disease detection
WO2012019172A1 (en) * 2010-08-06 2012-02-09 The Trustees Of Columbia University In The City Of New York Medical imaging contrast devices, methods, and systems
WO2012136813A2 (en) 2011-04-07 2012-10-11 Universitetet I Oslo Agents for medical radar diagnosis
WO2012162664A1 (en) 2011-05-26 2012-11-29 The Trustees Of Columbia University In The City Of New York Systems and methods for opening of a tissue barrier in primates
BR112014026783B1 (pt) 2012-04-30 2020-12-15 Ge Healthcare As Processo para preparar um recipiente enchido com uma composição
EP2864033B1 (en) 2012-06-26 2016-04-06 GE Healthcare AS Preparation of composition comprising gas microbubbles
WO2014059170A1 (en) 2012-10-10 2014-04-17 The Trustees Of Columbia University In The City Of New York Systems and methods for mechanical mapping of cardiac rhythm
WO2014096165A2 (en) 2012-12-21 2014-06-26 Bracco Suisse Sa Gas-filled microvesicles
US9255907B2 (en) 2013-03-14 2016-02-09 Empire Technology Development Llc Identification of surgical smoke
EP2983687B1 (en) 2013-03-15 2019-09-04 The Regents of The University of California Peptides having reduced toxicity that stimulate cholesterol efflux
US9247921B2 (en) 2013-06-07 2016-02-02 The Trustees Of Columbia University In The City Of New York Systems and methods of high frame rate streaming for treatment monitoring
US10322178B2 (en) 2013-08-09 2019-06-18 The Trustees Of Columbia University In The City Of New York Systems and methods for targeted drug delivery
US10028723B2 (en) 2013-09-03 2018-07-24 The Trustees Of Columbia University In The City Of New York Systems and methods for real-time, transcranial monitoring of blood-brain barrier opening
GB201405735D0 (en) * 2014-03-31 2014-05-14 Ge Healthcare As Ultrasound precursor preparation method
SI3233136T1 (sl) 2014-12-18 2019-08-30 Bracco Suisse Sa Formulacije s plinom napolnjenih usmerjenih mikroveziklov
EP3308779B1 (en) * 2015-06-10 2019-08-07 Teikyo University Theranostic bubble preparation (tb), and method for using same
CN107233583B (zh) * 2016-03-29 2020-04-24 北京飞锐达医疗科技有限公司 一种具有超长持续时间的超声造影剂及其制备方法
GB201821049D0 (en) * 2018-12-21 2019-02-06 Ge Healthcare As Ultrasound contrast agent and methods for use therof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585112A (en) * 1989-12-22 1996-12-17 Imarx Pharmaceutical Corp. Method of preparing gas and gaseous precursor-filled microspheres
US5445813A (en) * 1992-11-02 1995-08-29 Bracco International B.V. Stable microbubble suspensions as enhancement agents for ultrasound echography
IN172208B (Direct) * 1990-04-02 1993-05-01 Sint Sa
DE4100470A1 (de) * 1991-01-09 1992-07-16 Byk Gulden Lomberg Chem Fab Echokontrastmittel
GB9106673D0 (en) * 1991-03-28 1991-05-15 Hafslund Nycomed As Improvements in or relating to contrast agents
DE4328642A1 (de) * 1993-08-26 1995-03-02 Byk Gulden Lomberg Chem Fab Ultraschallkontrastmittel
GB9511488D0 (en) * 1995-06-07 1995-08-02 Nycomed Imaging As Improvements in or relating to contrast agents
US5846517A (en) * 1996-09-11 1998-12-08 Imarx Pharmaceutical Corp. Methods for diagnostic imaging using a renal contrast agent and a vasodilator

Also Published As

Publication number Publication date
AU1884797A (en) 1997-09-02
AP890A (en) 2000-11-13
EP0881915A1 (en) 1998-12-09
ATE238072T1 (de) 2003-05-15
US20010010811A1 (en) 2001-08-02
HUP9900813A3 (en) 1999-11-29
OA10839A (en) 2001-08-13
PT881915E (pt) 2003-09-30
IL125748A0 (en) 1999-04-11
CN1278740C (zh) 2006-10-11
EP1331013A3 (en) 2004-03-24
JP2000511510A (ja) 2000-09-05
JP4418033B2 (ja) 2010-02-17
SK112698A3 (en) 1999-04-13
HUP9900813A2 (hu) 1999-07-28
CN1213972A (zh) 1999-04-14
SK284200B6 (sk) 2004-10-05
CA2246779A1 (en) 1997-08-21
WO1997029783A1 (en) 1997-08-21
AP9801319A0 (en) 1998-09-30
EA001135B1 (ru) 2000-10-30
US6221337B1 (en) 2001-04-24
EE9800249A (et) 1999-02-15
DE69721235T2 (de) 2004-02-05
US20030202942A1 (en) 2003-10-30
IL125748A (en) 2002-05-23
KR100500334B1 (ko) 2005-09-09
CA2246779C (en) 2008-04-29
HU229090B1 (en) 2013-07-29
AU726503B2 (en) 2000-11-09
HK1014872A1 (en) 1999-10-08
NZ331509A (en) 2000-04-28
KR19990082669A (ko) 1999-11-25
PL328406A1 (en) 1999-01-18
CZ262598A3 (cs) 1999-05-12
BG102758A (en) 1999-09-30
CZ293986B6 (cs) 2004-09-15
ES2197986T3 (es) 2004-01-16
EP0881915B1 (en) 2003-04-23
TR199801613T2 (xx) 1998-11-23
EP1331013A2 (en) 2003-07-30
DE69721235D1 (de) 2003-05-28
US20050201942A1 (en) 2005-09-15
EA199800745A1 (ru) 1999-02-25

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