EA201591283A1 - Количественная оценка эффективности кэп матричной рнк - Google Patents

Количественная оценка эффективности кэп матричной рнк

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Publication number
EA201591283A1
EA201591283A1 EA201591283A EA201591283A EA201591283A1 EA 201591283 A1 EA201591283 A1 EA 201591283A1 EA 201591283 A EA201591283 A EA 201591283A EA 201591283 A EA201591283 A EA 201591283A EA 201591283 A1 EA201591283 A1 EA 201591283A1
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EA
Eurasian Patent Office
Prior art keywords
efficiency
cep
quantitative estimation
matrix rna
mrna
Prior art date
Application number
EA201591283A
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English (en)
Inventor
Майкл Хартлейн
Франк Дероза
Ануша Диаз
Original Assignee
Шир Хьюман Дженетик Терапис, Инк.
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Application filed by Шир Хьюман Дженетик Терапис, Инк. filed Critical Шир Хьюман Дженетик Терапис, Инк.
Publication of EA201591283A1 publication Critical patent/EA201591283A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase

Abstract

Настоящее изобретение предоставляет, помимо прочего, способы количественной оценки эффективности кэпирования мРНК, в особенности мРНК, синтезированной in vitro. В некоторых вариантах осуществления способы включают хроматографические способы количественного определения эффективности кэпирования мРНК и статуса метилирования кэпов.
EA201591283A 2013-03-14 2014-03-14 Количественная оценка эффективности кэп матричной рнк EA201591283A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361784337P 2013-03-14 2013-03-14
PCT/US2014/027587 WO2014152659A1 (en) 2013-03-14 2014-03-14 Quantitative assessment for cap efficiency of messenger rna

Publications (1)

Publication Number Publication Date
EA201591283A1 true EA201591283A1 (ru) 2016-02-29

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EA201591283A EA201591283A1 (ru) 2013-03-14 2014-03-14 Количественная оценка эффективности кэп матричной рнк

Country Status (13)

Country Link
US (3) US9970047B2 (ru)
EP (1) EP2971102B8 (ru)
JP (1) JP2016514970A (ru)
CN (1) CN105051213A (ru)
AU (1) AU2014239250A1 (ru)
BR (1) BR112015022505A2 (ru)
CA (1) CA2903487A1 (ru)
DK (1) DK2971102T3 (ru)
EA (1) EA201591283A1 (ru)
ES (1) ES2680595T3 (ru)
MX (1) MX2015011944A (ru)
TR (1) TR201811157T4 (ru)
WO (1) WO2014152659A1 (ru)

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JP2016514970A (ja) 2016-05-26
ES2680595T3 (es) 2018-09-10
BR112015022505A2 (pt) 2017-10-24
CN105051213A (zh) 2015-11-11
US20160032356A1 (en) 2016-02-04
WO2014152659A1 (en) 2014-09-25
DK2971102T3 (en) 2018-08-27
EP2971102A1 (en) 2016-01-20
AU2014239250A1 (en) 2015-08-27
EP2971102B8 (en) 2018-08-15
US20180291425A1 (en) 2018-10-11
CA2903487A1 (en) 2014-09-25
TR201811157T4 (tr) 2018-08-27
US20220364145A1 (en) 2022-11-17
US9970047B2 (en) 2018-05-15
US11365437B2 (en) 2022-06-21

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