BR112015022505A2 - avaliação quantitativa para eficiência do cap de rna mensageiro - Google Patents

avaliação quantitativa para eficiência do cap de rna mensageiro

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Publication number
BR112015022505A2
BR112015022505A2 BR112015022505A BR112015022505A BR112015022505A2 BR 112015022505 A2 BR112015022505 A2 BR 112015022505A2 BR 112015022505 A BR112015022505 A BR 112015022505A BR 112015022505 A BR112015022505 A BR 112015022505A BR 112015022505 A2 BR112015022505 A2 BR 112015022505A2
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Brazil
Prior art keywords
quantitative evaluation
efficiency
messenger rna
rna cap
cap efficiency
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Application number
BR112015022505A
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English (en)
Inventor
Dias Anusha
Derosa Frank
Heartlein Michael
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Shire Human Genetic Therapies
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Publication date
Application filed by Shire Human Genetic Therapies filed Critical Shire Human Genetic Therapies
Publication of BR112015022505A2 publication Critical patent/BR112015022505A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

resumo avaliação quantitativa para eficiência do cap de rna mensageiro a presente invenção proporciona, entre outras coisas, métodos de quantificação de eficiência de capeamento de mrna, particularmente para mrna sintetizado in vitro. em algumas modalidades, os métodos compreendem métodos cromatográficos de quantificar a eficiência de capeamento e estado de metilação dos capeamentos.
BR112015022505A 2013-03-14 2014-03-14 avaliação quantitativa para eficiência do cap de rna mensageiro BR112015022505A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361784337P 2013-03-14 2013-03-14
PCT/US2014/027587 WO2014152659A1 (en) 2013-03-14 2014-03-14 Quantitative assessment for cap efficiency of messenger rna

Publications (1)

Publication Number Publication Date
BR112015022505A2 true BR112015022505A2 (pt) 2017-10-24

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BR112015022505A BR112015022505A2 (pt) 2013-03-14 2014-03-14 avaliação quantitativa para eficiência do cap de rna mensageiro

Country Status (13)

Country Link
US (3) US9970047B2 (pt)
EP (1) EP2971102B8 (pt)
JP (1) JP2016514970A (pt)
CN (1) CN105051213A (pt)
AU (1) AU2014239250A1 (pt)
BR (1) BR112015022505A2 (pt)
CA (1) CA2903487A1 (pt)
DK (1) DK2971102T3 (pt)
EA (1) EA201591283A1 (pt)
ES (1) ES2680595T3 (pt)
MX (1) MX2015011944A (pt)
TR (1) TR201811157T4 (pt)
WO (1) WO2014152659A1 (pt)

Families Citing this family (128)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL3338765T3 (pl) 2009-12-01 2019-06-28 Translate Bio, Inc. Pochodna steroidowa dla dostarczania mrna w ludzkich chorobach genetycznych
DE12722942T1 (de) 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
CN111671918A (zh) 2011-06-08 2020-09-18 川斯勒佰尔公司 Mrna递送的脂质纳米颗粒组合物和方法
EP3336082B1 (en) 2011-06-08 2020-04-15 Translate Bio, Inc. Cleavable lipids
US20150267192A1 (en) 2012-06-08 2015-09-24 Shire Human Genetic Therapies, Inc. Nuclease resistant polynucleotides and uses thereof
ES2708561T3 (es) 2013-03-14 2019-04-10 Translate Bio Inc Métodos para la purificación de ARN mensajero
JP2016514970A (ja) 2013-03-14 2016-05-26 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド メッセンジャーｒｎａのキャップ効率の定量的評価
EP2971098B1 (en) 2013-03-14 2018-11-21 Translate Bio, Inc. Quantitative assessment for cap efficiency of messenger rna
AU2014236305B2 (en) 2013-03-14 2019-01-17 Ethris Gmbh CFTR mRNA compositions and related methods and uses
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
EP2983804A4 (en) 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ion exchange purification of mrna
EP2971033B8 (en) 2013-03-15 2019-07-10 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
EP4279610A3 (en) 2013-03-15 2024-01-03 ModernaTX, Inc. Ribonucleic acid purification
EP3052511A4 (en) 2013-10-02 2017-05-31 Moderna Therapeutics, Inc. Polynucleotide molecules and uses thereof
JP6506749B2 (ja) 2013-10-22 2019-04-24 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド フェニルケトン尿症のためのｍＲＮＡ療法
CN106413811A (zh) 2013-10-22 2017-02-15 夏尔人类遗传性治疗公司 精氨基琥珀酸合成酶缺乏症的mrna疗法
WO2015101416A1 (en) * 2013-12-30 2015-07-09 Curevac Gmbh Methods for rna analysis
EP3540060A1 (en) * 2013-12-30 2019-09-18 CureVac AG Methods for rna analysis
ES2750661T3 (es) 2014-04-25 2020-03-26 Translate Bio Inc Métodos para la purificación de ARN mensajero
WO2015196128A2 (en) 2014-06-19 2015-12-23 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
US10407683B2 (en) 2014-07-16 2019-09-10 Modernatx, Inc. Circular polynucleotides
EP3317424B1 (en) * 2015-07-01 2023-09-06 CureVac Manufacturing GmbH Method for analysis of an rna molecule
AU2016324463B2 (en) 2015-09-17 2022-10-27 Modernatx, Inc. Polynucleotides containing a stabilizing tail region
US11434486B2 (en) 2015-09-17 2022-09-06 Modernatx, Inc. Polynucleotides containing a morpholino linker
US10144942B2 (en) 2015-10-14 2018-12-04 Translate Bio, Inc. Modification of RNA-related enzymes for enhanced production
US20210108252A1 (en) 2015-12-09 2021-04-15 Novartis Ag Label-free analysis of rna capping efficiency using rnase h, probes and liquid chromatography/mass spectrometry
US20210206818A1 (en) 2016-01-22 2021-07-08 Modernatx, Inc. Messenger ribonucleic acids for the production of intracellular binding polypeptides and methods of use thereof
WO2017149139A1 (en) * 2016-03-03 2017-09-08 Curevac Ag Rna analysis by total hydrolysis
EP3442590A2 (en) 2016-04-13 2019-02-20 Modernatx, Inc. Lipid compositions and their uses for intratumoral polynucleotide delivery
HRP20230050T1 (hr) 2016-05-18 2023-03-03 Modernatx, Inc. Polinukleotidi koji kodiraju interleukin-12 (il12) i njihova upotreba
MA46756A (fr) 2016-11-10 2019-09-18 Translate Bio Inc Formulation de nanoparticules lipidiques à base de glace améliorée pour l'administration de l'arnm
IL268857B2 (en) 2017-02-27 2024-09-01 Translate Bio Inc Methods for the purification of messenger RNA
WO2018157154A2 (en) 2017-02-27 2018-08-30 Translate Bio, Inc. Novel codon-optimized cftr mrna
DK3585892T3 (da) 2017-02-27 2022-08-22 Translate Bio Inc Fremgangsmåder til oprensning af messenger-rna
US11560562B2 (en) 2017-02-27 2023-01-24 Translate Bio, Inc. Large-scale synthesis of messenger RNA
EP3624824B1 (en) 2017-05-16 2024-07-10 Translate Bio, Inc. Codon-optimized mrna encoding cftr for use in treating cystic fibrosis
WO2018231990A2 (en) 2017-06-14 2018-12-20 Modernatx, Inc. Polynucleotides encoding methylmalonyl-coa mutase
CN118421617A (zh) 2018-08-24 2024-08-02 川斯勒佰尔公司 用于纯化信使rna的方法
EP3877538A1 (en) 2018-11-08 2021-09-15 Translate Bio, Inc. Methods and compositions for messenger rna purification
EP3883592A1 (en) 2018-11-21 2021-09-29 Translate Bio, Inc. Treatment of cystic fibrosis by delivery of nebulized mrna encoding cftr
US20220040281A1 (en) 2018-12-21 2022-02-10 Curevac Ag Rna for malaria vaccines
CA3122645A1 (en) 2018-12-21 2020-06-25 Curevac Ag Methods for rna analysis
WO2020168466A1 (en) 2019-02-19 2020-08-27 Stemirna Therapeutics Co., Ltd. Modified nucleoside and synthetic methods thereof
MX2021011031A (es) * 2019-03-11 2021-12-10 Modernatx Inc Proceso de transcripción in vitro en lotes alimentados.
US20220177423A1 (en) 2019-04-18 2022-06-09 Translate Bio, Inc. Cystine cationic lipids
ES2972014T3 (es) 2019-04-22 2024-06-10 Translate Bio Inc Lípidos catiónicos de tioéster
WO2020227085A1 (en) 2019-05-03 2020-11-12 Translate Bio, Inc. Di-thioester cationic lipids
KR20220029556A (ko) 2019-05-15 2022-03-08 트랜슬레이트 바이오 인코포레이티드 전령 rna의 정제 방법
EP3986452A1 (en) 2019-06-18 2022-04-27 CureVac AG Rotavirus mrna vaccine
CN114401942B (zh) 2019-06-21 2024-01-26 川斯勒佰尔公司 三(羟甲基)甲基甘氨酸和柠檬酸脂质
WO2020257611A1 (en) 2019-06-21 2020-12-24 Translate Bio, Inc. Cationic lipids comprising an hydroxy moiety
WO2021021988A1 (en) 2019-07-30 2021-02-04 Translate Bio, Inc. Treatment of cystic fibrosis by delivery of nebulized mrna encoding cftr
JP2022544412A (ja) 2019-08-14 2022-10-18 キュアバック アーゲー 免疫賦活特性が減少したｒｎａ組み合わせおよび組成物
EP4022068A1 (en) * 2019-08-29 2022-07-06 Universität Zürich Minimal messenger rnas and uses thereof
EP4031662A1 (en) 2019-09-20 2022-07-27 Translate Bio, Inc. Mrna encoding engineered cftr
EP4041400A1 (en) 2019-10-09 2022-08-17 Translate Bio, Inc. Compositions, methods and uses of messenger rna
US20210145860A1 (en) 2019-10-21 2021-05-20 Translate Bio, Inc. Compositions, methods and uses of messenger rna
CA3165388A1 (en) 2019-12-20 2021-06-24 Translate Bio, Inc. Improved process of preparing mrna-loaded lipid nanoparticles
KR20220142432A (ko) 2019-12-20 2022-10-21 트랜슬레이트 바이오 인코포레이티드 메신저 rna의 직장 전달
AU2021216658A1 (en) 2020-02-04 2022-06-23 CureVac SE Coronavirus vaccine
US20210324369A1 (en) 2020-02-10 2021-10-21 Translate Bio, Inc. Methods and compositions for messenger rna purification
CN115398546A (zh) 2020-02-18 2022-11-25 翻译生物公司 用于信使rna体外转录的改进方法
WO2021173840A1 (en) 2020-02-25 2021-09-02 Translate Bio, Inc. Improved processes of preparing mrna-loaded lipid nanoparticles
GB202307565D0 (en) 2020-04-22 2023-07-05 BioNTech SE Coronavirus vaccine
CA3177940A1 (en) 2020-05-07 2021-11-11 Anusha DIAS Optimized nucleotide sequences encoding sars-cov-2 antigens
AU2021268028A1 (en) 2020-05-07 2023-01-19 Translate Bio, Inc. Generation of optimized nucleotide sequences
EP4146342A1 (en) 2020-05-07 2023-03-15 Translate Bio, Inc. Improved compositions for cftr mrna therapy
WO2021226463A1 (en) 2020-05-07 2021-11-11 Translate Bio, Inc. Composition and methods for treatment of primary ciliary dyskinesia
AU2021273502A1 (en) 2020-05-15 2023-02-02 Translate Bio, Inc. Lipid nanoparticle formulations for mRNA delivery
CA3170740A1 (en) 2020-05-29 2021-12-02 Curevac Ag Nucleic acid based combination vaccines
WO2022023559A1 (en) 2020-07-31 2022-02-03 Curevac Ag Nucleic acid encoded antibody mixtures
CN112111558A (zh) * 2020-08-19 2020-12-22 翌圣生物科技（上海）有限公司 一种测定牛痘病毒加帽酶活性的方法
EP4157344A2 (en) 2020-08-31 2023-04-05 CureVac SE Multivalent nucleic acid based coronavirus vaccines
CN117255711A (zh) 2020-10-01 2023-12-19 翻译生物公司 用于纯化信使rna的方法
JP2023544197A (ja) 2020-10-06 2023-10-20 トランスレイト バイオ， インコーポレイテッド 脂質ナノ粒子の改善された方法および製剤
WO2022081548A1 (en) 2020-10-12 2022-04-21 Translate Bio, Inc. Improved process of preparing ice-based lipid nanoparticles
AU2021361986A1 (en) 2020-10-12 2023-06-15 Translate Bio, Inc. Improved process of preparing mrna-loaded lipid nanoparticles
CN116438298A (zh) 2020-10-15 2023-07-14 翻译生物公司 信使rna的大规模合成
US11771652B2 (en) 2020-11-06 2023-10-03 Sanofi Lipid nanoparticles for delivering mRNA vaccines
WO2022099194A1 (en) 2020-11-09 2022-05-12 Translate Bio, Inc. Improved compositions for delivery of codon-optimized mrna
CA3199895A1 (en) 2020-11-25 2022-06-02 Shrirang KARVE Stable liquid lipid nanoparticle formulations
CA3171051A1 (en) 2020-12-22 2022-06-30 Curevac Ag Pharmaceutical composition comprising lipid-based carriers encapsulating rna for multidose administration
WO2022137133A1 (en) 2020-12-22 2022-06-30 Curevac Ag Rna vaccine against sars-cov-2 variants
EP4277929A1 (en) 2021-01-14 2023-11-22 Translate Bio, Inc. Methods and compositions for delivering mrna coded antibodies
US20240102065A1 (en) 2021-01-27 2024-03-28 CureVac SE Method of reducing the immunostimulatory properties of in vitro transcribed rna
EP4313115A1 (en) 2021-03-25 2024-02-07 Translate Bio, Inc. Optimized nucleotide sequences encoding the extracellular domain of human ace2 protein or a portion thereof
WO2022200575A1 (en) 2021-03-26 2022-09-29 Glaxosmithkline Biologicals Sa Immunogenic compositions
EP4330427A1 (en) 2021-04-29 2024-03-06 Translate Bio, Inc. Methods for measuring poly a tail length
WO2022233880A1 (en) 2021-05-03 2022-11-10 Curevac Ag Improved nucleic acid sequence for cell type specific expression
EP4347875A1 (en) * 2021-06-04 2024-04-10 Translate Bio, Inc. Assay for quantitative assessment of mrna capping efficiency
MX2023015464A (es) 2021-06-18 2024-01-18 Sanofi Sa Vacunas multivalentes contra la influenza.
WO2023023487A1 (en) 2021-08-16 2023-02-23 Translate Bio, Inc. Screening codon-optimized nucleotide sequences
WO2023031392A2 (en) 2021-09-03 2023-03-09 CureVac SE Novel lipid nanoparticles for delivery of nucleic acids comprising phosphatidylserine
AU2022382104A1 (en) 2021-11-05 2024-06-20 Sanofi Respiratory syncytial virus rna vaccine
EP4429713A1 (en) 2021-11-10 2024-09-18 Translate Bio, Inc. Composition and methods for treatment of primary ciliary dyskinesia
IL312997A (en) 2021-11-30 2024-07-01 Sanofi Pasteur Inc Human metapneumovirus vaccines
EP4448103A1 (en) 2021-12-17 2024-10-23 Sanofi Lyme disease rna vaccine
WO2023115495A1 (zh) * 2021-12-23 2023-06-29 北京毅新博创生物科技有限公司 质谱法检测mRNA加帽效率的方法
CN114250268A (zh) * 2021-12-23 2022-03-29 北京毅新博创生物科技有限公司 检测mRNA样品的加帽效率的产品及用途
CN114894916A (zh) * 2022-04-02 2022-08-12 翌圣生物科技(上海)股份有限公司 一种检测rna加帽效率的方法
JP2024522732A (ja) * 2022-04-15 2024-06-21 サーモ フィニガン リミテッド ライアビリティ カンパニー Ｍｒｎａキャッピング効率の定量的モニタリング方法
WO2023214082A2 (en) 2022-05-06 2023-11-09 Sanofi Signal sequences for nucleic acid vaccines
CN114574466B (zh) * 2022-05-09 2022-08-02 翌圣生物科技(上海)股份有限公司 一种嵌合加帽酶及其制备方法与应用
JP2023181989A (ja) 2022-06-13 2023-12-25 上海臻上医薬科技有限公司 マイクロニードル注射製剤およびその使用
US11878055B1 (en) 2022-06-26 2024-01-23 BioNTech SE Coronavirus vaccine
GB202209732D0 (en) * 2022-07-01 2022-08-17 Sintef Tto As Method of analysing rna
TW202424185A (zh) 2022-07-29 2024-06-16 法商賽諾菲公司 無乙醇之mRNA純化方法
WO2024044108A1 (en) 2022-08-22 2024-02-29 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Vaccines against coronaviruses
TW202417510A (zh) 2022-09-20 2024-05-01 美商威特拉公司 用ｃ３ｂ抗體治療補體介導之疾病及病症
WO2024068545A1 (en) 2022-09-26 2024-04-04 Glaxosmithkline Biologicals Sa Influenza virus vaccines
WO2024083345A1 (en) 2022-10-21 2024-04-25 BioNTech SE Methods and uses associated with liquid compositions
WO2024089638A1 (en) 2022-10-28 2024-05-02 Glaxosmithkline Biologicals Sa Nucleic acid based vaccine
WO2024094881A1 (en) 2022-11-04 2024-05-10 Sanofi Respiratory syncytial virus rna vaccination
WO2024094876A1 (en) 2022-11-04 2024-05-10 Sanofi Methods for messenger rna tailing
WO2024104914A1 (en) 2022-11-14 2024-05-23 BioNTech SE Rna capping efficiency assay
WO2024112652A1 (en) 2022-11-21 2024-05-30 Translate Bio, Inc. Compositions of dry powder formulations of messenger rna and methods of use thereof
WO2024126809A1 (en) 2022-12-15 2024-06-20 Sanofi Mrna encoding influenza virus-like particle
WO2024126847A1 (en) 2022-12-15 2024-06-20 Sanofi Mrna recombinant capping enzymes
WO2024133515A1 (en) 2022-12-20 2024-06-27 Sanofi Rhinovirus mrna vaccine
WO2024133884A2 (en) 2022-12-23 2024-06-27 Sanofi Optimized tailing of messenger rna
WO2024160936A1 (en) 2023-02-03 2024-08-08 Glaxosmithkline Biologicals Sa Rna formulation
GB202302092D0 (en) 2023-02-14 2023-03-29 Glaxosmithkline Biologicals Sa Analytical method
WO2024170684A1 (en) 2023-02-15 2024-08-22 Sanofi Screening codon-optimized nucleotide sequences
US20240299524A1 (en) 2023-03-02 2024-09-12 Sanofi Compositions for use in treatment of chlamydia
WO2024184489A1 (en) 2023-03-07 2024-09-12 Sanofi Manufacture of messenger rna with kp34 polymerase
CN116426610B (zh) * 2023-06-08 2024-01-05 启辰生生物科技(珠海)有限公司 一种LC-MS法准确定量mRNA加帽效率的方法
CN116590382B (zh) * 2023-07-12 2023-11-10 上海奥浦迈生物科技股份有限公司 检测mRNA样品加帽率的探针、试剂盒及其使用方法
GB202404607D0 (en) 2024-03-29 2024-05-15 Glaxosmithkline Biologicals Sa RNA formulation

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
EP0463109A4 (en) 1989-03-24 1992-11-19 Research Corporation Technologies, Inc. Recombinant alpha-galactosidase, a therapy for fabry disease
US5356804A (en) 1990-10-24 1994-10-18 Mount Sinai School Of Medicine Of The City Of New York Cloning and expression of biologically active human α-galactosidase A
DE4140463A1 (de) 1991-12-09 1993-06-17 Boehringer Mannheim Gmbh 2'-desoxy-isoguanosin, dessen isostere analoge sowie anwendung derselben
US5256555A (en) 1991-12-20 1993-10-26 Ambion, Inc. Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions
US5268289A (en) 1991-12-27 1993-12-07 Epicentre Technologies Corp. Thermostable ribonuclease H
US5459055A (en) 1991-12-27 1995-10-17 Epicentre Technologies Corporation Thermostable ribonuclease H isolated from Thermus flavus
CN1231695A (zh) 1996-08-16 1999-10-13 同和药品工业株式会社 HBV聚合酶,起源于HBV聚合酶的RNaseH,其制备过程和在筛选抗病毒剂中的应用
US6458574B1 (en) 1996-09-12 2002-10-01 Transkaryotic Therapies, Inc. Treatment of a α-galactosidase a deficiency
ATE232880T1 (de) 1996-11-18 2003-03-15 Takeshi Imanishi Neue nucleotidanaloga
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
US6143877A (en) 1997-04-30 2000-11-07 Epoch Pharmaceuticals, Inc. Oligonucleotides including pyrazolo[3,4-D]pyrimidine bases, bound in double stranded nucleic acids
EP2341058A3 (en) 1997-09-12 2011-11-23 Exiqon A/S Oligonucleotide Analogues
WO1999028447A1 (en) 1997-12-04 1999-06-10 Isis Pharmaceuticals, Inc. Human rnase h and compositions and uses thereof
US6127121A (en) 1998-04-03 2000-10-03 Epoch Pharmaceuticals, Inc. Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination
US6001652A (en) 1998-09-18 1999-12-14 Isis Pharmaceuticals Inc. Antisense modulation of cREL expression
CA2384407C (en) 1999-08-30 2009-10-20 Roche Diagnostics Gmbh 2-azapurine compounds and their use
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US20020197622A1 (en) * 2001-01-31 2002-12-26 Mcdevitt John T. Method and apparatus for the confinement of materials in a micromachined chemical sensor array
WO2004020575A2 (en) 2002-06-24 2004-03-11 Exiqon A/S Methods and systems for detection and isolation of a nucleotide sequence
EP2049665A2 (en) 2006-07-28 2009-04-22 Applera Corporation Dinucleotide mrna cap analogs
DE102006061015A1 (de) 2006-12-22 2008-06-26 Curevac Gmbh Verfahren zur Reinigung von RNA im präparativen Maßstab mittels HPLC
WO2009058911A2 (en) 2007-10-31 2009-05-07 Applied Biosystems Inc. Preparation and isolation of 5' capped mrna
JP2011521626A (ja) * 2008-05-02 2011-07-28 エピセンター テクノロジーズ コーポレーション Ｒｎａポリホスファターゼの組成物、キットおよびそれらの使用
US20100035249A1 (en) 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
DE12722942T1 (de) * 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
EP2971098B1 (en) 2013-03-14 2018-11-21 Translate Bio, Inc. Quantitative assessment for cap efficiency of messenger rna
JP2016514970A (ja) 2013-03-14 2016-05-26 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド メッセンジャーｒｎａのキャップ効率の定量的評価
WO2017149139A1 (en) 2016-03-03 2017-09-08 Curevac Ag Rna analysis by total hydrolysis
MX2020011167A (es) 2018-04-25 2021-02-09 Ethris Gmbh Formulaciones a base de lipidos para el suministro de arn.

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JP2016514970A (ja) 2016-05-26
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CN105051213A (zh) 2015-11-11
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EA201591283A1 (ru) 2016-02-29
US9970047B2 (en) 2018-05-15
US12084711B2 (en) 2024-09-10
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US20160032356A1 (en) 2016-02-04
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