BR112016005957A2 - detecção de alvo multiaptâmero - Google Patents

detecção de alvo multiaptâmero

Info

Publication number
BR112016005957A2
BR112016005957A2 BR112016005957A BR112016005957A BR112016005957A2 BR 112016005957 A2 BR112016005957 A2 BR 112016005957A2 BR 112016005957 A BR112016005957 A BR 112016005957A BR 112016005957 A BR112016005957 A BR 112016005957A BR 112016005957 A2 BR112016005957 A2 BR 112016005957A2
Authority
BR
Brazil
Prior art keywords
multiaptamer
target detection
aptamer
compositions
target
Prior art date
Application number
BR112016005957A
Other languages
English (en)
Inventor
Gold Larry
S Green Louis
Janjic Nebojsa
A Ochsner Urs
Original Assignee
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Somalogic Inc filed Critical Somalogic Inc
Publication of BR112016005957A2 publication Critical patent/BR112016005957A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)

Abstract

a presente invenção refere-se a composições compreendendo um primeiro aptâmero, segundo aptâmero e um alvo que são capazes de formar um complexo ternário, e em que o primeiro aptâmero e o segundo aptâmero compreendem esquemas de modificação de pirimidina c-5 que são diferentes e métodos para preparar e usar essas composições.
BR112016005957A 2013-09-24 2014-09-24 detecção de alvo multiaptâmero BR112016005957A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361881629P 2013-09-24 2013-09-24
PCT/US2014/057143 WO2015048084A1 (en) 2013-09-24 2014-09-24 Multiaptamer target detection

Publications (1)

Publication Number Publication Date
BR112016005957A2 true BR112016005957A2 (pt) 2017-09-26

Family

ID=52744404

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016005957A BR112016005957A2 (pt) 2013-09-24 2014-09-24 detecção de alvo multiaptâmero

Country Status (13)

Country Link
US (2) US9926566B2 (pt)
EP (1) EP3049523B1 (pt)
JP (1) JP6506768B2 (pt)
KR (1) KR102348283B1 (pt)
CN (2) CN114703193A (pt)
AU (1) AU2014326975B2 (pt)
BR (1) BR112016005957A2 (pt)
CA (1) CA2924987C (pt)
HK (1) HK1218765A1 (pt)
IL (1) IL244284B (pt)
MX (1) MX361451B (pt)
SG (1) SG11201602063UA (pt)
WO (1) WO2015048084A1 (pt)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201602063UA (en) 2013-09-24 2016-04-28 Somalogic Inc Multiaptamer target detection
JP6768651B2 (ja) 2014-11-24 2020-10-14 ソマロジック, インコーポレイテッドSomaLogic, Inc. 増殖分化因子１１と結合するための核酸化合物
US11332784B2 (en) * 2015-12-08 2022-05-17 Twinstrand Biosciences, Inc. Adapters, methods, and compositions for duplex sequencing
SG10201911357VA (en) 2016-03-14 2020-01-30 Somalogic Inc Compounds and methods for the synthesis of 5-(n-protected-tryptaminocarboxyamide)-2'-deoxyuridine phosphoramidate for incorporation into a nucleic sequence
EP3472357A4 (en) * 2016-06-17 2020-01-22 Proximity Biosciences, LLC METHOD FOR SELECTING A PAIR OF APTAMERS
RU2021137915A (ru) * 2016-07-01 2022-02-04 Сомалоджик, Инк. Олигонуклеотиды, содержащие модифицированные нуклеозиды
JP2020514746A (ja) 2016-12-01 2020-05-21 ノーティラス バイオテクノロジー インコーポレイテッド タンパク質をアッセイする方法
WO2019103131A1 (ja) * 2017-11-24 2019-05-31 国立大学法人 東京大学 アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法
MX2020013814A (es) * 2018-06-22 2021-04-13 Somalogic Operating Co Inc Ensayo multiple proteomico mejorado.
EP4165549A1 (en) 2020-06-11 2023-04-19 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
AU2022209365A1 (en) 2021-01-21 2023-07-20 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
IL305336A (en) 2021-03-11 2023-10-01 Nautilus Subsidiary Inc Systems and methods for the preservation of biomolecules
US20230070896A1 (en) 2021-09-09 2023-03-09 Nautilus Biotechnology, Inc. Characterization and localization of protein modifications
WO2023049073A1 (en) 2021-09-22 2023-03-30 Nautilus Biotechnology, Inc. Methods and systems for determining polypeptide interactions
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
WO2023192917A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
US20230360732A1 (en) 2022-04-25 2023-11-09 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
WO2024107857A1 (en) 2022-11-15 2024-05-23 Nautilus Subsidiary, Inc. Standard polypeptides
WO2024124073A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
WO2024130000A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays

Family Cites Families (21)

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AU2001257091A1 (en) * 2000-04-18 2001-10-30 Gilead Sciences, Inc. Aptamer based two-site binding assay
EP1330544A4 (en) * 2000-09-26 2005-04-06 Univ Duke RNA APTAMERS AND METHOD OF IDENTIFYING SAID APTAMERS
US20070071744A1 (en) 2002-06-07 2007-03-29 Gotz Munch Agents which bind to epitopes of glycoprotein VI
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
WO2007056250A2 (en) 2005-11-04 2007-05-18 U.S. Genomics, Inc. Heterogeneous assay of analytes in solution using polymers
WO2008039642A2 (en) 2006-09-26 2008-04-03 Replidyne, Inc. Methods and compounds for treatment of clostridium based infection
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
EP2172566B2 (en) * 2007-07-17 2022-05-18 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20100239682A1 (en) 2007-09-17 2010-09-23 Antoine Andremont Colonic delivery of antimicrobial agents
US8569252B2 (en) 2009-04-15 2013-10-29 Postech Academy-Industry Foundation Nucleolin specific aptamer and use thereof
EP2534164A4 (en) 2010-02-11 2013-09-11 Intelligent Med Devices Inc OLIGONUCLEOTIDES RELATING TO DIFFICULT CLOSTRIDIUM GENES ENCODING TOXIN B. TOXIN A OR TOXIN BINARY
GB201002627D0 (en) 2010-02-16 2010-03-31 Loxbridge Res Llp Aptamer based analyte detection method
EP3345915B1 (en) * 2010-04-12 2021-11-24 Somalogic, Inc. 5-position modified pyrimidines and their use
US8785132B2 (en) 2010-04-23 2014-07-22 Postech Academy-Industry Foundation Aptamer sandwich assays
AU2012225273B2 (en) 2011-03-10 2016-10-13 Somalogic, Inc. Aptamers for Clostridium difficile diagnostics
US20150031585A1 (en) 2011-03-10 2015-01-29 Somalogic, Inc. Multiaptamer Target Detection
RU2564855C2 (ru) * 2011-04-26 2015-10-10 ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "НоваМедика" Способ получения пэгилированных олигонуклеотидов
RU2651708C2 (ru) * 2011-09-30 2018-04-23 Сомалоджик, Инк. Прогнозирование риска сердечно-сосудистого события и его применение
MX2018004950A (es) 2011-12-30 2022-01-24 Quest Diagnostics Invest Inc Aptámeros y métodos de diagnóstico para detectar el receptor de egf.
SG11201602063UA (en) 2013-09-24 2016-04-28 Somalogic Inc Multiaptamer target detection

Also Published As

Publication number Publication date
CA2924987A1 (en) 2015-04-02
HK1218765A1 (zh) 2017-03-10
KR20160058777A (ko) 2016-05-25
US10392621B2 (en) 2019-08-27
IL244284B (en) 2019-12-31
EP3049523A1 (en) 2016-08-03
KR102348283B1 (ko) 2022-01-10
MX361451B (es) 2018-12-06
EP3049523A4 (en) 2017-05-17
CN105555957A (zh) 2016-05-04
MX2016003320A (es) 2016-05-31
AU2014326975A2 (en) 2017-01-05
US20180187197A1 (en) 2018-07-05
JP2016531593A (ja) 2016-10-13
AU2014326975B2 (en) 2020-05-07
JP6506768B2 (ja) 2019-04-24
EP3049523B1 (en) 2019-08-07
CN114703193A (zh) 2022-07-05
SG11201602063UA (en) 2016-04-28
CA2924987C (en) 2022-08-16
US9926566B2 (en) 2018-03-27
AU2014326975A1 (en) 2016-04-07
IL244284A0 (en) 2016-04-21
US20160237435A1 (en) 2016-08-18
WO2015048084A1 (en) 2015-04-02

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B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2599 DE 27-10-2020 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.

B350 Update of information on the portal [chapter 15.35 patent gazette]