MX361451B - Detección objetivo de múltiples aptámeros. - Google Patents

Detección objetivo de múltiples aptámeros.

Info

Publication number
MX361451B
MX361451B MX2016003320A MX2016003320A MX361451B MX 361451 B MX361451 B MX 361451B MX 2016003320 A MX2016003320 A MX 2016003320A MX 2016003320 A MX2016003320 A MX 2016003320A MX 361451 B MX361451 B MX 361451B
Authority
MX
Mexico
Prior art keywords
multiaptamer
target detection
aptamer
compositions
target
Prior art date
Application number
MX2016003320A
Other languages
English (en)
Other versions
MX2016003320A (es
Inventor
Gold Larry
Janjic Nebojsa
A Ochsner Urs
S Green Louis
Original Assignee
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Somalogic Inc filed Critical Somalogic Inc
Publication of MX2016003320A publication Critical patent/MX2016003320A/es
Publication of MX361451B publication Critical patent/MX361451B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)

Abstract

La presente invención se refiere a una composición caracterizada porque comprende un primer aptámero, segundo aptámero y un objetivo, en donde el primer aptámero comprende un primer esquema de modificación de pirimidina C-5, el segundo aptámero comprende un segundo esquema de modificación de pirimidina C-5, y en donde el primer esquema de modificación de pirimidina C-5 y el segundo esquema de modificación de pirimidina C-5 son diferentes; en donde el primer esquema demodificación de pirimidina C-5 comprende una 5-(N-bencilcarboxiamida)-2´- deoxiuridina (bndU); y en donde el primer aptámero, segundo aptámero y el objetivo son capaces de formar un complejo ternario.
MX2016003320A 2013-09-24 2014-09-24 Detección objetivo de múltiples aptámeros. MX361451B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361881629P 2013-09-24 2013-09-24
PCT/US2014/057143 WO2015048084A1 (en) 2013-09-24 2014-09-24 Multiaptamer target detection

Publications (2)

Publication Number Publication Date
MX2016003320A MX2016003320A (es) 2016-05-31
MX361451B true MX361451B (es) 2018-12-06

Family

ID=52744404

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016003320A MX361451B (es) 2013-09-24 2014-09-24 Detección objetivo de múltiples aptámeros.

Country Status (13)

Country Link
US (2) US9926566B2 (es)
EP (1) EP3049523B1 (es)
JP (1) JP6506768B2 (es)
KR (1) KR102348283B1 (es)
CN (2) CN105555957A (es)
AU (1) AU2014326975B2 (es)
BR (1) BR112016005957A2 (es)
CA (1) CA2924987C (es)
HK (1) HK1218765A1 (es)
IL (1) IL244284B (es)
MX (1) MX361451B (es)
SG (1) SG11201602063UA (es)
WO (1) WO2015048084A1 (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3049523B1 (en) 2013-09-24 2019-08-07 Somalogic, Inc. Multiaptamer target detection
EP3224361B1 (en) 2014-11-24 2019-06-19 Somalogic, Inc. Nucleic acid compounds for binding growth differentiation factor 11
EP3387152B1 (en) * 2015-12-08 2022-01-26 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
EP3430022B1 (en) 2016-03-14 2020-03-11 Somalogic, Inc. Compounds and methods for the synthesis of 5-(n-protected-tryptaminocarboxyamide)-2'-deoxyuridine phosphoramidite for incorporation into a nucleic acid sequence
KR20190017870A (ko) * 2016-06-17 2019-02-20 프록시머티 바이오사이언시스, 엘엘씨 앱타머 쌍 선택 방법
EP3478843B1 (en) * 2016-07-01 2021-08-04 Somalogic, Inc. Oligonucleotides comprising modified nucleosides
CN110249082B (zh) 2016-12-01 2023-07-07 诺迪勒思附属公司 测定蛋白质的方法
WO2019103131A1 (ja) * 2017-11-24 2019-05-31 国立大学法人 東京大学 アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法
CA3104041A1 (en) * 2018-06-22 2019-12-26 Somalogic, Inc. Improved proteomic multiplex assays
EP4231174A3 (en) 2020-06-11 2023-11-01 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
EP4281774A2 (en) 2021-01-20 2023-11-29 Nautilus Subsidiary, Inc. Methods for biomolecule quantitation
CA3203535A1 (en) 2021-01-21 2022-07-28 Gregory KAPP Systems and methods for biomolecule preparation
AU2022232933A1 (en) 2021-03-11 2023-09-07 Nautilus Subsidiary, Inc. Systems and methods for biomolecule retention
WO2023038859A1 (en) 2021-09-09 2023-03-16 Nautilus Biotechnology, Inc. Characterization and localization of protein modifications
CA3227592A1 (en) 2021-09-22 2023-03-30 Gregory KAPP Methods and systems for determining polypeptide interactions
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
AU2023241893A1 (en) 2022-03-29 2024-09-26 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
US20230360732A1 (en) 2022-04-25 2023-11-09 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
US20240094215A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
WO2024107857A1 (en) 2022-11-15 2024-05-23 Nautilus Subsidiary, Inc. Standard polypeptides
US20240192202A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. Fluidic media for single-analtye arrays
WO2024130000A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
WO2024151373A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
WO2024206122A1 (en) 2023-03-24 2024-10-03 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001257091A1 (en) * 2000-04-18 2001-10-30 Gilead Sciences, Inc. Aptamer based two-site binding assay
US7312325B2 (en) * 2000-09-26 2007-12-25 Duke University RNA aptamers and methods for identifying the same
US20070071744A1 (en) * 2002-06-07 2007-03-29 Gotz Munch Agents which bind to epitopes of glycoprotein VI
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US20100035247A1 (en) 2005-11-04 2010-02-11 U.S. Genomics, Inc. Heterogeneous Assay of Analytes in Solution Using Polymers
WO2008039642A2 (en) 2006-09-26 2008-04-03 Replidyne, Inc. Methods and compounds for treatment of clostridium based infection
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
JP5404620B2 (ja) * 2007-07-17 2014-02-05 ソマロジック・インコーポレーテッド 試験試料の多重化分析
WO2009037264A2 (en) 2007-09-17 2009-03-26 Da Volterra Colonic delivery of antimicrobial agents
US8569252B2 (en) 2009-04-15 2013-10-29 Postech Academy-Industry Foundation Nucleolin specific aptamer and use thereof
US20110256535A1 (en) 2010-02-11 2011-10-20 Intelligent Medical Devices, Inc. Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantification, monitoring, screening and sequencing of clostridium difficile genes encoding toxin b, and/or toxin a and/or binary toxin
GB201002627D0 (en) 2010-02-16 2010-03-31 Loxbridge Res Llp Aptamer based analyte detection method
JP6012591B2 (ja) * 2010-04-12 2016-10-26 ソマロジック, インコーポレイテッドSomaLogic, Inc. β−ＮＧＦに対するアプタマー及びβ−ＮＧＦ介在疾患及び障害の治療におけるその使用
US8785132B2 (en) * 2010-04-23 2014-07-22 Postech Academy-Industry Foundation Aptamer sandwich assays
US20150031585A1 (en) 2011-03-10 2015-01-29 Somalogic, Inc. Multiaptamer Target Detection
KR20140015455A (ko) 2011-03-10 2014-02-06 소마로직, 인크. 클로스트리디움 디피실 진단을 위한 압타머
CA2834200A1 (en) * 2011-04-26 2012-11-01 Regado Biosciences, Inc. A method for manufacturing pegylated oligonucleotides
EP2761289B1 (en) 2011-09-30 2020-02-12 Somalogic, Inc. Cardiovascular risk event prediction and uses thereof
WO2013102101A1 (en) 2011-12-30 2013-07-04 Somalogic, Inc. Aptamers and diagnostic methods for detecting the egf receptor
EP3049523B1 (en) 2013-09-24 2019-08-07 Somalogic, Inc. Multiaptamer target detection

Also Published As

Publication number Publication date
EP3049523B1 (en) 2019-08-07
CA2924987A1 (en) 2015-04-02
CA2924987C (en) 2022-08-16
WO2015048084A1 (en) 2015-04-02
US20180187197A1 (en) 2018-07-05
SG11201602063UA (en) 2016-04-28
US20160237435A1 (en) 2016-08-18
BR112016005957A2 (pt) 2017-09-26
EP3049523A1 (en) 2016-08-03
CN114703193A (zh) 2022-07-05
US9926566B2 (en) 2018-03-27
KR20160058777A (ko) 2016-05-25
HK1218765A1 (zh) 2017-03-10
IL244284B (en) 2019-12-31
CN105555957A (zh) 2016-05-04
IL244284A0 (en) 2016-04-21
US10392621B2 (en) 2019-08-27
AU2014326975B2 (en) 2020-05-07
JP6506768B2 (ja) 2019-04-24
MX2016003320A (es) 2016-05-31
KR102348283B1 (ko) 2022-01-10
EP3049523A4 (en) 2017-05-17
AU2014326975A2 (en) 2017-01-05
JP2016531593A (ja) 2016-10-13
AU2014326975A1 (en) 2016-04-07

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