EA200900212A1 - Элементы нуклеиновых кислот, обладающие регуляторной функцией - Google Patents

Элементы нуклеиновых кислот, обладающие регуляторной функцией

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Publication number
EA200900212A1
EA200900212A1 EA200900212A EA200900212A EA200900212A1 EA 200900212 A1 EA200900212 A1 EA 200900212A1 EA 200900212 A EA200900212 A EA 200900212A EA 200900212 A EA200900212 A EA 200900212A EA 200900212 A1 EA200900212 A1 EA 200900212A1
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EA
Eurasian Patent Office
Prior art keywords
elements
gene
well
expression
sequence
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EA200900212A
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English (en)
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EA016880B1 (ru
Inventor
Барбара Эненкель
Керстин Зауттер
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Бёрингер Ингельхайм Фарма Гмбх Унд Ко. Кг
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Application filed by Бёрингер Ингельхайм Фарма Гмбх Унд Ко. Кг filed Critical Бёрингер Ингельхайм Фарма Гмбх Унд Ко. Кг
Publication of EA200900212A1 publication Critical patent/EA200900212A1/ru
Publication of EA016880B1 publication Critical patent/EA016880B1/ru

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

В заявке описаны последовательности ДНК, прежде всего усиливающие транскрипцию или экспрессию элементы (ТЕ-элементы), а также их применение в экспрессионном векторе в сочетании с энхансером, промотором, геном продукта и селектируемым маркером. В заявке описаны также последовательность SEQ ID NO: 1, а также ТЕ-элементы ТЕ-01, -02, -03, -04, -06, -07, -08, -10, -11 или -12. Наиболее предпочтительными являются ТЕ-06, ТЕ-07 или ТЕ-08, что обусловлено их небольшим размером. Последовательность NO: 1 выведена из области последовательности, расположенной против хода транскрипции относительно кодирующей области гена Ub/S27a из СНО-клеток. ТЕ-элементы вызывают усиление экспрессии гена продукта при стабильной интеграции в эукариотический геном, предпочтительно геном CHO-DG44-клетки. Это позволяет преодолевать хромосомальные позиционные воздействия, защищать от них или устранять их. В результате этого достигается повышение доли высокопродуктивных клеток в трансфектированной смеси, а также абсолютного уровня экспрессии вплоть до 15 раз.
EA200900212A 2006-07-26 2007-06-15 Элементы нуклеиновых кислот, обладающие регуляторной функцией EA016880B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06117862 2006-07-26
PCT/EP2007/055954 WO2008012142A1 (de) 2006-07-26 2007-06-15 Regulatorische nukleinsäureelemente

Publications (2)

Publication Number Publication Date
EA200900212A1 true EA200900212A1 (ru) 2009-10-30
EA016880B1 EA016880B1 (ru) 2012-08-30

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US (3) US20080124760A1 (ru)
EP (1) EP2049671B1 (ru)
JP (1) JP5270544B2 (ru)
KR (1) KR101485853B1 (ru)
CN (1) CN101517085B (ru)
AR (1) AR061472A1 (ru)
AU (1) AU2007278368B2 (ru)
BR (1) BRPI0714594A2 (ru)
CA (1) CA2658595C (ru)
CY (1) CY1113711T1 (ru)
DK (1) DK2049671T3 (ru)
EA (1) EA016880B1 (ru)
ES (1) ES2401654T3 (ru)
HK (1) HK1134107A1 (ru)
IL (1) IL196676A0 (ru)
MX (1) MX2009000781A (ru)
NO (1) NO20090057L (ru)
NZ (1) NZ575115A (ru)
PL (1) PL2049671T3 (ru)
PT (1) PT2049671E (ru)
SI (1) SI2049671T1 (ru)
TW (1) TW200815591A (ru)
WO (1) WO2008012142A1 (ru)

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Publication number Publication date
EP2049671B1 (de) 2012-12-19
US20150337333A1 (en) 2015-11-26
JP2009544293A (ja) 2009-12-17
MX2009000781A (es) 2009-01-29
ES2401654T3 (es) 2013-04-23
SI2049671T1 (sl) 2013-04-30
JP5270544B2 (ja) 2013-08-21
CA2658595C (en) 2016-05-31
US20080124760A1 (en) 2008-05-29
PT2049671E (pt) 2013-01-24
EP2049671A1 (de) 2009-04-22
BRPI0714594A2 (pt) 2013-02-19
AU2007278368B2 (en) 2013-12-19
DK2049671T3 (da) 2013-04-08
NO20090057L (no) 2009-02-16
PL2049671T3 (pl) 2013-05-31
HK1134107A1 (en) 2010-04-16
EA016880B1 (ru) 2012-08-30
KR20090046887A (ko) 2009-05-11
IL196676A0 (en) 2011-08-01
WO2008012142A1 (de) 2008-01-31
NZ575115A (en) 2011-10-28
AU2007278368A1 (en) 2008-01-31
KR101485853B1 (ko) 2015-01-23
US9045776B2 (en) 2015-06-02
AR061472A1 (es) 2008-08-27
US20100311121A1 (en) 2010-12-09
CA2658595A1 (en) 2008-01-31
CY1113711T1 (el) 2016-06-22
CN101517085A (zh) 2009-08-26
CN101517085B (zh) 2013-08-07
TW200815591A (en) 2008-04-01
US9708626B2 (en) 2017-07-18

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