EA200800253A1 - Бессывороточная культуральная среда для получения рекомбинантных гонадотропинов - Google Patents

Бессывороточная культуральная среда для получения рекомбинантных гонадотропинов

Info

Publication number
EA200800253A1
EA200800253A1 EA200800253A EA200800253A EA200800253A1 EA 200800253 A1 EA200800253 A1 EA 200800253A1 EA 200800253 A EA200800253 A EA 200800253A EA 200800253 A EA200800253 A EA 200800253A EA 200800253 A1 EA200800253 A1 EA 200800253A1
Authority
EA
Eurasian Patent Office
Prior art keywords
recombinant
gonadotropines
volume
cultural environment
free
Prior art date
Application number
EA200800253A
Other languages
English (en)
Other versions
EA012840B1 (ru
Inventor
Жан-Пьер Фонта
Поль Дюкоммен
Вероник Депари
Original Assignee
Арес Трейдинг С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Арес Трейдинг С.А. filed Critical Арес Трейдинг С.А.
Publication of EA200800253A1 publication Critical patent/EA200800253A1/ru
Publication of EA012840B1 publication Critical patent/EA012840B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Abstract

Настоящее изобретение относится к области производства рекомбинантных белков. Более конкретно, оно относится к применению бессывороточной культуральной среды, содержащей антиоксидант, для продукции рекомбинантных димерных гонадотропинов. Антиоксидант может быть выбран из группы, состоящей из L-глутатиона, 2-меркаптоэтанола, L-метионина и сочетания аскорбиновой кислоты и (+)-альфа-токоферола.
EA200800253A 2005-07-05 2006-07-04 Применение бессывороточной культуральной среды, содержащей антиоксидант, для снижения окисления рекомбинантных гонадотропинов EA012840B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP05106060 2005-07-05
US69707705P 2005-07-06 2005-07-06
PCT/EP2006/063862 WO2007003640A1 (en) 2005-07-05 2006-07-04 Serum-free culture medium for the production of recombinant gonadotropins

Publications (2)

Publication Number Publication Date
EA200800253A1 true EA200800253A1 (ru) 2008-06-30
EA012840B1 EA012840B1 (ru) 2009-12-30

Family

ID=35134140

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200800253A EA012840B1 (ru) 2005-07-05 2006-07-04 Применение бессывороточной культуральной среды, содержащей антиоксидант, для снижения окисления рекомбинантных гонадотропинов

Country Status (24)

Country Link
US (1) US8093052B2 (ru)
EP (1) EP1899454B1 (ru)
JP (1) JP5059757B2 (ru)
KR (1) KR101346499B1 (ru)
CN (1) CN101258236A (ru)
AR (1) AR054157A1 (ru)
AU (1) AU2006264917B2 (ru)
BR (1) BRPI0612781B8 (ru)
CA (1) CA2613867A1 (ru)
CY (1) CY1112992T1 (ru)
DK (1) DK1899454T3 (ru)
EA (1) EA012840B1 (ru)
ES (1) ES2388875T3 (ru)
HR (1) HRP20120486T1 (ru)
IL (1) IL188558A (ru)
MY (1) MY149621A (ru)
NO (1) NO341475B1 (ru)
PL (1) PL1899454T3 (ru)
PT (1) PT1899454E (ru)
RS (1) RS52468B (ru)
SI (1) SI1899454T1 (ru)
TW (1) TWI369401B (ru)
UA (1) UA91063C2 (ru)
WO (1) WO2007003640A1 (ru)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101264096B (zh) * 2007-03-12 2011-11-09 中国医学科学院药物研究所 人参皂苷Rg1在制备生精产品中的应用
PT2170942E (pt) * 2007-06-28 2013-10-22 Biogenerix Ag Clone celular produtor de fsh
AU2011246504B2 (en) 2010-04-26 2013-09-26 Novartis Ag Improved cell culture medium
CN102296047B (zh) * 2011-08-13 2013-03-13 新疆农垦科学院 利用羔羊超排卵母细胞生产体外胚胎的方法
US10138467B2 (en) * 2013-10-14 2018-11-27 Ares Trading S.A. Medium for high performance mammalian fed-batch cultures
EP3383894B1 (en) * 2015-12-02 2020-05-06 CSL Behring Lengnau AG Improved media for the expression of recombinant vitamin k-dependent proteins
CN105462909B (zh) * 2015-12-31 2019-09-27 哈药集团技术中心 一种高效表达人促卵泡激素的cho细胞的培养方法
CN108795841A (zh) * 2017-05-03 2018-11-13 上海奥浦迈生物科技有限公司 一种st培养基及其应用
CN110042137B (zh) * 2019-05-14 2023-02-28 上海赛迈生物科技有限公司 高密度灌注培养重组cho细胞生产人促卵泡激素的方法、培养基及其应用
US20220204920A1 (en) 2019-05-16 2022-06-30 Formycon Ag Method for reducing methionine oxidation in recombinant proteins
CN114836367A (zh) * 2022-04-28 2022-08-02 上海东富龙生物试剂有限公司 用于hek293细胞培养及腺病毒、腺相关病毒复制扩增的化学成分限定培养基

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DE3110559C2 (de) 1981-03-18 1985-05-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Vollsynthetisches Zellkulturmedium
US4560655A (en) * 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) * 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
FR2600076A1 (fr) * 1986-06-12 1987-12-18 Fond Ctre Nal Transfusion Milieu de culture comportant de l'albumine humaine, procede de preparation d'un produit injectable a partir de ce milieu, produit obtenu et son utilisation, composition obtenue
US5122469A (en) * 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
USH1532H (en) * 1993-11-03 1996-05-07 Genetics Institute, Inc. Adaption of mammalian cell lines to high cell densities
WO1998004680A1 (en) * 1996-07-26 1998-02-05 University Of Manitoba Serum-free medium for growth of anchorage-dependant mammalian cells
EP2221361A3 (en) * 1996-08-30 2011-02-09 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
DE69738806D1 (de) * 1996-10-10 2008-08-14 Invitrogen Corp Tierzellkulturmedium mit pflanzlichen nährstoffen
IL122732A0 (en) * 1997-01-15 1998-08-16 Akzo Nobel Nv Liquid gonadotropin-containing formulation its preparation and a device containing same
WO1999050390A1 (en) * 1998-03-30 1999-10-07 Bionative Ab Methionin containing animal cell culture medium and its use
CA2468971A1 (en) * 2001-12-04 2003-06-12 Mitsubishi Pharma Corporation Method of activating protein
US6756431B2 (en) * 2002-04-09 2004-06-29 Crompton Corporation Heterocyclic tin flame retardants/smoke suppressants and halogen-containing polymer composition containing same
WO2004087213A1 (en) * 2003-04-02 2004-10-14 Ares Trading S.A. Liquid pharmaceutical formulations of fsh and lh together with a non-ionic surfactant
PT1638595E (pt) * 2003-06-20 2013-04-26 Ares Trading Sa Fsh liofilizada / formulações de hl

Also Published As

Publication number Publication date
AU2006264917A1 (en) 2007-01-11
CN101258236A (zh) 2008-09-03
NO341475B1 (no) 2017-11-27
US8093052B2 (en) 2012-01-10
BRPI0612781A2 (pt) 2010-11-30
ES2388875T3 (es) 2012-10-19
CA2613867A1 (en) 2007-01-11
CY1112992T1 (el) 2016-04-13
BRPI0612781B1 (pt) 2020-09-15
US20090124006A1 (en) 2009-05-14
JP5059757B2 (ja) 2012-10-31
MY149621A (en) 2013-09-13
KR20080036599A (ko) 2008-04-28
PT1899454E (pt) 2012-06-25
KR101346499B1 (ko) 2013-12-31
JP2008544758A (ja) 2008-12-11
DK1899454T3 (da) 2012-07-09
RS52468B (en) 2013-02-28
TWI369401B (en) 2012-08-01
IL188558A0 (en) 2008-04-13
EP1899454B1 (en) 2012-05-30
IL188558A (en) 2012-04-30
EA012840B1 (ru) 2009-12-30
AU2006264917B2 (en) 2011-04-28
HRP20120486T1 (hr) 2012-07-31
AR054157A1 (es) 2007-06-06
UA91063C2 (ru) 2010-06-25
NO20080580L (no) 2008-01-31
BRPI0612781B8 (pt) 2021-05-25
WO2007003640A1 (en) 2007-01-11
TW200732473A (en) 2007-09-01
EP1899454A1 (en) 2008-03-19
PL1899454T3 (pl) 2012-10-31
SI1899454T1 (sl) 2012-08-31

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Legal Events

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM