UA91063C2 - Применение безсывороточной культуральной среды для получения рекомбинантных димерных гонадотропинов - Google Patents

Применение безсывороточной культуральной среды для получения рекомбинантных димерных гонадотропинов

Info

Publication number
UA91063C2
UA91063C2 UAA200801394A UAA200801394A UA91063C2 UA 91063 C2 UA91063 C2 UA 91063C2 UA A200801394 A UAA200801394 A UA A200801394A UA A200801394 A UAA200801394 A UA A200801394A UA 91063 C2 UA91063 C2 UA 91063C2
Authority
UA
Ukraine
Prior art keywords
serum
production
culture medium
free culture
recombinant
Prior art date
Application number
UAA200801394A
Other languages
English (en)
Ukrainian (uk)
Inventor
Жан-Пьер Фонта
Поль Дюкоммен
Вероник Депари
Original Assignee
Арес Трейдинг С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Арес Трейдинг С.А. filed Critical Арес Трейдинг С.А.
Publication of UA91063C2 publication Critical patent/UA91063C2/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Reproductive Health (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pregnancy & Childbirth (AREA)
  • Gynecology & Obstetrics (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Данное изобретение относится к отрасли производства рекомбинантных белков. Более конкретно, оно относится к применению безсывороточной культуральной среды, которая содержит антиоксидант, для продукции рекомбинантных димерных гонадотропинов. Антиоксидант может быть выбран из группы, которая состоит из L-глутатиона, 2-меркаптоэтанола, L-метионина и сочетания аскорбиновой кислоты и (+)-альфа-токоферола.
UAA200801394A 2005-07-05 2006-07-04 Применение безсывороточной культуральной среды для получения рекомбинантных димерных гонадотропинов UA91063C2 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP05106060 2005-07-05

Publications (1)

Publication Number Publication Date
UA91063C2 true UA91063C2 (ru) 2010-06-25

Family

ID=35134140

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA200801394A UA91063C2 (ru) 2005-07-05 2006-07-04 Применение безсывороточной культуральной среды для получения рекомбинантных димерных гонадотропинов

Country Status (24)

Country Link
US (1) US8093052B2 (ru)
EP (1) EP1899454B1 (ru)
JP (1) JP5059757B2 (ru)
KR (1) KR101346499B1 (ru)
CN (1) CN101258236A (ru)
AR (1) AR054157A1 (ru)
AU (1) AU2006264917B2 (ru)
BR (1) BRPI0612781B8 (ru)
CA (1) CA2613867A1 (ru)
CY (1) CY1112992T1 (ru)
DK (1) DK1899454T3 (ru)
EA (1) EA012840B1 (ru)
ES (1) ES2388875T3 (ru)
HR (1) HRP20120486T1 (ru)
IL (1) IL188558A (ru)
MY (1) MY149621A (ru)
NO (1) NO341475B1 (ru)
PL (1) PL1899454T3 (ru)
PT (1) PT1899454E (ru)
RS (1) RS52468B (ru)
SI (1) SI1899454T1 (ru)
TW (1) TWI369401B (ru)
UA (1) UA91063C2 (ru)
WO (1) WO2007003640A1 (ru)

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* Cited by examiner, † Cited by third party
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CN101264096B (zh) * 2007-03-12 2011-11-09 中国医学科学院药物研究所 人参皂苷Rg1在制备生精产品中的应用
AU2008267138B9 (en) 2007-06-28 2014-06-05 Theramex HQ UK Limited FSH producing cell clone
MX2012012525A (es) 2010-04-26 2012-11-23 Novartis Ag Medio de cultivo mejorado.
CN102296047B (zh) * 2011-08-13 2013-03-13 新疆农垦科学院 利用羔羊超排卵母细胞生产体外胚胎的方法
KR102418824B1 (ko) * 2013-10-14 2022-07-11 아레스 트레이딩 에스.아. 포유동물의 고성능 유가식 배양을 위한 신규 배지
DK3383894T3 (da) * 2015-12-02 2020-06-02 CSL Behring Lengnau AG Forbedrede medier til eksprimering af rekombinante vitamin k-afhængige proteiner
CN105462909B (zh) * 2015-12-31 2019-09-27 哈药集团技术中心 一种高效表达人促卵泡激素的cho细胞的培养方法
CN108795841A (zh) * 2017-05-03 2018-11-13 上海奥浦迈生物科技有限公司 一种st培养基及其应用
CN110042137B (zh) * 2019-05-14 2023-02-28 上海赛迈生物科技有限公司 高密度灌注培养重组cho细胞生产人促卵泡激素的方法、培养基及其应用
WO2020229584A1 (en) 2019-05-16 2020-11-19 Formycon Ag Method for reducing methionine oxidation in recombinant proteins
CN114836367A (zh) * 2022-04-28 2022-08-02 上海东富龙生物试剂有限公司 用于hek293细胞培养及腺病毒、腺相关病毒复制扩增的化学成分限定培养基

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Also Published As

Publication number Publication date
CY1112992T1 (el) 2016-04-13
PL1899454T3 (pl) 2012-10-31
HRP20120486T1 (hr) 2012-07-31
JP2008544758A (ja) 2008-12-11
DK1899454T3 (da) 2012-07-09
KR101346499B1 (ko) 2013-12-31
KR20080036599A (ko) 2008-04-28
ES2388875T3 (es) 2012-10-19
US20090124006A1 (en) 2009-05-14
NO341475B1 (no) 2017-11-27
BRPI0612781B1 (pt) 2020-09-15
JP5059757B2 (ja) 2012-10-31
TW200732473A (en) 2007-09-01
BRPI0612781B8 (pt) 2021-05-25
BRPI0612781A2 (pt) 2010-11-30
IL188558A0 (en) 2008-04-13
EP1899454A1 (en) 2008-03-19
TWI369401B (en) 2012-08-01
CN101258236A (zh) 2008-09-03
EA200800253A1 (ru) 2008-06-30
NO20080580L (no) 2008-01-31
US8093052B2 (en) 2012-01-10
SI1899454T1 (sl) 2012-08-31
AR054157A1 (es) 2007-06-06
AU2006264917A1 (en) 2007-01-11
WO2007003640A1 (en) 2007-01-11
EP1899454B1 (en) 2012-05-30
RS52468B (en) 2013-02-28
AU2006264917B2 (en) 2011-04-28
MY149621A (en) 2013-09-13
IL188558A (en) 2012-04-30
CA2613867A1 (en) 2007-01-11
PT1899454E (pt) 2012-06-25
EA012840B1 (ru) 2009-12-30

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