HRP20120486T1 - Kultivirani medij bez seruma za proizvodnju rekombinantnih gonadotropina - Google Patents
Kultivirani medij bez seruma za proizvodnju rekombinantnih gonadotropina Download PDFInfo
- Publication number
- HRP20120486T1 HRP20120486T1 HRP20120486TT HRP20120486T HRP20120486T1 HR P20120486 T1 HRP20120486 T1 HR P20120486T1 HR P20120486T T HRP20120486T T HR P20120486TT HR P20120486 T HRP20120486 T HR P20120486T HR P20120486 T1 HRP20120486 T1 HR P20120486T1
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- Prior art keywords
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Links
- 102000006771 Gonadotropins Human genes 0.000 title claims abstract 5
- 108010086677 Gonadotropins Proteins 0.000 title claims abstract 5
- 239000002622 gonadotropin Substances 0.000 title claims abstract 5
- 238000004519 manufacturing process Methods 0.000 title claims abstract 3
- 229940094892 gonadotropins Drugs 0.000 title 1
- 239000004017 serum-free culture medium Substances 0.000 title 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract 6
- 239000002609 medium Substances 0.000 claims abstract 5
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims abstract 5
- RWSXRVCMGQZWBV-PHDIDXHHSA-N L-Glutathione Natural products OC(=O)[C@H](N)CCC(=O)N[C@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-PHDIDXHHSA-N 0.000 claims abstract 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims abstract 3
- 239000003963 antioxidant agent Substances 0.000 claims abstract 3
- 230000003078 antioxidant effect Effects 0.000 claims abstract 3
- 235000006708 antioxidants Nutrition 0.000 claims abstract 3
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract 3
- 229960005070 ascorbic acid Drugs 0.000 claims abstract 3
- 239000011668 ascorbic acid Substances 0.000 claims abstract 3
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract 3
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 claims abstract 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims 2
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 claims 2
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 claims 2
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims 2
- 229930195722 L-methionine Natural products 0.000 claims 2
- 229940028334 follicle stimulating hormone Drugs 0.000 claims 2
- 229960004452 methionine Drugs 0.000 claims 2
- 239000002076 α-tocopherol Substances 0.000 claims 2
- 235000004835 α-tocopherol Nutrition 0.000 claims 2
- GVJHHUAWPYXKBD-QLVXXPONSA-N (S,R,R)-alpha-tocopherol Chemical compound [H][C@@](C)(CCCC(C)C)CCC[C@@]([H])(C)CCC[C@@]1(C)CCC2=C(O1)C(C)=C(C)C(O)=C2C GVJHHUAWPYXKBD-QLVXXPONSA-N 0.000 claims 1
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 abstract 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 abstract 1
- 229930182817 methionine Natural products 0.000 abstract 1
- 239000012679 serum free medium Substances 0.000 abstract 1
- 235000010384 tocopherol Nutrition 0.000 abstract 1
- 229960001295 tocopherol Drugs 0.000 abstract 1
- 229930003799 tocopherol Natural products 0.000 abstract 1
- 239000011732 tocopherol Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/44—Thiols, e.g. mercaptoethanol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2511/00—Cells for large scale production
Abstract
Uporaba kultiviranog medija bez seruma za smanjivanje razina oksidiranih oblika rekombinantnog dimernog gonadotropina tijekom njegovog proizvodnog procesa naznačena time da navedeni kultivirani medijsadrži antioksidans koji je odabran iz skupine koja sadrži L glutation u koncentraciji u rasponu od oko do oko mg L merkaptoetanol u koncentraciji u rasponu od oko do oko mg L L metionin u koncentraciji u rasponu od oko do okomg L ikombinaciju askorbinske kiseline u koncentraciji u rasponu od oko do oko mg L i ofalfa tokoferol u koncentraciji u rasponu od okodo oko mg L Patent sadrži još patentnih zahtjeva
Claims (6)
1. Uporaba kultiviranog medija bez seruma za smanjivanje razina oksidiranih oblika rekombinantnog dimernog gonadotropina tijekom njegovog proizvodnog procesa naznačena time da navedeni kultivirani medij sadrži antioksidans koji je odabran iz skupine koja sadrži:
L-glutation u koncentraciji u rasponu od oko 1 do oko 20 mg/L;
2-merkaptoetanol u koncentraciji u rasponu od oko 5 do oko 15 mg/L;
L-metionin u koncentraciji u rasponu od oko 200 do oko 400 mg/L; i
kombinaciju askorbinske kiseline u koncentraciji u rasponu od oko 10 do oko 50 mg/L i of (+)-alfa-tokoferol u koncentraciji u rasponu od oko 5 do oko 25 mg/L.
2. Uporaba prema zahtjevu 1, naznačena time da je navedeni antioksidans odabran iz skupine koja sadrži:
L-glutation u koncentraciji od oko 3 mg/L;
2-merkaptoetanol u koncentraciji od oko 10 mg/L;
L-metionin u koncentraciji od oko 250 mg/L; i
kombinaciju askorbinske kiseline u koncentraciji od oko 30 mg/L, te
(+)-alfa-tokoferol u koncentraciji od oko 14 mg/L.
3. Uporaba prema zahtjevu 1 ili 2, naznačena time da navedeni kultivirani medij je kemijski definiran medij.
4. Uporaba prema bilo kojem od zahtjeva 1 do 3, naznačena time da navedeni kultivirani medij je odabran iz skupine koja sadrži SFM 90, SFM 90.1, SupMed300, DMEM, DMEM/F12, SFM CHO 3a, CHP PFM, ProCHO 5, EX-CELL, CHO-CD3, CHO III PFM, CHO-S-SFM II, CHO-DHFR, SFM4CHO, Ultra CHO, HyQ PF CHO, HyQ SFX CHO, HyQ CDM4CHO, IS CHO-CD, IS CHO-V, i njihovi derivati.
5. Uporaba prema zahtjevu 4, naznačena time da navedeni dimerni gonadotropin je folikul stimulirajući hormon (FSH).
6. Uporaba prema bilo kojem od zahtjeva 1 do 5, naznačena time da navedeni rekombinantni dimerni gonadotropin je proizveden u stanicama jajnika kineskog hrčka (CHO).
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05106060 | 2005-07-05 | ||
| US69707705P | 2005-07-06 | 2005-07-06 | |
| PCT/EP2006/063862 WO2007003640A1 (en) | 2005-07-05 | 2006-07-04 | Serum-free culture medium for the production of recombinant gonadotropins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20120486T1 true HRP20120486T1 (hr) | 2012-07-31 |
Family
ID=35134140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20120486TT HRP20120486T1 (hr) | 2005-07-05 | 2012-06-11 | Kultivirani medij bez seruma za proizvodnju rekombinantnih gonadotropina |
Country Status (24)
| Country | Link |
|---|---|
| US (1) | US8093052B2 (hr) |
| EP (1) | EP1899454B1 (hr) |
| JP (1) | JP5059757B2 (hr) |
| KR (1) | KR101346499B1 (hr) |
| CN (1) | CN101258236A (hr) |
| AR (1) | AR054157A1 (hr) |
| AU (1) | AU2006264917B2 (hr) |
| BR (1) | BRPI0612781B8 (hr) |
| CA (1) | CA2613867A1 (hr) |
| CY (1) | CY1112992T1 (hr) |
| DK (1) | DK1899454T3 (hr) |
| EA (1) | EA012840B1 (hr) |
| ES (1) | ES2388875T3 (hr) |
| HR (1) | HRP20120486T1 (hr) |
| IL (1) | IL188558A (hr) |
| MY (1) | MY149621A (hr) |
| NO (1) | NO341475B1 (hr) |
| PL (1) | PL1899454T3 (hr) |
| PT (1) | PT1899454E (hr) |
| RS (1) | RS52468B (hr) |
| SI (1) | SI1899454T1 (hr) |
| TW (1) | TWI369401B (hr) |
| UA (1) | UA91063C2 (hr) |
| WO (1) | WO2007003640A1 (hr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101264096B (zh) * | 2007-03-12 | 2011-11-09 | 中国医学科学院药物研究所 | 人参皂苷Rg1在制备生精产品中的应用 |
| PT2170942E (pt) * | 2007-06-28 | 2013-10-22 | Biogenerix Ag | Clone celular produtor de fsh |
| AU2011246504B2 (en) | 2010-04-26 | 2013-09-26 | Novartis Ag | Improved cell culture medium |
| CN102296047B (zh) * | 2011-08-13 | 2013-03-13 | 新疆农垦科学院 | 利用羔羊超排卵母细胞生产体外胚胎的方法 |
| US10138467B2 (en) * | 2013-10-14 | 2018-11-27 | Ares Trading S.A. | Medium for high performance mammalian fed-batch cultures |
| EP3383894B1 (en) * | 2015-12-02 | 2020-05-06 | CSL Behring Lengnau AG | Improved media for the expression of recombinant vitamin k-dependent proteins |
| CN105462909B (zh) * | 2015-12-31 | 2019-09-27 | 哈药集团技术中心 | 一种高效表达人促卵泡激素的cho细胞的培养方法 |
| CN108795841A (zh) * | 2017-05-03 | 2018-11-13 | 上海奥浦迈生物科技有限公司 | 一种st培养基及其应用 |
| CN110042137B (zh) * | 2019-05-14 | 2023-02-28 | 上海赛迈生物科技有限公司 | 高密度灌注培养重组cho细胞生产人促卵泡激素的方法、培养基及其应用 |
| US20220204920A1 (en) | 2019-05-16 | 2022-06-30 | Formycon Ag | Method for reducing methionine oxidation in recombinant proteins |
| CN114836367A (zh) * | 2022-04-28 | 2022-08-02 | 上海东富龙生物试剂有限公司 | 用于hek293细胞培养及腺病毒、腺相关病毒复制扩增的化学成分限定培养基 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3110559C2 (de) | 1981-03-18 | 1985-05-09 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Vollsynthetisches Zellkulturmedium |
| US4560655A (en) * | 1982-12-16 | 1985-12-24 | Immunex Corporation | Serum-free cell culture medium and process for making same |
| US4657866A (en) * | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| FR2600076A1 (fr) * | 1986-06-12 | 1987-12-18 | Fond Ctre Nal Transfusion | Milieu de culture comportant de l'albumine humaine, procede de preparation d'un produit injectable a partir de ce milieu, produit obtenu et son utilisation, composition obtenue |
| US5122469A (en) * | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| USH1532H (en) * | 1993-11-03 | 1996-05-07 | Genetics Institute, Inc. | Adaption of mammalian cell lines to high cell densities |
| WO1998004680A1 (en) * | 1996-07-26 | 1998-02-05 | University Of Manitoba | Serum-free medium for growth of anchorage-dependant mammalian cells |
| EP2221361A3 (en) * | 1996-08-30 | 2011-02-09 | Life Technologies Corporation | Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium |
| DE69738806D1 (de) * | 1996-10-10 | 2008-08-14 | Invitrogen Corp | Tierzellkulturmedium mit pflanzlichen nährstoffen |
| IL122732A0 (en) * | 1997-01-15 | 1998-08-16 | Akzo Nobel Nv | Liquid gonadotropin-containing formulation its preparation and a device containing same |
| WO1999050390A1 (en) * | 1998-03-30 | 1999-10-07 | Bionative Ab | Methionin containing animal cell culture medium and its use |
| CA2468971A1 (en) * | 2001-12-04 | 2003-06-12 | Mitsubishi Pharma Corporation | Method of activating protein |
| US6756431B2 (en) * | 2002-04-09 | 2004-06-29 | Crompton Corporation | Heterocyclic tin flame retardants/smoke suppressants and halogen-containing polymer composition containing same |
| WO2004087213A1 (en) * | 2003-04-02 | 2004-10-14 | Ares Trading S.A. | Liquid pharmaceutical formulations of fsh and lh together with a non-ionic surfactant |
| PT1638595E (pt) * | 2003-06-20 | 2013-04-26 | Ares Trading Sa | Fsh liofilizada / formulações de hl |
-
2006
- 2006-06-20 TW TW095121976A patent/TWI369401B/zh active
- 2006-07-03 MY MYPI20063160A patent/MY149621A/en unknown
- 2006-07-04 CA CA002613867A patent/CA2613867A1/en not_active Abandoned
- 2006-07-04 US US11/994,885 patent/US8093052B2/en active Active
- 2006-07-04 PT PT06764058T patent/PT1899454E/pt unknown
- 2006-07-04 KR KR1020087003007A patent/KR101346499B1/ko active IP Right Grant
- 2006-07-04 EP EP06764058A patent/EP1899454B1/en active Active
- 2006-07-04 UA UAA200801394A patent/UA91063C2/ru unknown
- 2006-07-04 ES ES06764058T patent/ES2388875T3/es active Active
- 2006-07-04 DK DK06764058.1T patent/DK1899454T3/da active
- 2006-07-04 EA EA200800253A patent/EA012840B1/ru not_active IP Right Cessation
- 2006-07-04 BR BRPI0612781A patent/BRPI0612781B8/pt active IP Right Grant
- 2006-07-04 CN CNA2006800322795A patent/CN101258236A/zh active Pending
- 2006-07-04 SI SI200631359T patent/SI1899454T1/sl unknown
- 2006-07-04 PL PL06764058T patent/PL1899454T3/pl unknown
- 2006-07-04 RS RS20120351A patent/RS52468B/en unknown
- 2006-07-04 WO PCT/EP2006/063862 patent/WO2007003640A1/en active Application Filing
- 2006-07-04 JP JP2008519930A patent/JP5059757B2/ja active Active
- 2006-07-04 AU AU2006264917A patent/AU2006264917B2/en active Active
- 2006-07-04 AR ARP060102879A patent/AR054157A1/es not_active Application Discontinuation
-
2008
- 2008-01-03 IL IL188558A patent/IL188558A/en active IP Right Grant
- 2008-01-31 NO NO20080580A patent/NO341475B1/no unknown
-
2012
- 2012-06-11 HR HRP20120486TT patent/HRP20120486T1/hr unknown
- 2012-07-09 CY CY20121100609T patent/CY1112992T1/el unknown
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