UA103598C2 - Клон клеток, которые продуцируют fsh - Google Patents
Клон клеток, которые продуцируют fsh Download PDFInfo
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- UA103598C2 UA103598C2 UAA201000813A UAA201000813A UA103598C2 UA 103598 C2 UA103598 C2 UA 103598C2 UA A201000813 A UAA201000813 A UA A201000813A UA A201000813 A UAA201000813 A UA A201000813A UA 103598 C2 UA103598 C2 UA 103598C2
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- nucleic acid
- chain
- acid sequence
- human
- cells
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Links
- 210000004368 gonadotroph Anatomy 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract description 110
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 78
- 210000004027 cell Anatomy 0.000 abstract description 72
- 108020004707 nucleic acids Proteins 0.000 abstract description 37
- 102000039446 nucleic acids Human genes 0.000 abstract description 37
- 210000004978 chinese hamster ovary cell Anatomy 0.000 abstract description 11
- 108700010070 Codon Usage Proteins 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000004114 suspension culture Methods 0.000 abstract description 2
- 102000003864 Human Follicle Stimulating Hormone Human genes 0.000 abstract 3
- 108010082302 Human Follicle Stimulating Hormone Proteins 0.000 abstract 3
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 abstract 1
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 abstract 1
- 229940028334 follicle stimulating hormone Drugs 0.000 abstract 1
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- 238000004113 cell culture Methods 0.000 description 9
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- 239000003814 drug Substances 0.000 description 3
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
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- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 2
- 101100237844 Mus musculus Mmp19 gene Proteins 0.000 description 2
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- 229960000485 methotrexate Drugs 0.000 description 2
- 230000010412 perfusion Effects 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 2
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 2
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- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- OZAIFHULBGXAKX-UHFFFAOYSA-N 2-(2-cyanopropan-2-yldiazenyl)-2-methylpropanenitrile Chemical compound N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 description 1
- WUBBRNOQWQTFEX-UHFFFAOYSA-N 4-aminosalicylic acid Chemical compound NC1=CC=C(C(O)=O)C(O)=C1 WUBBRNOQWQTFEX-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000611750 Coregonus kiyi Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108020005350 Initiator Codon Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 244000232488 Jessenia polycarpa Species 0.000 description 1
- 235000002407 Jessenia polycarpa Nutrition 0.000 description 1
- 241001175904 Labeo bata Species 0.000 description 1
- 101500021084 Locusta migratoria 5 kDa peptide Proteins 0.000 description 1
- 101000966481 Mus musculus Dihydrofolate reductase Proteins 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 239000005700 Putrescine Substances 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 108010084455 Zeocin Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
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- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- GIXWDMTZECRIJT-UHFFFAOYSA-N aurintricarboxylic acid Chemical compound C1=CC(=O)C(C(=O)O)=CC1=C(C=1C=C(C(O)=CC=1)C(O)=O)C1=CC=C(O)C(C(O)=O)=C1 GIXWDMTZECRIJT-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 244000309466 calf Species 0.000 description 1
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- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000006151 minimal media Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- CWCMIVBLVUHDHK-ZSNHEYEWSA-N phleomycin D1 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC[C@@H](N=1)C=1SC=C(N=1)C(=O)NCCCCNC(N)=N)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C CWCMIVBLVUHDHK-ZSNHEYEWSA-N 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 229950010131 puromycin Drugs 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
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- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Reproductive Health (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Изобретение относится к молекулам нуклеиновых кислот, которые содержат последовательность нуклеиновой кислоты, которая кодирует α- и β-цепи фолликулостимулирующего гормона (FSH) человека, соответственно, которые модифицированы относительно использования кодонов в клетках СНО, рекомбинантной молекуле нуклеиновой кислоты, которая содержит такие последовательности нуклеинових кислот, и клеткам-хозяевам, которые содержат такие рекомбинантные молекулы нуклеиновых кислот, а также их применению при получении рекомбинантного FSH человека, способу получения клеток-хозяев, которые экспрессируют FSH.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07111257 | 2007-06-28 | ||
| PCT/EP2008/058274 WO2009000913A1 (en) | 2007-06-28 | 2008-06-27 | Fsh producing cell clone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA103598C2 true UA103598C2 (ru) | 2013-11-11 |
Family
ID=39745099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAA201000813A UA103598C2 (ru) | 2007-06-28 | 2008-06-27 | Клон клеток, которые продуцируют fsh |
Country Status (24)
| Country | Link |
|---|---|
| US (1) | US8318457B2 (ru) |
| EP (2) | EP2492280A1 (ru) |
| JP (1) | JP5570980B2 (ru) |
| KR (1) | KR101522217B1 (ru) |
| CN (1) | CN101720332B (ru) |
| AU (1) | AU2008267138B9 (ru) |
| BR (1) | BRPI0813913B1 (ru) |
| CA (1) | CA2690844C (ru) |
| CY (1) | CY1114463T1 (ru) |
| DK (1) | DK2170942T3 (ru) |
| EA (1) | EA022993B1 (ru) |
| ES (1) | ES2432183T3 (ru) |
| HK (1) | HK1140782A1 (ru) |
| HR (1) | HRP20130921T1 (ru) |
| IL (1) | IL202280A (ru) |
| MX (1) | MX2010000385A (ru) |
| NZ (1) | NZ581702A (ru) |
| PL (1) | PL2170942T3 (ru) |
| PT (1) | PT2170942E (ru) |
| RS (1) | RS52976B (ru) |
| SI (1) | SI2170942T1 (ru) |
| UA (1) | UA103598C2 (ru) |
| WO (1) | WO2009000913A1 (ru) |
| ZA (1) | ZA200908168B (ru) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110433136A (zh) | 2008-02-08 | 2019-11-12 | 泰哈麦克斯总部英国有限公司 | 促卵泡激素的液体制剂 |
| KR101761168B1 (ko) * | 2009-04-01 | 2017-07-25 | 바이오제너릭스 게엠베하 | 재조합 ｆｓｈ 정제 방법 |
| EP2325194A1 (en) | 2009-11-24 | 2011-05-25 | Glycotope GmbH | Process for the purification of glycoproteins |
| JP6279466B2 (ja) * | 2012-04-27 | 2018-02-14 | Ｊｃｒファーマ株式会社 | 新規な発現ベクター |
| CN103539860B (zh) * | 2013-11-01 | 2014-12-03 | 广州优联康医药科技有限公司 | 一种长效重组人促卵泡激素融合蛋白 |
| CN103554269B (zh) * | 2013-11-01 | 2015-02-11 | 广州联康生物科技有限公司 | 重组猪促卵泡激素融合蛋白 |
| CN103539861B (zh) * | 2013-11-01 | 2015-02-18 | 广州联康生物科技有限公司 | 长效重组人促卵泡激素融合蛋白 |
| CN105462909B (zh) * | 2015-12-31 | 2019-09-27 | 哈药集团技术中心 | 一种高效表达人促卵泡激素的cho细胞的培养方法 |
| CN107460206B (zh) * | 2017-09-11 | 2020-05-01 | 深圳市深研生物科技有限公司 | 人fsh的重组表达载体、重组细胞株及重组人fsh的制备方法 |
| CN111349154A (zh) * | 2018-12-21 | 2020-06-30 | 江苏众红生物工程创药研究院有限公司 | 一种重组人促卵泡激素及其制备方法和药物应用 |
| CN110812366B (zh) * | 2019-11-18 | 2023-11-17 | 珠海丽凡达生物技术有限公司 | 一种可用于激素补充的mRNA药物及其制备方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4923805A (en) | 1983-11-02 | 1990-05-08 | Integrated Genetics, Inc. | Fsh |
| IT1206302B (it) | 1987-06-26 | 1989-04-14 | Serono Cesare Ist Ricerca | Ormone follicolo-stimolante urinario |
| CA2018676C (en) | 1989-06-20 | 2003-05-06 | Christie A. Kelton | Novel heteropolymeric protein production methods |
| CA2223125A1 (en) | 1995-02-17 | 1996-08-22 | Stichting Instituut Voor Dierhouderij En Diergezondheid | Production of biologically active recombinant bovine follicle stimulating hormone (recbfsh) in the baculovirus expression system |
| TW518235B (en) | 1997-01-15 | 2003-01-21 | Akzo Nobel Nv | A gonadotropin-containing pharmaceutical composition with improved stability on prolong storage |
| PL346127A1 (en) | 1998-07-23 | 2002-01-28 | Lilly Co Eli | Fsh and fsh variant formulations, products and methods |
| DE60034117T2 (de) | 1999-04-16 | 2007-12-13 | Instituto Massone S.A. | Verfahren zur herstelllung von gonadotropin-zusammensetzungen |
| US20020127652A1 (en) | 2000-02-11 | 2002-09-12 | Schambye Hans Thalsgard | Follicle stimulating hormones |
| WO2001058493A1 (en) * | 2000-02-11 | 2001-08-16 | Maxygen Aps | Conjugates of follicle stimulating hormones |
| CN1694958A (zh) * | 2002-09-13 | 2005-11-09 | 昆士兰大学 | 以密码子翻译效率为基础的基因表达系统 |
| KR100533794B1 (ko) * | 2003-10-02 | 2005-12-07 | 주식회사 프로젠 | 인간 난포자극 호르몬을 대량으로 생산하는 방법 |
| CN100554277C (zh) | 2003-12-22 | 2009-10-28 | 阿雷斯贸易股份有限公司 | Fsh的纯化方法 |
| CN101087805B (zh) | 2004-11-09 | 2012-05-23 | 阿雷斯贸易股份有限公司 | Fsh的纯化方法 |
| TWI369401B (en) * | 2005-07-05 | 2012-08-01 | Ares Trading Sa | Serum-free culture medium for the production of recombinant gonadotropins |
| US20070281883A1 (en) | 2006-06-05 | 2007-12-06 | Hanna Rosenfeld | Development of follicle stimulating hormone agonists and antagonists in fish |
-
2008
- 2008-06-27 EP EP12169533A patent/EP2492280A1/en not_active Withdrawn
- 2008-06-27 EP EP08774439.7A patent/EP2170942B1/en active Active
- 2008-06-27 BR BRPI0813913-0A patent/BRPI0813913B1/pt active IP Right Grant
- 2008-06-27 UA UAA201000813A patent/UA103598C2/ru unknown
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- 2008-06-27 WO PCT/EP2008/058274 patent/WO2009000913A1/en active Application Filing
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