EA200700330A1 - Биохимический синтез 1,4-бутандиамина - Google Patents

Биохимический синтез 1,4-бутандиамина

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Publication number
EA200700330A1
EA200700330A1 EA200700330A EA200700330A EA200700330A1 EA 200700330 A1 EA200700330 A1 EA 200700330A1 EA 200700330 A EA200700330 A EA 200700330A EA 200700330 A EA200700330 A EA 200700330A EA 200700330 A1 EA200700330 A1 EA 200700330A1
Authority
EA
Eurasian Patent Office
Prior art keywords
encoding gene
activity
increased
ornithine decarboxylase
overexpression
Prior art date
Application number
EA200700330A
Other languages
English (en)
Other versions
EA011232B1 (ru
Inventor
Катрин Эппельман
Петрус Мартинус Матеус Носсин
Сусанна Мария Кремер
Марсель Герхардус Вубболтс
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA200700330A1 publication Critical patent/EA200700330A1/ru
Publication of EA011232B1 publication Critical patent/EA011232B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01053—N-carbamoylputrescine amidase (3.5.1.53)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/03—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amidines (3.5.3)
    • C12Y305/03011—Agmatinase (3.5.3.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/03—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amidines (3.5.3)
    • C12Y305/03012—Agmatine deiminase (3.5.3.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01017—Ornithine decarboxylase (4.1.1.17)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Изобретение относится к новому способу биохимического синтеза 1,4-бутандиамина в микроорганизме, имеющем повышенный уровень активности ортининдекарбоксилазы (повышенная активность ODC) по сравнению с нативным уровнем орнитиндекарбоксилазы, где повышенную активность ODC получают посредством сверхэкспрессии гена, кодирующего орнитиндекарбоксилазу с увеличенной транскрипционной и/или трансляционной эффективностью и где производимый в микроорганизме 1,4-бутандиамина секретируется в среду ферментации и извлекается из среды ферментации. В предпочтительных воплощениях повышенная ферментативная активность получена путем сверхэкспрессии либо (i) гена speA, кодирующего аргининдекарбоксилазу, и гена speB, кодирующего агматиназу; (ii) гена speA, кодирующего аргининдекарбоксилазу, и гена aguA, кодирующего агматиниминогидролазу, и гена aguB, кодирующего N-карбамоилпутресцинамидогидролазу, и необязательно гена speB, кодирующего агматиназу. Изобретение относится также к векторам, плазмидам и к хозяевам, несущим при повышенном уровне активности одну или более упомянутых ферментативных активностей.
EA200700330A 2004-07-15 2005-07-11 Биохимический синтез 1,4-бутандиамина EA011232B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04077047 2004-07-15
PCT/EP2005/007608 WO2006005604A1 (en) 2004-07-15 2005-07-11 Biochemical synthesis of 1,4-butanediamine

Publications (2)

Publication Number Publication Date
EA200700330A1 true EA200700330A1 (ru) 2007-06-29
EA011232B1 EA011232B1 (ru) 2009-02-27

Family

ID=34928371

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200700330A EA011232B1 (ru) 2004-07-15 2005-07-11 Биохимический синтез 1,4-бутандиамина

Country Status (18)

Country Link
US (2) US9523083B2 (ru)
EP (2) EP2236613B1 (ru)
JP (2) JP5010470B2 (ru)
CN (1) CN101006183B (ru)
AT (1) ATE504658T1 (ru)
AU (1) AU2005261862B2 (ru)
BR (1) BRPI0513362A (ru)
CA (1) CA2571531C (ru)
DE (1) DE602005027356D1 (ru)
EA (1) EA011232B1 (ru)
MX (1) MX2007000565A (ru)
MY (1) MY148119A (ru)
NZ (1) NZ552628A (ru)
PL (2) PL1781799T3 (ru)
TW (1) TWI354026B (ru)
UA (1) UA91686C2 (ru)
WO (1) WO2006005604A1 (ru)
ZA (1) ZA200700072B (ru)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL1781799T3 (pl) * 2004-07-15 2011-07-29 Dsm Ip Assets Bv Biochemiczna synteza 1,4-butanodiaminy
ES2589378T3 (es) * 2008-04-10 2016-11-14 Korea Advanced Institute Of Science And Technology Microorganismo mutante con capacidad elevada para producir putrescina, y preparación de putrescina usando el mismo
ES2749423T3 (es) * 2009-05-07 2020-03-20 Genomatica Inc Microorganismos y métodos para la biosíntesis de adipato, hexametilendiamina y ácido 6-aminocaproico
KR101694572B1 (ko) * 2009-07-24 2017-01-09 디에스엠 아이피 어셋츠 비.브이. Ｎ-아실 또는 ｎ-구아니딜 보호된 1,4-뷰테인다이아민 전구체를 통한 1,4-뷰테인다이아민의 제조 방법
SG10201610621XA (en) * 2012-06-19 2017-02-27 Univ Nanyang Tech An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids
CN103451123B (zh) * 2013-06-08 2015-10-21 江苏省农业科学院 溶酪大球菌及其制备方法和应用
JP2017158434A (ja) * 2014-07-22 2017-09-14 協同乳業株式会社 バクテリア混合培養によるプトレッシン製造方法
CN104152478A (zh) * 2014-07-31 2014-11-19 洛阳华荣生物技术有限公司 一种生物转化联产d-精氨酸和胍基丁胺的方法
KR102246288B1 (ko) 2020-08-13 2021-04-29 씨제이제일제당 주식회사 퓨트레신 생산 미생물 및 이를 이용한 퓨트레신 생산방법
CN112522171A (zh) * 2020-12-18 2021-03-19 浙江中山化工集团股份有限公司 一种工程菌、含鸟氨酸溶液的处理方法及试剂盒
CN113061562B (zh) * 2021-03-22 2022-09-27 江南大学 一种利用钝齿棒杆菌发酵生产1,4-丁二胺的方法
WO2023157816A1 (ja) * 2022-02-15 2023-08-24 東レ株式会社 ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2130948A (en) 1937-04-09 1938-09-20 Du Pont Synthetic fiber
FR2729775B1 (fr) 1995-01-23 1997-02-21 France Telecom Terminal a lecteur de carte et procede de traitement multi-applicatif d'un tel terminal
ES2088826B1 (es) * 1995-02-10 1997-06-01 Taguidell Sociedad Limitada Procedimiento de obtencion de las diaminas putrescina y cadaverina a partir de productos naturales tratados, su uso como aditivo en abonos, y abono correspondiente.
FI980551A (fi) * 1998-03-11 1999-09-12 Valtion Teknillinen Transformoidut mikro-organismit, joilla on parannettuja ominaisuuksia
AU6464100A (en) * 1999-08-10 2001-03-05 John Innes Centre Polyamine accumulation in plants
DE10161412A1 (de) 2001-12-13 2003-07-03 Aventis Pharma Gmbh Verfahren zur Bestimmung der Aktivität der Ornithin-Decarboxylase sowie zur Identifizierung von Effektoren der Ornithin-Decarboxylase-Aktivität
PL1781799T3 (pl) * 2004-07-15 2011-07-29 Dsm Ip Assets Bv Biochemiczna synteza 1,4-butanodiaminy

Also Published As

Publication number Publication date
PL2236613T3 (pl) 2015-11-30
US20090275093A1 (en) 2009-11-05
CA2571531C (en) 2016-02-16
WO2006005604A1 (en) 2006-01-19
NZ552628A (en) 2009-09-25
ZA200700072B (en) 2008-05-28
PL1781799T3 (pl) 2011-07-29
EP2236613B1 (en) 2015-05-27
ATE504658T1 (de) 2011-04-15
CA2571531A1 (en) 2006-01-19
MY148119A (en) 2013-02-28
TWI354026B (en) 2011-12-11
JP5010470B2 (ja) 2012-08-29
JP2012130343A (ja) 2012-07-12
BRPI0513362A (pt) 2008-05-06
US9523083B2 (en) 2016-12-20
AU2005261862A1 (en) 2006-01-19
EP2236613A1 (en) 2010-10-06
MX2007000565A (es) 2007-04-02
JP2008505652A (ja) 2008-02-28
CN101006183A (zh) 2007-07-25
UA91686C2 (en) 2010-08-25
DE602005027356D1 (de) 2011-05-19
TW200613558A (en) 2006-05-01
AU2005261862B2 (en) 2010-08-26
CN101006183B (zh) 2011-07-27
US20160319311A1 (en) 2016-11-03
EP1781799B1 (en) 2011-04-06
EA011232B1 (ru) 2009-02-27
EP1781799A1 (en) 2007-05-09

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU