SG10201610621XA - An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids - Google Patents

An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids

Info

Publication number
SG10201610621XA
SG10201610621XA SG10201610621XA SG10201610621XA SG10201610621XA SG 10201610621X A SG10201610621X A SG 10201610621XA SG 10201610621X A SG10201610621X A SG 10201610621XA SG 10201610621X A SG10201610621X A SG 10201610621XA SG 10201610621X A SG10201610621X A SG 10201610621XA
Authority
SG
Singapore
Prior art keywords
conversion
cell density
expression construct
sensing cell
hydroxycinnamic acids
Prior art date
Application number
SG10201610621XA
Inventor
Matthew Wook Chang
Tat Ming Samuel Lo
Chueh Loo Poh
Original Assignee
Univ Nanyang Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nanyang Tech filed Critical Univ Nanyang Tech
Publication of SG10201610621XA publication Critical patent/SG10201610621XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01012—4-Coumarate-CoA ligase (6.2.1.12)
SG10201610621XA 2012-06-19 2013-06-12 An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids SG10201610621XA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261661593P 2012-06-19 2012-06-19

Publications (1)

Publication Number Publication Date
SG10201610621XA true SG10201610621XA (en) 2017-02-27

Family

ID=49769121

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201610621XA SG10201610621XA (en) 2012-06-19 2013-06-12 An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids
SG11201408353QA SG11201408353QA (en) 2012-06-19 2013-06-12 An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201408353QA SG11201408353QA (en) 2012-06-19 2013-06-12 An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids

Country Status (4)

Country Link
US (1) US10683521B2 (en)
EP (1) EP2877582B1 (en)
SG (2) SG10201610621XA (en)
WO (1) WO2013191651A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016072936A1 (en) * 2014-11-03 2016-05-12 Nanyang Technological University Novel recombinant expression systems
CN110951760B (en) * 2019-12-20 2021-09-03 台州职业技术学院 Protein time-delay expression switch and application thereof in production of glucaric acid

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7112721B2 (en) * 2000-02-08 2006-09-26 Sakata Seed Corporation Methods and constructs for plant transformation
NZ552628A (en) * 2004-07-15 2009-09-25 Dsm Ip Assets Bv Biochemical synthesis of 1,4-butanediamine
CA2628725A1 (en) * 2005-11-15 2007-05-31 Glycofi, Inc. Production of glycoproteins with reduced o-glycosylation
BR112014031526A2 (en) * 2012-06-19 2017-08-01 Dsm Ip Assets Bv promoters to express a gene in a cell
US9439972B2 (en) * 2012-09-10 2016-09-13 Ad Lunam Labs, Inc. Antifungal serum
US10077475B2 (en) * 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems

Also Published As

Publication number Publication date
EP2877582A4 (en) 2016-03-09
WO2013191651A1 (en) 2013-12-27
EP2877582A1 (en) 2015-06-03
US20150176039A1 (en) 2015-06-25
SG11201408353QA (en) 2015-01-29
EP2877582B1 (en) 2018-12-19
US10683521B2 (en) 2020-06-16

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