EA200601669A1 - Ячмень для получения напитка со стабильным вкусом - Google Patents
Ячмень для получения напитка со стабильным вкусомInfo
- Publication number
- EA200601669A1 EA200601669A1 EA200601669A EA200601669A EA200601669A1 EA 200601669 A1 EA200601669 A1 EA 200601669A1 EA 200601669 A EA200601669 A EA 200601669A EA 200601669 A EA200601669 A EA 200601669A EA 200601669 A1 EA200601669 A1 EA 200601669A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- barley
- nonenal
- trans
- drink
- getting
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C1/00—Preparation of malt
- C12C1/18—Preparation of malt extract or of special kinds of malt, e.g. caramel, black malt
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
- A01H1/101—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4624—Hordeum vulgarus [barley]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C11/00—Fermentation processes for beer
- C12C11/003—Fermentation of beerwort
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C12/00—Processes specially adapted for making special kinds of beer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C7/00—Preparation of wort
- C12C7/04—Preparation or treatment of the mash
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C7/00—Preparation of wort
- C12C7/04—Preparation or treatment of the mash
- C12C7/047—Preparation or treatment of the mash part of the mash being unmalted cereal mash
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Physiology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Non-Alcoholic Beverages (AREA)
- Cereal-Derived Products (AREA)
- Seasonings (AREA)
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
- Alcoholic Beverages (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Согласно данному изобретению обеспечен нуль-LOX-1-ячмень и продукты растительного происхождения, полученные из него, такие как солод, приготовленный с использованием зерен ячменя, дефектных в синтезе превращающего жирные кислоты фермента липоксигеназы-1. Указанный фермент является ответственным за основную активность, связанную с превращением линолевой кислоты в 9-гидропероксиоктадекадиеновую кислоту, метаболит липоксигеназного пути, которая посредством дополнительных ферментативных и спонтанных реакций может приводить к появлению транс-2-ноненаля. Данное изобретение позволяет пивоварам производить пиво, лишенное детектируемых транс-2-ноненаль-специфических привкусов, даже после продолжительного хранения этого напитка.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/800,200 US7420105B2 (en) | 2004-03-11 | 2004-03-11 | Barley for production of flavor-stable beverage |
PCT/DK2005/000160 WO2005087934A2 (en) | 2004-03-11 | 2005-03-09 | Barley for production of flavor-stable beverage |
Publications (2)
Publication Number | Publication Date |
---|---|
EA200601669A1 true EA200601669A1 (ru) | 2007-12-28 |
EA014705B1 EA014705B1 (ru) | 2011-02-28 |
Family
ID=34920665
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA200601669A EA014705B1 (ru) | 2004-03-11 | 2005-03-09 | Растение ячменя или его часть с мутантным геном липоксигеназы lox-1 и их применение |
Country Status (13)
Country | Link |
---|---|
US (5) | US7420105B2 (ru) |
EP (2) | EP1727905B1 (ru) |
JP (2) | JP4805249B2 (ru) |
CN (1) | CN1981041B (ru) |
AR (2) | AR049008A1 (ru) |
AU (1) | AU2005221763C1 (ru) |
BR (1) | BRPI0508554B1 (ru) |
CA (1) | CA2558815C (ru) |
EA (1) | EA014705B1 (ru) |
NZ (1) | NZ550204A (ru) |
UA (1) | UA89632C2 (ru) |
WO (1) | WO2005087934A2 (ru) |
ZA (1) | ZA200608444B (ru) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4113795B2 (ja) * | 2003-03-25 | 2008-07-09 | サッポロビール株式会社 | 大麦リポキシゲナーゼ−1遺伝子、大麦の選抜方法、麦芽アルコール飲料用原料及び麦芽アルコール飲料の製造方法 |
US7420105B2 (en) * | 2004-03-11 | 2008-09-02 | Carlsberg A/S | Barley for production of flavor-stable beverage |
CA2633592A1 (en) * | 2005-12-19 | 2007-06-28 | Suntory Limited | Method for processing sprouted cereal, malt product, fermented malt drink, and food and drink |
WO2007102850A1 (en) * | 2006-03-06 | 2007-09-13 | Lakefront Brewery, Inc. | Gluten-free beer and method for making the same |
US20100015306A1 (en) * | 2008-07-15 | 2010-01-21 | Pepsico, Inc. | Method for Preparing a Low Viscosity Whole Grain Flour Slurry |
US9587210B2 (en) * | 2008-12-03 | 2017-03-07 | Carlsberg Breweries A/S | Energy saving brewing method |
SG171940A1 (en) * | 2008-12-03 | 2011-07-28 | Carlsberg Breweries As | Barley and malt-derived beverages with low dimethyl sulfide level |
SA109310019B1 (ar) * | 2008-12-30 | 2014-09-10 | Carlsberg Breweries As | شعير له نشاط ليبوأوكسجيناز منخفض |
US20100178400A1 (en) * | 2009-01-13 | 2010-07-15 | Pepsico, Inc. | Method of Preparing a Whole Grain Beverage |
CA2801142C (en) * | 2010-06-03 | 2019-04-30 | Heineken Supply Chain B.V. | Energy saving brewing method |
CN103114129B (zh) * | 2012-11-08 | 2016-08-31 | 中国农业科学院作物科学研究所 | 一种大麦脂肪氧化酶(lox-1)合成缺陷基因的多态性分子标记方法 |
WO2014136568A1 (ja) * | 2013-03-05 | 2014-09-12 | サッポロビール株式会社 | 飲料及びこれに関する方法 |
CN106062168A (zh) * | 2014-01-02 | 2016-10-26 | 嘉士伯酿酒有限公司 | 风味稳定的饮料 |
RU2017130689A (ru) * | 2015-02-03 | 2019-03-04 | Фирмениш Са | Усовершенствованный способ получения ароматизаторов и душистых соединений |
CN104823973B (zh) * | 2015-05-22 | 2017-05-31 | 天津农学院 | 反式‑2‑壬烯醛在制备植物保鲜剂中的应用 |
EP3736321A1 (en) | 2016-07-01 | 2020-11-11 | Carlsberg Breweries A/S | Refined cereal-based beverages |
IL263451B (en) | 2016-07-01 | 2022-08-01 | Carlsberg As | A method to screen for a mutant within a population of organisms using a merge-and-split approach |
RU2672404C2 (ru) * | 2017-01-31 | 2018-11-14 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Орловский государственный аграрный университет имени Н.В. Парахина" (ФГБОУ ВО Орловский ГАУ) | Способ выделения гордецина из зерна ячменя |
CN108165501B (zh) * | 2017-11-24 | 2022-02-15 | 山东喜啤士生物科技有限公司 | 一株啤酒酿酒酵母菌及其培养方法与应用 |
CN111527192A (zh) | 2017-12-28 | 2020-08-11 | 嘉士伯有限公司 | 生产谷物提取物的方法以及将这种提取物加工成饮料的方法 |
CN111787788B (zh) | 2017-12-28 | 2024-03-19 | 嘉士伯有限公司 | 具有改善的细胞壁性质的谷类植物 |
EP4324902A3 (en) | 2017-12-28 | 2024-05-22 | Carlsberg A/S | Fast methods for preparing cereal extracts |
EA202091573A1 (ru) | 2017-12-28 | 2020-10-08 | Карлсберг А/С | Ячмень с повышенной активностью гидролитических ферментов |
JP2021524753A (ja) | 2018-04-25 | 2021-09-16 | カールスバーグ エー/エス | 大麦ベースの飲料 |
BE1026272B1 (nl) * | 2018-05-09 | 2019-12-11 | Anheuser Busch Inbev Sa | Een werkwijze voor het bereiden van een moutgebaseerde drank, een moutmengsel en het gebruik van voornoemd mengsel voor het bereiden van voornoemde moutgebaseerde drank |
IL292003A (en) * | 2019-10-10 | 2022-06-01 | Carlsberg As | A method for preparing mutant plants |
AR121488A1 (es) | 2020-03-02 | 2022-06-08 | Carlsberg As | Plantas de cebada con alta actividad de dextrinasa de límite |
US20230289492A1 (en) * | 2022-03-10 | 2023-09-14 | Aka Foods LTD | Mixture Modeling Systems and Methods |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5008294A (en) | 1985-02-11 | 1991-04-16 | Chemex Pharmaceuticals, Inc. | Methods of treating tumors with compositions of catecholic butanes |
US4708964A (en) | 1984-02-09 | 1987-11-24 | Chemex Pharmaceuticals | Lipoxygenase inhibitors |
US4880637A (en) | 1985-02-11 | 1989-11-14 | Chemex Pharmaceuticals, Inc. | Compositions of catecholic butanes with zinc |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US5231020A (en) * | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
US6274358B1 (en) * | 1994-03-25 | 2001-08-14 | Richard Barry Holtz | Method for providing green note compounds |
CN1131189A (zh) * | 1995-03-10 | 1996-09-18 | 孙新国 | 香啤酒及其生产方法 |
EP0859836A1 (en) * | 1995-10-13 | 1998-08-26 | Purdue Research Foundation | Improvement of fruit quality by inhibiting production of lipoxygenase in fruits |
US5844121A (en) * | 1996-01-19 | 1998-12-01 | The Texas A & M University System | Method of inhibiting mycotoxin production in seed crops by modifying lipoxygenase pathway genes |
DE69730551T2 (de) * | 1996-04-15 | 2005-09-01 | Givaudan S.A. | Hydroxyperoxide Lyasen |
SG72870A1 (en) * | 1997-10-23 | 2000-05-23 | Givaudan Roure Int | Process for the production of degradation products of fatty acids |
US7087813B2 (en) * | 1999-02-10 | 2006-08-08 | E.I. Du Pont De Nemours And Company | Plant lipoxygenases |
US6660915B2 (en) * | 2000-12-29 | 2003-12-09 | Anna Christina Douma | Low lipoxygenase 1 barley |
CZ298689B6 (cs) | 2000-12-29 | 2007-12-19 | Carlsberg Research Laboratory | Jecmen s nízkou aktivitou lipoxygenázy-1 |
WO2002053720A1 (en) | 2000-12-29 | 2002-07-11 | Carlsberg Research Laboratory | Low-lipoxygenase 1 barley |
US6842484B2 (en) | 2001-07-10 | 2005-01-11 | Motorola, Inc. | Method and apparatus for random forced intra-refresh in digital image and video coding |
JP4113795B2 (ja) * | 2003-03-25 | 2008-07-09 | サッポロビール株式会社 | 大麦リポキシゲナーゼ−1遺伝子、大麦の選抜方法、麦芽アルコール飲料用原料及び麦芽アルコール飲料の製造方法 |
US7420105B2 (en) * | 2004-03-11 | 2008-09-02 | Carlsberg A/S | Barley for production of flavor-stable beverage |
-
2004
- 2004-03-11 US US10/800,200 patent/US7420105B2/en active Active
-
2005
- 2005-03-09 CA CA2558815A patent/CA2558815C/en active Active
- 2005-03-09 EP EP05706820.7A patent/EP1727905B1/en active Active
- 2005-03-09 BR BRPI0508554-3A patent/BRPI0508554B1/pt active IP Right Grant
- 2005-03-09 UA UAA200610769A patent/UA89632C2/ru unknown
- 2005-03-09 EA EA200601669A patent/EA014705B1/ru unknown
- 2005-03-09 NZ NZ550204A patent/NZ550204A/en unknown
- 2005-03-09 WO PCT/DK2005/000160 patent/WO2005087934A2/en active Application Filing
- 2005-03-09 ZA ZA200608444A patent/ZA200608444B/xx unknown
- 2005-03-09 JP JP2007502192A patent/JP4805249B2/ja active Active
- 2005-03-09 AU AU2005221763A patent/AU2005221763C1/en active Active
- 2005-03-09 US US10/598,779 patent/US7838053B2/en active Active
- 2005-03-09 CN CN200580015328XA patent/CN1981041B/zh active Active
- 2005-03-09 EP EP10181800A patent/EP2290089A3/en not_active Withdrawn
- 2005-03-11 AR ARP050100930A patent/AR049008A1/es active IP Right Grant
-
2010
- 2010-11-22 US US12/951,569 patent/US20110195151A1/en not_active Abandoned
-
2011
- 2011-04-12 JP JP2011088647A patent/JP2011135900A/ja not_active Withdrawn
-
2012
- 2012-08-07 AR ARP120102888A patent/AR087480A2/es not_active Application Discontinuation
-
2014
- 2014-05-16 US US14/279,569 patent/US20150218498A1/en not_active Abandoned
-
2018
- 2018-09-28 US US16/145,998 patent/US20190078042A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1727905B1 (en) | 2022-12-28 |
JP2011135900A (ja) | 2011-07-14 |
NZ550204A (en) | 2008-11-28 |
CA2558815A1 (en) | 2005-09-22 |
US20110195151A1 (en) | 2011-08-11 |
AR087480A2 (es) | 2014-03-26 |
US20190078042A1 (en) | 2019-03-14 |
US20150218498A1 (en) | 2015-08-06 |
CN1981041B (zh) | 2011-03-23 |
ZA200608444B (en) | 2008-08-27 |
US20090029000A1 (en) | 2009-01-29 |
AU2005221763C1 (en) | 2013-12-05 |
AU2005221763A1 (en) | 2005-09-22 |
WO2005087934A3 (en) | 2006-02-02 |
AU2005221763B2 (en) | 2008-10-02 |
EP1727905A2 (en) | 2006-12-06 |
CN1981041A (zh) | 2007-06-13 |
BRPI0508554B1 (pt) | 2022-04-19 |
JP4805249B2 (ja) | 2011-11-02 |
UA89632C2 (ru) | 2010-02-25 |
US20050204437A1 (en) | 2005-09-15 |
US7838053B2 (en) | 2010-11-23 |
US7420105B2 (en) | 2008-09-02 |
CA2558815C (en) | 2014-01-07 |
EA014705B1 (ru) | 2011-02-28 |
EP2290089A3 (en) | 2011-08-31 |
EP2290089A2 (en) | 2011-03-02 |
AR049008A1 (es) | 2006-06-21 |
JP2007527721A (ja) | 2007-10-04 |
BRPI0508554A (pt) | 2007-08-07 |
WO2005087934A2 (en) | 2005-09-22 |
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