DK476686A - Fremgangsmaade til udfoerelse af stedsspecifik rekombination af dna i gaer - Google Patents

Fremgangsmaade til udfoerelse af stedsspecifik rekombination af dna i gaer Download PDF

Info

Publication number
DK476686A
DK476686A DK476686A DK476686A DK476686A DK 476686 A DK476686 A DK 476686A DK 476686 A DK476686 A DK 476686A DK 476686 A DK476686 A DK 476686A DK 476686 A DK476686 A DK 476686A
Authority
DK
Denmark
Prior art keywords
yeast
dna
specific recombination
procedure
performing location
Prior art date
Application number
DK476686A
Other languages
Danish (da)
English (en)
Other versions
DK476686D0 (da
Inventor
Brian Lee Sauer
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of DK476686D0 publication Critical patent/DK476686D0/da
Publication of DK476686A publication Critical patent/DK476686A/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK476686A 1985-10-07 1986-10-06 Fremgangsmaade til udfoerelse af stedsspecifik rekombination af dna i gaer DK476686A (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US78495185A 1985-10-07 1985-10-07

Publications (2)

Publication Number Publication Date
DK476686D0 DK476686D0 (da) 1986-10-06
DK476686A true DK476686A (da) 1987-04-08

Family

ID=25134033

Family Applications (1)

Application Number Title Priority Date Filing Date
DK476686A DK476686A (da) 1985-10-07 1986-10-06 Fremgangsmaade til udfoerelse af stedsspecifik rekombination af dna i gaer

Country Status (9)

Country Link
US (1) US4959317A (de)
EP (1) EP0220009B1 (de)
JP (1) JP2721666B2 (de)
AT (1) ATE85649T1 (de)
CA (1) CA1293460C (de)
DE (1) DE3687734T2 (de)
DK (1) DK476686A (de)
ES (1) ES2053438T3 (de)
IE (1) IE60421B1 (de)

Families Citing this family (271)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT88556B (pt) * 1987-09-28 1992-11-30 Smithkline Biolog Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras
WO1990005144A1 (en) 1988-11-11 1990-05-17 Medical Research Council Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors
US6291159B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6969586B1 (en) 1989-05-16 2005-11-29 Scripps Research Institute Method for tapping the immunological repertoire
US6680192B1 (en) 1989-05-16 2004-01-20 Scripps Research Institute Method for producing polymers having a preselected activity
US6291161B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
US6291158B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
GB8920211D0 (en) * 1989-09-07 1989-10-18 Ici Plc Diagnostic method
WO1991009957A1 (en) * 1989-12-22 1991-07-11 E.I. Du Pont De Nemours And Company Site-specific recombination of dna in plant cells
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
KR930701625A (ko) * 1990-07-13 1993-06-12 리처드 에프. 셀든 라이브러리 스크리닝 방법
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
WO1992015694A1 (en) * 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
WO1992022650A1 (en) * 1991-06-17 1992-12-23 Life Technologies, Inc. Recombination-facilitated multiplex analysis of dna fragments
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
WO1993015191A1 (en) * 1992-01-24 1993-08-05 Life Technologies, Inc. Modulation of enzyme activities in the in vivo cloning of dna
AU4401993A (en) * 1992-06-04 1993-12-30 Exemplar Corporation Insertion of heterologous dna outside of known chromosomal genes
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5733733A (en) * 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5614389A (en) * 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
GB9302798D0 (en) * 1993-02-12 1993-03-31 Medical Res Council Improvements in or relating to cloning dna
US5843744A (en) * 1993-07-08 1998-12-01 Ecogen Inc. Bacillus thuringiensis Tn5401 proteins
US5441884A (en) * 1993-07-08 1995-08-15 Ecogen Inc. Bacillus thuringiensis transposon TN5401
US5731172A (en) * 1994-03-09 1998-03-24 Sumitomo Pharmaceuticals Company, Ltd. Recombinant adenovirus and process for producing the same
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
EG23907A (en) * 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
JPH10507624A (ja) * 1994-08-18 1998-07-28 アリアド・ジーン・セラピューティクス・インコーポレーテッド 遺伝子発現、遺伝子産物機能および工学処理された宿主細胞の調節可能な除去
JP4216350B2 (ja) * 1994-09-19 2009-01-28 大日本住友製薬株式会社 動物細胞感染用の組換えｄｎａウイルスベクター
JP3770333B2 (ja) * 1995-03-15 2006-04-26 大日本住友製薬株式会社 組換えｄｎａウイルスおよびその製造方法
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US6091001A (en) * 1995-03-29 2000-07-18 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6974694B2 (en) * 1995-06-07 2005-12-13 Advec, Inc. Adenoviruses for control of gene expression
US5629159A (en) * 1995-06-07 1997-05-13 California Institute Of Technology Immortalization and disimmortalization of cells
EP0937098B1 (de) 1995-06-07 2002-08-14 Invitrogen Corporation Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US20060110798A1 (en) * 1995-06-07 2006-05-25 Graham Frank L Cells expressing recombinase useful for adenovirus vector production and control of gene expression
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US5801030A (en) * 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US6406847B1 (en) * 1995-10-02 2002-06-18 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
US7153652B2 (en) * 1995-10-02 2006-12-26 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
CA2237392A1 (en) 1995-11-13 1997-05-22 University Of Rochester Production of somatic mosaicism in mammals using a recombinatorial substrate
EP0927263A1 (de) * 1996-01-05 1999-07-07 Genetic Therapy, Inc. Rekombinase-vermittelte herstellung adenoviraler vektoren
US6087129A (en) * 1996-01-19 2000-07-11 Betagene, Inc. Recombinant expression of proteins from secretory cell lines
WO1997032481A1 (en) 1996-03-07 1997-09-12 The Regents Of The University Of California Helper-free, totally defective adenovirus for gene therapy
US6132989A (en) * 1996-06-03 2000-10-17 University Of Washington Methods and compositions for enhanced stability of non-adenoviral DNA
US5928914A (en) * 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US6534314B1 (en) 1996-06-14 2003-03-18 Massachusetts Institute Of Technology Methods and compositions for transforming cells
US6025192A (en) * 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
US6255071B1 (en) 1996-09-20 2001-07-03 Cold Spring Harbor Laboratory Mammalian viral vectors and their uses
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6066778A (en) * 1996-11-06 2000-05-23 The Regents Of The University Of Michigan Transgenic mice expressing APC resistant factor V
US5994620A (en) * 1996-12-10 1999-11-30 The Jackson Laboratory Induced chromosomal deletion
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6787319B2 (en) * 1997-04-16 2004-09-07 American Home Products Corp. β-amyloid peptide-binding proteins and polynucleotides encoding the same
US6774279B2 (en) * 1997-05-30 2004-08-10 Carnegie Institution Of Washington Use of FLP recombinase in mice
US6284944B1 (en) * 1997-08-29 2001-09-04 Cephalon, Inc, Gene-targeted non-human mammal with a human fad presenilin mutation and generational offspring
US20090074718A1 (en) * 1997-10-16 2009-03-19 Ivarie Robert D Avian derived erythropoietin
US8563803B2 (en) * 1997-10-16 2013-10-22 Synageva Biopharma Corp. Method of making protein in an egg of a transgenic chicken
US20040019923A1 (en) * 1997-10-16 2004-01-29 Ivarie Robert D. Exogenous proteins expressed in avians and their eggs
US7129390B2 (en) * 1997-10-16 2006-10-31 Avigenics, Inc Poultry Derived Glycosylated Interferon Alpha 2b
ES2357495T3 (es) * 1997-10-16 2011-04-27 University Of Georgia Research Foundation, Inc. Aves transgénicas y producción de proteína.
US7511120B2 (en) * 1997-10-16 2009-03-31 Synageva Biopharma Corp. Glycosylated G-CSF obtained from a transgenic chicken
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
WO1999021977A1 (en) 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
AU757930B2 (en) * 1997-12-01 2003-03-13 Roche Diagnostics Gmbh Optimization of cells for endogenous gene activation
US6808921B1 (en) 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
WO2000005355A1 (en) * 1998-07-24 2000-02-03 Baylor College Of Medicine Rapid subcloning using site-specific recombination
TWI255853B (en) 1998-08-21 2006-06-01 Kirin Brewery Method for modifying chromosomes
US20040009535A1 (en) 1998-11-27 2004-01-15 Celltech R&D, Inc. Compositions and methods for increasing bone mineralization
EP1133558B2 (de) 1998-11-27 2016-04-13 UCB Pharma S.A. Zusammensetzungen und verfahren zur erhöhung der knochenmineralisierung
ATE443074T1 (de) 1999-03-02 2009-10-15 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
JP2002537800A (ja) * 1999-03-02 2002-11-12 インビトロゲン・コーポレーション 毒性遺伝子に対して耐性のある細胞およびその使用
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
EP1194540A1 (de) * 1999-07-14 2002-04-10 Clontech Laboratories Inc. Rekombinase-basierende methoden zur herstellung von expressionsvektoren und zusammensetzungen für ihre verwendung
DE60043985D1 (de) * 1999-07-14 2010-04-22 Transgenic Inc "gen-fallen-vektor" und verfahren zum "einfangen" von genen mittels dieses vektors
EP2278022A3 (de) 1999-11-01 2011-05-18 Novartis Vaccines and Diagnostics, Inc. Expressionsvektoren, Transfektionssysteme und Verfahren zu deren Verwendung
WO2001035735A1 (en) * 1999-11-19 2001-05-25 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
US7414170B2 (en) * 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
US7253334B2 (en) 2000-12-22 2007-08-07 Aurox, Llc Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells
US7074983B2 (en) * 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
CA2392753A1 (en) 1999-12-10 2001-06-14 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
AU1647501A (en) 1999-12-10 2001-06-18 Cytos Biotechnology Ag Replicon based activation of endogenous genes
DE10004996C2 (de) * 2000-02-04 2002-09-26 Infineon Technologies Ag Vorrichtung und Verfahren zur Selbstkalibrierung von Faltungs-Analog/Digitalwandlern
WO2001062892A2 (en) * 2000-02-25 2001-08-30 Stratagene Method for ligating nucleic acids and molecular cloning
US7517688B2 (en) * 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
US6580019B1 (en) 2000-03-09 2003-06-17 Dekalb Genetics Corporation Non-reciprocal recombination-mediated transgene deletion in transgenic plants
US6750379B2 (en) * 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
WO2001068131A1 (en) 2000-03-13 2001-09-20 Cornell Research Foundation, Inc. Blocking leukocyte emigration and inflammation by interfering with cd99/hec2
US20040231006A1 (en) * 2000-04-12 2004-11-18 Silver Daniel P. Self-extinguishing recombinases, nucleic acids encoding them and methods of using the same
US6852530B2 (en) 2000-04-12 2005-02-08 Dana Farber Cancer Institute, Inc. Self-extinguishing recombinases, nucleic acids encoding them and methods of using the same
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AU3942202A (en) 2000-11-30 2002-06-11 Medarex Inc Transgenic transchromosomal rodents for making human antibodies
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
EP1860199B1 (de) 2001-01-12 2014-03-05 Exelixis, Inc. Modulierte Signalisierung von Insulinrezeptoren
US7126039B2 (en) * 2001-03-21 2006-10-24 Geron Corporation Animal tissue with carbohydrate antigens compatible for human transplantation
EP1370132A2 (de) * 2001-03-21 2003-12-17 Geron Corporation Verwendung von telomerase reverse-transkriptase zur herstellung von homozygote "knock-out" tieren
WO2002081711A1 (en) * 2001-04-06 2002-10-17 Cropdesign N.V. The use of double and opposite recombination sites for the single step cloning of two dna segments
EP1390394A4 (de) * 2001-04-19 2004-05-26 Invitrogen Corp Zusammensetzungen und verfahren zur rekombinationsklonierung von nukleinsäuremolekülen
US20020188965A1 (en) 2001-04-20 2002-12-12 Zou-Yu Zhao Methods of transforming plants
EP1632571B1 (de) * 2001-05-11 2012-11-28 Kyowa Hakko Kirin Co., Ltd. Künstliches menschliches Chromosom mit dem Gen für die menschliche Antikörper Lambda leichte Kette und ein nicht-menschliches Tier enthaltend ein künstliches menschliches Chromosom fähig zur genetischen Transmission
MXPA03010626A (es) * 2001-05-30 2004-12-06 Chromos Molecular Systems Inc Plataformas a base de cromosomas.
US20030092183A1 (en) * 2001-09-21 2003-05-15 Fisher Katherine E. Rapid creation of gene targeting vectors using homologous recombination in yeast
US20040023910A1 (en) * 2001-09-28 2004-02-05 Zhiming Zhang Use of cyr61 in the treatment and diagnosis of human uterine leiomyomas
EP1298207B2 (de) 2001-10-01 2015-12-30 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Verfahren zur Herstellung von Protein-Bibliotheken und zur Selektion von Proteinen daraus
DE10211063A1 (de) 2002-03-13 2003-10-09 Axaron Bioscience Ag Neue Verfahren zur Detektion und Analyse von Protein-Interaktionen in vivo
US8304233B2 (en) 2002-06-04 2012-11-06 Poetic Genetics, Llc Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
AU2003254184A1 (en) * 2002-07-26 2004-02-16 Genecopoeia, Inc. Methods and nucleic acid vectors for rapid expression and screening of cdna clones
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US20040229230A1 (en) * 2002-08-27 2004-11-18 Sleckman Barry P. Method for gene isolation by Cre-trap cloning
US7285699B2 (en) * 2002-10-07 2007-10-23 Stowers Institute For Medical Research Ends-out gene targeting method
WO2004042007A2 (en) * 2002-10-30 2004-05-21 University Of Iowa Research Foundation Somatic cell gene targeting vectors and methods of use thereof
US20030175968A1 (en) * 2002-10-30 2003-09-18 Golic Kent G. Gene targeting method
US7429690B2 (en) * 2002-11-08 2008-09-30 Kirin Holdings Kabushiki Kaisha Transgenic bovines having reduced prion protein production
US7078234B2 (en) 2002-12-18 2006-07-18 Monsanto Technology Llc Maize embryo-specific promoter compositions and methods for use thereof
US20060275766A1 (en) * 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
BRPI0406678A (pt) * 2003-01-07 2005-12-20 Symphogen As Método para produzir proteìnas policlonais recombinantes
US20040133944A1 (en) * 2003-01-08 2004-07-08 Delta And Pine Land Company Seed oil suppression to enhance yield of commercially important macromolecules
US7803362B2 (en) * 2003-01-24 2010-09-28 Synageva Biopharma Corp. Glycosylated interferon alpha
US7271313B2 (en) * 2003-04-09 2007-09-18 The Brigham And Women's Hospital, Inc. Presenilin-deficient mouse model of age-dependent neurodegeneration and cognitive loss
EP1881074B1 (de) 2003-05-05 2013-01-02 Monsanto Technology, LLC HRGP Promotorkonstrukte
WO2005027625A2 (en) * 2003-09-18 2005-03-31 Mayo Foundation For Medical Education And Research Transgenic tieg non-human animals
RU2006114432A (ru) * 2003-09-30 2007-11-20 Лексикон Дженетикс Инкорпорейтед (Us) Способы и композиции для определения функции гена
DE602004029089D1 (de) 2003-10-02 2010-10-21 Monsanto Technology Llc Stapeln von merkmalen zur verbesserung von nutzpflanzen in transgenen pflanzen
EP1697534B1 (de) 2003-12-01 2010-06-02 Life Technologies Corporation Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US20050235370A1 (en) * 2003-12-12 2005-10-20 National Health Research Institutes Spatiotemporally controlled adult somatic mutagenesis system
WO2005063007A1 (en) 2003-12-30 2005-07-14 Ullevål Universitetssykehus Hf Non-human mammal comprising a modified serca2 gene and methods, cells, genes, and vectors thereof
US7683237B2 (en) 2004-02-10 2010-03-23 Monsanto Technology Llc Maize seed with synergistically enhanced lysine content
AR047598A1 (es) 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
US20060041961A1 (en) 2004-03-25 2006-02-23 Abad Mark S Genes and uses for pant improvement
PT1732379E (pt) 2004-04-09 2014-01-03 Monsanto Technology Llc Composições e métodos para o controlo de infestações de plantas por insectos
US7420099B2 (en) * 2004-04-22 2008-09-02 Kirin Holdings Kabushiki Kaisha Transgenic animals and uses thereof
DE602005016818D1 (de) 2004-07-20 2009-11-05 Symphogen As Verfahren zur charakterisierung einer polyklonalen zellinie
US7582741B2 (en) 2004-07-26 2009-09-01 University Of Massachusetts Conditional disruption of dicer1 in cell lines and non-human mammals
US20090035784A1 (en) * 2004-07-30 2009-02-05 Mount Sinai School Of Medicine Of New York University Npc1l1 and npc1l1 inhibitors and methods of use thereof
US20060075522A1 (en) 2004-07-31 2006-04-06 Jaclyn Cleveland Genes and uses for plant improvement
CN101084317A (zh) * 2004-10-22 2007-12-05 人类多克隆治疗公司 对内源免疫球蛋白表达的抑制
US7501275B2 (en) * 2004-10-27 2009-03-10 The United States Of America As Represented By The Secretary Of Agriculture Yeast transformation system
EP1817591A2 (de) * 2004-11-23 2007-08-15 The Government of the United States of America, as represented by the Secretary of Health and Human Services Verfahren und zusammensetzungen zur ex-vivo-hochdurchsatzdetektion von protein-protein-wechselwirkungen
US20080244765A1 (en) * 2004-12-16 2008-10-02 Pioneer Hi-Bred International, Inc. Methods and compositions for pollination disruption
US8895818B2 (en) 2004-12-21 2014-11-25 Monsanto Technology Llc Transgenic plants with enhanced agronomic traits
US20070294782A1 (en) 2004-12-21 2007-12-20 Mark Abad Transgenic plants with enhanced agronomic traits
US20060154285A1 (en) * 2004-12-29 2006-07-13 Robishaw Janet D Zebrafish heterotrimer G-protein gamma 2 subunit (GNG2)
WO2006074400A2 (en) 2005-01-07 2006-07-13 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Method to trigger rna interference
WO2006076423A2 (en) 2005-01-12 2006-07-20 Monsanto Technology, Llc Genes and uses for plant improvement
US20060174362A1 (en) 2005-02-01 2006-08-03 Origen Therapeutics, Inc. Long-term culture of avian primordial germ cells (PGCs)
US8519225B2 (en) 2006-02-10 2013-08-27 Monsanto Technology Llc Identification and use of target genes for control of plant parasitic nematodes
WO2006138005A2 (en) 2005-05-10 2006-12-28 Monsanto Technology, Llc Genes and uses for plant improvement
CN101437539B (zh) * 2005-07-05 2013-10-02 康奈尔研究基金会（有限公司） 通过干扰cd99l2阻断白细胞迁出和炎症
CN101611142A (zh) * 2005-07-07 2009-12-23 综合医院公司 用于控制干细胞分化的方法
US9121028B2 (en) * 2005-09-09 2015-09-01 Monsanto Technology Llc Selective gene expression in plants
PT2431473T (pt) 2005-09-16 2017-02-15 Monsanto Technology Llc Métodos para o controlo genético de infestações por insetos em plantas e suas composições
PT2275562E (pt) 2005-09-16 2016-01-26 Devgen Nv Métodos com base em plantas transgénicas para infestações em plantas utilizando arn de interferência¿
US20070079396A1 (en) 2005-10-03 2007-04-05 Malvar Thomas M Transgenic plant seed with increased lysine
JP2009524406A (ja) * 2005-10-13 2009-07-02 ビーシー キャンサー エージェンシー 合成生物学および代謝工学のためのモジュラー型ゲノム
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
EP1962577A4 (de) 2005-12-21 2009-12-16 Monsanto Technology Llc Transgene pflanzen mit verbesserten agronomischen merkmalen
CN105385679B (zh) 2006-02-13 2020-05-26 孟山都技术有限公司 选择和稳定dsRNA构建体
JP5301424B2 (ja) * 2006-03-15 2013-09-25 ブライアン アール． ソーパー， 細胞における表現型および遺伝型の変動のスクリーニングおよびマッピングの方法
US7812127B2 (en) 2006-03-17 2010-10-12 Synageva Biopharma Corp. Glycosylated human G-CSF
US20070250941A1 (en) * 2006-03-27 2007-10-25 Genentech, Inc. VEGF Receptor Conditional Knockout Animals and Methods of Use
US7919322B2 (en) * 2006-03-29 2011-04-05 Sigma-Aldrich Co. Targeted deletions using retroelement-mediated placement of recombination sites
JP5524610B2 (ja) 2006-04-07 2014-06-18 ザ リサーチ ファウンデーション オブ ステイト ユニバーシティー オブ ニューヨーク トランスコバラミン受容体ポリペプチド、核酸およびその調節因子、ならびに細胞の増殖の調節ならびにガンおよびコバラミン欠損症の処置に使用する関連方法
AU2007249385B2 (en) 2006-05-09 2012-09-13 The Curators Of The University Of Missouri Plant artificial chromosome platforms via telomere truncation
EP2540831A3 (de) 2006-08-17 2013-04-10 Monsanto Technology, LLC Transgene Pflanzen mit erweiterten agronomischen Merkmalen
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US20090105462A1 (en) * 2006-11-09 2009-04-23 Ivarie Robert D Glycosylated erythropoietin
WO2008091680A2 (en) * 2007-01-25 2008-07-31 The General Hospital Corporation Methods for controlling stem cell differentiation
KR101471445B1 (ko) * 2007-01-26 2014-12-15 시나게바 바이오파르마, 코포레이션 조류에서 이식유전자 발현
EP2113029A4 (de) * 2007-02-09 2010-06-02 Univ Utah Res Found In vivo genomweite mutagenese
WO2008103475A1 (en) * 2007-02-20 2008-08-28 Anaptysbio, Inc. Somatic hypermutation systems
WO2008112542A1 (en) * 2007-03-09 2008-09-18 The Curators Of The University Of Missouri Methods for conditional and inducible transgene expression to direct the development of stem cells
DE102007041655A1 (de) 2007-09-03 2009-03-05 Medicyte Gmbh Vermehrung von primären Zellen und deren Verwendung
CA2697193C (en) 2007-09-14 2017-06-06 Adimab, Inc. Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
ES2399088T3 (es) 2007-11-01 2013-03-25 Perseid Therapeutics Llc Polipéptidos y ácidos nucleicos inmunosupresores
FR2927089B1 (fr) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
US20090233809A1 (en) * 2008-03-04 2009-09-17 Affymetrix, Inc. Resequencing methods for identification of sequence variants
US8093043B2 (en) * 2008-06-04 2012-01-10 New York University β-TrCP1, β-TrCP2 and RSK1 or RSK2 inhibitors and methods for sensitizing target cells to apoptosis
CA2729626C (fr) * 2008-07-11 2017-12-12 Jean-Marc Nicaud Nouvelles souches de levure mutantes capables d'accumuler une grande quantite de lipides
US20110262485A1 (en) 2008-08-04 2011-10-27 University Of Miami Sting (stimulator of interferon genes), a regulator of innate immune responses
WO2010033854A2 (en) 2008-09-19 2010-03-25 Synageva Biopharma Corp. Avian derived fusion proteins
WO2010042490A1 (en) * 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
US20110258735A1 (en) 2008-12-22 2011-10-20 Marie Coffin Genes and uses for plant enhancement
EP2206723A1 (de) 2009-01-12 2010-07-14 Bonas, Ulla Modulare DNA-bindende Domänen
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
EP2414507B1 (de) 2009-04-03 2014-07-02 Medical Research Council Mutanten von aktivierungsinduzierter cytidin-deaminase (aid) und verwendungsverfahren
EP3260545B1 (de) 2009-04-20 2020-07-01 Monsanto Technology LLC Resistenz gegen mehrere viren in pflanzen
US9314005B2 (en) 2009-07-01 2016-04-19 Transposagen Biopharmaceuticals, Inc. Genetically modified rat models for severe combined immunodeficiency (SCID)
WO2011011678A2 (en) 2009-07-24 2011-01-27 Transposagen Biopharmaceuticals, Inc. Genetically modified rat models for cytokine-cytokine signaling pathways
US20110145936A1 (en) * 2009-07-30 2011-06-16 Ostertag Eric M Genetically Modified Rat Models for Pharmacokinetics
US20110035816A1 (en) * 2009-08-05 2011-02-10 Ostertag Eric M Genetically Modified Rat Models for Drug Metabolism
US20120151609A1 (en) 2009-08-20 2012-06-14 Ostertag Eric M Genetically Modified Rat Models for Pain
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
AU2010327998B2 (en) 2009-12-10 2015-11-12 Iowa State University Research Foundation, Inc. TAL effector-mediated DNA modification
WO2011146121A1 (en) 2010-05-17 2011-11-24 Sangamo Biosciences, Inc. Novel dna-binding proteins and uses thereof
DK2597945T3 (da) 2010-07-26 2020-09-21 Trianni Inc Transgene dyr og fremgangsmåder til anvendelse deraf
DE102010041958A1 (de) 2010-10-04 2012-04-05 Medicyte Gmbh Geeignete Hepatozyten für in-vitro Genotoxitätstests
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
AU2011329872B2 (en) 2010-11-16 2017-04-13 Excelimmune, Inc. Methods for producing recombinant proteins
WO2012129198A1 (en) 2011-03-23 2012-09-27 Transposagen Biopharmaceuticals, Inc. Genetically modified rat models for obesity and diabetes
US9441255B2 (en) 2011-07-21 2016-09-13 The Regents Of The University Of California Transcription factors for cellulosic enzyme production
WO2013032850A2 (en) 2011-08-26 2013-03-07 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US9598474B2 (en) 2012-03-26 2017-03-21 Axcella Health, Inc. Nutritive fragments, proteins and methods
EP4001427A1 (de) 2012-04-24 2022-05-25 Gen9, Inc. Verfahren zum sortieren von nukleinsäuren und zur multiplexierten, vorbereitenden in-vitro-klonung
CA2877290A1 (en) 2012-06-19 2013-12-27 Daniel F. Voytas Gene targeting in plants using dna viruses
CN113512577A (zh) 2012-06-25 2021-10-19 Gen9股份有限公司 用于核酸组装和高通量测序的方法
EP2690177B1 (de) 2012-07-24 2014-12-03 Technische Universität Dresden Protein mit Rekombinase-Aktivität zur ortspezifischen DNA-Rekombination
NZ707560A (en) 2012-11-01 2019-10-25 Cellectis Plants for production of therapeutic proteins
CA2895909C (en) 2012-12-21 2021-07-06 Cellectis Potatoes with reduced cold-induced sweetening
EP3919505B1 (de) 2013-01-16 2023-08-30 Emory University Verwendungen von cas9-nukleinsäurekomplexen
US10113162B2 (en) 2013-03-15 2018-10-30 Cellectis Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes
EP2810952A1 (de) 2013-06-03 2014-12-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Neuartige Schädlingsbekämpfungsverfahren
EP2927685A1 (de) 2014-04-02 2015-10-07 Medicyte GmbH Geeignete Hepatozyten für In-vitro-Hepatitistests
WO2015193858A1 (en) 2014-06-20 2015-12-23 Cellectis Potatoes with reduced granule-bound starch synthase
CN106460022B (zh) 2014-06-24 2018-06-05 加州大学董事会 木糖甙木糖醇寡聚物的生产方法
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
US20180105832A1 (en) 2015-04-13 2018-04-19 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. Novel aflatoxin and fungal infection control methods
CN106191041B (zh) * 2015-04-30 2020-12-29 杭州菁因康生物科技有限公司 基因打靶方法
WO2016191734A1 (en) 2015-05-28 2016-12-01 University Of California Berkeley Monofunctional aldehyde and alcohol dehydrogenases for production of fuels and commodity chemicals
WO2016210373A2 (en) 2015-06-24 2016-12-29 Synlogic, Inc. Recombinant bacteria engineered for biosafety, pharmaceutical compositions, and methods of use thereof
US10837024B2 (en) 2015-09-17 2020-11-17 Cellectis Modifying messenger RNA stability in plant transformations
WO2017128039A1 (zh) * 2016-01-26 2017-08-03 浙江大学 基因组合及其用途
EP3410843A1 (de) 2016-02-02 2018-12-12 Cellectis Modifizierung von sojaölzusammensetzung durch gezieltes knockout der fad3a/1b-gene
BR112019002238A2 (pt) 2016-08-05 2019-05-14 Cargill, Incorporated polipeptídeo e célula manipulados, e, método de fermentação.
US11674151B2 (en) 2016-08-26 2023-06-13 Board Of Trustees Of Michigan State University Transcription factors to improve resistance to environmental stress in plants
UY37482A (es) 2016-11-16 2018-05-31 Cellectis Métodos para alterar el contenido de aminoácidos en plantas mediante mutaciones de desplazamiento de marco
AU2018260469A1 (en) 2017-04-25 2019-11-14 Cellectis Alfalfa with reduced lignin composition
US11046969B2 (en) 2017-05-24 2021-06-29 Epiplanta Biotech Ltd. Transgenic plant and the method for producing the same
WO2018228961A1 (en) 2017-06-12 2018-12-20 Johann Wolfgang Goethe-Universität Frankfurt am Main Genetic tools and procedure for the phenotypic identification of the genotype of transgenic diploid organisms
CN110914414A (zh) 2017-06-14 2020-03-24 德累斯顿工业大学 利用设计dna重组酶遗传改变基因组的方法与手段
HRP20220615T1 (hr) 2017-06-30 2022-06-24 Inscripta, Inc. Postupci, moduli, instrumenti i sustavi za automatiziranu obradu stanica
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
US10443074B2 (en) 2017-09-30 2019-10-15 Inscripta, Inc. Modification of cells by introduction of exogenous material
CA3087266A1 (en) 2018-01-09 2019-07-18 Cibus Us Llc Shatterproof genes and mutations
EP3775159A4 (de) 2018-03-29 2022-01-19 Inscripta, Inc. Automatisierte steuerung von zellwachstumsgeschwindigkeiten für induktion und transformation
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
IL292273B2 (en) 2018-08-14 2023-10-01 Inscripta Inc Devices, modules and methods for improved detection of edited sequences in living cells
CA3108892A1 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
CN114008070A (zh) 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
CA3144958A1 (en) 2019-07-01 2021-01-07 Trianni, Inc. Transgenic mammals and methods of use thereof
JP2022538886A (ja) 2019-07-01 2022-09-06 トリアニ・インコーポレイテッド トランスジェニック哺乳動物およびその使用法
JP2022539248A (ja) 2019-07-02 2022-09-07 フレッド ハッチンソン キャンサー リサーチ センター 組換えａｄ３５ベクター及び関連遺伝子治療改善
US11939588B2 (en) 2019-10-17 2024-03-26 Board Of Trustees Of Michigan State University Elevated resistance to insects and plant pathogens without compromising seed production
EP3825408A1 (de) 2019-11-19 2021-05-26 FRAUNHOFER-GESELLSCHAFT zur Förderung der angewandten Forschung e.V. Verfahren zur insektenschädlingsbekämpfung für mehrere arten
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CN116997651A (zh) 2021-01-19 2023-11-03 J·大卫格莱斯顿研究所-根据J·大卫格莱斯顿遗嘱的遗嘱信托 用于增强人t细胞功能的基因激活靶标
AU2022268937A1 (en) 2021-05-05 2023-10-26 Trianni, Inc. Transgenic rodents expressing chimeric equine-rodent antibodies and methods of use thereof
CA3235395A1 (en) 2021-11-10 2023-05-19 Peter Burrows Transgenic mammals and methods of use thereof
WO2023150393A2 (en) 2022-02-07 2023-08-10 Ensoma, Inc. Inhibitor-resistant mgmt modifications and modification of mgmt-encoding nucleic acids
WO2023156906A1 (en) 2022-02-15 2023-08-24 Futuragene Israel Ltd A bacillus thuringiensis pesticidal protein (bt pp) combination useful for plant protection
WO2024097418A2 (en) 2022-11-03 2024-05-10 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Gene activation and interference targets for exhaustion/dysfunction-resistant t cell products and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4626505A (en) * 1984-02-24 1986-12-02 E. I. Du Pont De Nemours And Company Selectable markers for yeast transformation
US4673640A (en) * 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination

Also Published As

Publication number Publication date
EP0220009A2 (de) 1987-04-29
JP2721666B2 (ja) 1998-03-04
EP0220009A3 (en) 1988-09-21
ATE85649T1 (de) 1993-02-15
IE60421B1 (en) 1994-07-13
CA1293460C (en) 1991-12-24
DE3687734T2 (de) 1993-05-27
ES2053438T3 (es) 1994-08-01
JPS62175184A (ja) 1987-07-31
DE3687734D1 (de) 1993-03-25
IE862631L (en) 1987-04-07
DK476686D0 (da) 1986-10-06
EP0220009B1 (de) 1993-02-10
US4959317A (en) 1990-09-25

Similar Documents

Publication Publication Date Title
DK476686A (da) Fremgangsmaade til udfoerelse af stedsspecifik rekombination af dna i gaer
ATE37198T1 (de) Vektor-plasmide.
DK496485A (da) Transformation af gaer-stammer af slaegten pichia
MX165920B (es) Un procedimiento para producir glucoamilasa
CA2071943A1 (en) Site-specific recombination of dna in plant cells
DE68925083D1 (de) Rekombinante DNS, diese rekombinante DNS enthaltender Mikroorganismus und Verfahren zur Herstellung von L-Aminosäure unter Verwendung dieses Mikroorganismus
NO864275L (no) Fremgangsmaate til seteselektiv genomisk modifisering av gjaer av slekten pichia.
DK0909815T3 (da) DNA-sekvens, som bibringer en cytoplasmatisk hansterilitet, mitochondriegenom, cellekernegenom, mitochondrie og plante, som indeholder denne sekvens, og fremgangsmåde til fremstilling af hybrider
DE69637631D1 (de) Künstliche säuger chromosome
DE3583194D1 (de) Verfahren zur herstellung von einem polypeptid mittels eines transformierten mikroorganismus, ein dazu geeigneter transformierter mikroorganismus, und zur herstellung dieser mikroorganismen geeignete dns sequenzen.
DE69417521T2 (de) Elektrode einer zelle, sekundärzelle mit dieser elektrode und verfahren zur herstellung dieser elektrode
IL87170A0 (en) Stable and viable recombinant animal cell viral vectors and their preparation
DK176285D0 (da) Fremstilling af polypeptider med human-gamma-interferonaktivitet
NO175003C (no) Fusjonsproteiner med eukaryotisk ballastandel, vektor som inneholder et DNA som koder for fusjonsproteinet samt E.coli celle inneholdene vektoren
NO931357L (no) Segmentert kopolymer, fremgangsmåte til fremstilling derav, samt blanding der derav med polypropylen
DE3587205D1 (de) Verfahren zur herstellung eines proteins und dafuer zu verwendender vektor, rekombinante dns und transformierte zelle.
NO175872C (no) Plasmidvektor, fremgangsmåte for fremstilling av peptider og myelomcelle
FR2588551B1 (fr) Procede de fabrication d'une composition propulsive double-base a faible emission de lueur de flamme, et composition propulsive ainsi obtenue
DK391185D0 (da) Rekombinant saccharomyces
PT80483B (pt) Processo para a preparacao de polipeptidos a partir de aminoacidos geneticamente codificavel
DE3575163D1 (de) Verfahren zur kultivierung transformierter hefe.
MX9308031A (es) Procedimiento para la produccion de polipeptidos.
DE3571647D1 (en) A positive retroregulatory element, plasmid and cells comprising said element and their production, a method of enhancing gene expression using said element and a method of enhancing rna half life using said element
DK138984A (da) Plasmid, fremgangsmaade til fremstilling heraf, mikroorganismer indeholdende samme samt fremgangsmaade til dyrkning af mikroorganismen
WO1993018151A1 (fr) Element transposable originaire d'une bacterie du genre brevibacterium

Legal Events

Date Code Title Description
AHS Application shelved for other reasons than non-payment