RU2006114432A - Способы и композиции для определения функции гена - Google Patents

Способы и композиции для определения функции гена Download PDF

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Publication number
RU2006114432A
RU2006114432A RU2006114432/13A RU2006114432A RU2006114432A RU 2006114432 A RU2006114432 A RU 2006114432A RU 2006114432/13 A RU2006114432/13 A RU 2006114432/13A RU 2006114432 A RU2006114432 A RU 2006114432A RU 2006114432 A RU2006114432 A RU 2006114432A
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Russia
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gene
clones
infection
retroviral construct
capture
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RU2006114432/13A
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English (en)
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Гвенн ХАНСЕН (US)
Гвенн ХАНСЕН
Алехандро АБУИН (US)
Алехандро АБУИН
Брайан ЗАМБРОВИЧ (US)
Брайан Замбрович
Карл Йохан ФРИДЛ (US)
Карл Йохан ФРИДЛ
Уилль м П. ДЕМПСИ (US)
Уилльям П. ДЕМПСИ
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Лексикон Дженетикс Инкорпорейтед (Us)
Лексикон Дженетикс Инкорпорейтед
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Application filed by Лексикон Дженетикс Инкорпорейтед (Us), Лексикон Дженетикс Инкорпорейтед filed Critical Лексикон Дженетикс Инкорпорейтед (Us)
Publication of RU2006114432A publication Critical patent/RU2006114432A/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (10)

1. Способ получения коллекции отдельно охарактеризованных клонов клеток млекопитающих с инсерциями, включающий
a) инфицирование клеток млекопитающих ретровирусной конструкцией для "улавливания" гена со значением множественности заражения менее 5;
b) отбор клонов клеток млекопитающих, устойчиво содержащих интегрированную провирусную форму упомянутой ретровирусной конструкции для "улавливания" гена; и
c) идентификацию in vitro участка геномной ДНК, прилегающего к интегрированной провирусной форме упомянутой ретровирусной конструкции для "улавливания" гена, причем при идентификации не используется реакция обратной транскриптазы.
2. Способ по п.1, в котором значение упомянутой множественности заражения составляет менее 1.
3. Способ по п.2, в котором значение упомянутой множественности заражения составляет менее 0,5.
4. Способ по п.3, в котором упомянутую идентификацию проводят путем секвенирования, по крайней мере, 50 оснований геномной ДНК, прилегающих к интегрированной провирусной форме упомянутой ретровирусной конструкции для "улавливания" гена.
5. Способ по п.3, в котором отбирают коллекцию, по крайней мере, из 10000 различных клонов клеток млекопитающих.
6. Способ по п.5, в котором коллекция, по крайней мере, из 10000 различных клонов клеток млекопитающих включает, по крайней мере, 10000 различных клонов клеток млекопитающих, каждый из которых содержит в отличном от другого клона гене интегрированную провирусную форму упомянутой ретровирусной конструкции для "улавливания" гена.
7. Способ по п.1, в котором идентификация включает в себя проведение обратной полимеразной цепной реакции (IPCR).
8. Способ по п.7, в котором обратная полимеразная цепная реакция включает, по крайней мере, одну полимеразу, выбранную из Pfu, Taq, Isis, Vent, Pwo, Phusion и Tth.
9. Коллекция отдельно охарактеризованных клонов клеток млекопитающих с инсерциями, полученная в соответствии со способом по п. 1.
10. Способ по п.7, в котором обратная полимеразная цепная реакция не включает в себя полимеразу Phusion.
RU2006114432/13A 2003-09-30 2004-09-30 Способы и композиции для определения функции гена RU2006114432A (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50743703P 2003-09-30 2003-09-30
US60/507,437 2003-09-30

Publications (1)

Publication Number Publication Date
RU2006114432A true RU2006114432A (ru) 2007-11-20

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US (1) US20050118710A1 (ru)
EP (1) EP1678307A1 (ru)
JP (1) JP2007507224A (ru)
KR (1) KR20060092244A (ru)
CN (1) CN1860232A (ru)
AU (1) AU2004278685A1 (ru)
CA (1) CA2540244A1 (ru)
IL (1) IL174437A0 (ru)
MX (1) MXPA06003572A (ru)
RU (1) RU2006114432A (ru)
WO (1) WO2005033315A1 (ru)

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4190496A (en) * 1971-05-14 1980-02-26 Syva Company Homogeneous enzyme assay for antibodies
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1293460C (en) * 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
EP0400047B1 (en) * 1988-02-05 1997-04-23 Whitehead Institute For Biomedical Research Modified hepatocytes and uses therefor
WO1990002806A1 (en) * 1988-09-01 1990-03-22 Whitehead Institute For Biomedical Research Recombinant retroviruses with amphotropic and ecotropic host ranges
US5091310A (en) * 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5364783A (en) * 1990-05-14 1994-11-15 Massachusetts Institute Of Technology Retrovirus promoter-trap vectors
WO1992015694A1 (en) * 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US5968502A (en) * 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5340740A (en) * 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
US5690926A (en) * 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
US5652128A (en) * 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
WO1995032225A1 (en) * 1994-05-23 1995-11-30 The Salk Institute For Biological Studies Method for site-specific integration of nucleic acids and related products
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5789653A (en) * 1995-01-10 1998-08-04 University Of Edinburgh Secretory gene trap
GB9500423D0 (en) * 1995-01-10 1995-03-01 Univ Edinburgh Novel vectors and use thereof for capturing target genes
US5679523A (en) * 1995-11-16 1997-10-21 The Board Of Trustees Of The Leland Stanford Junior University Method for concurrent disruption of expression of multiple alleles of mammalian genes
US6139833A (en) * 1997-08-08 2000-10-31 Lexicon Genetics Incorporated Targeted gene discovery
US6207371B1 (en) * 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6136566A (en) * 1996-10-04 2000-10-24 Lexicon Graphics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6808921B1 (en) * 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6080576A (en) * 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery

Also Published As

Publication number Publication date
WO2005033315A1 (en) 2005-04-14
AU2004278685A1 (en) 2005-04-14
EP1678307A1 (en) 2006-07-12
JP2007507224A (ja) 2007-03-29
IL174437A0 (en) 2006-08-01
CN1860232A (zh) 2006-11-08
MXPA06003572A (es) 2006-08-31
US20050118710A1 (en) 2005-06-02
KR20060092244A (ko) 2006-08-22
CA2540244A1 (en) 2005-04-14

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