DE60043985D1 - "gen-fallen-vektor" und verfahren zum "einfangen" von genen mittels dieses vektors - Google Patents

"gen-fallen-vektor" und verfahren zum "einfangen" von genen mittels dieses vektors

Info

Publication number
DE60043985D1
DE60043985D1 DE60043985T DE60043985T DE60043985D1 DE 60043985 D1 DE60043985 D1 DE 60043985D1 DE 60043985 T DE60043985 T DE 60043985T DE 60043985 T DE60043985 T DE 60043985T DE 60043985 D1 DE60043985 D1 DE 60043985D1
Authority
DE
Germany
Prior art keywords
sequence
vector
inverted repeat
loxp
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60043985T
Other languages
English (en)
Inventor
K Yamamura
Kimi Araki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgenic Inc
Original Assignee
Transgenic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenic Inc filed Critical Transgenic Inc
Application granted granted Critical
Publication of DE60043985D1 publication Critical patent/DE60043985D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physical Deposition Of Substances That Are Components Of Semiconductor Devices (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60043985T 1999-07-14 2000-05-02 "gen-fallen-vektor" und verfahren zum "einfangen" von genen mittels dieses vektors Expired - Lifetime DE60043985D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP20099799 1999-07-14
PCT/JP2000/002916 WO2001005987A1 (fr) 1999-07-14 2000-05-02 Vecteur piege et procede de piegeage de genes utilisant ledit vecteur

Publications (1)

Publication Number Publication Date
DE60043985D1 true DE60043985D1 (de) 2010-04-22

Family

ID=16433801

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60043985T Expired - Lifetime DE60043985D1 (de) 1999-07-14 2000-05-02 "gen-fallen-vektor" und verfahren zum "einfangen" von genen mittels dieses vektors

Country Status (11)

Country Link
US (3) US7312075B1 (de)
EP (2) EP1201759B1 (de)
JP (2) JP4664554B2 (de)
KR (1) KR20020057943A (de)
CN (3) CN101210254A (de)
AT (1) ATE460492T1 (de)
AU (1) AU778719B2 (de)
CA (1) CA2379055A1 (de)
DE (1) DE60043985D1 (de)
HK (1) HK1048830A1 (de)
WO (1) WO2001005987A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7074611B2 (en) * 2001-04-27 2006-07-11 Gie-Cerbn, Centre Europeen De Recherche En Biologie Et En Medecine (Gie) Method for the stable inversion of DNA sequence by site-specific recombination and DNA vectors and transgenic cells thereof
DE10140030C1 (de) * 2001-08-16 2002-12-19 Gsf Forschungszentrum Umwelt Einsatz mutierter Erkennungssequenzen für mehrfache aufeinander folgende Rekombinase-vermittelte Rekombinationen in einem genetischen System
CN100370024C (zh) * 2003-05-12 2008-02-20 上海南方模式生物科技发展有限公司 一种建立小鼠全基因组突变胚胎干细胞库的方法
CN100427591C (zh) * 2003-08-06 2008-10-22 上海南方模式生物科技发展有限公司 一种筛选和建立小鼠发育相关基因突变es细胞库的方法
CN100427592C (zh) * 2003-08-06 2008-10-22 上海南方模式生物科技发展有限公司 一种建立小鼠全基因组突变es细胞库的方法
JP5481661B2 (ja) * 2008-10-23 2014-04-23 学校法人福岡大学 変異導入遺伝子作製方法
CA2868292A1 (en) 2012-03-27 2013-10-03 National University Corporation Kumamoto University Immunodeficient mouse with humanized liver
CN107429261A (zh) 2014-12-15 2017-12-01 应用干细胞有限公司 在细胞中迅速产生单一或多重报告基因的方法和组合物
CN107729300B (zh) * 2017-09-18 2021-12-24 百度在线网络技术（北京）有限公司 文本相似度的处理方法、装置、设备和计算机存储介质
CN108004264B (zh) * 2017-12-14 2020-09-08 华中科技大学 毕赤酵母基因敲除和抗性基因回收载体及构建方法与运用
CN110751982B (zh) * 2018-07-04 2023-11-10 广州赛业百沐生物科技有限公司 一种智能并行化敲除策略筛选的方法及系统
CN117051039B (zh) * 2023-07-19 2024-05-24 中国科学院水生生物研究所 两端包含LoxP位点的表达GFP蛋白的质粒及其构建方法和应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
US4468464A (en) 1974-11-04 1984-08-28 The Board Of Trustees Of The Leland Stanford Junior University Biologically functional molecular chimeras
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4736866B1 (en) 1984-06-22 1988-04-12 Transgenic non-human mammals
JPS6242239Y2 (de) * 1984-10-15 1987-10-29
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5378618A (en) 1988-04-15 1995-01-03 E. I. Du Pont De Nemours And Company Vitro headful packaging system for cloning DNA fragments as large as 95kb
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5300431A (en) 1991-02-26 1994-04-05 E. I. Du Pont De Nemours And Company Positive selection vector for the bacteriophage P1 cloning system
WO1993004169A1 (en) 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
GB9308271D0 (en) 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5928914A (en) * 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US6139833A (en) 1997-08-08 2000-10-31 Lexicon Genetics Incorporated Targeted gene discovery
US6207371B1 (en) 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6037125A (en) 1996-11-05 2000-03-14 Lexicon Genetics Incorporated Disruption of the mammalian RAD51 protein and disruption of proteins that associate with mammalian RAD51 for hindering cell proliferation and/or viability of proliferating cells
US5994620A (en) 1996-12-10 1999-11-30 The Jackson Laboratory Induced chromosomal deletion
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
CA2205888A1 (en) * 1997-07-11 1999-01-11 John J. Priatel Complementation trap
JP2001513991A (ja) 1997-08-26 2001-09-11 レキシコン ジェネティックス インコーポレーテッド 細胞および非ヒトトランスジェニック動物における減損ｂｒｃａ２機能
JPH11200997A (ja) 1998-01-08 1999-07-27 Nippon Soken Inc 燃料噴射装置
US6436707B1 (en) 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
JP3725782B2 (ja) 1998-03-27 2005-12-14 レキシコン ジェネティックス インコーポレーテッド 遺伝子突然変異誘発および遺伝子発見のためのベクター
US6080576A (en) 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
CA2339041A1 (en) 1998-08-10 2000-02-24 Lexicon Genetics Incorporated Construction of normalized cdna libraries from eucaryotic cells

Also Published As

Publication number Publication date
WO2001005987A1 (fr) 2001-01-25
CA2379055A1 (en) 2001-01-25
EP1201759B1 (de) 2010-03-10
KR20020057943A (ko) 2002-07-12
US20090202988A1 (en) 2009-08-13
EP2031068A1 (de) 2009-03-04
AU4317600A (en) 2001-02-05
CN101210254A (zh) 2008-07-02
ATE460492T1 (de) 2010-03-15
CN1721529B (zh) 2013-03-13
CN1375012A (zh) 2002-10-16
EP1201759A1 (de) 2002-05-02
EP1201759A4 (de) 2002-07-31
US8722408B2 (en) 2014-05-13
CN1375012B (zh) 2010-05-26
CN1721529A (zh) 2006-01-18
US20110117642A1 (en) 2011-05-19
US7312075B1 (en) 2007-12-25
AU778719B2 (en) 2004-12-16
JP2011045380A (ja) 2011-03-10
JP4664554B2 (ja) 2011-04-06
HK1048830A1 (en) 2003-04-17

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