DK2644707T3 - RNase-H-based assays using modified RNA monomers. - Google Patents
RNase-H-based assays using modified RNA monomers. Download PDFInfo
- Publication number
- DK2644707T3 DK2644707T3 DK13173388.3T DK13173388T DK2644707T3 DK 2644707 T3 DK2644707 T3 DK 2644707T3 DK 13173388 T DK13173388 T DK 13173388T DK 2644707 T3 DK2644707 T3 DK 2644707T3
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- DK
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- Prior art keywords
- primer
- rnase
- cleavage
- dna
- enzyme
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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Families Citing this family (79)
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| EP3150727B1 (en) | 2008-04-30 | 2019-07-10 | Integrated DNA Technologies Inc. | Rnase-h-based assays utilizing modified rna monomers |
| US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
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| WO2010111682A2 (en) | 2009-03-27 | 2010-09-30 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| EP2488664A4 (en) * | 2009-10-13 | 2014-04-09 | Syntezza Molecular Detection Israel Ltd | METHOD AND COMPOSITIONS FOR REINFORCING TARGET SEQUENCES FROM NUCLEIC ACID SAMPLES |
| WO2011060014A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| JP2011188856A (ja) * | 2010-03-11 | 2011-09-29 | Samsung Techwin Co Ltd | リアルタイムｐｃｒのための核酸テンプレートの製造 |
| US8618253B2 (en) | 2010-05-25 | 2013-12-31 | Samsung Techwin Co., Ltd. | Modified RNAse H and detection of nucleic acid amplification |
| CN103210092B (zh) * | 2010-06-14 | 2015-11-25 | 新加坡国立大学 | 修饰的茎-环寡核苷酸介导的反转录和碱基间隔限制的定量pcr |
| US12043866B2 (en) | 2010-08-13 | 2024-07-23 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| US20120052482A1 (en) * | 2010-08-27 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting hepatitis c virus and method of detecting hepatitis c virus using the same |
| US20120052500A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting chlamydia trachomatis strains and method for detecting chlamydia trachomatis strains using the same |
| US20120052495A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria monocytogenes and use thereof |
| US20120052501A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting htlv strains and use thereof |
| US20120052492A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria spp. and use thereof |
| US20120052503A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting neisseria gonorrhoeae strains and method for detecting neisseria gonorrhoeae strains using the same |
| US20120088246A1 (en) * | 2010-10-07 | 2012-04-12 | Samsung Techwin Co., Ltd. | Real time pcr detection of single nucleotide polymorphisms |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| WO2012097353A1 (en) * | 2011-01-14 | 2012-07-19 | Life Technologies Corporation | Methods, compositions, and kits for detecting rare cells |
| EP2670894B1 (en) | 2011-02-02 | 2017-11-29 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| EP2721155A4 (en) * | 2011-06-15 | 2014-12-31 | Nse Products Inc | IDENTIFICATION OF CALORIC RESTRICTION AND MIMETIC RESTRICTION MARKERS |
| US9603949B2 (en) | 2011-09-01 | 2017-03-28 | University Of Iowa Research Foundation | Oligonucleotide-based probes for detection of bacterial nucleases |
| EP2814975A4 (en) * | 2012-02-14 | 2015-12-30 | Great Basin Scient | PROCESS FOR ISOTHERMAL AMPLIFICATION WITH BLOCKED PRIMERS |
| NZ701145A (en) | 2012-04-09 | 2016-09-30 | Envirologix Inc | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| US20130310269A1 (en) * | 2012-05-04 | 2013-11-21 | Austin So | Hot-start digital pcr |
| PT3539666T (pt) | 2012-05-24 | 2021-09-23 | Univ Utah Res Found | Pcr extrema |
| DK3428290T3 (da) | 2012-07-26 | 2022-07-04 | Illumina Inc | Sammensætninger og fremgangsmåder til amplifikation af nukleinsyrer |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
| US10086093B2 (en) | 2013-02-28 | 2018-10-02 | The General Hospital Corporation | miRNA profiling compositions and methods of use |
| CA2898456C (en) | 2013-03-13 | 2020-11-10 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| US9347095B2 (en) | 2013-03-15 | 2016-05-24 | Bio-Rad Laboratories, Inc. | Digital assays for mutation detection |
| GB2525568B (en) | 2013-03-15 | 2020-10-14 | Abvitro Llc | Single cell barcoding for antibody discovery |
| EP2971072A1 (en) * | 2013-03-15 | 2016-01-20 | Integrated DNA Technologies Inc. | Rnase h-based assays utilizing modified rna monomers |
| CA2930595C (en) * | 2013-11-14 | 2023-09-19 | Integrated Dna Technologies, Inc. | Dna polymerase mutants having enhanced template discrimination activity |
| WO2015085183A2 (en) * | 2013-12-06 | 2015-06-11 | Swift Biosciences, Inc. | Cleavable competitor polynucleotides |
| AU2014364926B2 (en) | 2013-12-20 | 2017-12-14 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic DNA samples |
| CA2939124C (en) | 2014-02-07 | 2023-08-29 | University Of Iowa Research Foundation | Oligonucleotide-based probes and methods for detection of microbes |
| GB201403216D0 (en) * | 2014-02-24 | 2014-04-09 | Cambridge Epigenetix Ltd | Nucleic acid sample preparation |
| EP3134551B1 (en) | 2014-04-22 | 2020-06-03 | Envirologix Inc. | Compositions and methods for enhancing and/or predicting dna amplification |
| EP4613872A3 (en) | 2014-08-11 | 2025-11-12 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| WO2016025878A1 (en) * | 2014-08-14 | 2016-02-18 | Abbott Molecular Inc. | Multifunctional oligonucleotides |
| NZ730044A (en) | 2014-09-15 | 2024-08-30 | Abvitro Llc | High-throughput nucleotide library sequencing |
| CA2964799A1 (en) | 2014-10-17 | 2016-04-21 | Illumina Cambridge Limited | Contiguity preserving transposition |
| BR112017008082A2 (pt) | 2014-10-20 | 2017-12-26 | Envirologix Inc | composições e métodos para detectar um vírus de rna |
| CN107208143A (zh) * | 2014-11-20 | 2017-09-26 | 安普里怀斯公司 | 用于核酸扩增和分析的组合物、方法、系统和试剂盒 |
| JP6718881B2 (ja) * | 2015-03-06 | 2020-07-08 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ． ＬＬＣ | 核酸増幅およびライブラリー調製 |
| AU2016313095A1 (en) * | 2015-08-24 | 2017-11-23 | Qiagen Gmbh | Method for generating a RNA-sequencing library |
| CA2999886A1 (en) | 2015-09-24 | 2017-03-30 | Abvitro Llc | Single amplicon activated exclusion pcr |
| EP3933047A1 (en) | 2015-09-24 | 2022-01-05 | AbVitro LLC | Affinity-oligonucleotide conjugates and uses thereof |
| EP3353550A1 (en) | 2015-09-25 | 2018-08-01 | AbVitro LLC | High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences |
| DK3337615T3 (da) * | 2015-11-05 | 2022-05-09 | Univ Utah Res Found | Ekstrem pcr via revers transkription |
| JP6744917B2 (ja) * | 2015-11-25 | 2020-08-19 | インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド | バリアント検出のための方法 |
| US20210395799A1 (en) * | 2015-11-25 | 2021-12-23 | Integrated Dna Technologies, Inc. | Methods for variant detection |
| US20170218438A1 (en) * | 2016-02-01 | 2017-08-03 | Integrated Dna Technologies, Inc. | Cleavable primers for isothermal amplification |
| CN107083427B (zh) * | 2016-04-01 | 2021-07-30 | 广州市基准医疗有限责任公司 | Dna连接酶介导的dna扩增技术 |
| WO2017214561A1 (en) * | 2016-06-10 | 2017-12-14 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| BR112019005748A2 (pt) | 2016-09-24 | 2019-06-18 | Abvitro Llc | afinidade-conjugados de oligonucleotídeo e usos destes |
| WO2018190894A1 (en) | 2017-04-13 | 2018-10-18 | Integrated Dna Technologies, Inc. | Methods for variant detection |
| EP3631012B1 (en) | 2017-05-26 | 2022-06-08 | AbVitro LLC | High-throughput polynucleotide library sequencing and transcriptome analysis |
| WO2018231985A1 (en) * | 2017-06-13 | 2018-12-20 | Genetics Research, Llc, D/B/A Zs Genetics, Inc. | Polynucleic acid molecule enrichment methodologies |
| US10081829B1 (en) | 2017-06-13 | 2018-09-25 | Genetics Research, Llc | Detection of targeted sequence regions |
| US10527608B2 (en) | 2017-06-13 | 2020-01-07 | Genetics Research, Llc | Methods for rare event detection |
| WO2019051335A1 (en) | 2017-09-07 | 2019-03-14 | Juno Therapeutics, Inc. | METHODS OF IDENTIFYING CELLULAR CHARACTERISTICS RELATED TO RESPONSES ASSOCIATED WITH CELL THERAPY |
| EP3688181A4 (en) | 2017-09-29 | 2021-06-16 | University of Iowa Research Foundation | COMPOSITIONS AND METHOD OF DETECTING DIGITAL NUCLEASES |
| WO2019226734A1 (en) * | 2018-05-22 | 2019-11-28 | Biochain Institute, Inc. | Target enrichment and sequencing of modified nucleic acid for human cancer detection |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| CN109913538A (zh) * | 2018-09-06 | 2019-06-21 | 湖南融健基因生物科技有限公司 | 一种快速检测snp位点的荧光定量试剂盒 |
| US11591629B2 (en) * | 2019-12-23 | 2023-02-28 | Cheng-Yao Chen | Method and kit for template-independent nucleic acid synthesis |
| KR20210109745A (ko) * | 2020-02-28 | 2021-09-07 | 주식회사 누리바이오 | 단일 표적 유전자의 유전적 변이 실시간 검출용 단일핵산 및 이를 이용한 검출 방법 |
| CN115135664A (zh) * | 2020-06-22 | 2022-09-30 | 苏州新海生物科技股份有限公司 | 一种核酸配体及其应用 |
| CN113897420A (zh) * | 2020-06-22 | 2022-01-07 | 上海思路迪生物医学科技有限公司 | 基于扩增子测序的大片段重排检测的引物组合物、试剂盒和检测方法 |
| CN112063689A (zh) * | 2020-08-21 | 2020-12-11 | 上海思路迪生物医学科技有限公司 | 一种高效rna反转录方法及rna和dna同步建库方法 |
| AU2021409723A1 (en) * | 2020-12-24 | 2023-06-22 | Integrated Dna Technologies, Inc. | Rnase h2 mutants that reduce primer dimers and off-target amplification in rhpcr-based amplicon sequencing with high-fidelity dna polymerases |
| CN113981069B (zh) * | 2021-11-10 | 2023-08-15 | 郑州华沃生物科技有限公司 | 检测adrb1基因g1165c多态性的引物、试剂盒及其检测方法和应用 |
| CN115948388A (zh) * | 2022-12-30 | 2023-04-11 | 纳昂达(南京)生物科技有限公司 | 特异性捕获引物、靶向捕获探针组合物、靶向捕获文库的构建方法及应用 |
| WO2025096569A1 (en) * | 2023-10-30 | 2025-05-08 | The Penn State Research Foundation | Methods and compositions for real-time antimicrobial resistance profiling |
| WO2025207561A1 (en) * | 2024-03-27 | 2025-10-02 | Cepheid | Isothermal amplification using novel synthetic oligonucleotides |
| WO2025259863A1 (en) * | 2024-06-12 | 2025-12-18 | Orbital Therapeutics, Inc. | Thermostable ribonuclease r for circular rna purification |
Family Cites Families (85)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| CA1340522C (en) | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
| US6031091A (en) | 1987-09-21 | 2000-02-29 | Gen-Probe Incorporated | Non-nucleotide linking reagents for nucleotide probes |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5002867A (en) | 1988-04-25 | 1991-03-26 | Macevicz Stephen C | Nucleic acid sequence determination by multiple mixed oligonucleotide probes |
| DE68926504T2 (de) | 1988-07-20 | 1996-09-12 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| US5516663A (en) * | 1990-01-26 | 1996-05-14 | Abbott Laboratories | Ligase chain reaction with endonuclease IV correction and contamination control |
| AU653712B2 (en) | 1990-02-16 | 1994-10-13 | F. Hoffmann-La Roche Ag | Improvements in the specificity and convenience of the polymerase chain reaction |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| JPH0515439A (ja) | 1991-07-08 | 1993-01-26 | Matsushita Electric Ind Co Ltd | ジヤー炊飯器の制御回路 |
| EP0655090B1 (en) | 1992-04-27 | 2000-12-27 | The Trustees Of Dartmouth College | Detection of gene sequences in biological fluids |
| CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| ES2165402T3 (es) * | 1994-07-16 | 2002-03-16 | Roche Diagnostics Gmbh | Procedimiento para la deteccion sensible de acidos nucleicos. |
| FR2724934B1 (fr) | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
| WO1996015271A1 (en) * | 1994-11-16 | 1996-05-23 | Abbott Laboratories | Multiplex ligations-dependent amplification |
| US20030165908A1 (en) | 1994-12-30 | 2003-09-04 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| EP0803058B1 (en) | 1994-12-30 | 2008-11-05 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5898031A (en) | 1996-06-06 | 1999-04-27 | Isis Pharmaceuticals, Inc. | Oligoribonucleotides for cleaving RNA |
| AU726821B2 (en) | 1996-07-16 | 2000-11-23 | Gen-Probe Incorporated | Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm |
| US5853990A (en) * | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| EP0866071B1 (en) | 1997-03-20 | 2004-10-20 | F. Hoffmann-La Roche Ag | Modified primers |
| US6548247B1 (en) | 1997-04-16 | 2003-04-15 | Trevigen, Inc. | Detection and mapping of point mutations using partial digestion |
| US5846726A (en) | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| EP1002128A1 (en) | 1997-07-11 | 2000-05-24 | Brax Genomics Limited | Characterising nucleic acids |
| JPH1132772A (ja) * | 1997-07-24 | 1999-02-09 | Mitsubishi Chem Corp | 耐熱性リボヌクレアーゼｈ及びそれをコードするｄｎａ |
| GB9725237D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
| JP2001525166A (ja) | 1997-12-04 | 2001-12-11 | アイシス・ファーマシューティカルス・インコーポレーテッド | ヒトｒｎａｓｅｈおよびその組成物および使用 |
| WO2005021776A2 (en) * | 2003-08-21 | 2005-03-10 | Isis Pharmaceuticals, Inc. | METHODS OF USING MAMMALIAN RNase H AND COMPOSITIONS THEREOF |
| US6248877B1 (en) | 1998-04-07 | 2001-06-19 | Biolink Partners | Solid phase synthesis of organic compounds via phosphitylating reagents |
| EP1159453B1 (en) * | 1999-03-10 | 2008-05-28 | ASM Scientific, Inc. | A method for direct nucleic acid sequencing |
| PT1218542E (pt) | 1999-09-13 | 2004-08-31 | Nugen Technologies Inc | Metodos e composicoes para amplificacao isotermica linear de sequencias polinucleotidicas |
| US6274353B1 (en) * | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
| JP4949585B2 (ja) | 1999-10-29 | 2012-06-13 | アジレント・テクノロジーズ・インク | Ｄｎａポリメラーゼを使用した組成物および方法 |
| EP1925669B1 (en) | 2000-02-17 | 2010-12-08 | Qiagen GmbH | Thermostable chimeric nucleic acid polymerases and uses thereof |
| EP1259646B1 (en) | 2000-02-23 | 2009-09-02 | City of Hope | Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination |
| US7033763B2 (en) | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
| US6596490B2 (en) | 2000-07-14 | 2003-07-22 | Applied Gene Technologies, Inc. | Nucleic acid hairpin probes and uses thereof |
| AU2001286211A1 (en) | 2000-09-14 | 2002-03-26 | Takara Bio Inc | Thermotolerant ribonuclease h |
| EP1427847B1 (en) | 2000-12-13 | 2010-07-28 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| CN100351393C (zh) * | 2001-02-15 | 2007-11-28 | 宝生物工程株式会社 | 核苷酸多态性的检测方法 |
| US6596489B2 (en) | 2001-03-30 | 2003-07-22 | Applied Gene Technologies | Methods and compositions for analyzing nucleotide sequence mismatches using RNase H |
| EP1275735A1 (en) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
| JPWO2003016569A1 (ja) | 2001-08-20 | 2004-12-02 | タカラバイオ株式会社 | 核酸の増幅方法 |
| CA2409775C (en) * | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
| AU2003213652A1 (en) | 2002-03-01 | 2003-09-16 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
| US7629152B2 (en) | 2002-03-01 | 2009-12-08 | Integrated Dna Technologies, Inc. | Methods for amplifying polymeric nucleic acids |
| JP2006509040A (ja) * | 2002-08-23 | 2006-03-16 | ソレックサ リミテッド | 修飾されたヌクレオチド |
| EP1553172B1 (en) * | 2002-08-30 | 2009-06-03 | Takara Bio Inc. | Thermostable ribonuclease h |
| WO2004048594A2 (en) | 2002-11-21 | 2004-06-10 | Epicentre Technologies | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
| US20040175737A1 (en) * | 2002-12-23 | 2004-09-09 | Wyeth | Assay for RNase H activity |
| JP2005006587A (ja) * | 2003-06-20 | 2005-01-13 | Takara Bio Inc | 標的核酸の増幅及び／又は検出方法 |
| AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| WO2005049849A2 (en) | 2003-11-14 | 2005-06-02 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
| KR101041106B1 (ko) | 2003-11-25 | 2011-06-13 | 한면기 | 핵산과 단백질의 신규한 실시간 탐지방법 |
| US7344834B2 (en) | 2003-12-08 | 2008-03-18 | Cytyc Corporation | Method for DNA amplification using DNA blocking probes |
| KR100968901B1 (ko) * | 2003-12-10 | 2010-07-14 | 다카라 바이오 가부시키가이샤 | 핵산의 증폭방법 |
| CN101426908B (zh) * | 2004-06-09 | 2014-06-11 | 纽星实验室 | 可逆性修饰的热稳定酶组合物及其制备方法和使用方法 |
| EP1825000A4 (en) | 2004-11-23 | 2009-05-13 | Xiao Bing Wang | DETECTION OF NUCLEIC ACID VARIATIONS BY CLEANING REINFORCEMENT PROCEDURES |
| JPWO2006070666A1 (ja) * | 2004-12-28 | 2008-06-12 | タカラバイオ株式会社 | 遺伝子多型の同時検出方法 |
| WO2006074233A2 (en) * | 2005-01-06 | 2006-07-13 | Applera Corporation | Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification |
| US7700287B2 (en) * | 2005-01-28 | 2010-04-20 | Life Technologies Corporation | Compositions and methods for terminating a sequencing reaction at a specific location in a target DNA template |
| US20080003571A1 (en) | 2005-02-01 | 2008-01-03 | Mckernan Kevin | Reagents, methods, and libraries for bead-based sequencing |
| US20060211024A1 (en) | 2005-03-10 | 2006-09-21 | Gwc Technologies Incorporated | Methods for analysis of a nucleic acid sample |
| US20090068643A1 (en) | 2005-11-23 | 2009-03-12 | Integrated Dna Technologies, Inc. | Dual Function Primers for Amplifying DNA and Methods of Use |
| AU2005338632B2 (en) * | 2005-11-30 | 2010-05-20 | Epicentre Technologies Corporation | Selective terminal tagging of nucleic acids |
| US20070238096A1 (en) | 2006-03-09 | 2007-10-11 | The Regents Of The University Of California | Hybrid energy transfer for nucleic acid detection |
| US20070218490A1 (en) | 2006-03-15 | 2007-09-20 | Integrated Dna Technologies, Inc. | Nucleic acid monomers with 2'-chemical moieties |
| CN101517091B (zh) | 2006-06-01 | 2013-02-13 | 垂林克生物技术公司 | 用于核酸扩增的化学修饰的寡核苷酸引物 |
| GB0611444D0 (en) * | 2006-06-09 | 2006-07-19 | Medical Res Council Technology | Rnase H2 complex and genes therefor |
| EP2029780A4 (en) * | 2006-06-16 | 2010-03-31 | Pacific Biosciences California | CONTROLLED INITIATION OF A PRIMARY EXTENSION |
| JP2008072427A (ja) | 2006-09-14 | 2008-03-27 | Konica Minolta Business Technologies Inc | 画像形成装置及びクライアント／サーバ型情報処理システム並びに情報処理方法 |
| EP1921156A1 (en) | 2006-11-10 | 2008-05-14 | bioMerieux B.V. | Improved multiplex nucleic acid amplification using blocked primers |
| EP3150727B1 (en) | 2008-04-30 | 2019-07-10 | Integrated DNA Technologies Inc. | Rnase-h-based assays utilizing modified rna monomers |
| WO2011060014A1 (en) | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| US8618253B2 (en) | 2010-05-25 | 2013-12-31 | Samsung Techwin Co., Ltd. | Modified RNAse H and detection of nucleic acid amplification |
| US20120052495A1 (en) | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria monocytogenes and use thereof |
| US20120052501A1 (en) | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting htlv strains and use thereof |
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