DK2644707T3 - RNase-H-baserede assays, som anvender modificerede RNA-monomere. - Google Patents
RNase-H-baserede assays, som anvender modificerede RNA-monomere. Download PDFInfo
- Publication number
- DK2644707T3 DK2644707T3 DK13173388.3T DK13173388T DK2644707T3 DK 2644707 T3 DK2644707 T3 DK 2644707T3 DK 13173388 T DK13173388 T DK 13173388T DK 2644707 T3 DK2644707 T3 DK 2644707T3
- Authority
- DK
- Denmark
- Prior art keywords
- primer
- rnase
- cleavage
- dna
- enzyme
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Saccharide Compounds (AREA)
Claims (22)
1. Fremgangsmåde til amplificering af en mål-DNA-sekvens, hvor fremgangsmåden omfatter trinnene at: a) tilvejebringe en reaktionsblanding omfattende (i) en oligonukleotidprimer, der har et spaltningsdomæne positioneret 5' af en blokeringsgruppe, hvor blokeringsgruppen er bundet på eller i nærheden af enden af 3'-enden af oligonukleotidprimeren, hvor blokeringsgruppen inhiberer primerforlængelse og/eller pri-meren fra at tjene som en skabelon til DNA-syntese, (ii) en prøvenukleinsyre som måske eller måske ikke har målsekvensen, (iii) et spaltningsenzym, hvor spaltningsenzymet er et termostabilt RNase-H2-enzym, som opnår øget aktivitet ved de forhøjede temperaturer benyttet i reaktionen og har reduceret aktivitet ved lavere temperaturer, og (iv) en polymerase; b) hybridisere primeren til mål-DNA-sekvensen for at danne et dobbeltstrenget substrat; c) spalte den hybridiserede primer med spaltningsenzymet på et sted indenfor eller tilstødende til spaltningsdomænet for at fjerne blokeringsgruppen fra primeren; og d) forlænge primeren med polymerasen.
2. Fremgangsmåde ifølge krav 1, hvor RNase-FI2-enzymet er reversibelt inaktiveret ved kemisk modifikation eller med et blokeringsantistof.
3. Fremgangsmåde ifølge krav 1, hvor blokeringsgruppen er (a) bundet til 3'-terminalnukleotidet af primeren, eller (b) er bundet 5' af 3'-terminalresten.
4. Fremgangsmåde ifølge krav 3(b), hvor blokeringsgruppen indbefatter én eller flere abasiske rester eller modificerede nukleosider, fortrinsvis er den abasiske rest en C3-spacer og det modificerede nukleosid er en 2'-0-methyl-riboserest.
5. Fremgangsmåde ifølge krav 1, hvor blokeringsgruppen indbefatter et mærke, som tillader detektion af amplifikationsreaktionen, fortrinsvis er mærket en fluorofor, et slukningsmiddel (ENG: quencher), biotin, en hapten eller en masse-markør til detektion af amplifikationsreaktionen ved massespektrometri.
6. Fremgangsmåde ifølge krav 1, hvor spaltningsdomænet er en kontinuerlig sekvens af 3 eller flere RNA-rester, fortrinsvis omfatter spaltningsdomænet yderligere én eller flere af de følgende dele: en DNA-rest, en abasisk rest, et modificeret nukleosid, eller en modificeret phosphat-internukleotidbinding.
7. Fremgangsmåde ifølge krav 2, hvor spaltningsdomænet er én enkelt RNA-rest eller to tilstødende RNA-rester.
8. Fremgangsmåde ifølge krav 2, hvor spaltningsdomænet mangler en RNA-rest, fortrinsvis omfatter spaltningsdomænet ét eller flere 2'-modificerede nukleosider.
9. Fremgangsmåde ifølge krav 8, hvor det 2'-modificerede nukleosid er ét enkelt 2'-fluornukleosid, eller, hvor spaltningsdomænet er to tilstødende 2'-fluornukleosidrester.
10. Fremgangsmåde ifølge krav 8, hvor spaltningsreaktionen udføres under tilstede- værelse af én eller flere af de følgende divalente kationer: mangan, cobalt, nikkel eller zink, eventuelt, er magnesium også tilstede i reaktionsblandingen.
11. Fremgangsmåde ifølge krav 7, hvor en sekvens indenfor eller flankerende spaltningsdomænet, indeholder én eller flere internukleosidbindinger resistente over for nuklea-sespaltning, fortrinsvis er den nukleaseresistente binding phosphorthioat, phosphor-dithioat, methylphosphonat eller en abasisk rest og er eventuelt på 3'-siden af spaltningsdomænet.
12. Fremgangsmåde ifølge krav 1, yderligere omfattende en anden primer i modsat orientering af den første primer til at støtte PCR, fortrinsvis er den anden primer en ikke-modificeret DNA-primer og eventuelt omfattende et spaltningsdomæne positioneret 5' af en blokeringsgruppe, blokeringsgruppen er bundet på eller i nærheden af enden af 3'-enden af oligonukleotidprimeren, hvor blokeringsgruppen forhindrer primerforlængelse.
13. Fremgangsmåde ifølge krav 12, hvor PCR-assayet anvendes til at: (a) skelne mellem variant-alleler; eller (b) kvantificere hyppigheden af mål-nukleinsekvensen i prøven.
14. Fremgangsmåde ifølge krav 13, hvor (a) et sekundært mutationssted er inkorporeret indenfor eller flankerende spaltningsdomænet for at forøge detektion af variant-allelen, eller (b) et modificeret nukleosid er inkorporeret indenfor eller flankerende spaltningsdomænet for at forøge detektion af variant-allelen, fortrinsvis er det modificerede nukleosid en 2'-0-methyl-riboserest.
15. Fremgangsmåde ifølge krav 13, hvor en nukleaseresistent binding er inkorporeret på 3'-siden af spaltningsdomænet.
16. Fremgangsmåde ifølge krav 12, hvor PCR-assayet er et primer-probe-PCR-assay.
17. Fremgangsmåde ifølge krav 16, hvor primeren, der har et 5'-mærkedomæne, indbefatter et spaltningsdomæne, fortrinsvis et RNase-H-spaltningsdomæne, mere fortrinsvis et RNase-H2-spaltningsdomæne.
18. Fremgangsmåde til amplificering af mål-DNA-sekvens, hvor fremgangsmåden omfatter trinnene at: a) tilvejebringe en reaktionsblanding omfattende: (i) en oligonukleotidprimer, der har et spaltningsdomæne positioneret 5' af en blokeringsgruppe, blokeringsgruppen er bundet opstrøms for 5' af 3'-terminalresten af oligonukleotidprimeren, hvor blokeringsgruppen inhiberer primerforlængelse og/eller primeren fra at tjene som en skabelon til DNA-syntese, (ii) en prøvenukleinsyre som måske eller måske ikke har målsekvensen, (iii) et termostabilt RNase-H2-enzym som opnår øget aktivitet ved de forhøjede temperaturer i reaktionen og har reduceret aktivitet ved lavere temperaturer og (iv) en polymerase; b) hybridisere primeren til mål-DNA-sekvensen for at danne et dobbeltstrenget substrat; c) spalte den hybridiserede primer med det termostabile RNase-H2-enzym på et sted indenfor eller tilstødende til spaltningsdomænet for at fjerne blokeringsgruppen fra prime-ren; og d) forlænge primeren med polymerasen.
19. Fremgangsmåde ifølge krav 7 eller 18, hvor spaltningsdomænet er én enkelt RNA-rest og blokeringsgruppen omfatter to eller flere abasiske rester.
20. Fremgangsmåde ifølge krav 18 eller 19, hvor en region 3' til RNA-resten af primeren omfatter en sekvens Dl-x-x-D2, hvor Dl og D2 er DNA-baser og x er en abasisk rest.
21. Anvendelse af en primer til DNA-replikation, der har en 5'-ende og en 3'-ende, primeren omfattende: a) en ikke-aktiveret konfiguration når primeren er ikke-hybridiseret til en DNA-sekvens af interesse, den ikke-aktiverede konfiguration omfattende: (i) et RNase-FI2-spaltningsdomæne omfattende én eller flere 2'-modificerede nukleo-sidrester, og en blokeringsgruppe bundet på eller i nærheden af 3'-enden af primeren; eller (ii) et RNase-FI2-spaltningsdomæne, og en blokeringsgruppe bundet på eller i nærheden af 3'-enden af primeren, som inkorporerer et mærke; eller (iii) et RNase-FI2-spaltningsdomæne, og en blokeringsgruppe placeret 5' til 3'-terminalnukleotidresten af primeren; og b) en aktiveret konfiguration når primeren er hybridiseret til DNA-sekvensen af interesse, hvor den aktiverede konfiguration er fri af blokeringsgruppen og er i stand til at støtte DNA-replikation; i fremgangsmåden ifølge et hvilket som helst af krav 1-20.
22. Anvendelse ifølge krav 21, hvor den 2'-modificerede nukleosidrest af (i) er en 2'-fluornukleosid.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4920408P | 2008-04-30 | 2008-04-30 | |
EP09739895.2A EP2279263B1 (en) | 2008-04-30 | 2009-04-30 | Rnase-h-based assays utilizing modified rna monomers |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2644707T3 true DK2644707T3 (da) | 2015-06-29 |
Family
ID=41203821
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK13173388.3T DK2644707T3 (da) | 2008-04-30 | 2009-04-30 | RNase-H-baserede assays, som anvender modificerede RNA-monomere. |
DK09739895.2T DK2279263T3 (da) | 2008-04-30 | 2009-04-30 | Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK09739895.2T DK2279263T3 (da) | 2008-04-30 | 2009-04-30 | Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer |
Country Status (7)
Country | Link |
---|---|
US (4) | US20090325169A1 (da) |
EP (6) | EP2644707B1 (da) |
JP (6) | JP5539325B2 (da) |
AU (1) | AU2009242546B2 (da) |
CA (1) | CA2722541C (da) |
DK (2) | DK2644707T3 (da) |
WO (1) | WO2009135093A2 (da) |
Families Citing this family (70)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
WO2009135093A2 (en) | 2008-04-30 | 2009-11-05 | Integrated Dna Technologies, Inc. | Rnase-h-based assays utilizing modified rna monomers |
US20100285478A1 (en) | 2009-03-27 | 2010-11-11 | Life Technologies Corporation | Methods, Compositions, and Kits for Detecting Allelic Variants |
EP2488664A4 (en) * | 2009-10-13 | 2014-04-09 | Syntezza Molecular Detection Israel Ltd | METHOD AND COMPOSITIONS FOR REINFORCING TARGET SEQUENCES FROM NUCLEIC ACID SAMPLES |
US20110117559A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
JP2011188856A (ja) * | 2010-03-11 | 2011-09-29 | Samsung Techwin Co Ltd | リアルタイムpcrのための核酸テンプレートの製造 |
US8618253B2 (en) * | 2010-05-25 | 2013-12-31 | Samsung Techwin Co., Ltd. | Modified RNAse H and detection of nucleic acid amplification |
WO2011159256A1 (en) * | 2010-06-14 | 2011-12-22 | National University Of Singapore | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr |
US20120052482A1 (en) * | 2010-08-27 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting hepatitis c virus and method of detecting hepatitis c virus using the same |
US20120052500A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting chlamydia trachomatis strains and method for detecting chlamydia trachomatis strains using the same |
US20120052492A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria spp. and use thereof |
US20120052503A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting neisseria gonorrhoeae strains and method for detecting neisseria gonorrhoeae strains using the same |
US20120052495A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria monocytogenes and use thereof |
US20120052501A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting htlv strains and use thereof |
US20120088246A1 (en) * | 2010-10-07 | 2012-04-12 | Samsung Techwin Co., Ltd. | Real time pcr detection of single nucleotide polymorphisms |
US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
US20120214160A1 (en) * | 2011-01-14 | 2012-08-23 | Life Technologies Corporation | Methods, compositions, and kits for detecting rare cells |
CN103443338B (zh) | 2011-02-02 | 2017-09-22 | 华盛顿大学商业化中心 | 大规模平行邻接作图 |
CN107217051A (zh) * | 2011-06-15 | 2017-09-29 | Nse产品公司 | 识别热量限制标记物和热量限制模拟物 |
EP2751567B1 (en) | 2011-09-01 | 2019-05-08 | University of Iowa Research Foundation | Oligonucleotide-based probes for detection of bacterial nucleases |
CA2881200A1 (en) * | 2012-02-14 | 2013-08-22 | Great Basin Scientific | Methods of isothermal amplification using blocked primers |
US9096897B2 (en) | 2012-04-09 | 2015-08-04 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide |
WO2013166466A1 (en) * | 2012-05-04 | 2013-11-07 | Bio-Rad Laboratories, Inc. | Hot-start digital pcr |
PT2855692T (pt) | 2012-05-24 | 2019-10-25 | Univ Utah Res Found | Pcr extrema. |
ES2917400T3 (es) * | 2012-07-26 | 2022-07-08 | Illumina Inc | Composiciones y métodos para la amplificación de ácidos nucleicos |
US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
US10086093B2 (en) | 2013-02-28 | 2018-10-02 | The General Hospital Corporation | miRNA profiling compositions and methods of use |
AU2013382098B2 (en) | 2013-03-13 | 2019-02-07 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
WO2014152054A1 (en) | 2013-03-15 | 2014-09-25 | Bio-Rad Laboratories, Inc. | Digital assays for mutation detection |
GB2584364A (en) | 2013-03-15 | 2020-12-02 | Abvitro Llc | Single cell bar-coding for antibody discovery |
CA2906365A1 (en) * | 2013-03-15 | 2014-09-18 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
CA3207128A1 (en) * | 2013-11-14 | 2015-05-21 | Integrated Dna Technologies, Inc. | Dna polymerase mutants having enhanced template discrimination activity |
US10385388B2 (en) | 2013-12-06 | 2019-08-20 | Swift Biosciences, Inc. | Cleavable competitor polynucleotides |
DK3083994T3 (da) | 2013-12-20 | 2021-09-13 | Illumina Inc | Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver |
WO2015120406A1 (en) * | 2014-02-07 | 2015-08-13 | University Of Iowa Research Foundation | Oligonucleotide-based probes and methods for detection of microbes |
GB201403216D0 (en) * | 2014-02-24 | 2014-04-09 | Cambridge Epigenetix Ltd | Nucleic acid sample preparation |
JP6596442B2 (ja) | 2014-04-22 | 2019-10-23 | エンバイロロジックス インコーポレイテッド | Dna増幅を増強および/または予測するための組成物および方法 |
CA2956812C (en) | 2014-08-11 | 2023-04-11 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
CA2955967A1 (en) * | 2014-08-14 | 2016-02-18 | Abbott Molecular Inc. | Multifunctional oligonucleotides |
EP3536786B1 (en) | 2014-09-15 | 2021-06-30 | AbVitro LLC | High-throughput nucleotide library sequencing |
RU2709655C2 (ru) | 2014-10-17 | 2019-12-19 | Иллумина Кембридж Лимитед | Транспозиция с сохранением сцепления генов |
CN114606228A (zh) | 2014-11-20 | 2022-06-10 | 安普里怀斯公司 | 用于核酸扩增的组合物和方法 |
CN107406884A (zh) * | 2015-03-06 | 2017-11-28 | 西格马-奥尔德里奇有限责任公司 | 核酸扩增和文库制备 |
JP6886962B2 (ja) * | 2015-08-24 | 2021-06-16 | キアゲン ゲーエムベーハー | Rnaシークエンシングライブラリーを生成する方法 |
CA2999886A1 (en) | 2015-09-24 | 2017-03-30 | Abvitro Llc | Single amplicon activated exclusion pcr |
AU2016326737B2 (en) | 2015-09-24 | 2023-01-12 | Abvitro Llc | Affinity-oligonucleotide conjugates and uses thereof |
WO2017053902A1 (en) | 2015-09-25 | 2017-03-30 | Abvitro Llc | High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences |
CN108367287B (zh) | 2015-11-05 | 2021-04-02 | 犹他州大学研究基金会 | 极端逆转录pcr |
US20210395799A1 (en) * | 2015-11-25 | 2021-12-23 | Integrated Dna Technologies, Inc. | Methods for variant detection |
CN108291253A (zh) | 2015-11-25 | 2018-07-17 | 综合基因技术公司 | 用于变体检测的方法 |
US20170218438A1 (en) * | 2016-02-01 | 2017-08-03 | Integrated Dna Technologies, Inc. | Cleavable primers for isothermal amplification |
CN107083427B (zh) * | 2016-04-01 | 2021-07-30 | 广州市基准医疗有限责任公司 | Dna连接酶介导的dna扩增技术 |
US11268117B2 (en) | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
JP6929354B2 (ja) | 2016-09-24 | 2021-09-01 | アブビトロ, エルエルシー | 親和性−オリゴヌクレオチドコンジュゲートおよびその使用 |
WO2018190894A1 (en) | 2017-04-13 | 2018-10-18 | Integrated Dna Technologies, Inc. | Methods for variant detection |
US20200354784A1 (en) | 2017-05-26 | 2020-11-12 | Abvitro Llc | High-throughput polynucleotide library sequencing and transcriptome analysis |
US10081829B1 (en) | 2017-06-13 | 2018-09-25 | Genetics Research, Llc | Detection of targeted sequence regions |
US10527608B2 (en) | 2017-06-13 | 2020-01-07 | Genetics Research, Llc | Methods for rare event detection |
US20180355406A1 (en) * | 2017-06-13 | 2018-12-13 | Genetics Research, Llc, D/B/A Zs Genetics, Inc. | Polynucleic acid molecule enrichment methodologies |
EP3679370A1 (en) | 2017-09-07 | 2020-07-15 | Juno Therapeutics, Inc. | Methods of identifying cellular attributes related to outcomes associated with cell therapy |
CN112513265A (zh) * | 2018-05-22 | 2021-03-16 | 博诚研究中心 | 用于人类癌症检测的修饰核酸的靶向富集和测序 |
GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
CN109913538A (zh) * | 2018-09-06 | 2019-06-21 | 湖南融健基因生物科技有限公司 | 一种快速检测snp位点的荧光定量试剂盒 |
CN115135664A (zh) * | 2020-06-22 | 2022-09-30 | 苏州新海生物科技股份有限公司 | 一种核酸配体及其应用 |
CN113897420A (zh) * | 2020-06-22 | 2022-01-07 | 上海思路迪生物医学科技有限公司 | 基于扩增子测序的大片段重排检测的引物组合物、试剂盒和检测方法 |
CN112063689A (zh) * | 2020-08-21 | 2020-12-11 | 上海思路迪生物医学科技有限公司 | 一种高效rna反转录方法及rna和dna同步建库方法 |
CN113981069B (zh) * | 2021-11-10 | 2023-08-15 | 郑州华沃生物科技有限公司 | 检测adrb1基因g1165c多态性的引物、试剂盒及其检测方法和应用 |
CN115948388A (zh) * | 2022-12-30 | 2023-04-11 | 纳昂达(南京)生物科技有限公司 | 特异性捕获引物、靶向捕获探针组合物、靶向捕获文库的构建方法及应用 |
Family Cites Families (85)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
CA1340522C (en) | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
US6031091A (en) | 1987-09-21 | 2000-02-29 | Gen-Probe Incorporated | Non-nucleotide linking reagents for nucleotide probes |
US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
US5002867A (en) | 1988-04-25 | 1991-03-26 | Macevicz Stephen C | Nucleic acid sequence determination by multiple mixed oligonucleotide probes |
ATE138106T1 (de) | 1988-07-20 | 1996-06-15 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
US5516663A (en) * | 1990-01-26 | 1996-05-14 | Abbott Laboratories | Ligase chain reaction with endonuclease IV correction and contamination control |
ES2141088T3 (es) | 1990-02-16 | 2000-03-16 | Hoffmann La Roche | Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa. |
US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
JPH0515439A (ja) | 1991-07-08 | 1993-01-26 | Matsushita Electric Ind Co Ltd | ジヤー炊飯器の制御回路 |
DE69329800D1 (de) | 1992-04-27 | 2001-02-01 | Dartmouth College | Detektion von gensequenzen in biologischen flüssigkeiten |
AU681082B2 (en) | 1992-05-06 | 1997-08-21 | Gen-Probe Incorporated | Nucleic acid sequence amplification method, composition and kit |
US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
ATE206764T1 (de) * | 1994-07-16 | 2001-10-15 | Roche Diagnostics Gmbh | Verfahren zum sensitiven nachweis von nukleinsäuren |
FR2724934B1 (fr) * | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
WO1996015271A1 (en) * | 1994-11-16 | 1996-05-23 | Abbott Laboratories | Multiplex ligations-dependent amplification |
US20030165908A1 (en) | 1994-12-30 | 2003-09-04 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
DE69535882D1 (de) | 1994-12-30 | 2008-12-18 | Univ Georgetown | Fluoreszenztest zum nachweis der spaltung einer nukleinsäure |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
US5898031A (en) | 1996-06-06 | 1999-04-27 | Isis Pharmaceuticals, Inc. | Oligoribonucleotides for cleaving RNA |
ES2270467T3 (es) | 1996-07-16 | 2007-04-01 | Gen-Probe Incorporated | Metodos para detectar y amplificar secuencias de acido nucleico utilizando oligonucleotidos modificados que tienen una tm especifica de la diana mas elevada. |
US5853990A (en) * | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
DK0866071T3 (da) | 1997-03-20 | 2005-01-17 | Hoffmann La Roche | Modificerede primere |
AU7103098A (en) | 1997-04-16 | 1998-11-11 | Trevigen, Inc. | Detection and mapping of point mutations using partial digestion |
US5846726A (en) | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
AU734636B2 (en) | 1997-07-11 | 2001-06-21 | Xzillion Gmbh & Co. Kg | Characterising nucleic acids |
JPH1132772A (ja) * | 1997-07-24 | 1999-02-09 | Mitsubishi Chem Corp | 耐熱性リボヌクレアーゼh及びそれをコードするdna |
GB9725237D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
WO1999028447A1 (en) | 1997-12-04 | 1999-06-10 | Isis Pharmaceuticals, Inc. | Human rnase h and compositions and uses thereof |
WO2005021776A2 (en) | 2003-08-21 | 2005-03-10 | Isis Pharmaceuticals, Inc. | METHODS OF USING MAMMALIAN RNase H AND COMPOSITIONS THEREOF |
US6248877B1 (en) | 1998-04-07 | 2001-06-19 | Biolink Partners | Solid phase synthesis of organic compounds via phosphitylating reagents |
ATE397092T1 (de) * | 1999-03-10 | 2008-06-15 | Asm Scient Inc | Methode zur direkten sequenzierung von nukleinsäuren |
NZ517121A (en) | 1999-09-13 | 2004-05-28 | Nugen Technologies Inc | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6274353B1 (en) * | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
AU1239901A (en) | 1999-10-29 | 2001-05-14 | Stratagene | Compositions and methods utilizing dna polymerases |
WO2001061015A2 (en) | 2000-02-17 | 2001-08-23 | Qiagen Gmbh | Thermostable chimeric nucleic acid polymerases and uses thereof |
US7033763B2 (en) | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
US6534269B2 (en) | 2000-02-23 | 2003-03-18 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination |
US6596490B2 (en) | 2000-07-14 | 2003-07-22 | Applied Gene Technologies, Inc. | Nucleic acid hairpin probes and uses thereof |
EP1318197B1 (en) | 2000-09-14 | 2008-10-15 | Takara Bio Inc. | Thermotolerant ribonuclease h |
ATE475720T1 (de) | 2000-12-13 | 2010-08-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben |
JP3681729B2 (ja) | 2001-02-15 | 2005-08-10 | タカラバイオ株式会社 | 塩基置換の検出方法 |
US6596489B2 (en) | 2001-03-30 | 2003-07-22 | Applied Gene Technologies | Methods and compositions for analyzing nucleotide sequence mismatches using RNase H |
EP1275735A1 (en) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
US7056671B2 (en) | 2001-08-20 | 2006-06-06 | Takara Bio Inc. | Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide |
CA2409775C (en) * | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
US7629152B2 (en) | 2002-03-01 | 2009-12-08 | Integrated Dna Technologies, Inc. | Methods for amplifying polymeric nucleic acids |
WO2003074724A2 (en) * | 2002-03-01 | 2003-09-12 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
EP2607369B1 (en) * | 2002-08-23 | 2015-09-23 | Illumina Cambridge Limited | Modified nucleotides for polynucleotide sequencing |
CN100343388C (zh) * | 2002-08-30 | 2007-10-17 | 宝生物工程株式会社 | 热稳定的核糖核酸酶h |
CN102268428A (zh) | 2002-11-21 | 2011-12-07 | 震源技术公司 | 使用编码双链启动子一条链的引物的方法 |
US20040175737A1 (en) * | 2002-12-23 | 2004-09-09 | Wyeth | Assay for RNase H activity |
JP2005006587A (ja) * | 2003-06-20 | 2005-01-13 | Takara Bio Inc | 標的核酸の増幅及び/又は検出方法 |
AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
US7439341B2 (en) | 2003-11-14 | 2008-10-21 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
KR101041106B1 (ko) | 2003-11-25 | 2011-06-13 | 한면기 | 핵산과 단백질의 신규한 실시간 탐지방법 |
US7344834B2 (en) | 2003-12-08 | 2008-03-18 | Cytyc Corporation | Method for DNA amplification using DNA blocking probes |
JP4249186B2 (ja) * | 2003-12-10 | 2009-04-02 | タカラバイオ株式会社 | 核酸の増幅方法 |
CN101426908B (zh) * | 2004-06-09 | 2014-06-11 | 纽星实验室 | 可逆性修饰的热稳定酶组合物及其制备方法和使用方法 |
CA2588865A1 (en) | 2004-11-23 | 2006-06-01 | Xiao Bing Wang | Detection of nucleic acid variation by cleavage-amplification method |
WO2006070666A1 (ja) * | 2004-12-28 | 2006-07-06 | Takara Bio Inc. | 遺伝子多型の同時検出方法 |
WO2006074233A2 (en) * | 2005-01-06 | 2006-07-13 | Applera Corporation | Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification |
WO2006081426A2 (en) * | 2005-01-28 | 2006-08-03 | Applera Corporation | Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template |
EP2857523A1 (en) | 2005-02-01 | 2015-04-08 | Applied Biosystems, LLC | Method for identifying a sequence in a polynucleotide |
US20060211024A1 (en) | 2005-03-10 | 2006-09-21 | Gwc Technologies Incorporated | Methods for analysis of a nucleic acid sample |
US20090068643A1 (en) | 2005-11-23 | 2009-03-12 | Integrated Dna Technologies, Inc. | Dual Function Primers for Amplifying DNA and Methods of Use |
EP1954706B1 (en) * | 2005-11-30 | 2012-03-28 | Epicentre Technologies Corporation | Method using reversibly blocked tagging oligonucleotides |
WO2007103558A2 (en) | 2006-03-09 | 2007-09-13 | The Regents Of The University Of California | Hybrid energy transfer for nucleic acid detection |
US20070218490A1 (en) | 2006-03-15 | 2007-09-20 | Integrated Dna Technologies, Inc. | Nucleic acid monomers with 2'-chemical moieties |
AU2007268075C1 (en) | 2006-06-01 | 2013-03-07 | Trilink Biotechnologies | Chemically modified oligonucleotide primers for nucleic acid amplification |
GB0611444D0 (en) * | 2006-06-09 | 2006-07-19 | Medical Res Council Technology | Rnase H2 complex and genes therefor |
US20080009007A1 (en) * | 2006-06-16 | 2008-01-10 | Pacific Biosciences Of California, Inc. | Controlled initiation of primer extension |
JP2008072427A (ja) | 2006-09-14 | 2008-03-27 | Konica Minolta Business Technologies Inc | 画像形成装置及びクライアント/サーバ型情報処理システム並びに情報処理方法 |
EP1921156A1 (en) | 2006-11-10 | 2008-05-14 | bioMerieux B.V. | Improved multiplex nucleic acid amplification using blocked primers |
WO2009135093A2 (en) | 2008-04-30 | 2009-11-05 | Integrated Dna Technologies, Inc. | Rnase-h-based assays utilizing modified rna monomers |
US20110117559A1 (en) | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
US8618253B2 (en) | 2010-05-25 | 2013-12-31 | Samsung Techwin Co., Ltd. | Modified RNAse H and detection of nucleic acid amplification |
US20120052501A1 (en) | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Kit for detecting htlv strains and use thereof |
US20120052495A1 (en) | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria monocytogenes and use thereof |
-
2009
- 2009-04-30 WO PCT/US2009/042454 patent/WO2009135093A2/en active Application Filing
- 2009-04-30 AU AU2009242546A patent/AU2009242546B2/en active Active
- 2009-04-30 EP EP13173388.3A patent/EP2644707B1/en active Active
- 2009-04-30 EP EP16198184.0A patent/EP3150727B1/en active Active
- 2009-04-30 JP JP2011507669A patent/JP5539325B2/ja active Active
- 2009-04-30 US US12/433,896 patent/US20090325169A1/en not_active Abandoned
- 2009-04-30 CA CA2722541A patent/CA2722541C/en active Active
- 2009-04-30 EP EP13173389.1A patent/EP2644708B1/en not_active Not-in-force
- 2009-04-30 EP EP13173391.7A patent/EP2644710B1/en active Active
- 2009-04-30 DK DK13173388.3T patent/DK2644707T3/da active
- 2009-04-30 DK DK09739895.2T patent/DK2279263T3/da active
- 2009-04-30 EP EP13173390.9A patent/EP2644709B1/en active Active
- 2009-04-30 EP EP09739895.2A patent/EP2279263B1/en active Active
-
2013
- 2013-08-05 US US13/959,637 patent/US9644198B2/en active Active
- 2013-09-06 JP JP2013185104A patent/JP2014014371A/ja active Pending
-
2015
- 2015-06-09 JP JP2015116458A patent/JP2015221036A/ja not_active Ceased
-
2016
- 2016-12-20 JP JP2016246235A patent/JP6453296B2/ja active Active
- 2016-12-20 JP JP2016246236A patent/JP6276835B2/ja not_active Expired - Fee Related
-
2017
- 2017-04-21 US US15/493,781 patent/US20170355978A1/en not_active Abandoned
-
2018
- 2018-03-05 JP JP2018038239A patent/JP6510695B2/ja active Active
- 2018-07-13 US US16/034,775 patent/US20190002865A1/en not_active Abandoned
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK2644707T3 (da) | RNase-H-baserede assays, som anvender modificerede RNA-monomere. | |
US8911948B2 (en) | RNase H-based assays utilizing modified RNA monomers | |
US9434988B2 (en) | RNase H-based assays utilizing modified RNA monomers | |
US10227641B2 (en) | RNase H-based assays utilizing modified RNA monomers | |
AU2013381709A1 (en) | RNase H-based assays utilizing modified RNA monomers | |
CA2949315A1 (en) | Rnase h-based assays utilizing modified rna monomers | |
AU2013203629A1 (en) | RNase-H-Based Assays Utilizing Modified RNA Monomers |