DK2398912T3 - Conservation of cell-free nucleic acids - Google Patents
Conservation of cell-free nucleic acids Download PDFInfo
- Publication number
- DK2398912T3 DK2398912T3 DK10704474.5T DK10704474T DK2398912T3 DK 2398912 T3 DK2398912 T3 DK 2398912T3 DK 10704474 T DK10704474 T DK 10704474T DK 2398912 T3 DK2398912 T3 DK 2398912T3
- Authority
- DK
- Denmark
- Prior art keywords
- cell
- blood
- blood sample
- nucleic acids
- free
- Prior art date
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C09K15/00—Anti-oxidant compositions; Compositions inhibiting chemical change
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- C09K15/20—Anti-oxidant compositions; Compositions inhibiting chemical change containing organic compounds containing nitrogen and oxygen
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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| US10083273B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US10081839B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US8532930B2 (en) | 2005-11-26 | 2013-09-10 | Natera, Inc. | Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals |
| US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US20110178719A1 (en) | 2008-08-04 | 2011-07-21 | Gene Security Network, Inc. | Methods for Allele Calling and Ploidy Calling |
| US20100167271A1 (en) * | 2008-12-30 | 2010-07-01 | Streck, Inc. | Method for screening blood using a preservative that may be in a substantially solid state form |
| US11634747B2 (en) | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| DK3290530T3 (da) | 2009-02-18 | 2020-12-07 | Streck Inc | Konservering af cellefrie nukleinsyrer |
| EP2473638B1 (en) | 2009-09-30 | 2017-08-09 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| EP3103883A1 (en) * | 2009-11-09 | 2016-12-14 | Streck, Inc. | Stabilization of rna in and extracting from intact cells within a blood sample |
| WO2011113043A2 (en) * | 2010-03-12 | 2011-09-15 | University Of South Florida | Sample storage for molecular and immunological detection |
| US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US8825412B2 (en) | 2010-05-18 | 2014-09-02 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US9040255B2 (en) | 2010-09-23 | 2015-05-26 | Biocept, Inc. | Use of diazolidinyl urea for anti-clumping of biological samples |
| CA2821906C (en) | 2010-12-22 | 2020-08-25 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| CA2824387C (en) | 2011-02-09 | 2019-09-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| WO2012151391A2 (en) | 2011-05-04 | 2012-11-08 | Streck, Inc. | Inactivated virus compositions and methods of preparing such compositions |
| BR112013032629A2 (pt) | 2011-06-19 | 2017-08-01 | Abogen Inc | dispositivos, soluções e métodos para coletamento de amostra |
| US10676780B2 (en) | 2011-09-26 | 2020-06-09 | Qiagen Gmbh | Stabilisation and isolation of extracellular nucleic acids |
| US20140227688A1 (en) | 2011-09-26 | 2014-08-14 | Qiagen Gmbh | Stabilisation and isolation of extracellular nucleic acids |
| CN113388606A (zh) | 2011-09-26 | 2021-09-14 | 凯杰有限公司 | 用于分离细胞外核酸的快速方法 |
| US11021733B2 (en) | 2011-09-26 | 2021-06-01 | Qiagen Gmbh | Stabilization and isolation of extracellular nucleic acids |
| WO2013074927A1 (en) * | 2011-11-16 | 2013-05-23 | University Of Georgia Research Foundation, Inc. | Method for isolating total rna from cells |
| WO2013123030A2 (en) * | 2012-02-13 | 2013-08-22 | Streck, Inc. | Blood collection device for improved nucleic acid regulation |
| DE102012101896A1 (de) * | 2012-03-06 | 2013-09-12 | Roberto Gerigk | Formalinfreie Fixierungsmittel für histologische Färbungen von Gewebeproben |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| EP2888354B1 (en) * | 2012-08-21 | 2020-04-01 | Qiagen GmbH | Virus particle stabilisation and method for isolating viral nucleic acids |
| CN104755628B (zh) | 2012-09-25 | 2019-03-01 | 凯杰有限公司 | 生物样品的稳定化 |
| US20140199681A1 (en) * | 2013-01-14 | 2014-07-17 | Streck, Inc. | Blood collection device for stabilizing cell-free rna in blood during sample shipping and storage |
| AU2014233373B2 (en) | 2013-03-15 | 2019-10-24 | Verinata Health, Inc. | Generating cell-free DNA libraries directly from blood |
| EP2976424B1 (en) * | 2013-03-18 | 2018-10-03 | Qiagen GmbH | Stabilisation of biological samples |
| CN105121643A (zh) | 2013-03-18 | 2015-12-02 | 凯杰有限公司 | 细胞外核酸的稳定化和分离 |
| CA2917912C (en) | 2013-07-24 | 2019-09-17 | Streck, Inc. | Compositions and methods for stabilizing circulating tumor cells |
| US9644232B2 (en) | 2013-07-26 | 2017-05-09 | General Electric Company | Method and device for collection and amplification of circulating nucleic acids |
| US9499870B2 (en) | 2013-09-27 | 2016-11-22 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| US10262755B2 (en) | 2014-04-21 | 2019-04-16 | Natera, Inc. | Detecting cancer mutations and aneuploidy in chromosomal segments |
| US10577655B2 (en) | 2013-09-27 | 2020-03-03 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| US20170009288A1 (en) | 2014-02-03 | 2017-01-12 | Thermo Fisher Scientific Baltics Uab | Method for controlled dna fragmentation |
| CA2940692C (en) | 2014-03-18 | 2025-07-08 | Qiagen Gmbh | STABILIZATION AND ISOLATION OF EXTRACELLULAR NUCLEIC ACIDS |
| US12492429B2 (en) | 2014-04-21 | 2025-12-09 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| EP3957749A1 (en) | 2014-04-21 | 2022-02-23 | Natera, Inc. | Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| WO2015191632A1 (en) | 2014-06-10 | 2015-12-17 | Biomatrica, Inc. | Stabilization of thrombocytes at ambient temperatures |
| EP3155395B1 (en) * | 2014-06-10 | 2021-06-02 | Biomatrica, INC. | Methods and compositions for stabilization of nucleic acids in a blood sample at ambient temperatures |
| US10604751B2 (en) | 2014-07-02 | 2020-03-31 | Siemens Healthcare Disgnostics Inc. | Selective nucleic acid separation |
| EP3656875B1 (en) | 2014-07-18 | 2021-09-22 | Illumina, Inc. | Non-invasive prenatal diagnosis |
| US11773447B2 (en) | 2014-11-14 | 2023-10-03 | Nanthealth Labs, Inc. | Use of circulating cell-free RNA for diagnosis and/or monitoring cancer |
| US11168351B2 (en) | 2015-03-05 | 2021-11-09 | Streck, Inc. | Stabilization of nucleic acids in urine |
| US11479812B2 (en) | 2015-05-11 | 2022-10-25 | Natera, Inc. | Methods and compositions for determining ploidy |
| EP3307886B1 (en) | 2015-06-10 | 2021-03-24 | Qiagen GmbH | Method for isolating extracellular nucleic acids using anion exchange particles |
| CN108473932B (zh) | 2015-09-09 | 2022-07-15 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
| US20170145475A1 (en) | 2015-11-20 | 2017-05-25 | Streck, Inc. | Single spin process for blood plasma separation and plasma composition including preservative |
| JP7166169B2 (ja) | 2015-11-20 | 2022-11-07 | キアゲン ゲーエムベーハー | 細胞外核酸の滅菌のための滅菌組成物を調製する方法 |
| WO2017100212A1 (en) | 2015-12-08 | 2017-06-15 | Biomatrica, Inc. | Reduction of erythrocyte sedimentation rate |
| DE102016102346A1 (de) * | 2016-02-10 | 2017-08-10 | Biosepar Gesellschaft für Medizin- und Labortechnik mbH | Fixativum |
| JP7280044B2 (ja) | 2016-04-15 | 2023-05-23 | ナテラ， インコーポレイテッド | 肺癌の検出方法 |
| WO2017201612A1 (en) | 2016-05-27 | 2017-11-30 | Norgen Biotek Corp. | Preservation of cell-free nucleic acids in biological samples |
| US11506655B2 (en) | 2016-07-29 | 2022-11-22 | Streck, Inc. | Suspension composition for hematology analysis control |
| CA3033650A1 (en) | 2016-08-17 | 2018-02-22 | The Regents Of The University Of California | A novel immunoprobe-based method to assess organ injury status through a biofluid-based cell-free dna (cfdna) assay |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| US20210072255A1 (en) | 2016-12-16 | 2021-03-11 | The Brigham And Women's Hospital, Inc. | System and method for protein corona sensor array for early detection of diseases |
| US10894976B2 (en) | 2017-02-21 | 2021-01-19 | Natera, Inc. | Compositions, methods, and kits for isolating nucleic acids |
| US11795192B2 (en) | 2017-03-10 | 2023-10-24 | Lakewood Amedex, Inc. | Antimicrobial compounds, compositions, and uses thereof |
| KR20190129094A (ko) * | 2017-03-17 | 2019-11-19 | 난토믹스, 엘엘씨 | cfRNA에 대한 액체 생검(LIQUID BIOPSY FOR cfRNA) |
| CA3062985A1 (en) * | 2017-05-16 | 2018-11-22 | The Chinese University Of Hong Kong | Integrative single-cell and cell-free plasma rna analysis |
| WO2018222709A2 (en) | 2017-06-01 | 2018-12-06 | Nantomics, Llc | Dna stabilization of rna |
| RU2764245C2 (ru) * | 2017-08-02 | 2022-01-14 | Зарштедт Аг Унд Ко. Кг | Способ и состав для стабилизации нуклеиновых кислот, свободных от клеток, и клеток |
| EP4427588A3 (en) * | 2017-10-19 | 2024-10-16 | Streck LLC | Compositions for hemolysis and coagulation regulation and stabilization of extracellular vesicles |
| WO2019094363A2 (en) * | 2017-11-07 | 2019-05-16 | Nanthealth Labs, Inc. | Targeted cell free nucleic acid analysis |
| WO2019118926A1 (en) | 2017-12-14 | 2019-06-20 | Tai Diagnostics, Inc. | Assessing graft suitability for transplantation |
| CN108085391B (zh) * | 2017-12-28 | 2021-01-19 | 广州市金圻睿生物科技有限责任公司 | 一种可稳定保存的肺癌融合基因检测试剂盒的rna质控品 |
| CN108103054B (zh) * | 2017-12-28 | 2020-10-02 | 广州市金圻睿生物科技有限责任公司 | 一种rna保存液及其用途 |
| CN108220417B (zh) * | 2017-12-28 | 2021-08-13 | 广州市金圻睿生物科技有限责任公司 | 可稳定保存的染色体非整倍体检测试剂盒的质控品及应用 |
| CN108220478B (zh) * | 2017-12-28 | 2021-08-17 | 广州市金圻睿生物科技有限责任公司 | 可稳定保存的丙型肝炎病毒基因检测试剂盒的病毒质控品 |
| CN108048529B (zh) * | 2017-12-28 | 2021-01-19 | 广州市金圻睿生物科技有限责任公司 | 一种可稳定保存的肺癌甲基化基因检测试剂盒的质控品及应用 |
| US12398389B2 (en) | 2018-02-15 | 2025-08-26 | Natera, Inc. | Methods for isolating nucleic acids with size selection |
| US12024738B2 (en) | 2018-04-14 | 2024-07-02 | Natera, Inc. | Methods for cancer detection and monitoring |
| WO2019210306A1 (en) * | 2018-04-27 | 2019-10-31 | Momenta Pharmaceuticals , Inc. | Blood preparation |
| CN108893524A (zh) * | 2018-06-04 | 2018-11-27 | 北京启衡星生物科技有限公司 | 血浆中游离dna的保护剂 |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| EP3830296A1 (en) | 2018-07-27 | 2021-06-09 | F. Hoffmann-La Roche AG | Method of monitoring effectiveness of immunotherapy of cancer patients |
| EP3847653A2 (en) | 2018-09-07 | 2021-07-14 | Illumina, Inc. | A method to determine if a circulating fetal cell isolated from a pregnant mother is from either the current or a historical pregnancy |
| WO2020096631A2 (en) | 2018-11-07 | 2020-05-14 | Seer, Inc. | Compositions, methods and systems for protein corona analysis and uses thereof |
| WO2020140035A1 (en) | 2018-12-27 | 2020-07-02 | Streck, Inc. | Methods of preparing samples for proteomic analysis |
| CN111378708B (zh) * | 2018-12-28 | 2022-07-19 | 康码（上海）生物科技有限公司 | 一种体外无细胞蛋白合成体系及其应用 |
| AU2020247907B2 (en) | 2019-03-26 | 2025-12-11 | Seer, Inc. | Compositions, methods and systems for protein corona analysis from biofluids and uses thereof |
| WO2020247263A1 (en) | 2019-06-06 | 2020-12-10 | Natera, Inc. | Methods for detecting immune cell dna and monitoring immune system |
| CN110747193B (zh) * | 2019-09-25 | 2021-08-31 | 广州市达瑞生物技术股份有限公司 | 一种基于二代测序平台的肺癌多基因联合检测试剂盒质控品 |
| CN111117999A (zh) * | 2019-12-18 | 2020-05-08 | 上海兰卫医学检验所股份有限公司 | 一种适用于液态活检技术的血浆exRNA提取保存方法 |
| AU2021225854A1 (en) * | 2020-02-24 | 2022-10-13 | The Board Of Trustees Of The Leland Stanford Junior University | Systems and methods for protecting nucleic-acid molecules |
| WO2022006260A1 (en) | 2020-07-01 | 2022-01-06 | Streck, Inc. | Methods of preparing samples for proteomic analysis |
| EP4179066A4 (en) * | 2020-07-10 | 2024-06-19 | Acutis Diagnostics, Inc. | Methods and compositions for isolation and rapid detection of micro-organisms from blood and bodily fluids |
| CN116390811A (zh) | 2020-08-13 | 2023-07-04 | 斯特雷克股份有限公司 | 样本采集容器、工艺和所采集样本 |
| CN112251358A (zh) * | 2020-10-23 | 2021-01-22 | 瑞因迈拓科技(广州)有限公司 | 一种用于病原微生物检测体液标本的保存剂及其应用 |
| CN112501159A (zh) * | 2020-12-04 | 2021-03-16 | 麦凯(上海)生物科技有限公司 | 一种通用型游离核酸保护剂 |
| WO2022133159A1 (en) | 2020-12-17 | 2022-06-23 | Nephrosant, Inc. | Kits for stabilization of urine samples |
| WO2022150283A1 (en) * | 2021-01-05 | 2022-07-14 | Nuclease Probe Technologies, Inc. | Sars-cov-2 detection |
| CN112760360B (zh) * | 2021-03-22 | 2023-12-19 | 洛阳赛奥生物工程技术有限公司 | 一种rna保护剂及其应用 |
| KR20240046683A (ko) * | 2021-08-25 | 2024-04-09 | 세키스이 메디칼 가부시키가이샤 | 세포 함유액용 보존액 및 세포 함유액용 보존 용기 |
| AU2023298557A1 (en) * | 2022-06-29 | 2025-01-16 | Life Technologies Corporation | Systems and methods for analyte detection from multiplexed assays |
| KR102699668B1 (ko) * | 2022-07-22 | 2024-08-28 | 주식회사 어큐진 | 분변 rna 보존액 |
| EP4356731A1 (en) * | 2022-10-19 | 2024-04-24 | Olga Alekseevna Gra | Medium and device for collecting, storing and transporting biological samples, as well as their use for the stabilization of extracellular nucleic acids |
| GB202303562D0 (en) | 2023-03-10 | 2023-04-26 | Belgian Volition Sprl | Sample collection for liquid biopsy |
| WO2024192232A1 (en) | 2023-03-14 | 2024-09-19 | Streck Llc | Compositions and uses thereof for stabilization of blood sample components |
| WO2025167593A1 (zh) * | 2024-02-06 | 2025-08-14 | 上海细胞治疗集团股份有限公司 | 生物样本的存储保护剂、采集卡、存储和检测方法 |
Family Cites Families (192)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1432249A (en) | 1922-02-06 | 1922-10-17 | Howard S Borden | Article of manufacture |
| US1922799A (en) * | 1932-12-16 | 1933-08-15 | George E Gaus | Method for affixing identification tags |
| US2250666A (en) * | 1938-10-31 | 1941-07-29 | Godefroy Mfg Company | Combined label, cap loosener, and auxiliary container |
| US2690624A (en) | 1949-09-15 | 1954-10-05 | Ollie W Phillips | Sign |
| US2930570A (en) * | 1959-01-30 | 1960-03-29 | Herbert R Leedy | Pinch clamp |
| US3867521A (en) | 1970-08-26 | 1975-02-18 | Scherer Corp R P | Method for absorption of drugs |
| US3781120A (en) | 1970-09-14 | 1973-12-25 | Technicon Instr | Self-locating sample receptacle having integral identification label |
| US3874384A (en) | 1971-11-01 | 1975-04-01 | American Hospital Supply Corp | Improved blood storage unit and method of storing blood |
| US3872730A (en) | 1972-03-10 | 1975-03-25 | Becton Dickinson Co | Sampling apparatus |
| US3879295A (en) | 1973-08-17 | 1975-04-22 | Eastman Kodak Co | Vacutainer with positive separation barrier |
| US3973913A (en) | 1976-01-29 | 1976-08-10 | Louderback Allan Lee | Blood control standard |
| US3994085A (en) | 1976-03-29 | 1976-11-30 | Groselak Robert E | Baggage tag |
| US4043453A (en) * | 1976-05-11 | 1977-08-23 | The Wright Tool & Forge Company | Holder and display device for wrench sockets |
| US4318090A (en) * | 1980-10-27 | 1982-03-02 | Sensormatic Electronics Corporation | Apparatus for deactivating a surveillance tag |
| US4513522A (en) * | 1982-09-16 | 1985-04-30 | Selenke William M | Label with particular application to laboratory specimen container identification |
| US4515890A (en) | 1982-11-08 | 1985-05-07 | Abbott Laboratories | Immunoassay of terminal deoxynucleotidyl transferase |
| DE8407967U1 (de) * | 1984-03-15 | 1984-06-07 | Idento - Gesellschaft für industrielle Kennzeichnung mbH, 6074 Rödermark | Beschriftbarer Kabelmarkierungsstreifen |
| US4584219A (en) * | 1984-11-09 | 1986-04-22 | Baartmans Hans R | Web of labels |
| US4675159A (en) | 1985-09-29 | 1987-06-23 | Al Sioufi Habib | Method and device for disinfecting biological fluids and container for same |
| US5110908A (en) | 1986-12-31 | 1992-05-05 | Praxis Biologics, Inc. | Haemophilus influenzae peptides and proteins |
| US4884827A (en) | 1988-01-22 | 1989-12-05 | Norfolk Scientific Inc. | Partially transparent label |
| US4921277A (en) * | 1988-10-24 | 1990-05-01 | Academy Of Applied Science, Inc. | Method of labeling needle syringes and medication vials and novel labels therefor |
| HU201865B (en) | 1989-04-28 | 1991-01-28 | Pecsi Dohanygyar | Tobacco-smoke filter of high efficiency |
| US5000484A (en) * | 1989-08-30 | 1991-03-19 | Phelan James C | Identification and monitoring system for surgical specimens |
| US5153117A (en) * | 1990-03-27 | 1992-10-06 | Genetype A.G. | Fetal cell recovery method |
| JP2540649B2 (ja) * | 1990-04-27 | 1996-10-09 | テルモ株式会社 | 採血管 |
| US5250438A (en) | 1990-05-09 | 1993-10-05 | Streck Laboratories, Inc. | Method for differential determination of white blood cells using diazolidinyl urea to stabilize white blood cells |
| US5135125A (en) * | 1991-02-15 | 1992-08-04 | Tapecon, Inc. | Hanging label |
| US5849517A (en) | 1991-05-08 | 1998-12-15 | Streck Laboratories, Inc. | Method and composition for preserving antigens and nucleic acids and process for utilizing cytological material produced by same |
| US5196182A (en) | 1991-05-08 | 1993-03-23 | Streck Laboratories, Inc. | Tissue fixative |
| US5260048A (en) | 1991-05-08 | 1993-11-09 | Streck Laboratories, Inc. | Tissue fixative solution and method |
| US5459073A (en) | 1991-05-08 | 1995-10-17 | Streck Laboratories, Inc. | Method and composition for preserving antigens and process for utilizing cytological material produced by same |
| US5460797A (en) | 1991-05-08 | 1995-10-24 | Streck Laboratories, Inc. | Method for fixing tissues and cells for analysis using oxazolidine compounds |
| US5459253A (en) | 1991-07-19 | 1995-10-17 | Pharmacia P-L Biochemicals Inc. | M-RNA purification |
| US5343647A (en) * | 1991-09-03 | 1994-09-06 | Moore Business Forms, Inc. | Pressure sensitive pricing tag/label |
| US5998483A (en) | 1991-09-20 | 1999-12-07 | Camiener; Gerald W. | Glyoxal-containing preservative compositions |
| JPH07507266A (ja) | 1991-09-20 | 1995-08-10 | カミナー，ジェラルド・ダブリュー | アルデヒド安定化溶液を用いる方法および組成物 |
| DE4134231A1 (de) | 1991-10-16 | 1993-04-22 | Fix Gmbh | Gepaeckstreifenanhaenger fuer einzelbeschriftung |
| US5257633A (en) | 1992-06-23 | 1993-11-02 | Becton, Dickinson And Company | Surface modified blood collection tubes |
| EP0662152A1 (en) | 1992-07-17 | 1995-07-12 | Aprogenex, Inc. | Enriching and identyfying fetal cells in maternal blood for in situ hybridization |
| US5629147A (en) * | 1992-07-17 | 1997-05-13 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
| GB9223035D0 (en) | 1992-11-03 | 1992-12-16 | Kiessling Cooper Ann A | Preservation of peripheral blood & semen in fixatives that inactivate human pathogens |
| US5688516A (en) | 1992-11-12 | 1997-11-18 | Board Of Regents, The University Of Texas System | Non-glycopeptide antimicrobial agents in combination with an anticoagulant, an antithrombotic or a chelating agent, and their uses in, for example, the preparation of medical devices |
| AU669754B2 (en) | 1992-12-18 | 1996-06-20 | Becton Dickinson & Company | Barrier coating |
| US5457024A (en) | 1993-01-22 | 1995-10-10 | Aprogenex, Inc. | Isolation of fetal erythrocytes |
| AU6230594A (en) | 1993-02-01 | 1994-08-29 | University Of Iowa Research Foundation, The | Quartenary amine surfactants and methods of using same in isolation of rna |
| NZ271318A (en) * | 1993-07-05 | 1998-08-26 | North Gen Hospital Nhs Trust | Leukocyte cell preparations stabilised with heavy metal compounds |
| US6043032A (en) * | 1993-09-22 | 2000-03-28 | Tosoh Corporation | Method of extracting nucleic acids and method of detecting specified nucleic acid sequences |
| WO1995026417A1 (en) | 1994-03-29 | 1995-10-05 | Genzyme Corporation | Culture and isolation of fetal cells from maternal peripheral blood |
| ES2122589T3 (es) * | 1994-04-05 | 1998-12-16 | North Gen Hospital Nhs Trust | Preparacion y estabilizacion de suspensiones celulares. |
| US5501954A (en) * | 1994-06-13 | 1996-03-26 | Genzyme Corporation | Method of detecting cellular material |
| US5468022A (en) | 1994-06-14 | 1995-11-21 | Massachusetts Institute Of Technology | Sample tube identification flag |
| FR2722506B1 (fr) * | 1994-07-13 | 1996-08-14 | Rhone Poulenc Rorer Sa | Composition contenant des acides nucleiques, preparation et utilisations |
| US5512343A (en) * | 1994-08-01 | 1996-04-30 | Diagraph Corporation | Label assembly |
| US5540358A (en) * | 1994-12-19 | 1996-07-30 | The Procter And Gamble Company | Flexible planar gusseted package for dispensing a product through a fitment |
| US5490658A (en) * | 1995-03-02 | 1996-02-13 | Avery Dennison Corporation | Label hangers for intravenous bottles |
| US5560657A (en) | 1995-03-08 | 1996-10-01 | Morgan; Brian R. | Tamper-indicating label |
| USD382343S (en) * | 1995-06-06 | 1997-08-12 | Home Access Health Corporation | Filmstrip for enhancing blood flow |
| US6527957B1 (en) * | 1995-08-09 | 2003-03-04 | Baxter International Inc. | Methods for separating, collecting and storing red blood cells |
| US5817519A (en) | 1995-12-28 | 1998-10-06 | Bayer Corporation | Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
| US6630301B1 (en) | 1997-03-14 | 2003-10-07 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum |
| AU2324997A (en) | 1996-03-15 | 1997-10-01 | Penn State Research Foundation, The | Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays |
| ES2347854T5 (es) | 1996-03-26 | 2014-07-24 | Michael S. Kopreski | Método que permite el uso de RNA extracelular extraído de plasma o suero para detectar, monitorizar o evaluar un cáncer |
| US6759217B2 (en) | 1996-03-26 | 2004-07-06 | Oncomedx, Inc. | Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer |
| US6072086A (en) | 1996-04-12 | 2000-06-06 | Intergen Company | Method and composition for controlling formaldehyde fixation by delayed quenching |
| GB2313288B (en) * | 1996-05-24 | 2000-07-12 | North Gen Hospital Nhs Trust | Specimen collection fluid |
| IT1294964B1 (it) | 1996-07-12 | 1999-04-23 | Domenico Valerio | Isolamento e cultura di cellule fetali dal sangue periferico materno |
| US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| US5731156A (en) * | 1996-10-21 | 1998-03-24 | Applied Imaging, Inc. | Use of anti-embryonic hemoglobin antibodies to identify fetal cells |
| US6190609B1 (en) * | 1996-11-19 | 2001-02-20 | Baxter International Inc. | Methods and apparatus for inactivating contaminants in biological fluid |
| US5783093A (en) * | 1997-01-02 | 1998-07-21 | Haemonetics Corporation | Blood cell concentrates using a single solution for anticoagulation and preservation |
| CA2278245A1 (en) * | 1997-01-21 | 1998-07-23 | The American National Red Cross | Intracellular and extracellular decontamination of whole blood and blood components by amphiphilic phenothiazin-5-ium dyes plus light |
| US6125563A (en) | 1997-02-14 | 2000-10-03 | Girerd; Philippe H. | Container label with handle flap |
| GB9704444D0 (en) * | 1997-03-04 | 1997-04-23 | Isis Innovation | Non-invasive prenatal diagnosis |
| US20010051341A1 (en) | 1997-03-04 | 2001-12-13 | Isis Innovation Limited | Non-invasive prenatal diagnosis |
| DE69818650T2 (de) * | 1997-03-25 | 2004-08-12 | Immunivest Corp., Wilmington | Gerät und methoden zum einfnag und zur analyse von partikel-einheiten |
| SE514350C2 (sv) * | 1997-04-07 | 2001-02-12 | Resemino System Ab | Metod för affischering samt för metoden anpassat system för affischering |
| US5939259A (en) * | 1997-04-09 | 1999-08-17 | Schleicher & Schuell, Inc. | Methods and devices for collecting and storing clinical samples for genetic analysis |
| US5906744A (en) | 1997-04-30 | 1999-05-25 | Becton Dickinson And Company | Tube for preparing a plasma specimen for diagnostic assays and method of making thereof |
| US5976014A (en) | 1997-05-28 | 1999-11-02 | Moore U.S.A., Inc. | Integrity seal form/label combination |
| EP0920539B9 (en) * | 1997-05-30 | 2006-08-02 | Xenomics | Methods for detection of nucleic acid sequences in urine |
| US6083731A (en) | 1997-06-24 | 2000-07-04 | Washington State University Research Foundation | Recombinant materials and methods for the production of limonene hydroxylases |
| US6218531B1 (en) * | 1997-06-25 | 2001-04-17 | Promega Corporation | Method of isolating RNA |
| US5962234A (en) | 1997-10-20 | 1999-10-05 | Applied Imaging Corporation | Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells |
| CA2260402C (en) | 1998-01-22 | 2001-10-23 | Pierre Boisvert | Display card |
| US6210889B1 (en) * | 1998-01-28 | 2001-04-03 | The Universite Laval | Method for enrichment of fetal cells from maternal blood and use of same in determination of fetal sex and detection of chromosomal abnormalities |
| BR9907852A (pt) | 1998-02-12 | 2000-10-24 | Immunivest Corp | Processos para detectar e enumerar células raras e cancerosas em uma população celular mista, para diagnosticar câncer de estágio precoce em um paciente de teste, para determinar a probabilidade de recorrência de câncer em um paciente humano anteriormente tratado de câncer, para distinguir um carcinoma confinado ao órgão de um carcinoma com propriedades metastáticas, para acompanhar a situação de remissão em um paciente humano com câncer que passa pelo tratamento de terapia contra o câncer e para aumentar quantidades de células epiteliais circulantes em uma amostra de sangue, partìcula magnética revestida, composição, conjuntos de teste para avaliar uma amostra de paciente quanto a presença de células raras circulantes, quanto a presença de células de tumor circulantes, quanto a presença de células de câncer de mama circulantes, quanto a presença de células de câncer de próstata circulantes, quanto a presença de células de câncer de cólon circulantes, quanto a presença de células de câncer de bexiga circulantes e para monitorar um paciente quanto a recorrência de câncer, e, fração de sangue periférico enriquecido quanto a células neoplásticas circulantes |
| US20010018192A1 (en) * | 1998-02-12 | 2001-08-30 | Terstappen Leon W.M.M. | Labeled cells for use as an internal functional control in rare cell detection assays |
| US6177163B1 (en) * | 1998-06-22 | 2001-01-23 | Tricor Direct, Inc. | Markable repositionable adhesive sheet dispensing roll for use in an industrial setting |
| US6204375B1 (en) | 1998-07-31 | 2001-03-20 | Ambion, Inc. | Methods and reagents for preserving RNA in cell and tissue samples |
| US20060204989A1 (en) * | 1998-09-22 | 2006-09-14 | Kopreski Michael S | Comparative analysis of extracellular RNA species |
| US20090233276A1 (en) | 1998-09-22 | 2009-09-17 | Oncomedx, Inc. | Method Enabling the Use of Extracellular Ribonucleic Acid (RNA) Extracted from Plasma or Serum to Detect, Monitor or Evaluate Cancer or Premalignant Conditions |
| US7683035B1 (en) * | 1999-02-23 | 2010-03-23 | Qiagen, Gmbh | Method of stabilizing and/or isolating nucleic acids |
| US6077235A (en) | 1999-02-23 | 2000-06-20 | Becton, Dickinson And Company | Blood collection assembly and method therefor |
| WO2000075647A1 (en) | 1999-06-04 | 2000-12-14 | Bioseparations, Inc. | System and method for prenatal diagnostic screening |
| AU5620600A (en) | 1999-06-16 | 2001-01-02 | University Of Cincinnati, The | Agent and process for isolation of extra-chromosomal nucleic acids |
| DE19928820A1 (de) * | 1999-06-17 | 2000-12-21 | Ulrich Groth | Fixiermittel für Zellen und Gewebe von Lebewesen |
| AU6498600A (en) * | 1999-07-29 | 2001-02-19 | Baxter International Inc. | Sampling tube holder for blood sampling system |
| WO2001021830A1 (en) | 1999-09-24 | 2001-03-29 | Ambion, Inc. | Nuclease inhibitor cocktail |
| US20010049895A1 (en) | 2000-01-06 | 2001-12-13 | Burke Doyle G. | Promotional hanger |
| US6337189B1 (en) * | 2000-02-08 | 2002-01-08 | Streck Laboratories, Inc. | Fixative system, method and composition for biological testing |
| DE10006662A1 (de) | 2000-02-15 | 2001-08-23 | Antigen Produktions Gmbh | Gefäß zur Nukleinsäureanalytik |
| GB0009179D0 (en) | 2000-04-13 | 2000-05-31 | Imp College Innovations Ltd | Non-invasive prenatal diagnosis |
| GB0009784D0 (en) | 2000-04-20 | 2000-06-07 | Simeg Limited | Methods for clinical diagnosis |
| US20020045196A1 (en) * | 2000-05-12 | 2002-04-18 | Walt Mahoney | Methods of isolating trophoblast cells from maternal blood |
| CA2411077C (en) * | 2000-06-08 | 2013-02-05 | Stacy R. Kaufman | Verification of prescription information and warning label |
| AU1548802A (en) | 2000-06-21 | 2002-01-02 | Digene Corp | Universal collection medium |
| DE60131986T2 (de) * | 2000-08-10 | 2008-08-28 | Baxa Corp., Englewood | Artikel zum handhaben, etikettieren, füllen und verschliessen von spritzen |
| ES2306731T3 (es) | 2000-10-17 | 2008-11-16 | Zeptometrix Corporation | Microorganismos que se han hecho no patogenos. |
| US6664056B2 (en) | 2000-10-17 | 2003-12-16 | The Chinese University Of Hong Kong | Non-invasive prenatal monitoring |
| US6551267B1 (en) | 2000-10-18 | 2003-04-22 | Becton, Dickinson And Company | Medical article having blood-contacting surface |
| US6602718B1 (en) | 2000-11-08 | 2003-08-05 | Becton, Dickinson And Company | Method and device for collecting and stabilizing a biological sample |
| JP5192631B2 (ja) | 2000-11-08 | 2013-05-08 | ベクトン・ディキンソン・アンド・カンパニー | 生物学的試料を収集し安定化させるための方法および装置 |
| EP1368369A4 (en) | 2000-11-15 | 2006-02-22 | Hoffmann La Roche | METHODS AND REAGENTS FOR IDENTIFYING RARE EMBRYONIC CELLS IN THE MATERNAL CIRCULATORY SYSTEM |
| EP1207208A3 (en) | 2000-11-15 | 2003-12-10 | Becton Dickinson and Company | Method for preservation of cells and nucleic acid targets |
| US20020066216A1 (en) * | 2000-12-01 | 2002-06-06 | Delacruz Cedric G. | Baby birth announcement |
| US6794152B2 (en) | 2000-12-22 | 2004-09-21 | Streck Laboratories Inc. | Flow cytometry reagent and system |
| US6581973B2 (en) * | 2001-03-30 | 2003-06-24 | Cheringal Associates, Inc. | Double blind study label |
| US6617180B1 (en) * | 2001-04-16 | 2003-09-09 | Taiwan Semiconductor Manufacturing Company | Test structure for detecting bridging of DRAM capacitors |
| FR2824144B1 (fr) * | 2001-04-30 | 2004-09-17 | Metagenex S A R L | Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel |
| ATE502119T1 (de) * | 2001-07-25 | 2011-04-15 | Oncomedx Inc | Verfahren zur bewertung pathologischer krankheitszustände unter verwendung extrazellulärer rna |
| WO2008111981A1 (en) | 2007-03-14 | 2008-09-18 | Sierra Molecular Corporation | Compositions, systems, and methods for preservation of macromolecules |
| US7863012B2 (en) | 2004-02-17 | 2011-01-04 | Veridex, Llc | Analysis of circulating tumor cells, fragments, and debris |
| WO2003019141A2 (en) | 2001-08-23 | 2003-03-06 | Immunivest Corporation | Analysis of circulating tumor cells, fragments, and debris |
| AU2002329951A1 (en) * | 2001-08-31 | 2003-03-18 | University Of North Carolina At Chapel Hill | Fixed-dried red blood cells |
| US6527242B1 (en) * | 2001-10-01 | 2003-03-04 | Netta Kennedy | Brace for a picture frame |
| WO2003063930A1 (en) * | 2002-02-01 | 2003-08-07 | Gambro, Inc. | Whole blood collection and processing method |
| GB0203280D0 (en) * | 2002-02-12 | 2002-03-27 | Ic Innovations Ltd | Anti-glycolytic composition |
| AU2003206946A1 (en) | 2002-02-22 | 2003-09-09 | Klaus Strebhardt | Agent for inhibiting development or progress of proliferative diseases and especially cancer diseases and pharmaceutical composition containing said agent |
| GB2386038A (en) | 2002-02-27 | 2003-09-03 | Motorola Inc | Channel estimation in a radio receiver |
| US6977162B2 (en) * | 2002-03-01 | 2005-12-20 | Ravgen, Inc. | Rapid analysis of variations in a genome |
| CN100389880C (zh) | 2002-05-07 | 2008-05-28 | 贝克顿·迪金森公司 | 采集组件 |
| US7442506B2 (en) | 2002-05-08 | 2008-10-28 | Ravgen, Inc. | Methods for detection of genetic disorders |
| US20070178478A1 (en) * | 2002-05-08 | 2007-08-02 | Dhallan Ravinder S | Methods for detection of genetic disorders |
| US7022478B2 (en) * | 2002-05-14 | 2006-04-04 | The Chinese University Of Hong Kong | Methods for evaluating stroke or cardiac ischemia by nucleic acid detection |
| US20040014107A1 (en) | 2002-05-22 | 2004-01-22 | Garcia-Blanco Mariano A. | RNP immunoprecipitation assay |
| WO2003106623A2 (en) | 2002-06-13 | 2003-12-24 | New York University | Early noninvasive prenatal test for aneuploidies and heritable conditions |
| US6913932B2 (en) * | 2002-08-23 | 2005-07-05 | Beckman Coulter, Inc. | Formaldehyde-ammonium salt complexes for the stabilization of blood cells |
| US20060008807A1 (en) * | 2002-08-23 | 2006-01-12 | O'hara Shawn M | Multiparameter analysis of comprehensive nucleic acids and morphological features on the same sample |
| EP1816461B1 (en) | 2002-10-16 | 2020-01-08 | Streck Laboratories, Inc. | Method and device for collecting and preserving cells for analysis |
| ES2784011T3 (es) | 2002-10-16 | 2020-09-21 | Streck Inc | Procedimiento y dispositivo para recoger y preservar las células para su análisis |
| KR100471183B1 (ko) * | 2002-10-29 | 2005-03-10 | 삼성전자주식회사 | 오프 세트 트랜지스터를 갖는 반도체 기억소자 및 그제조방법 |
| EP1599608A4 (en) * | 2003-03-05 | 2007-07-18 | Genetic Technologies Ltd | Identification of fetal DNA and fetal cell marker in maternal plasma or serum |
| US7267980B1 (en) | 2003-04-04 | 2007-09-11 | Research & Diagnostic Systems, Inc. | Stabilizing solution for cells and tissues |
| US20050277204A1 (en) | 2003-08-12 | 2005-12-15 | Massachusetts Institute Of Technology | Sample preparation methods and devices |
| US7318293B2 (en) * | 2003-09-19 | 2008-01-15 | Ardern Ii William B | Binder clip sleeve |
| US20070111233A1 (en) * | 2003-10-30 | 2007-05-17 | Bianchi Diana W | Prenatal diagnosis using cell-free fetal DNA in amniotic fluid |
| US20050181353A1 (en) * | 2004-02-17 | 2005-08-18 | Rao Galla C. | Stabilization of cells and biological specimens for analysis |
| US20100216153A1 (en) | 2004-02-27 | 2010-08-26 | Helicos Biosciences Corporation | Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities |
| WO2005108411A2 (en) * | 2004-05-05 | 2005-11-17 | Isis Pharmaceuticals, Inc. | Substituted pixyl protecting groups for oligonucleotide synthesis |
| US8497134B2 (en) | 2004-08-19 | 2013-07-30 | Blood Cell Storage, Inc. | Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices |
| ES2251307B2 (es) * | 2004-10-05 | 2006-12-16 | Universidad Complutense De Madrid | Solucion para el mantenimiento indefinido de los acidos nucleicos en su celula de origen. |
| KR20070097430A (ko) * | 2004-11-05 | 2007-10-04 | 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. | 안정화 시약으로부터 핵산을 정제하기 위한 조성물 및 방법 |
| WO2006061216A2 (en) | 2004-12-10 | 2006-06-15 | Bayer Healthcare Ag | Genetic alteration useful for the response prediction of malignant neoplasia to taxane-based medical treatment |
| US7390663B2 (en) | 2005-02-23 | 2008-06-24 | Streck, Inc. | Process, composition and kit for providing a stable whole blood calibrator/control |
| US7419832B2 (en) * | 2005-03-10 | 2008-09-02 | Streck, Inc. | Blood collection tube with surfactant |
| DE102005013261A1 (de) | 2005-03-22 | 2006-09-28 | Adnagen Ag | Reagenz und Verfahren zur Verhinderung der zeitabhängigen Expression in biologischen Zellen |
| US7545909B2 (en) | 2005-05-20 | 2009-06-09 | Imaging Sciences International Llc | Generating panoramic views of the jaw using a fixed reference point and crossing points of multiple rays |
| WO2007022483A2 (en) | 2005-08-19 | 2007-02-22 | Bioventures, Inc. | Method and device for the collection and isolation of nucleic acid |
| WO2007053491A2 (en) | 2005-10-28 | 2007-05-10 | The Trustees Of Columbia University In The City Of New York | Method and kit for evaluating rna quality |
| SI2385143T1 (sl) * | 2006-02-02 | 2016-11-30 | The Board of Trustees of the Leland Stanford Junior University Office of the General Counsel | Neinvazivni genetski presejalni test ploda z digitalno analizo |
| ATE508209T1 (de) * | 2006-02-28 | 2011-05-15 | Univ Louisville Res Found | Erkennung von chromosomabnormalitäten im fötus mit hilfe der tandem-einzelnukleotid- polymorphismen |
| US20070243548A1 (en) | 2006-03-28 | 2007-10-18 | Elias Georges | Calumenin-directed diagnostics and therapeutics for cancer and chemotherapeutic drug resistance |
| WO2007121276A2 (en) | 2006-04-12 | 2007-10-25 | Biocept, Inc. | Enrichment of circulating fetal dna |
| ES2264403B1 (es) | 2006-06-22 | 2007-11-01 | Grifols S.A. | Medio de suspension de hematies. |
| US20080081689A1 (en) * | 2006-07-07 | 2008-04-03 | Atlantic City Coin & Slot Service Company, Inc. | Gaming device and method of use |
| EP2057181B1 (en) | 2006-08-30 | 2012-02-08 | Bioventures, Inc. | Methods and substances for isolation and detection of small polynucleotides |
| US8080645B2 (en) | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| US20080108071A1 (en) * | 2006-10-10 | 2008-05-08 | Katherine Thompson | Methods and Systems to Determine Fetal Sex and Detect Fetal Abnormalities |
| GB0704488D0 (en) * | 2007-03-08 | 2007-04-18 | Univ Nottingham | Platelet viability kit |
| US8537789B2 (en) | 2007-08-01 | 2013-09-17 | Harris Corporation | Mobile ad-hoc network providing communication latency reduction features and related methods |
| US20090081678A1 (en) | 2007-09-21 | 2009-03-26 | Streck, Inc. | Nucleic acid isolation in preserved whole blood |
| US20090308303A1 (en) | 2008-06-13 | 2009-12-17 | Burlando Albert A | Identification marker |
| EP2297647A4 (en) | 2008-07-10 | 2012-12-12 | Rocketick Technologies Ltd | EFFICIENT PARALLEL CALCULATION OF DEPENDENCE PROBLEMS |
| US20100167271A1 (en) * | 2008-12-30 | 2010-07-01 | Streck, Inc. | Method for screening blood using a preservative that may be in a substantially solid state form |
| US11634747B2 (en) * | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| DK3290530T3 (da) | 2009-02-18 | 2020-12-07 | Streck Inc | Konservering af cellefrie nukleinsyrer |
| CN110470835A (zh) | 2009-03-24 | 2019-11-19 | 生物概念股份有限公司 | 细胞捕获和分析的装置和方法 |
| US20110027771A1 (en) * | 2009-07-31 | 2011-02-03 | Artemis Health, Inc. | Methods and compositions for cell stabilization |
| US20110053208A1 (en) * | 2009-08-31 | 2011-03-03 | Streck, Inc. | Biological sample identification system |
| EP3103883A1 (en) * | 2009-11-09 | 2016-12-14 | Streck, Inc. | Stabilization of rna in and extracting from intact cells within a blood sample |
| BR112012016451A2 (pt) | 2010-01-04 | 2020-09-08 | Giagen Gaithersburg, INC. | métodos, composições, e kits para recuperação de ácidos nucleicos ou proteínas de amostras de tecido fixadas |
| US9040255B2 (en) | 2010-09-23 | 2015-05-26 | Biocept, Inc. | Use of diazolidinyl urea for anti-clumping of biological samples |
| US20130316218A1 (en) | 2010-10-07 | 2013-11-28 | Schott Ag | Electrochemical energy accumulator |
| EP2642145A4 (en) | 2010-11-16 | 2015-10-21 | Ntn Toyo Bearing Co Ltd | BALL BEARING, AND METHOD FOR PRODUCING BALL BEARING |
| CA2830389A1 (en) | 2011-04-21 | 2012-10-26 | Streck, Inc. | Improved sample tube having particular utility for nucleic acid amplification |
| US9737891B2 (en) | 2011-06-01 | 2017-08-22 | Streck, Inc. | Rapid thermocycler system for rapid amplification of nucleic acids and related methods |
| MX2014006884A (es) | 2011-12-09 | 2015-06-02 | Gerd Marienfeld | Aparato, sistema y metodo para identificar celulas tumorales circulantes. |
| WO2013123030A2 (en) | 2012-02-13 | 2013-08-22 | Streck, Inc. | Blood collection device for improved nucleic acid regulation |
| EP2888363B1 (en) | 2012-08-21 | 2018-06-06 | Qiagen GmbH | Method for isolating nucleic acids from a formaldehyde releaser stabilized sample |
| US20140199681A1 (en) | 2013-01-14 | 2014-07-17 | Streck, Inc. | Blood collection device for stabilizing cell-free rna in blood during sample shipping and storage |
| CA2917912C (en) | 2013-07-24 | 2019-09-17 | Streck, Inc. | Compositions and methods for stabilizing circulating tumor cells |
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| EP2398912B1 (en) | 2017-09-13 |
| EP3778919A1 (en) | 2021-02-17 |
| US20190233879A1 (en) | 2019-08-01 |
| US9926590B2 (en) | 2018-03-27 |
| EP3290530A1 (en) | 2018-03-07 |
| US20240218425A1 (en) | 2024-07-04 |
| US11761025B2 (en) | 2023-09-19 |
| ES2649572T3 (es) | 2018-01-12 |
| NO2398912T3 (enExample) | 2018-02-10 |
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