CN112760360B - 一种rna保护剂及其应用 - Google Patents
一种rna保护剂及其应用 Download PDFInfo
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- CN112760360B CN112760360B CN202110302602.2A CN202110302602A CN112760360B CN 112760360 B CN112760360 B CN 112760360B CN 202110302602 A CN202110302602 A CN 202110302602A CN 112760360 B CN112760360 B CN 112760360B
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- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229960000789 guanidine hydrochloride Drugs 0.000 claims abstract description 14
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims abstract description 14
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- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
温度/时间 | 对比例1 | 对比例2 | 对比例3 | 对比例4 |
37℃/3日 | 15.38% | 10.51% | 11.13% | 7.56% |
37℃/5日 | 20.02% | 21.35% | 20.38% | 18.96% |
25℃/3日 | 6.93% | 5.68% | 5.23% | 3.09% |
25℃/7日 | 9.21% | 4.23% | 4.68% | 3.85% |
4℃/2周 | 10.01% | 5.98% | 5.21% | 2.63% |
4℃/4周 | 13.49% | 10.59% | 9.38% | 5.26% |
-20℃/长期 | 0.67% | 0.66% | 0.60% | 0.65% |
-80℃/长期 | 0.59% | 0.55% | 0.51% | 0.61% |
Claims (8)
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CN202110302602.2A CN112760360B (zh) | 2021-03-22 | 2021-03-22 | 一种rna保护剂及其应用 |
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CN202110302602.2A CN112760360B (zh) | 2021-03-22 | 2021-03-22 | 一种rna保护剂及其应用 |
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CN112760360A CN112760360A (zh) | 2021-05-07 |
CN112760360B true CN112760360B (zh) | 2023-12-19 |
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Families Citing this family (1)
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CN117165580B (zh) * | 2023-11-03 | 2024-01-26 | 青岛金域医学检验实验室有限公司 | 一种用于稳定样本中核酸的组合物、制备方法及其应用 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103881978A (zh) * | 2014-03-05 | 2014-06-25 | 成都诺恩生物科技有限公司 | 一种rna保存液、包含该保存液的试剂盒及其应用 |
CN105392363A (zh) * | 2013-03-01 | 2016-03-09 | A·S·戈尔兹伯勒 | 样品的固定和稳定化 |
CN106065400A (zh) * | 2015-04-24 | 2016-11-02 | 上海润腾生物科技有限公司 | 一种核糖核酸保护剂、试剂盒、应用及保存方法 |
CN108473932A (zh) * | 2015-09-09 | 2018-08-31 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
CN109486904A (zh) * | 2018-12-29 | 2019-03-19 | 广州阳普医疗科技股份有限公司 | 一种全血rna保存液及其应用 |
CN109609498A (zh) * | 2019-01-28 | 2019-04-12 | 温州医科大学附属第医院 | 一种动物组织rna的样品保存液及其保存方法 |
CN110760567A (zh) * | 2019-11-12 | 2020-02-07 | 杭州昱鼎生物科技有限公司 | 尿液样本rna稳定液及制备方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9683256B2 (en) * | 2007-10-01 | 2017-06-20 | Longhorn Vaccines And Diagnostics, Llc | Biological specimen collection and transport system |
US8304187B2 (en) * | 2009-02-18 | 2012-11-06 | Streck, Inc. | Preservation of cell-free RNA in blood samples |
WO2017214338A1 (en) * | 2016-06-07 | 2017-12-14 | Drawbridge Health, Inc. | Metods and devices for strong or stabilizing molecules |
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2021
- 2021-03-22 CN CN202110302602.2A patent/CN112760360B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105392363A (zh) * | 2013-03-01 | 2016-03-09 | A·S·戈尔兹伯勒 | 样品的固定和稳定化 |
CN103881978A (zh) * | 2014-03-05 | 2014-06-25 | 成都诺恩生物科技有限公司 | 一种rna保存液、包含该保存液的试剂盒及其应用 |
CN106065400A (zh) * | 2015-04-24 | 2016-11-02 | 上海润腾生物科技有限公司 | 一种核糖核酸保护剂、试剂盒、应用及保存方法 |
CN108473932A (zh) * | 2015-09-09 | 2018-08-31 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
CN109486904A (zh) * | 2018-12-29 | 2019-03-19 | 广州阳普医疗科技股份有限公司 | 一种全血rna保存液及其应用 |
CN109609498A (zh) * | 2019-01-28 | 2019-04-12 | 温州医科大学附属第医院 | 一种动物组织rna的样品保存液及其保存方法 |
CN110760567A (zh) * | 2019-11-12 | 2020-02-07 | 杭州昱鼎生物科技有限公司 | 尿液样本rna稳定液及制备方法 |
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