DK176090B1 - Fremgangsmåde til oprensning af proteiner - Google Patents

Fremgangsmåde til oprensning af proteiner Download PDF

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Publication number
DK176090B1
DK176090B1 DK198904855A DK485589A DK176090B1 DK 176090 B1 DK176090 B1 DK 176090B1 DK 198904855 A DK198904855 A DK 198904855A DK 485589 A DK485589 A DK 485589A DK 176090 B1 DK176090 B1 DK 176090B1
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DK
Denmark
Prior art keywords
protein
resin
ion exchange
cation
vitamin
Prior art date
Application number
DK198904855A
Other languages
Danish (da)
English (en)
Other versions
DK485589A (da
DK485589D0 (da
Inventor
Sau-Chi Betty Yan
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26943089&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK176090(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of DK485589D0 publication Critical patent/DK485589D0/da
Publication of DK485589A publication Critical patent/DK485589A/da
Application granted granted Critical
Publication of DK176090B1 publication Critical patent/DK176090B1/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
DK198904855A 1988-10-04 1989-10-03 Fremgangsmåde til oprensning af proteiner DK176090B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US25327988A 1988-10-04 1988-10-04
US25327988 1988-10-04
US39328189 1989-08-16
US07/393,281 US4981952A (en) 1988-10-04 1989-08-16 Method for the purification of vitamin K-dependent proteins

Publications (3)

Publication Number Publication Date
DK485589D0 DK485589D0 (da) 1989-10-03
DK485589A DK485589A (da) 1990-04-05
DK176090B1 true DK176090B1 (da) 2006-05-22

Family

ID=26943089

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198904855A DK176090B1 (da) 1988-10-04 1989-10-03 Fremgangsmåde til oprensning af proteiner

Country Status (13)

Country Link
US (1) US4981952A (ja)
EP (1) EP0363126B2 (ja)
JP (1) JP2848461B2 (ja)
KR (1) KR0139209B1 (ja)
AT (1) ATE106406T1 (ja)
AU (1) AU635222B2 (ja)
CA (1) CA1314011C (ja)
DE (1) DE68915675T3 (ja)
DK (1) DK176090B1 (ja)
ES (1) ES2054019T5 (ja)
HU (1) HU204538B (ja)
IE (1) IE63765B1 (ja)
IL (1) IL91822A (ja)

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* Cited by examiner, † Cited by third party
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GB8729822D0 (en) * 1987-12-22 1988-02-03 Central Blood Lab Authority Chemical process
DE3833936C1 (ja) * 1988-10-05 1989-09-21 Henning Berlin Gmbh Chemie- Und Pharmawerk, 1000 Berlin, De
DE3911629A1 (de) * 1989-04-10 1990-10-11 Behringwerke Ag Verfahren zur abtrennung von toxinen aus proteinloesungen
IL97312A (en) 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
DK0573605T3 (da) * 1991-03-01 2001-01-02 Rhone Poulenc Rorer Int Fremstilling af faktor IX
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
MY110664A (en) * 1992-05-21 1999-01-30 Lilly Co Eli Protein c derivatives
DK38293D0 (da) * 1993-03-31 1993-03-31 Novo Nordisk As Fremstilling af proteiner
US5618714A (en) * 1993-12-15 1997-04-08 Eli Lilly And Company Methods for producing protein C
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
DE4435520A1 (de) * 1994-10-04 1996-04-11 Immuno Ag Verfahren zur Trennung von rekombinantem pro-Faktor IX von rekombinantem Faktor IX
US5714583A (en) 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
US5910584A (en) * 1996-09-05 1999-06-08 Asahi Kogaku Kogyo Kabushiki Kaisha Method for isolating plasmid DNA
US5843731A (en) * 1996-09-05 1998-12-01 Asahi Kogaku Kogyo Kabushiki Kaisha Method for purifying plasmid DNA on calcium phosphate compound
US6630137B1 (en) 1997-04-28 2003-10-07 Eli Lilly And Company Activated protein C formulations
WO1998048818A1 (en) * 1997-04-28 1998-11-05 Eli Lilly And Company Activated protein c formulations
EP1557463A1 (en) * 1997-04-28 2005-07-27 Eli Lilly & Company Improved methods for processing activated protein C
CA2293429A1 (en) * 1997-06-05 1998-12-10 Eli Lilly And Company Methods for treating thrombotic disorders
HUP0001237A3 (en) * 1997-10-20 2002-01-28 Lilly Co Eli Methods for treating vascular disorders
CA2347250A1 (en) 1998-10-22 2000-04-27 Sau-Chi Betty Yan Methods for treating sepsis
AU1477000A (en) 1998-11-13 2000-06-05 Eli Lilly And Company Method of treating heparin-induced thrombocytopenia
BR9915460A (pt) 1998-11-20 2001-07-17 Lilly Co Eli Método de tratamento de febre hemorrágica virótica
ATE232739T1 (de) 1998-11-23 2003-03-15 Lilly Co Eli Protein c zur behandlung von sichelzellanämie und thalassämie
US7204981B2 (en) * 2000-03-28 2007-04-17 Eli Lilly And Company Methods of treating diseases with activated protein C
WO2001089558A2 (en) 2000-05-24 2001-11-29 Eli Lilly And Company Formulations and use of activated protein c and protein c zymogen for treating hypercoagulable states
CA2385371A1 (en) * 2000-07-21 2002-01-31 Hiroshi Mizokami Method of purifying calcium ion-binding protein
WO2002085117A1 (en) * 2001-04-24 2002-10-31 Eisai Co., Ltd. Methods and compositions for preventing and treating septic shock and endotoxemia
JP2004535461A (ja) * 2001-07-19 2004-11-25 ディーエムアイ バイオサイエンシズ インコーポレイテッド プロテインｃの不活性化を抑制するための銅キレート剤の使用
JP4628618B2 (ja) * 2001-09-26 2011-02-09 富士フイルム株式会社 撮像光学系
EP1485121A4 (en) * 2002-03-08 2007-11-07 Lilly Co Eli ACTIVE C PROTEIN FORMULATIONS
WO2004056309A2 (en) 2002-12-05 2004-07-08 Socratech L.L.C. Neuroprotective activity of activated protein c is independent of its anticoagulant activity
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
KR101284884B1 (ko) * 2003-07-08 2013-07-10 더 스크립스 리서치 인스티튜트 정상의 세포보호 활성 및 저하된 항응고 활성을 지닌활성화 단백질 ｃ 변이체
US9192657B2 (en) * 2003-07-08 2015-11-24 The Scripps Research Institute Activated protein C variants with normal cytoprotective activity but reduced anticoagulant activity
US20080305100A1 (en) * 2004-07-23 2008-12-11 Zlokovic Berislav V Activated Protein C Inhibits Undesirable Effects of Plasminogen Activator in the Brain
PL1831242T3 (pl) 2004-12-23 2013-04-30 Novo Nordisk Healthcare Ag Zmniejszanie zawartości zanieczyszczeń białkowych w kompozycjach zawierających zależne od witaminy K białko będące przedmiotem zainteresowania
ATE476501T1 (de) * 2005-01-14 2010-08-15 Bayer Healthcare Llc Verfahren zur reinigung von factor vii
US8088728B2 (en) * 2005-06-24 2012-01-03 Drugrecure Aps Airway administration of tissue factor pathway inhibitor in inflammatory conditions affecting the respiratory tract
WO2007071767A1 (en) * 2005-12-23 2007-06-28 Novo Nordisk Health Care Ag Purification of vitamin k-dependent polypeptides using preparative reverse phase chromatography (rpc)
DK2004683T3 (en) * 2006-03-24 2016-08-22 Biogen Hemophilia Inc PC5 AS A FACTOR IX PROPEPTID PROCESSING ENZYM
WO2008073603A2 (en) * 2006-10-31 2008-06-19 The Scripps Research Institute Dosing regimen of activated protein c and variants having reduced anticoagulant activity
WO2008104372A1 (en) * 2007-02-28 2008-09-04 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules
CA2683423C (en) 2007-04-26 2020-10-27 Inspiration Biopharmaceuticals, Inc. Recombinant vitamin k dependent proteins with high sialic acid content and methods of preparing same
LT3597659T (lt) 2007-07-09 2023-05-10 Genentech, Inc. Disulfidinės jungties redukcijos prevencijos būdas gaminant polipeptidą rekombinantiniu būdu
ES2576109T3 (es) * 2008-12-02 2016-07-05 Novo Nordisk Health Care Ag Purificación de polipéptidos
EP2358874B1 (en) 2008-12-19 2017-03-29 INSERM (Institut National de la Santé et de la Recherche Médicale) Serine protease derivatives and uses in the prevention or the treatment of blood coagulation disorders
EP2199387A1 (en) 2008-12-19 2010-06-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Serine protease derivatives and uses for the prevention and/or the treatment of blood coagulation disorders
JP5851410B2 (ja) 2009-10-30 2016-02-03 シーエヌジェイ ホールディングス、インク． 組換えビタミンｋ依存性タンパク質の生成法
BR112012017696A2 (pt) 2010-01-18 2017-10-03 Novo Nordisk Healthcare Ag Purificação de fatores de coagulação de sangue
CN106967150B (zh) 2010-04-29 2020-12-04 百深公司 二价阳离子结合蛋白在阴离子交换树脂上的纯化方法
CN107915769A (zh) 2011-03-29 2018-04-17 葛兰素史密斯克莱有限责任公司 用于蛋白纯化的缓冲液体系
AU2012304763A1 (en) 2011-09-06 2014-03-06 Medimmune Llc Methods for processing coagulation factors
US10378004B2 (en) 2011-10-14 2019-08-13 Baxalta GmbH Protein purification by anion exchange chromatography
EP2748180B1 (en) 2011-10-14 2018-08-15 Baxalta GmbH Protein purification by anion exchange chromatography
KR102068010B1 (ko) 2012-07-04 2020-01-20 제트제트 바이오테크 엘엘씨 염증성 피부 질환의 치료
PL2970376T3 (pl) * 2013-03-15 2018-10-31 Baxalta Incorporated Sposób oczyszczania białek zależnych od witaminy k za pomocą chromatografii anionowymiennej
CN106573040B (zh) 2014-04-16 2021-07-06 Zz生物技术有限责任公司 治疗异常皮肤瘢痕形成
US20180369346A1 (en) * 2016-01-07 2018-12-27 Eio Biomedical Ltd. Methods, compositions and kits for reducing tissue adhesions

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DE3101752A1 (de) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "verfahren zur reinigung der blutgerinnungsfaktoren ii, vii, ix und/oder x und danach hergestellte praeparationen"
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Also Published As

Publication number Publication date
KR0139209B1 (ko) 1998-04-30
ES2054019T3 (es) 1994-08-01
AU635222B2 (en) 1993-03-18
JP2848461B2 (ja) 1999-01-20
EP0363126A3 (en) 1991-09-11
EP0363126B2 (en) 2002-03-06
ATE106406T1 (de) 1994-06-15
IL91822A (en) 2001-06-14
EP0363126A2 (en) 1990-04-11
JPH02200180A (ja) 1990-08-08
DK485589A (da) 1990-04-05
IE893159L (en) 1990-04-04
EP0363126B1 (en) 1994-06-01
IL91822A0 (en) 1990-06-10
AU4251989A (en) 1990-04-12
IE63765B1 (en) 1995-06-14
DK485589D0 (da) 1989-10-03
DE68915675T2 (de) 1994-10-20
KR900006511A (ko) 1990-05-08
US4981952A (en) 1991-01-01
ES2054019T5 (es) 2002-10-16
DE68915675D1 (de) 1994-07-07
CA1314011C (en) 1993-03-02
DE68915675T3 (de) 2002-08-14
HU204538B (en) 1992-01-28
HUT53373A (en) 1990-10-28

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