DK175036B1 - Fremgangsmåde til fremstilling af et polypeptid ved dyrkning af en transformeret mikroorganisme, en transformeret mikroorganisme egnet dertil og DNA-sekvenser, som er egnede til fremstilling af en sådan mikroorganisme - Google Patents

Fremgangsmåde til fremstilling af et polypeptid ved dyrkning af en transformeret mikroorganisme, en transformeret mikroorganisme egnet dertil og DNA-sekvenser, som er egnede til fremstilling af en sådan mikroorganisme Download PDF

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DK175036B1
DK175036B1 DK342785A DK342785A DK175036B1 DK 175036 B1 DK175036 B1 DK 175036B1 DK 342785 A DK342785 A DK 342785A DK 342785 A DK342785 A DK 342785A DK 175036 B1 DK175036 B1 DK 175036B1
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leu
gly
asp
val
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DK342785A
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DK342785D0 (da
DK342785A (da
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Cornelis Theodorus Verrips
Adrianus Marinus Ledeboer
Jan Maat
Christiaan Visser
Zbigniew Alojzy Janowicz
Cornelis Petrus Hollenberg
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Unilever Nv
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DK342785A 1984-07-27 1985-07-26 Fremgangsmåde til fremstilling af et polypeptid ved dyrkning af en transformeret mikroorganisme, en transformeret mikroorganisme egnet dertil og DNA-sekvenser, som er egnede til fremstilling af en sådan mikroorganisme DK175036B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP84201114 1984-07-27
EP84201114 1984-07-27
GB8503160 1985-02-07
GB858503160A GB8503160D0 (en) 1985-02-07 1985-02-07 Enzymes

Publications (3)

Publication Number Publication Date
DK342785D0 DK342785D0 (da) 1985-07-26
DK342785A DK342785A (da) 1986-01-28
DK175036B1 true DK175036B1 (da) 2004-05-10

Family

ID=26092832

Family Applications (1)

Application Number Title Priority Date Filing Date
DK342785A DK175036B1 (da) 1984-07-27 1985-07-26 Fremgangsmåde til fremstilling af et polypeptid ved dyrkning af en transformeret mikroorganisme, en transformeret mikroorganisme egnet dertil og DNA-sekvenser, som er egnede til fremstilling af en sådan mikroorganisme

Country Status (5)

Country Link
EP (2) EP0423890A3 (ja)
JP (4) JP2592444B2 (ja)
CA (1) CA1341130C (ja)
DE (1) DE3583194D1 (ja)
DK (1) DK175036B1 (ja)

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US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
DE3650783T2 (de) * 1985-04-15 2004-07-15 Dsm Ip Assets B.V. Verwendung des Glucoamylasepromotors aus Apergillus
US5198345A (en) * 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
EP0245497A4 (en) * 1985-11-12 1989-06-27 American Biogenetics Corp BIOGENETIC CASSETTE.
CA1339101C (en) * 1986-06-03 1997-07-29 Nicolaas Overbeeke Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
NL8601454A (nl) * 1986-06-05 1988-01-04 Unilever Nv Werkwijze voor het bereiden van een catalase-vrij oxidoreductase en van een catalase-vrije oxidoreductase bevattende gist, en gebruik daarvan.
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
DE3765978D1 (de) * 1986-11-24 1990-12-13 Unilever Nv Verfahren zur herstellung einer catalasefreien, oxidase enthaltenden hefe und deren verwendung.
DK212388D0 (da) * 1988-04-15 1988-04-15 Novo Industri As Detergent additiv
GB8826401D0 (en) * 1988-11-11 1988-12-14 Unilever Plc Bleach composition
ATE111524T1 (de) * 1988-12-20 1994-09-15 Rhein Biotech Ges Fuer Biotech Mutanter stamm von einem methylotrophen organismus und verfahren zur herstellung eines proteins in methylotrophen organismus.
JP3273609B2 (ja) * 1989-07-07 2002-04-08 ユニリーバー・ナームローゼ・ベンノートシヤープ 発現ベクターのマルチコピー組込みにより形質転換した真菌による蛋白質の製法
FR2656530B1 (fr) * 1989-12-29 1994-09-23 Sanofi Sa Gene recombinant pour une expression dans les cellules eucaryotes d'une proteine telle que l'urate oxydase.
NZ234453A (en) * 1989-07-13 1993-01-27 Sanofi Sa Recombinant dna encoding urate oxidase, and vector, host, protein and pharmaceutical compositions associated therewith
FR2649720A1 (en) * 1989-07-13 1991-01-18 Sanofi Sa Recombinant gene which encodes a protein such as urate oxidase
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
US5273896A (en) * 1989-10-13 1993-12-28 Novo Nordisk A/S Hemopeptide having peroxidase activity for bleaching dyes
PE14291A1 (es) * 1989-10-13 1991-04-27 Novo Nordisk As Procedimiento para inhibir la transferencia de tintes
JPH06500470A (ja) * 1990-09-04 1994-01-20 メルク　エンド　カンパニー　インコーポレーテッド メチロトローフ酵母細胞におけるインスリン様成長因子の産生
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5919684A (en) * 1991-03-11 1999-07-06 Moldowan Lab Inc. Process for the reduction of catalase activity in alcohol oxidase
WO1992016634A1 (en) * 1991-03-22 1992-10-01 Novo Nordisk A/S A process for producing heme proteins
ES2177528T3 (es) * 1991-04-01 2002-12-16 Merck & Co Inc Genes que influyen en la actividad proteolitica de pichia y usoso de los mismos.
US5204261A (en) * 1991-04-24 1993-04-20 Phillips Petroleum Company Catalase-negative pichia pastoris
EP0632128A4 (en) * 1992-03-02 1996-04-24 Kyowa Hakko Kogyo Kk NEW PLANT GENE.
EP0690921B1 (en) * 1993-03-03 1997-05-28 E.I. Du Pont De Nemours And Company Production of glycolate oxidase in methylotrophic yeast
DE4329969A1 (de) * 1993-09-04 1995-03-09 Basf Ag Verfahren zur rekombinanten Herstellung von Proteinen in Hefe
DE4430327C1 (de) * 1994-08-27 1996-05-09 Degussa Enzymatische, aktiven Sauerstoff liefernde Mischung sowie Verfahren zur enzymatischen Herstellung von Persäuren
US5747661A (en) * 1995-01-13 1998-05-05 Howard Hughes Medical Institute Retinoid-inducible response elements
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Also Published As

Publication number Publication date
EP0173378A3 (en) 1986-08-06
CA1341130C (en) 2000-10-31
EP0423890A2 (en) 1991-04-24
EP0423890A3 (en) 1991-07-03
JP2592444B2 (ja) 1997-03-19
JP2675202B2 (ja) 1997-11-12
DE3583194D1 (de) 1991-07-18
EP0173378B1 (en) 1991-06-12
JP2575284B2 (ja) 1997-01-22
JPH06292572A (ja) 1994-10-21
JPH09107969A (ja) 1997-04-28
DK342785D0 (da) 1985-07-26
JPS6192569A (ja) 1986-05-10
JPH04252181A (ja) 1992-09-08
EP0173378A2 (en) 1986-03-05
DK342785A (da) 1986-01-28

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