JPS565093A - Preparation of heat-resistant enzyme - Google Patents

Preparation of heat-resistant enzyme

Info

Publication number
JPS565093A
JPS565093A JP7876379A JP7876379A JPS565093A JP S565093 A JPS565093 A JP S565093A JP 7876379 A JP7876379 A JP 7876379A JP 7876379 A JP7876379 A JP 7876379A JP S565093 A JPS565093 A JP S565093A
Authority
JP
Japan
Prior art keywords
microorganism
dna
thermus
heat
resistant enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP7876379A
Other languages
Japanese (ja)
Other versions
JPS6158159B2 (en
Inventor
Kenji Sakaguchi
Kenji Nagahari
Takako Koshikawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP7876379A priority Critical patent/JPS565093A/en
Publication of JPS565093A publication Critical patent/JPS565093A/en
Publication of JPS6158159B2 publication Critical patent/JPS6158159B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:A hybrid DNA having genes of a microorganism which belongs to Thermus is introduced into the arrangement of the nucleotide to change the properties of a microorganism so that it grows at room temperature to produce selectively said heat-resistant enzyme. CONSTITUTION:A gene of a microorganism belonging to Thermus, e.g., chromosome DNA of Thermus theremophilus HB8 (ATCC-27634), and a plasmid DNA as a vector are digested with a restriction enzyme by a usual method and rebound with a ligase to prepare a hybride DNA having a gene of a microorganism belonging to Thermus in the nucleotide arrangement. The resultant recombined DNA is introduced into a microorganism which grows at room temperature such as Escherishia coli C600 (FERM-P5044) to produce a modified microorganism, which is cultured. The cells are collected, crushed, then the crushed mixture is heated to deactivate enzymes with no heat resistance, resulting in collection of said heat-resistant enzyme.
JP7876379A 1979-06-22 1979-06-22 Preparation of heat-resistant enzyme Granted JPS565093A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7876379A JPS565093A (en) 1979-06-22 1979-06-22 Preparation of heat-resistant enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7876379A JPS565093A (en) 1979-06-22 1979-06-22 Preparation of heat-resistant enzyme

Publications (2)

Publication Number Publication Date
JPS565093A true JPS565093A (en) 1981-01-20
JPS6158159B2 JPS6158159B2 (en) 1986-12-10

Family

ID=13670934

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7876379A Granted JPS565093A (en) 1979-06-22 1979-06-22 Preparation of heat-resistant enzyme

Country Status (1)

Country Link
JP (1) JPS565093A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60180590A (en) * 1984-02-24 1985-09-14 Unitika Ltd Plasmid
JPS60180580A (en) * 1984-02-24 1985-09-14 Unitika Ltd Escherichia coli
JPS6185060A (en) * 1984-09-29 1986-04-30 Toshiba Corp Noncontact positioning device
JPS6192569A (en) * 1984-07-27 1986-05-10 ユニリ−バ−　ナ−ムロ−ゼ　ベンノ−トシヤ−プ Preparation of oxydoreductase

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60180590A (en) * 1984-02-24 1985-09-14 Unitika Ltd Plasmid
JPS60180580A (en) * 1984-02-24 1985-09-14 Unitika Ltd Escherichia coli
JPH0532024B2 (en) * 1984-02-24 1993-05-14 Unitika Ltd
JPS6192569A (en) * 1984-07-27 1986-05-10 ユニリ−バ−　ナ−ムロ−ゼ　ベンノ−トシヤ−プ Preparation of oxydoreductase
JPH04252181A (en) * 1984-07-27 1992-09-08 Unilever Nv Method for production of oxidoreductase
JPH06292572A (en) * 1984-07-27 1994-10-21 Unilever Nv Oxidoreductase
JP2575284B2 (en) * 1984-07-27 1997-01-22 ユニリーバー・ナームローゼ・ベンノートシヤープ DNA encoding oxidoreductase
JPS6185060A (en) * 1984-09-29 1986-04-30 Toshiba Corp Noncontact positioning device
JPH0424946B2 (en) * 1984-09-29 1992-04-28 Tokyo Shibaura Electric Co

Also Published As

Publication number Publication date
JPS6158159B2 (en) 1986-12-10

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