DK0823479T3 - Modificeret termostabil DNA-polymerase - Google Patents

Modificeret termostabil DNA-polymerase

Info

Publication number
DK0823479T3
DK0823479T3 DK97113182T DK97113182T DK0823479T3 DK 0823479 T3 DK0823479 T3 DK 0823479T3 DK 97113182 T DK97113182 T DK 97113182T DK 97113182 T DK97113182 T DK 97113182T DK 0823479 T3 DK0823479 T3 DK 0823479T3
Authority
DK
Denmark
Prior art keywords
residue
sequence
dna
polymerases
thermostable dna
Prior art date
Application number
DK97113182T
Other languages
Danish (da)
English (en)
Inventor
David Harrow Gelfand
Lisa Vivian Kalman
Fred Lawrence Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0823479T3 publication Critical patent/DK0823479T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK97113182T 1996-08-06 1997-07-31 Modificeret termostabil DNA-polymerase DK0823479T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2337696P 1996-08-06 1996-08-06
EP97113182A EP0823479B1 (en) 1996-08-06 1997-07-31 Modified thermostable DNA polymerase

Publications (1)

Publication Number Publication Date
DK0823479T3 true DK0823479T3 (da) 2005-01-31

Family

ID=21814728

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97113182T DK0823479T3 (da) 1996-08-06 1997-07-31 Modificeret termostabil DNA-polymerase

Country Status (21)

Country Link
US (1) US5939292A (pt)
EP (1) EP0823479B1 (pt)
JP (1) JP3170229B2 (pt)
KR (1) KR100510619B1 (pt)
CN (1) CN1154733C (pt)
AT (1) ATE278020T1 (pt)
AU (1) AU714929B2 (pt)
BR (1) BR9704260B1 (pt)
CA (1) CA2210951C (pt)
CZ (1) CZ293215B6 (pt)
DE (1) DE69730926T2 (pt)
DK (1) DK0823479T3 (pt)
ES (1) ES2227640T3 (pt)
HU (1) HUP9701341A3 (pt)
IL (1) IL121441A (pt)
MX (1) MX9705961A (pt)
NO (1) NO322135B1 (pt)
PL (1) PL321478A1 (pt)
RU (1) RU2235773C2 (pt)
TW (1) TW528802B (pt)
UA (1) UA47423C2 (pt)

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US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
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US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
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US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
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US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US20070020622A1 (en) * 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
EP1493824A1 (en) * 2003-07-02 2005-01-05 Consortium National de Recherche en Genomique (CNRG) Method for detection of mutations in DNA
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
US20080311627A1 (en) * 2004-06-17 2008-12-18 Epigenomics Ag Compositions and Methods for Preventing Carry-Over Contamination in Nucleic Acid Amplification Reactions
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US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
US7645866B2 (en) * 2005-06-28 2010-01-12 Life Technologies Corporation Methods of producing and sequencing modified polynucleotides
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US20070154894A1 (en) * 2005-12-30 2007-07-05 Affymetrix, Inc. Labeling and non-enzymatic fragmentation of cDNA using a ribonucleoside triphosphate analog
US7993832B2 (en) 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8148067B2 (en) 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
JPWO2012070618A1 (ja) 2010-11-24 2014-05-19 株式会社カネカ 増幅核酸検出方法及び検出デバイス
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US9783844B2 (en) * 2012-04-27 2017-10-10 Kaneka Corporation Method for amplifying nucleic acid and method for detecting amplified nucleic acid
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ES2713653T3 (es) 2013-03-15 2019-05-23 Ibis Biosciences Inc Análogos nucleotídicos para secuenciación
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Also Published As

Publication number Publication date
IL121441A (en) 2000-07-16
DE69730926D1 (de) 2004-11-04
MX9705961A (es) 1998-02-28
IL121441A0 (en) 1998-01-04
KR19980018395A (ko) 1998-06-05
CZ229997A3 (cs) 1998-06-17
HUP9701341A2 (hu) 1999-06-28
HUP9701341A3 (en) 2000-12-28
EP0823479A2 (en) 1998-02-11
ATE278020T1 (de) 2004-10-15
ES2227640T3 (es) 2005-04-01
US5939292A (en) 1999-08-17
CA2210951C (en) 2009-02-10
EP0823479A3 (en) 2000-03-29
NO322135B1 (no) 2006-08-21
PL321478A1 (en) 1998-02-16
BR9704260A (pt) 1998-09-15
JPH1066588A (ja) 1998-03-10
CZ293215B6 (cs) 2004-03-17
NO973595L (no) 1998-02-09
NO973595D0 (no) 1997-08-05
BR9704260B1 (pt) 2010-09-08
DE69730926T2 (de) 2006-02-23
HU9701341D0 (en) 1997-09-29
AU3319797A (en) 1998-02-12
CN1177640A (zh) 1998-04-01
AU714929B2 (en) 2000-01-13
CN1154733C (zh) 2004-06-23
EP0823479B1 (en) 2004-09-29
CA2210951A1 (en) 1998-02-06
TW528802B (en) 2003-04-21
JP3170229B2 (ja) 2001-05-28
KR100510619B1 (ko) 2005-12-21
RU2235773C2 (ru) 2004-09-10
UA47423C2 (uk) 2002-07-15

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