DE69721282T2 - In vitro transpositionsystem unter verwendung einer modifizierten tn5 transposase - Google Patents

In vitro transpositionsystem unter verwendung einer modifizierten tn5 transposase

Info

Publication number
DE69721282T2
DE69721282T2 DE69721282T DE69721282T DE69721282T2 DE 69721282 T2 DE69721282 T2 DE 69721282T2 DE 69721282 T DE69721282 T DE 69721282T DE 69721282 T DE69721282 T DE 69721282T DE 69721282 T2 DE69721282 T2 DE 69721282T2
Authority
DE
Germany
Prior art keywords
transposase
modified
vitro transposition
transposition system
outside end
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69721282T
Other languages
English (en)
Other versions
DE69721282D1 (de
Inventor
S Reznikoff
Yu Goryshin
Hong Zhou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/814,877 external-priority patent/US5965443A/en
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Application granted granted Critical
Publication of DE69721282D1 publication Critical patent/DE69721282D1/de
Publication of DE69721282T2 publication Critical patent/DE69721282T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
DE69721282T 1996-09-09 1997-09-09 In vitro transpositionsystem unter verwendung einer modifizierten tn5 transposase Expired - Lifetime DE69721282T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/814,877 US5965443A (en) 1996-09-09 1996-09-09 System for in vitro transposition
US08/850,880 US5925545A (en) 1996-09-09 1997-05-02 System for in vitro transposition
PCT/US1997/015941 WO1998010077A1 (en) 1996-09-09 1997-09-09 System for in vitro transposition using modified tn5 transposase

Publications (2)

Publication Number Publication Date
DE69721282D1 DE69721282D1 (de) 2003-05-28
DE69721282T2 true DE69721282T2 (de) 2003-12-18

Family

ID=27123892

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69721282T Expired - Lifetime DE69721282T2 (de) 1996-09-09 1997-09-09 In vitro transpositionsystem unter verwendung einer modifizierten tn5 transposase

Country Status (12)

Country Link
US (3) US5925545A (de)
EP (1) EP0927258B1 (de)
JP (1) JP4361971B2 (de)
CN (1) CN1163605C (de)
AT (1) ATE238422T1 (de)
AU (1) AU732130B2 (de)
CA (1) CA2265477C (de)
DE (1) DE69721282T2 (de)
ES (1) ES2195171T3 (de)
PL (1) PL193220B1 (de)
RU (1) RU2218406C2 (de)
WO (1) WO1998010077A1 (de)

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Also Published As

Publication number Publication date
US5948622A (en) 1999-09-07
EP0927258A1 (de) 1999-07-07
US6437109B1 (en) 2002-08-20
ES2195171T3 (es) 2003-12-01
AU732130B2 (en) 2001-04-12
US5925545A (en) 1999-07-20
RU2218406C2 (ru) 2003-12-10
PL193220B1 (pl) 2007-01-31
JP4361971B2 (ja) 2009-11-11
ATE238422T1 (de) 2003-05-15
CA2265477C (en) 2007-03-20
CN1163605C (zh) 2004-08-25
CA2265477A1 (en) 1998-03-12
CN1251135A (zh) 2000-04-19
EP0927258B1 (de) 2003-04-23
AU4262097A (en) 1998-03-26
JP2001507565A (ja) 2001-06-12
WO1998010077A1 (en) 1998-03-12
PL332145A1 (en) 1999-08-30
DE69721282D1 (de) 2003-05-28

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