DE69611894T2 - Unsterbliche humane Hautzell-Linien und serumfreies Medium für ihre Herstellung - Google Patents

Unsterbliche humane Hautzell-Linien und serumfreies Medium für ihre Herstellung

Info

Publication number
DE69611894T2
DE69611894T2 DE69611894T DE69611894T DE69611894T2 DE 69611894 T2 DE69611894 T2 DE 69611894T2 DE 69611894 T DE69611894 T DE 69611894T DE 69611894 T DE69611894 T DE 69611894T DE 69611894 T2 DE69611894 T2 DE 69611894T2
Authority
DE
Germany
Prior art keywords
keratinocytes
medium
melanocytes
immortalized
serum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69611894T
Other languages
German (de)
English (en)
Other versions
DE69611894D1 (de
Inventor
Markus Baur
Catherine Mace
Armand Malnoe
Andrea M. A. Pfeifer
Marcelle Regnier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe des Produits Nestle SA
Nestle SA
Original Assignee
Societe des Produits Nestle SA
Nestle SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Societe des Produits Nestle SA, Nestle SA filed Critical Societe des Produits Nestle SA
Application granted granted Critical
Publication of DE69611894D1 publication Critical patent/DE69611894D1/de
Publication of DE69611894T2 publication Critical patent/DE69611894T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0625Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629Keratinocytes; Whole skin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • CCHEMISTRY; METALLURGY
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/32Amino acids
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/40Nucleotides, nucleosides or bases
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/44Thiols, e.g. mercaptoethanol
    • CCHEMISTRY; METALLURGY
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/46Amines, e.g. putrescine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/11Epidermal growth factor [EGF]
    • CCHEMISTRY; METALLURGY
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/38Hormones with nuclear receptors
    • C12N2501/39Steroid hormones
    • CCHEMISTRY; METALLURGY
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80Neurotransmitters; Neurohormones
    • C12N2501/81Adrenaline
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999Small molecules not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00Genetically modified cells
    • C12N2510/04Immortalised cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69611894T 1995-12-21 1996-12-19 Unsterbliche humane Hautzell-Linien und serumfreies Medium für ihre Herstellung Expired - Lifetime DE69611894T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US57648395A 1995-12-21 1995-12-21

Publications (2)

Publication Number Publication Date
DE69611894D1 DE69611894D1 (de) 2001-04-05
DE69611894T2 true DE69611894T2 (de) 2001-09-13

Family

ID=24304605

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69611894T Expired - Lifetime DE69611894T2 (de) 1995-12-21 1996-12-19 Unsterbliche humane Hautzell-Linien und serumfreies Medium für ihre Herstellung

Country Status (23)

Country Link
US (2) US6423540B2 (enExample)
EP (2) EP0877797A1 (enExample)
JP (1) JP4404965B2 (enExample)
KR (1) KR100455006B1 (enExample)
CN (1) CN1154717C (enExample)
AT (1) ATE199390T1 (enExample)
AU (1) AU730222B2 (enExample)
BR (1) BR9612256B1 (enExample)
CA (1) CA2234118C (enExample)
CZ (1) CZ297289B6 (enExample)
DE (1) DE69611894T2 (enExample)
DK (1) DK0780469T3 (enExample)
ES (1) ES2155166T3 (enExample)
GR (1) GR3035719T3 (enExample)
HU (1) HU226195B1 (enExample)
IL (1) IL124191A (enExample)
NO (1) NO326190B1 (enExample)
NZ (1) NZ325814A (enExample)
PL (1) PL195108B1 (enExample)
PT (1) PT780469E (enExample)
RU (1) RU2215030C2 (enExample)
SK (1) SK283314B6 (enExample)
WO (1) WO1997023602A1 (enExample)

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PT780469E (pt) * 1995-12-21 2001-06-29 Nestle Sa Linhas imortalizadas de celulas derivadas de tecidos cutaneos humanos normais e meios de cultura sem soro adaptados a sua cultura
EP1071747B1 (fr) * 1998-04-17 2004-08-18 Societe Des Produits Nestle S.A. Lignee de cellules immortalisees derivees de tissus cutanes humains normaux
US6964869B2 (en) 1998-07-13 2005-11-15 Wisconsin Alumni Research Foundation Method and composition for skin grafts
CN1087778C (zh) * 1999-03-02 2002-07-17 华东理工大学 杂交瘤细胞无血清培养基
DE19912798C1 (de) 1999-03-10 2000-02-17 Andreas Jordan Verfahren zur Kultivierung von Krebszellen aus Humangewebe und Vorrichtung zur Aufbereitung von Gewebeproben
AUPR298901A0 (en) 2001-02-07 2001-03-08 McComb Foundation, Inc., The Cell suspension preparation technique and device
EP1390754B1 (en) * 2001-04-24 2007-08-01 Wisconsin Alumni Research Foundation Method and composition for skin grafts
US7262174B2 (en) 2001-05-09 2007-08-28 Geron Corporation Treatment for wounds
US20030022369A1 (en) * 2001-05-18 2003-01-30 Helen Fillmore Differentiation of specialized dermal and epidermal cells into neuronal cells
EP1417629B1 (en) * 2001-07-06 2008-02-20 Lipomics Technologies, Inc. Generating, viewing, interpreting, and utilizing a quantitative database of metabolites
GB0208041D0 (en) 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
US20050025737A1 (en) * 2003-07-30 2005-02-03 Sebagh Jean Louis Compositions containing melon extracts
KR20060076781A (ko) * 2003-09-18 2006-07-04 레이븐 바이오테크놀로지스, 인코퍼레이티드 세포 배양 배지
WO2005071065A1 (en) * 2004-01-26 2005-08-04 Cognis France S.A.S Method for studying functional interactions between sensory neurons and keratinocytes or melanocytes
US9295543B2 (en) 2005-03-17 2016-03-29 Stratatech Corporation Skin substitutes with improved purity
EP1818392B1 (en) * 2006-02-14 2010-08-25 Genetix Limited Cell culture medium
WO2010065907A2 (en) * 2008-12-05 2010-06-10 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state
WO2010134619A1 (ja) * 2009-05-18 2010-11-25 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
KR101768632B1 (ko) * 2011-05-18 2017-08-17 (주)아모레퍼시픽 칼슘 펄스를 통한 배양상피세포층의 분리방법
HUE034396T2 (en) 2011-07-12 2018-02-28 Foodchek Systems Inc Culture media, Method for Salmonella and E. coli cultivation and Method for Detection of Salmonella and E. coli
WO2013129885A1 (ko) * 2012-02-28 2013-09-06 건국대학교 산학협력단 세포 배양액
EP2975117B1 (en) 2013-03-11 2018-01-10 JCR Pharmaceuticals Co., Ltd. Method for producing human corneal epithelium sheet
AU2013205148B2 (en) 2013-03-14 2014-10-30 AVITA Medical Americas, LLC Systems and methods for tissue processing and preparation of cell suspension therefrom
WO2016085304A1 (ko) * 2014-11-28 2016-06-02 (주)아모레퍼시픽 멜라닌 세포주 계대배양계 및 이를 이용한 노화 수준 판단, 미용 정보 제공, 또는 스크리닝 방법
CA2971150A1 (en) * 2015-01-26 2016-08-04 Halliburton Energy Services, Inc. Rotating superhard cutting element
CN105238739A (zh) * 2015-11-11 2016-01-13 朱宁文 临床治疗级细胞治疗用黑色素细胞(melanocytes)规模化制备人类细胞外基质筛选培养方法
CN106520674A (zh) * 2016-12-24 2017-03-22 严志海 一种用于表皮黑素细胞体外培养的无血清培养基
CN107115219A (zh) * 2017-05-12 2017-09-01 青岛赛欧凯普图乐生物医药有限公司 一种纤维芽母细胞活化液面膜配方
CN114058565A (zh) * 2020-07-31 2022-02-18 上海尚瑞生物医药科技有限公司 一种无血清的黑素母细胞培养液及其培养方法
KR102583179B1 (ko) * 2020-11-27 2023-09-26 (주)엑셀세라퓨틱스 칼슘, 상피세포성장인자 및 알부민을 포함하는 각질형성세포 수립 또는 배양용 배지 조성물
WO2024145258A1 (en) 2022-12-27 2024-07-04 AVITA Medical Americas, LLC System for automated preparation of a regenerative epidermal suspension and related methods of use
CN119684410B (zh) * 2025-02-21 2025-04-25 深圳市容大生物科技股份有限公司 一种小分子多功能信息肽及其应用

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US4940666A (en) * 1983-07-15 1990-07-10 University Patents, Inc. Process and defined medium for growth of human epidermal keratinocyte cells
US4885238A (en) 1987-10-30 1989-12-05 The United States Of America As Represented By The Department Of Health And Human Services Immortalized human bronchial epitherial mesothelial cell lines
US5292655A (en) * 1990-01-29 1994-03-08 Wille Jr John J Method for the formation of a histologically-complete skin substitute
US5376542A (en) 1992-04-27 1994-12-27 Georgetown University Method for producing immortalized cell lines using human papilluma virus genes
AU2385795A (en) 1994-04-12 1995-10-30 Alza Corporation Screening methods for integumental inflammation modulating agents
DE19617261A1 (de) * 1995-04-21 1996-11-28 Naeher Helmut Dr Med Priv Doz Hautverträglichkeitstest
PT780469E (pt) * 1995-12-21 2001-06-29 Nestle Sa Linhas imortalizadas de celulas derivadas de tecidos cutaneos humanos normais e meios de cultura sem soro adaptados a sua cultura

Also Published As

Publication number Publication date
CA2234118C (en) 2009-04-07
CZ194598A3 (cs) 1998-11-11
US20020042129A1 (en) 2002-04-11
WO1997023602A1 (en) 1997-07-03
PT780469E (pt) 2001-06-29
KR100455006B1 (ko) 2005-01-13
AU730222B2 (en) 2001-03-01
JP2000506374A (ja) 2000-05-30
AU1305497A (en) 1997-07-17
US6949381B2 (en) 2005-09-27
CN1205737A (zh) 1999-01-20
NO326190B1 (no) 2008-10-13
SK83598A3 (en) 1999-01-11
NZ325814A (en) 2000-10-27
EP0780469A1 (fr) 1997-06-25
RU2215030C2 (ru) 2003-10-27
GR3035719T3 (en) 2001-07-31
NO982810D0 (no) 1998-06-18
EP0780469B1 (fr) 2001-02-28
KR19990071817A (ko) 1999-09-27
IL124191A (en) 2004-06-01
NO982810L (no) 1998-08-21
ATE199390T1 (de) 2001-03-15
US20020012993A1 (en) 2002-01-31
CA2234118A1 (en) 1997-07-03
HUP9903742A3 (en) 2000-10-30
JP4404965B2 (ja) 2010-01-27
SK283314B6 (sk) 2003-05-02
PL327320A1 (en) 1998-12-07
EP0877797A1 (en) 1998-11-18
ES2155166T3 (es) 2001-05-01
BR9612256A (pt) 1999-07-13
DK0780469T3 (da) 2001-03-26
CZ297289B6 (cs) 2006-10-11
PL195108B1 (pl) 2007-08-31
DE69611894D1 (de) 2001-04-05
HU226195B1 (hu) 2008-06-30
HUP9903742A2 (hu) 2000-03-28
BR9612256B1 (pt) 2010-08-10
CN1154717C (zh) 2004-06-23
US6423540B2 (en) 2002-07-23

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