DE69534822T2 - Verfahren zur bakteriellen herstellung von heterologen polypeptiden mit disulfidbrücke - Google Patents
Verfahren zur bakteriellen herstellung von heterologen polypeptiden mit disulfidbrücke Download PDFInfo
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- DE69534822T2 DE69534822T2 DE69534822T DE69534822T DE69534822T2 DE 69534822 T2 DE69534822 T2 DE 69534822T2 DE 69534822 T DE69534822 T DE 69534822T DE 69534822 T DE69534822 T DE 69534822T DE 69534822 T2 DE69534822 T2 DE 69534822T2
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- polypeptide
- nucleic acid
- dsba
- heterologous polypeptide
- cells
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- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229910052722 tritium Inorganic materials 0.000 description 1
- 108010087967 type I signal peptidase Proteins 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US333912 | 1994-11-03 | ||
| US08/333,912 US5639635A (en) | 1994-11-03 | 1994-11-03 | Process for bacterial production of polypeptides |
| PCT/US1995/014623 WO1996014422A1 (en) | 1994-11-03 | 1995-10-11 | Process for bacterial production of heterologous polypeptides with disulphide bonds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69534822D1 DE69534822D1 (de) | 2006-04-27 |
| DE69534822T2 true DE69534822T2 (de) | 2006-10-19 |
Family
ID=23304776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69534822T Expired - Lifetime DE69534822T2 (de) | 1994-11-03 | 1995-10-11 | Verfahren zur bakteriellen herstellung von heterologen polypeptiden mit disulfidbrücke |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5639635A (ja) |
| EP (1) | EP0786009B1 (ja) |
| JP (1) | JP3749257B2 (ja) |
| AT (1) | ATE318917T1 (ja) |
| CA (1) | CA2203373C (ja) |
| DE (1) | DE69534822T2 (ja) |
| DK (1) | DK0786009T3 (ja) |
| ES (1) | ES2259440T3 (ja) |
| IL (1) | IL115834A (ja) |
| MX (1) | MX9703012A (ja) |
| WO (1) | WO1996014422A1 (ja) |
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Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL86455A0 (en) * | 1987-06-01 | 1988-11-15 | Takeda Chemical Industries Ltd | Polypeptide and production thereof |
| JPH0638771A (ja) * | 1990-10-31 | 1994-02-15 | Tonen Corp | ヒトプロテインジスルフィドイソメラーゼ遺伝子の発現方法および該遺伝子との共発現によるポリペプチドの製造方法 |
| US5270181A (en) * | 1991-02-06 | 1993-12-14 | Genetics Institute, Inc. | Peptide and protein fusions to thioredoxin and thioredoxin-like molecules |
| US5292646A (en) * | 1991-02-06 | 1994-03-08 | Genetics Institute, Inc. | Peptide and protein fusions to thioredoxin and thioredoxin-like molecules |
| EP0509841A3 (en) * | 1991-04-18 | 1993-08-18 | Tonen Corporation | Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system |
| DE4113750A1 (de) * | 1991-04-26 | 1992-10-29 | Boehringer Mannheim Gmbh | Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen |
| US6291205B1 (en) * | 1992-06-12 | 2001-09-18 | Merck & Co., Inc. | Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae |
| EP0663010B1 (en) * | 1992-10-02 | 2000-08-09 | Research Corporation Technologies, Inc. | Methods for increasing secretion of overexpressed proteins |
-
1994
- 1994-11-03 US US08/333,912 patent/US5639635A/en not_active Expired - Lifetime
-
1995
- 1995-10-11 ES ES95939877T patent/ES2259440T3/es not_active Expired - Lifetime
- 1995-10-11 CA CA002203373A patent/CA2203373C/en not_active Expired - Lifetime
- 1995-10-11 JP JP51488896A patent/JP3749257B2/ja not_active Expired - Lifetime
- 1995-10-11 DE DE69534822T patent/DE69534822T2/de not_active Expired - Lifetime
- 1995-10-11 EP EP95939877A patent/EP0786009B1/en not_active Expired - Lifetime
- 1995-10-11 AT AT95939877T patent/ATE318917T1/de active
- 1995-10-11 WO PCT/US1995/014623 patent/WO1996014422A1/en active IP Right Grant
- 1995-10-11 MX MX9703012A patent/MX9703012A/es unknown
- 1995-10-11 DK DK95939877T patent/DK0786009T3/da active
- 1995-10-31 IL IL11583495A patent/IL115834A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| IL115834A0 (en) | 1996-01-19 |
| JPH10508203A (ja) | 1998-08-18 |
| ES2259440T3 (es) | 2006-10-01 |
| JP3749257B2 (ja) | 2006-02-22 |
| WO1996014422A1 (en) | 1996-05-17 |
| CA2203373A1 (en) | 1996-05-17 |
| IL115834A (en) | 2004-08-31 |
| CA2203373C (en) | 2007-08-28 |
| DK0786009T3 (da) | 2006-07-03 |
| US5639635A (en) | 1997-06-17 |
| ATE318917T1 (de) | 2006-03-15 |
| EP0786009B1 (en) | 2006-03-01 |
| DE69534822D1 (de) | 2006-04-27 |
| EP0786009A1 (en) | 1997-07-30 |
| MX9703012A (es) | 1997-07-31 |
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