DE69432543D1 - Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme - Google Patents

Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme

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Publication number
DE69432543D1
DE69432543D1 DE69432543T DE69432543T DE69432543D1 DE 69432543 D1 DE69432543 D1 DE 69432543D1 DE 69432543 T DE69432543 T DE 69432543T DE 69432543 T DE69432543 T DE 69432543T DE 69432543 D1 DE69432543 D1 DE 69432543D1
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Germany
Prior art keywords
strains
selection marker
preparation
marker gene
dna
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Expired - Lifetime
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DE69432543T
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English (en)
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DE69432543T2 (de
Inventor
Gerardus Cornelis Maria Selten
Gorcom Robertus Franciscus Van
Bart Willem Swinkels
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DSM IP Assets BV
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DSM NV
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Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69432543T 1993-07-23 1994-06-30 Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme Expired - Lifetime DE69432543T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP93202195 1993-07-23

Publications (2)

Publication Number Publication Date
DE69432543D1 true DE69432543D1 (de) 2003-05-28
DE69432543T2 DE69432543T2 (de) 2003-12-24

Family

ID=8214006

Family Applications (1)

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DE69432543T Expired - Lifetime DE69432543T2 (de) 1993-07-23 1994-06-30 Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme

Country Status (13)

Country Link
US (3) US5876988A (de)
EP (2) EP0635574B1 (de)
JP (2) JP3833725B2 (de)
CN (1) CN100347301C (de)
AT (1) ATE238425T1 (de)
BR (1) BR9402912A (de)
CA (1) CA2128589C (de)
DE (1) DE69432543T2 (de)
DK (1) DK0635574T3 (de)
ES (1) ES2198410T3 (de)
FI (2) FI114481B (de)
IE (1) IE20030875A1 (de)
NZ (2) NZ299139A (de)

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DE69432543T2 (de) 2003-12-24
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FI943485A (fi) 1995-01-24
US5876988A (en) 1999-03-02
CN100347301C (zh) 2007-11-07
DK0635574T3 (da) 2003-08-11
JP3833725B2 (ja) 2006-10-18
US6063614A (en) 2000-05-16
IE20030875A1 (en) 2004-01-14
CN1098743A (zh) 1995-02-15
JPH07147971A (ja) 1995-06-13
AU690909B2 (en) 1998-05-07
US6955909B1 (en) 2005-10-18
EP1321523A3 (de) 2004-03-03
IE940591A1 (en) 1995-01-25
FI117388B (fi) 2006-09-29
FI20041124A (fi) 2004-08-27
AU6862894A (en) 1995-02-02
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NZ299139A (en) 1998-06-26
EP1321523A2 (de) 2003-06-25
CA2128589C (en) 2005-10-11
BR9402912A (pt) 1995-04-11
EP0635574B1 (de) 2003-04-23
FI114481B (fi) 2004-10-29
ATE238425T1 (de) 2003-05-15
NZ264065A (en) 1998-06-26
CA2128589A1 (en) 1995-01-24

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