DE68928413D1 - Verfahren zur dns-sequenzbestimmung mit dns-polymerase von thermus aquaticus - Google Patents

Verfahren zur dns-sequenzbestimmung mit dns-polymerase von thermus aquaticus

Info

Publication number
DE68928413D1
DE68928413D1 DE68928413T DE68928413T DE68928413D1 DE 68928413 D1 DE68928413 D1 DE 68928413D1 DE 68928413 T DE68928413 T DE 68928413T DE 68928413 T DE68928413 T DE 68928413T DE 68928413 D1 DE68928413 D1 DE 68928413D1
Authority
DE
Germany
Prior art keywords
thermus aquaticus
dna polymerase
determining
dna sequence
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68928413T
Other languages
English (en)
Other versions
DE68928413T2 (de
Inventor
Michael Innis
Kenneth Myambo
David Gelfand
Mary Brow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22943169&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE68928413(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of DE68928413D1 publication Critical patent/DE68928413D1/de
Publication of DE68928413T2 publication Critical patent/DE68928413T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE68928413T 1988-09-23 1989-09-19 Verfahren zur dns-sequenzbestimmung mit dns-polymerase von thermus aquaticus Expired - Lifetime DE68928413T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/249,367 US5075216A (en) 1988-09-23 1988-09-23 Methods for dna sequencing with thermus aquaticus dna polymerase
PCT/US1989/004093 WO1990003442A1 (en) 1988-09-23 1989-09-19 Methods for dna sequencing with thermus aquaticus dna polymerase

Publications (2)

Publication Number Publication Date
DE68928413D1 true DE68928413D1 (de) 1997-12-04
DE68928413T2 DE68928413T2 (de) 1998-06-04

Family

ID=22943169

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68928413T Expired - Lifetime DE68928413T2 (de) 1988-09-23 1989-09-19 Verfahren zur dns-sequenzbestimmung mit dns-polymerase von thermus aquaticus

Country Status (8)

Country Link
US (1) US5075216A (de)
EP (1) EP0437459B1 (de)
JP (1) JP2901194B2 (de)
AT (1) ATE159762T1 (de)
AU (1) AU647015B2 (de)
CA (1) CA1332561C (de)
DE (1) DE68928413T2 (de)
WO (1) WO1990003442A1 (de)

Families Citing this family (147)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5333675C1 (en) * 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5656493A (en) * 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US5173411A (en) * 1987-01-14 1992-12-22 President And Fellows Of Harvard College Method for determining the nucleotide base sequence of a DNA molecule
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
KR100236506B1 (ko) * 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
DE4214112A1 (de) * 1991-08-02 1993-02-04 Europ Lab Molekularbiolog Neues verfahren zur sequenzierung von nukleinsaeuren
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US5830642A (en) * 1992-04-03 1998-11-03 Amersham Life Science, Inc. Electrophoresis of nucleic acid fragments
US5314595A (en) * 1992-04-03 1994-05-24 United States Biochemical Corporation Electrophoresis of nucleic acid fragments
US6410277B1 (en) 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US7465539B1 (en) 1993-02-19 2008-12-16 Takara Bio, Inc. DNA polymerases with enhanced length of primer extension
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5550040A (en) * 1993-06-23 1996-08-27 Hoffman-La Roche Inc. Method, reagents and kits for the detection of Neisseria gonorrhoeae
US5601990A (en) * 1994-09-13 1997-02-11 Thomas Jefferson University Methods of diagnosing colorectal tumors and metastasis thereof
US7097839B1 (en) 1993-10-26 2006-08-29 Thomas Jefferson University ST receptor binding compounds and methods of using the same
JP4194113B2 (ja) * 1993-10-26 2008-12-10 トーマス・ジェファーソン・ユニバーシティ 結腸直腸癌細胞に特異的に結合する組成物およびその使用方法
US5879656A (en) * 1993-10-26 1999-03-09 Thomas Jefferson University Methods of treating metastatic colorectal cancer with ST receptor binding compounds
US7312052B1 (en) * 1993-12-08 2007-12-25 Stratagene California Polymerase compositions and uses thereof
US5480783A (en) * 1994-03-31 1996-01-02 The Perkin-Elmer Corporation Method for reducing background signals in DNA replication/detection assays
US6455251B1 (en) 1994-09-13 2002-09-24 Thomas Jefferson University Methods of and kits and compositions for diagnosing colorectal tumors and metastasis thereof
US5919617A (en) * 1994-12-21 1999-07-06 Miami University Methods and reagents for detecting fungal pathogens in a biological sample
JP3966555B2 (ja) * 1995-05-31 2007-08-29 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 熱耐性ｄｎａポリメラーゼ
US5876949A (en) * 1995-05-31 1999-03-02 The Trustees Of The University Of Pennsylvania Antibodies specific for fragile X related proteins and method of using the same
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5750345A (en) * 1995-10-31 1998-05-12 Evanston Hospital Corporation Detection of human α-thalassemia mutations and their use as predictors of blood-related disorders
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5888736A (en) * 1995-12-22 1999-03-30 Visible Genetics, Inc. Method, compositions and kit for detection and identification of microorganisms
US6168918B1 (en) 1996-01-31 2001-01-02 American Home Products Corp. Method of detecting foreign DNA integrated in eukaryotic chromosomes
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
WO1997042506A1 (en) 1996-05-03 1997-11-13 Thomas Jefferson University Methods of and kits and compositions for diagnosing colorectal tumors and metastasis thereof
US5910409A (en) * 1996-05-20 1999-06-08 Miami University Methods and reagents for detecting fungal pathogens in a biological sample
US6455248B1 (en) 1996-05-20 2002-09-24 Miami University Reagents and kits for detecting fungal pathogens in a biological sample
US5849546A (en) * 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US6139833A (en) * 1997-08-08 2000-10-31 Lexicon Genetics Incorporated Targeted gene discovery
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US6855545B1 (en) 1996-10-04 2005-02-15 Lexicon Genetics Inc. Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6207371B1 (en) 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
DE19653439A1 (de) * 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
DE19653494A1 (de) * 1996-12-20 1998-06-25 Svante Dr Paeaebo Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
US6605428B2 (en) 1996-12-20 2003-08-12 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
US5952200A (en) * 1997-02-06 1999-09-14 University Of South Carolina Method of diagnosing cancer in human cells using a reverse transcriptase-polymerase chain reaction for identifying the presence of stromelysin-3
WO1998035707A1 (en) 1997-02-18 1998-08-20 Thomas Jefferson University Compositions that bind to pancreatic cancer cells and methods of using the same
CA2283635A1 (en) 1997-03-21 1998-10-01 Stratagene Polymerase enhancing factor (pef) extracts, pef protein complexes, isolated pef protein, and methods for purifying and identifying
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
AU746892B2 (en) * 1997-04-04 2002-05-02 Caliper Life Sciences, Inc. Closed-loop biochemical analyzers
US6120995A (en) * 1997-08-07 2000-09-19 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US7135333B1 (en) 1997-08-07 2006-11-14 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
CA2318880C (en) * 1998-02-04 2010-07-27 Perkin-Elmer Corporation Determination of a genotype of an amplification product at multiple allelic sites
US6808921B1 (en) * 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US20020064837A1 (en) * 1998-05-08 2002-05-30 Thuan Quoc Trinh Method for synthesizing a nucleic acid molecule using a ribonuclease
JP2002524053A (ja) 1998-08-06 2002-08-06 デューク・ユニバーシティー 尿酸オキシダーゼ
US6333403B1 (en) * 1998-11-06 2001-12-25 Myriad Genetics, Inc. Chromosome 17p-linked prostate cancer susceptibility gene and a paralog and orthologous genes
EP1074633A1 (de) * 1999-07-05 2001-02-07 LION Bioscience AG Nukleotide und ihre Verwendung in der Sequenzierung von GC-reichen Templaten
GB9922971D0 (en) * 1999-09-29 1999-12-01 Secr Defence Reaction system
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US6893819B1 (en) * 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
WO2001061015A2 (de) 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
CA2404432A1 (en) 2000-03-27 2001-10-04 Thomas Jefferson University Compositions and methods for identifying and targeting cancer cells
EP1182267B1 (de) * 2000-03-30 2012-01-18 Toyota Jidosha Kabushiki Kaisha Verfahren zur bestimmung der basensequenz eines einzelnen nukleinsäuremoleküls
US20080242627A1 (en) * 2000-08-02 2008-10-02 University Of Southern California Novel rna interference methods using dna-rna duplex constructs
US7662791B2 (en) 2000-08-02 2010-02-16 University Of Southern California Gene silencing using mRNA-cDNA hybrids
WO2002022885A1 (en) * 2000-09-18 2002-03-21 Thomas Jefferson University Compositions and methods for identifying and targeting stomach and esophageal cancer cells
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
GB0110476D0 (en) 2001-04-30 2001-06-20 Secr Defence Reagent delivery system
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
WO2003040376A1 (en) * 2001-11-02 2003-05-15 Olga Makarova Method for site-directed mutagenesis of nucleic acid molecules using a single primer
US7222059B2 (en) * 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
CA2477761A1 (en) * 2002-03-01 2003-09-12 Ravgen, Inc. Methods for detection of genetic disorders
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
ATE397096T1 (de) * 2002-03-20 2008-06-15 Innovativebio Biz Mikrokapseln mit kontrollierter durchlässigkeit die ein nukleinsäureamplifizierungsreaktionsgemisch enthalten und deren benutzung als reaktionsgefäss für parallele reaktionen
US20030228611A1 (en) * 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
US7727720B2 (en) 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US7442506B2 (en) 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20030210489A1 (en) * 2002-05-13 2003-11-13 Yec Co., Ltd. Data eraser and data erasing program
EP2194123B1 (de) 2003-03-25 2012-08-22 Stratagene California DNA-Polymerasefusionen und Verwendungen dafür
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
WO2004094659A1 (en) 2003-04-21 2004-11-04 Roche Diagnostics Gmbh Associations of polymorphisms in the frzb gene with obesity and osteoporosis
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20070059701A1 (en) * 2003-06-30 2007-03-15 Astrazeneca Ab Fluorescence assays for nucleic acid polymerase activity
US7179602B2 (en) * 2003-09-04 2007-02-20 Los Alamos National Security, Llc Methods for sequencing GC-rich and CCT repeat DNA templates
RU2006114432A (ru) * 2003-09-30 2007-11-20 Лексикон Дженетикс Инкорпорейтед (Us) Способы и композиции для определения функции гена
EP1706502A4 (de) * 2003-11-26 2007-05-23 Eppendorf Ag Verfahren und zusammensetzungen zur in-vitro-amplifikation extrachromosomaler nukleinsäure
US8293471B2 (en) * 2004-01-28 2012-10-23 Marshall University Research Corporation Apparatus and method for a continuous rapid thermal cycle system
WO2006029184A2 (en) 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
JP2008518621A (ja) * 2004-11-03 2008-06-05 サード・ウェーブ・テクノロジーズ・インク 一段階検出アッセイ法
US7051536B1 (en) * 2004-11-12 2006-05-30 Bio-Rad Laboratories, Inc. Thermal cycler with protection from atmospheric moisture
JP2006230342A (ja) * 2005-02-28 2006-09-07 Tokyo Univ Of Agriculture & Technology 一本鎖核酸の分離方法および装置並びにマイクロアレイおよびｄｎａチップ。
GB0514935D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
EP1989318B1 (de) 2006-01-06 2014-07-30 Agilent Technologies, Inc. Reaktionspufferzusammensetzung für die nukleinsäurereplikation mit gepackten dna-polymerasen
WO2007087690A1 (en) 2006-02-02 2007-08-09 Corbett Life Science Pty Ltd Thermocycler and sample port
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
US7993832B2 (en) 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
WO2008140484A2 (en) 2006-11-09 2008-11-20 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
JP5225294B2 (ja) * 2007-03-02 2013-07-03 コーベット リサーチ プロプライエタリー リミテッド 核酸増幅のための装置および方法
CN101801514A (zh) * 2007-08-28 2010-08-11 考贝特研究控股公司 带有可选择性的打开的试样端口的热循环装置
EP2227559B1 (de) 2007-11-30 2018-08-29 QIAGEN Instruments AG Vorrichtung für thermisches cycling
CA2715170A1 (en) * 2008-02-11 2009-08-20 Hadasit Medical Research Services & Development Limited Colon cancer associated transcript 1 (ccat-1) as a cancer marker
AU2009225835B2 (en) * 2008-03-15 2013-02-14 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
EP2316030B1 (de) 2008-07-25 2019-08-21 Wagner, Richard W. Proteinscreeningverfahren
CA2638458A1 (en) * 2008-07-31 2010-01-31 Spartan Bioscience Inc. Thermal recycling by positioning relative to fixed-temperature source
WO2010033604A2 (en) * 2008-09-18 2010-03-25 X-Bar Diagnostic System, Inc. Fully automated portable dna detection system
US9023633B2 (en) 2008-11-03 2015-05-05 Kapa Biosystems Chimeric DNA polymerases
EP3101124B1 (de) 2008-11-03 2019-06-19 Kapa Biosystems, Inc. Modifizierte typ-a-dna-polymerasen
US20110070590A1 (en) 2009-09-22 2011-03-24 Jan Rohozinski Primers and Methods for Determining RhD Zygosity
CN102959088A (zh) 2010-02-02 2013-03-06 艾博特生物技术有限公司 用于预测对TNF-α抑制剂治疗的反应性的方法和组合物
WO2011127136A1 (en) * 2010-04-06 2011-10-13 University Of Chicago Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc)
BR112012026945B1 (pt) 2010-04-20 2021-05-25 Qiagen Gmbh método e aparelho para controlar a temperatura de um líquido
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
ES2731653T3 (es) 2011-03-18 2019-11-18 Eisai R&D Man Co Ltd Métodos y usos para predecir la respuesta a la eribulina
US9976917B2 (en) 2011-10-20 2018-05-22 Qiagen Instruments Ag Method for verifying a temperature measurement in a micro-environment and system for verifying a temperature measurement in a micro-environment
AU2012324458B2 (en) 2011-10-20 2016-05-19 Novartis Ag Biomarkers predictive of responsiveness to alpha 7 nicotinic acetylcholine receptor activator treatment
EP2827991B1 (de) 2012-03-23 2019-05-29 QIAGEN GmbH Thermocycler und verfahren zur erwärmung und kühlung
US20140024541A1 (en) * 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
EP2687294A1 (de) 2012-07-18 2014-01-22 Qiagen Instruments AG Rotor für einen Wärmeumwälzer und Wärmeumwälzer
BR112015013771B1 (pt) 2012-12-11 2022-10-04 Novartis Ag Composição que compreende um ativador do receptor alfa 7, nicotínico de acetilcolina (nachr) como biomarcador preditivo de resposta ao tratamento de ativador do dito receptor
US9963740B2 (en) 2013-03-07 2018-05-08 APDN (B.V.I.), Inc. Method and device for marking articles
EP3058339B1 (de) 2013-10-07 2019-05-22 APDN (B.V.I.) Inc. Multimodales bild und spektraler leser
AU2014336962B2 (en) 2013-10-15 2019-07-25 Bio Molecular Systems Pty Ltd Improved thermocycler
EP3102701A1 (de) 2014-02-07 2016-12-14 Novartis AG Auswirkung von genetische faktoren auf die krankheitsprogression und reaktion auf anti-c5-antikörper bei geografischer atrophie
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
CA2940655C (en) 2014-03-18 2020-07-07 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
US10851428B2 (en) 2015-09-18 2020-12-01 Siemens Healthcare Diagnostics Inc. Reagents and methods for analysis of HIV
EP3387107B1 (de) 2015-12-11 2020-08-12 Spartan Bioscience Inc. Röhrchenverschlusssystem und verfahren zur nukleinsäureamplifikation
US10519605B2 (en) 2016-04-11 2019-12-31 APDN (B.V.I.), Inc. Method of marking cellulosic products
JP6987134B2 (ja) 2016-06-27 2021-12-22 ジュノー セラピューティクス インコーポレイテッド ペプチドエピトープを同定する方法、そのようなエピトープに結合する分子および関連用途
MA45491A (fr) 2016-06-27 2019-05-01 Juno Therapeutics Inc Épitopes à restriction cmh-e, molécules de liaison et procédés et utilisations associés
CN109476695A (zh) 2016-06-27 2019-03-15 丹娜法伯癌症研究院 用于测定rna翻译速率的方法
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication
WO2019089982A1 (en) 2017-11-01 2019-05-09 Juno Therapeutics, Inc. Method of assessing activity of recombinant antigen receptors
CA3223867A1 (en) 2021-06-30 2023-01-05 Gerassimos Makrigiorgos Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1339653C (en) * 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US4921794A (en) * 1987-01-14 1990-05-01 President And Fellows Of Harvard College T7 DNA polymerase
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
DE69002826T2 (de) * 1989-02-06 1994-03-31 Eastman Kodak Co Reaktionskonzentrat zur dna-sequenzierung mit thermostabiler dna-polymerase.
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase

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DE68928413T2 (de) 1998-06-04
ATE159762T1 (de) 1997-11-15
US5075216A (en) 1991-12-24
EP0437459A1 (de) 1991-07-24
JPH04501205A (ja) 1992-03-05
CA1332561C (en) 1994-10-18
AU4300389A (en) 1990-04-18
AU647015B2 (en) 1994-03-17
JP2901194B2 (ja) 1999-06-07
EP0437459B1 (de) 1997-10-29

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