ATE250142T1 - Methode zur sequenzierung von polynukleotiden - Google Patents

Methode zur sequenzierung von polynukleotiden

Info

Publication number
ATE250142T1
ATE250142T1 AT00910209T AT00910209T ATE250142T1 AT E250142 T1 ATE250142 T1 AT E250142T1 AT 00910209 T AT00910209 T AT 00910209T AT 00910209 T AT00910209 T AT 00910209T AT E250142 T1 ATE250142 T1 AT E250142T1
Authority
AT
Austria
Prior art keywords
target
primer
extension products
labeled
hybrid
Prior art date
Application number
AT00910209T
Other languages
English (en)
Inventor
Malcolm D Mcginnis
Alex M Madonik
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE250142T1 publication Critical patent/ATE250142T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT00910209T 1999-02-16 2000-02-16 Methode zur sequenzierung von polynukleotiden ATE250142T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/250,422 US6635419B1 (en) 1999-02-16 1999-02-16 Polynucleotide sequencing method
PCT/US2000/004034 WO2000049178A2 (en) 1999-02-16 2000-02-16 Polynucleotide sequencing method

Publications (1)

Publication Number Publication Date
ATE250142T1 true ATE250142T1 (de) 2003-10-15

Family

ID=22947677

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00910209T ATE250142T1 (de) 1999-02-16 2000-02-16 Methode zur sequenzierung von polynukleotiden

Country Status (8)

Country Link
US (2) US6635419B1 (de)
EP (1) EP1159454B1 (de)
JP (1) JP4113675B2 (de)
AT (1) ATE250142T1 (de)
AU (1) AU764099B2 (de)
CA (1) CA2362245C (de)
DE (1) DE60005309T2 (de)
WO (1) WO2000049178A2 (de)

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US6635419B1 (en) * 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
EP1776482A2 (de) 2004-06-30 2007-04-25 Applera Corporation Loglineare verstärkung
FI20075124A0 (fi) * 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
EP2235210B1 (de) 2007-12-21 2015-03-25 President and Fellows of Harvard College Verfahren zur nukleinsäuresequenzierung
WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3290531B1 (de) 2008-12-19 2019-07-24 President and Fellows of Harvard College Partikelunterstützte nukleinsäuresequenzierung
EP3842150A1 (de) 2009-10-27 2021-06-30 President and Fellows of Harvard College Tröpfchenbildungsverfahren
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
AU2015279546B2 (en) 2014-06-26 2021-04-08 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN112126675B (zh) 2015-01-12 2022-09-09 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
EP3256606B1 (de) 2015-02-09 2019-05-22 10X Genomics, Inc. Systeme und verfahren zur bestimmung struktureller varianten
EP4286516A3 (de) 2015-02-24 2024-03-06 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
EP3262188B1 (de) 2015-02-24 2021-05-05 10X Genomics, Inc. Verfahren zur sequenzierung von nukleinsäuren in gesteuertem ausmass
US11123297B2 (en) 2015-10-13 2021-09-21 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
WO2017096158A1 (en) 2015-12-04 2017-06-08 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3545089B1 (de) 2017-01-30 2022-03-09 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
EP3625715A4 (de) 2017-05-19 2021-03-17 10X Genomics, Inc. Systeme und verfahren zur analyse von datensätzen
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CN117795097A (zh) * 2021-09-06 2024-03-29 株式会社日立高新技术 核苷酸序列确定方法

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US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5654442A (en) 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
US5187085A (en) 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
US5210412A (en) * 1991-01-31 1993-05-11 Wayne State University Method for analyzing an organic sample
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
DE69322266T2 (de) 1992-04-03 1999-06-02 Perkin Elmer Corp Proben zusammensetzung und verfahren
US5670346A (en) 1994-03-08 1997-09-23 Amersham International Plc Modifying nucleotide analogues
US5976798A (en) 1994-03-30 1999-11-02 Mitokor Methods for detecting mitochondrial mutations diagnostic for Alzheimer's disease and methods for determining heteroplasmy of mitochondrial nucleic acid
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US6635419B1 (en) * 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method

Also Published As

Publication number Publication date
US6635419B1 (en) 2003-10-21
AU764099B2 (en) 2003-08-07
CA2362245A1 (en) 2000-08-24
EP1159454A2 (de) 2001-12-05
DE60005309D1 (de) 2003-10-23
JP4113675B2 (ja) 2008-07-09
WO2000049178A2 (en) 2000-08-24
WO2000049178A3 (en) 2000-12-14
EP1159454B1 (de) 2003-09-17
JP2002536980A (ja) 2002-11-05
CA2362245C (en) 2008-12-09
US20040063138A1 (en) 2004-04-01
DE60005309T2 (de) 2004-06-24
AU3233900A (en) 2000-09-04

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